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Negi J.S.,Hemwati Nandan Bahuguna Garhwal University | Singh P.,Hemwati Nandan Bahuguna Garhwal University | Nee Pant G.J.,Hemwati Nandan Bahuguna Garhwal University | Rawat M.S.M.,Hemwati Nandan Bahuguna Garhwal University | Pandey H.K.,Defence Agricultural Research Laboratory
Biological Trace Element Research | Year: 2010

The present study was carried out to determine the accumulation and variation of trace elements in roots and leaves of Asparagus racemosus collected from four different altitudes in Uttarakhand, India, by atomic absorption spectroscopy. The metals investigated were Zn, Cu, Mn, Fe, Co, Na, K, Ca, and Li. The concentration level of Fe was found to be highest at an altitude of 2,250 m, whereas the level of Cu was lowest. The maximum concentrations of Zn, Cu, Mn, Fe, Co, Na, K, Ca, and Li were found to be 165.0±3.2, 34.0±0.5, 84.0±0.7, 2,040.0±0.3, 122.0±1.5, 745.0±0.3, 13,260.0±3.5, 6,153.0±1.6, and 58.0±3.8 mg/kg, respectively. © 2009 Humana Press Inc. Source

Anandhan S.,Defence Agricultural Research Laboratory | Sivalingam P.N.,Central Institute for Arid Horticulture | Satheesh V.,National Research Center on Plant Biotechnology | Saritha R.K.,Indian Institute of Vegetable Research | Parameshwari B.,Defence Food Research Laboratory
Archives of Phytopathology and Plant Protection | Year: 2011

The leaf curl disease of tomato was observed in the Haldwani region of Uttarakhand, India during 2004-2007 with an average disease incidence of 49.8 and 73.7% during the month of October and February, respectively. The virus isolate from the infected tomato plants was transmissible to healthy tomato plants by whiteflies (Bemisia tabaci), and the inoculated plants showed typical leaf curl symptoms with a latent period of 16-18 days. The total DNA was extracted from the infected plants and subjected to polymerase chain reaction to amplify the genomic components. The coat protein (CP) gene of ~750 nt was amplified using a set of CP gene specific primer and sequenced (EU847240). Sequence analysis of 701 nt from the N′ terminal region revealed that it had a sequence identity of more than 90% with other isolates/strains of Tomato leaf curl New Delhi virus. A satellite molecule, DNA β of ~1.4 kb was also amplified using universal DNA β-specific primers, cloned and sequenced (EU847239). The isolated DNA β was 1370 nt in length and had a nucleotide sequence identity of 91-93% with DNA β associated with cowpea severe leaf curl and tomato leaf curl disease (TomLCD) reported from India and Pakistan, respectively, and followed by 79% with DNA β associated with TomLCDs reported from Rajasthan. This result showed that the satellite DNA β was associated with TomLCD in Haldwani. © 2011 Copyright Taylor and Francis Group, LLC. Source

Purkayastha J.,Indian Institute of Technology Guwahati | Sugla T.,Indian Institute of Technology Guwahati | Paul A.,Indian Institute of Technology Guwahati | Solleti S.K.,Indian Institute of Technology Guwahati | And 5 more authors.
Biologia Plantarum | Year: 2010

An efficient and reproducible in vitro plant regeneration system from shoot apices was developed in Jatropha curcas. Benzylaminopurine (BAP; 2.5 μM) was most effective in inducing an average of 6.2 shoots per shoot apex. Incorporation of gibberellic acid (GA3; 0.5 μM) to basal medium was found essential for elongation of shoots. The BAP-habituated mother explants continuously produced shoots during successive subculture without any loss of morphogenic potential. The shoots rooted efficiently on half-strength MS medium. The rooted plantlets were acclimatized with more than 98% success and the plants transferred to soil:compost in nursery showed no sign of variation compared to the seed-grown plants. The whole process of culture initiation to plant establishment was accomplished within 5-6 weeks. A genetic transformation system in J. curcas was established for the first time, using bombardment of particles coated with plasmid pBI426 with a GUS-NPT II fusion protein under the control of a double 35S cauliflower mosaic virus (CaMV) promoter. The β-glucuronidase (GUS) activity in J. curcas shoot apices was significantly affected by the gold particle size, bombardment pressure, target distance, macrocarrier travel distance, number of bombardments, and type and duration of osmotic pre-treatment. The proliferating bombarded shoot apices were screened on medium supplemented with 25 mg dm-3 kanamycin and surviving shoots were rooted on medium devoid of kanamycin. The integration of the transgene into genomic DNA of transgenic plants was confirmed by PCR and Southern blot hybridization. The transgenic plants showed insertion of single to multiple copies of the transgene. © Springer Science+Business Media B.V 2010. Source

Gupta S.M.,Defence Agricultural Research Laboratory | Singh R.K.,Defence Agricultural Research Laboratory | Ahmed Z.,Defence Agricultural Research Laboratory
Proceedings of the National Academy of Sciences India Section B - Biological Sciences | Year: 2010

Low temperature stress is an important factor limiting the production and geographic distribution of many horticultural crop species Being multigenic as well as a quantitative trait, it is a challenge to understand the molecular basis of cold stress Cold acclimation in winter wheat is associated with the expression of hundreds of genes that contribute towards freeze tolerance. In the present investigation, the use of mRNA differential display to identify six cold-regulated genes in wheat has been done Transcript analyses show that four genes are up-regulated and two down-regulated during cold stress with varied patterns of transcript accumulation Sequence analyses reveal homology to genes that are involved in diverse processes such as gene regulation/signaling, photosynthesis and other unknown functions In this study identification of several candidate genes whose promoters could be used for isolation of specific cis elements responsible for the induction and repression of cold regulated genes has been done. Source

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