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Agrawal S.S.,Delhi Institute of Pharmaceutical science and Research DIPSAR
Indian Journal of Natural Products and Resources

Zuroor-e-Qula, a powdered polyherbal Unani formulation, known to possess antimicrobial and anti-inflammatory properties is recommended in cases of stomatitis and gastric ulceration. The freshly prepared formulation was evaluated for its mineral contents, microbial count, aflatoxins and pesticide residues. The results revealed that this formulation is free from such contaminants and its use is safe. © 2014 National Institute of Science Communication and Information Resources (NISCAIR). All rights reserved. Source

Nanda S.,iversity | Pathak D.P.,Delhi Institute of Pharmaceutical science and Research DIPSAR
Medicinal Chemistry Research

In present research work, we report the synthesis, in vitro hydrolysis study and pharmacological evaluation of new mutual prodrugs of mefenamic acid (MA) and 1,2 dihydro-1,5-dimethyl-4-(1-methylethyl)-2-phenylpyrazol-3-one with the aim of improving the therapeutic potency and retard the adverse effects of gastrointestinal origin. The structure of the synthesized mutual ester prodrugs (MP1 and MP2) were confirmed by IR, 1H NMR, 13C NMR, mass spectroscopy and their formation was confirmed by TLC. The purity of the synthesized compounds was established by elemental analysis. The title compounds were tested for analgesic activity by acetic acid-induced writhing method; anti-inflammatory activity was tested by carrageenan-induced rat paw edema method and ulcerogenicity. The study of the analgesic activity revealed that both prodrugs (MP1 and MP2) have shown significant reduction (81.67, 63.90 %) in writhing response produced by acetic acid as compared to parent drug MA (61.10 %). Both mutual prodrugs showed better maximum anti-inflammatory effects (71.43, 85.71 %) and for longer time as compared to parent drug MA (53.14 %). The synthesized prodrugs were also found to be very less irritating to gastric mucosal membrane than parent drugs. The kinetics of ester hydrolysis was studied in simulated gastric fluid (SGF) at pH 1.2 and simulated intestinal fluid (SIF) at pH 7.4. The release of free MA from prodrugs showed negligible hydrolysis in SGF as compared to SIF. This indicated that prodrugs were sufficiently stable at pH 1.2 and do not break in the stomach, but release MA in SIF. © 2013 Springer Science+Business Media New York. Source

Singh A.,R V Northland Institute | Sharma P.K.,Meerut Institute of Engineering and Technology MIET | Majumdar D.K.,Delhi Institute of Pharmaceutical science and Research DIPSAR
Journal of Liquid Chromatography and Related Technologies

In the present work, a new reverse phase-high performance liquid chromatography (RP-HPLC) method was developed, validated, and compared for estimation of the bis-triazole antifungal fluconazole (FLZ), in different simulated biological fluids, that is, gastric fluid simulant (GFS, pH = 1.5), vaginal fluid simulant (VFS, pH = 4.2), topical simulated media-phosphate buffer (PB, pH = 6.8), blood plasma simulant-phosphate buffer saline (PBS, pH = 7.4) and in methanol. For RP-HPLC the chromatography was carried out on a octadecyl silane (ODS-3) Hypersil C18 column (250 mm × 4.6 mm × 5 μm) using water (pH 5.2, adjusted with orthophosphoric acid) and acetonitrile (80:20, v/v) as the mobile phase at a flow rate of 2.5 mL/min with detection at 260 nm. The calibration curve of standard FLZ was linear in the range 0.2-40 μg/mL, 0.1-40 μg/L, 0.2-40 μg/mL, 0.1-40 μg/mL, and 0.2-50 μg/mL, in GFS, VFS, PB, PBS, and in methanol, respectively. All the methods were validated with respect to linearity, precision, accuracy, and specificity for intra- and inter-day and satisfied the International Conference on Harmonisation (ICH) and United States Food and Drug Administration (USFDA'S) guidelines. Copyright © 2014 Taylor & Francis Group, LLC. Source

Agnihotri S.,Delhi Institute of Pharmaceutical science and Research DIPSAR | Wakode S.,Delhi Institute of Pharmaceutical science and Research DIPSAR | Ali M.,Jamia Hamdard University
Natural Product Research

Hydrodistilled oil obtained from the stem bark of Myrica esculenta Buch. Ham. ex D. Don (yield 0.3%) was analysed by capillary GC and GC-MS. The volatile oil consisted mainly of n-hexadecanol (25.2%), eudesmol acetate (21.9%), palmitic acid (11.6%), cis-β-caryophyllene (8.7%), n-pentadecanol (7.7%) and n-octadecanol (7.6%). The oil was found to be a potential antimicrobial agent against Bacillus pumilus, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Aspergillus niger and Saccharomyces cerevisiae. The essential oil exhibited significant topical anti-inflammatory activity compared to standard drug in Swiss albino mice ear. © 2012 Taylor & Francis Group, LLC. Source

Agrawal S.S.,Delhi Institute of Pharmaceutical science and Research DIPSAR | Gullaiya S.,Delhi Institute of Pharmaceutical science and Research DIPSAR | Dubey V.,Delhi Institute of Pharmaceutical science and Research DIPSAR | Singh V.,Delhi Institute of Pharmaceutical science and Research DIPSAR | And 2 more authors.
Cardiovascular Psychiatry and Neurology

Study was undertaken to evaluate the neurodegenerative defending potential of curcumin (CUR), demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC) on 6-hydroxydopamine-(6-OHDA) induced Parkinsonism model in rats. Curcuminoids were administered (60 mg/kg, body weight, per oral) for three weeks followed by unilateral injection of 6-OHDA on 22nd day (10 μg/2 μL) into the right striatum leading to extensive loss of dopaminergic cells. The behavioral observations, biochemical markers, quantification of dopamine (DA), DOPAC, and HVA followed by dopamine (D 2) receptor binding assay and tyrosine hydroxylase (TH, using immunohistochemistry) were evaluated using HPLC after three weeks of lesion. Pretreated animals showed significant protection against neuronal degeneration compared to lesion animals by normalizing the deranged levels of biomarkers and showed the potency in the order CUR > DMC > BDMC. The same order of effectiveness was observed in D 2 receptors binding assay and TH immunohistochemistry study. We conclude that curcuminoids appear to shield progressive neuronal degeneration from increased oxidative attack in 6-OHDA-lesioned rats through its free radical scavenging mechanism, and DA, DOPAC, and HVA enhancing capabilities in the sequence of efficacy CUR > DMC > BDMC. Further, curcuminoids may have potential utility in treatment of many more oxidative stress-induced neurodegenerative disorders. © 2012 Shyam Sunder Agrawal et al. Source

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