Kushwaha H.,Integral University |
Gupta S.,Deen Dayal Upadhyay Gorakhpur University |
Rastogi S.,Integral University |
Yadav D.,Deen Dayal Upadhyay Gorakhpur University
Molecular Biology Reports | Year: 2011
The Dof (DNA binding with One Finger) family represents a classic zinc-finger transcription factors involved with multifarious roles exclusively in plants. There exists great diversity in terms of number of Dof genes observed in different crops. In current study, a total of 28 putative Dof genes have been predicted in silico from the recently available whole genome shotgun sequence of Sorghum bicolor (L.) Moench (with assigned accession numbers TPA:BK006983-BK007006 and TPA:BK007079-BK007082). The predicted SbDof genes are distributed on nine out of ten chromosomes of sorghum and most of these genes lack introns based on canonical intron/exon structure. Phylogenetic analysis of 28 SbDof proteins resulted in four subgroups constituting six clusters. The comparative phylogenetic analysis of these Dof proteins along with 30 rice and 36 Arabidopsis Dof proteins revealed six major groups similar to what has been observed earlier for rice and Arabidopsis. Motif analysis revealed the presence of conserved 50-52 amino acids Dof domain uniformly distributed across all the 28 Dof proteins of sorghum. The in silico cis-regulatory elements analysis of these SbDof genes suggested its diverse functions associated with light responsiveness, endosperm specific gene expression, hormone responsiveness, meristem specific expression and stress responsiveness. © 2011 Springer Science+Business Media B.V.
Singh R.S.,Deen Dayal Upadhyay Gorakhpur University |
Prakash H.,Allahabad University
Annals of Physics | Year: 2013
Polarization of light signifies transversal, anisotropic and asymmetrical statistical properties of electromagnetic radiation about the direction of propagation. Traditionally, optical-polarization is characterized by Stokes' theory susceptible to be insufficient in assessing the polarization structure of optical quantum fields and, also, does not decipher the twin characteristic polarization parameters ('ratio of real amplitudes and difference in phases'). An alternative way, in the spirit of classical description of optical-polarization, is introduced which can be generalized to deal higher-order polarization of quantum light, particularly, prepared in non-Gaussian Schrodinger Cat or Cat-like states and entangled bi-modal coherent states. On account of pseudo mono-modal or multi-modal nature of such optical quantum field, higher-order polarization is seen to be highly sensitive to the basis of description. © 2013 Elsevier Inc.
Shivam K.,Deen Dayal Upadhyay Gorakhpur University |
Mishra S.K.,Deen Dayal Upadhyay Gorakhpur University
Process Biochemistry | Year: 2010
An extracellular thermostable α-galactosidase from Aspergillus parasiticus MTCC-2796 was purified 16.59-fold by precipitation with acetone, followed by sequential column chromatography with DEAE-Sephadex A-50 and Sephadex G-100. The purified enzyme was homogeneous on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). It was found to be a monomeric protein with a molecular weight of about 67.5kDa. The purified enzyme showed optimum activity against o-nitrophenyl-α-d-galactopyranoside (oNPG) at pH 5.0 and a temperature of 50°C. The enzyme was thermostable, showing complete activity even after heating at 65°C for 30min. The enzyme showed strict substrate specificity for α-galactosides and hydrolyzed oNPG (Km=0.83mM), melibiose (Km=2.48mM) and raffinose (Km=5.83mM). Among metal ions and reagents tested, Ca2+ and K+ enhanced the enzymatic activity, but Mg2+, Mn2+, ethylenediaminetetraacetic acid (EDTA) and 2-mercaptoethanol showed no effect, while Ag+, Hg2+ and Co2+ strongly inhibited the activity of the enzyme. The enzyme catalyzed the transglycosylation reaction for the synthesis of melibiose. © 2010 Elsevier Ltd.
Kant Upadhyay R.,Deen Dayal Upadhyay Gorakhpur University
BioMed Research International | Year: 2013
JE is a flavivirus generated dreadful CNS disease which causes high mortality in various pediatric groups. JE disease is currently diagnosed by measuring the level of viral antigens and virus neutralization IgM antibodies in blood serum and CSF by ELISA. However, it is not possible to measure various disease-identifying molecules, structural and molecular changes occurred in tissues, and cells by using such routine methods. However, few important biomarkers such as cerebrospinal fluid, plasma, neuro-imaging, brain mapping, immunotyping, expression of nonstructural viral proteins, systematic mRNA profiling, DNA and protein microarrays, active caspase-3 activity, reactive oxygen species and reactive nitrogen species, levels of stress-associated signaling molecules, and proinflammatory cytokines could be used to confirm the disease at an earlier stage. These biomarkers may also help to diagnose mutant based environment specific alterations in JEV genotypes causing high pathogenesis and have immense future applications in diagnostics. There is an utmost need for the development of new more authentic, appropriate, and reliable physiological, immunological, biochemical, biophysical, molecular, and therapeutic biomarkers to confirm the disease well in time to start the clinical aid to the patients. Hence, the present review aims to discuss new emerging biomarkers that could facilitate more authentic and fast diagnosis of JE disease and its related disorders in the future. © 2013 Ravi Kant Upadhyay.
Singh H.J.,Deen Dayal Upadhyay Gorakhpur University |
Upadhyay M.K.,Deen Dayal Upadhyay Gorakhpur University
Journal of Energetic Materials | Year: 2013
Structure optimization and frequency calculation of six nitro derivatives of 1,3,5-triazepine were performed using a MP2(FULL)/6-311G(d,p) method. In order to obtain reliable energy data, single-point energy and subsequently thermodynamic properties of the species considered were calculated at a fairly high level of theory, CCSD(T)/6-311G(d,p). Solid-phase heats of formation and crystal density were determined using an electrostatic potential (ESP) method utilizing wave function analysis-surface analysis suite (WFA-SAS) code. The result shows that all nitro derivatives possess high positive heats of formation that increase with an increase in the number of nitro groups attached to the ring moiety. The crystal density was found to be in the range of 1.67-1.90 g/cm3. Detonation properties of the compounds were estimated using the Kamlet-Jacobs equation. The results showed that detonation velocity (D) and detonation pressure (P) increased with an increase in the number of nitro groups attached at the ring moiety. It was found that all six nitro derivatives of the title compound had better or comparable performance characteristics than the most widely used commercial explosives, such as TNT, research and development explosives (RDX), and 1,3,5,7-tetranitro-1,3,5,7-tetrazocane (HMX). The trinitro derivative (1,3,5-trinitro-1,3,5-triazepine, F) yielded detonation pressure (P) and detonation velocity (D) of 45.5 GPa and 9.23 km/s, respectively, at a loading density of 1.90 g/cm3, which are superior to the most powerful available explosive HMX (P = 39.00 GPa and D = 9.11 km/s). The results obtained during the present study show that the title compounds can be used as promising futuristic high-energy-density materials (HEDMs). © 2013 Copyright Taylor and Francis Group, LLC.
Yadava L.,Deen Dayal Upadhyay Gorakhpur University |
Verma R.,Deen Dayal Upadhyay Gorakhpur University |
Singh R.S.,Deen Dayal Upadhyay Gorakhpur University
Thin Solid Films | Year: 2012
A grided Pd/TiO 2/Si (Pdtisin) gas sensor is fabricated to detect hydrocarbons such as acetone, ethanol and trichloroethylene. The sensitivity measurements are carried out in various ambient (O 2, N 2 and Ar) at room temperature which revealed that fabricated structure attains maximal response for acetone in contrast to other vapors examined. The study of ambient-effect on the device shows that it out-performs in oxygen ambient. A catalytic oxidation mechanism for detection of acetone with a model based upon Langmuir law of adsorption and Frenkel-Poole theory of electronic emission for the description of sensing behavior and vindication of experimental results have been proposed. © 2011 Elsevier B.V. All rights reserved.
Malviya N.,Deen Dayal Upadhyay Gorakhpur University |
Yadav D.,Deen Dayal Upadhyay Gorakhpur University
Genetic Engineering and Biotechnology Journal | Year: 2010
The genetic diversity of 17 cultivars of pigeon pea using 17 random amplified polymorphic DNA (RAPD) markers has been reported. A total of 198 bands were scored corresponding to an average of 11.6 bands per primer with 148 bands showing polymorphism (74.7%). Nine out of eighteen primers gave more than 80% polymorphism. Jaccard similarity coefficient ranged from 0.272 to 0.778. A dendrogram constructed based on the UPGMA clustering method revealed two major clusters. Cluster-I comprises of 12 cultivars which was further differentiated into two sub-clusters having six cultivars each. The cluster-II includes remaining five cultivars. The cultivar IPA-3088 was quite unique from the remaining cultivars as evident in the dendrogram.
Srivastava P.,Deen Dayal Upadhyay Gorakhpur University |
Singh H.J.,Deen Dayal Upadhyay Gorakhpur University
Journal of Energetic Materials | Year: 2010
Nitrogen heterocycles and their derivatives are one of the classes of compounds that have been widely used in developing high-energetic materials. The energy content of the heterocycle ring systems can be increased by inclusion of nitro, cyano, and azido groups. In the present study nitro derivatives of small three-membered B-N-C ring compounds, viz. 1-nitro-1,2-azaboriridine, 1,3-dinitro-1,2-azaboriridine, and 1,3,3-trinitro-1,2-azaboriridine, have been considered. Thermodynamic properties and energetics of the above compounds have been taken for detailed computational study using G2, G3, and CBSQB3 compound methods. Studies reveal that these compounds can be considered for use as high-energetic materials. Detonation velocity (D) and detonation pressure (P) of the title compounds have also been evaluated using the Kamlet-Jacobs method based on the theoretical densities and heats of formation calculated at G3 level. Calculation shows that 1,3,3-trinitro-1,2-azaboriridine yields a detonation velocity of 9.14 km/s and a detonation pressure of 40.2 GPa at a loading density of 1.91 g/cm3 that is comparable to powerful commercial explosives such as HMX (9.10 km/s, 1.91 g/cm3, 39.0 GPa) and RDX (8.75 km/s, 1.82 g/cm3, 34.0 GPa). © Taylor & Francis Group, LLC.
Thakur S.K.,Deen Dayal Upadhyay Gorakhpur University
Molecular Crystals and Liquid Crystals | Year: 2014
A comparative analysis of some mesogenic and nonmesogenic molecules of 2, 5 di-substituted pyridine derivatives molecules has been carried out. The multicenter multipole expansion method is used to obtain the various interaction energy terms. The phase morphology of the mesogenic molecular system has been further investigated with the aid of various energy terms of configurations. The multicenter multipole expansion method is satisfactory and predicts the mesogenic behavior of molecular systems. © 2014 Taylor and Francis Group, LLC.
Yadava L.,Deen Dayal Upadhyay Gorakhpur University |
Verma R.,Deen Dayal Upadhyay Gorakhpur University
Sensors and Actuators, B: Chemical | Year: 2010
The tin oxide (SnO2) thick film gas sensor is fabricated by employing screen-printing technology. This pure SnO2 thick film is doped with 1 or 2 wt% of cadmium sulphide (CdS) by its weight and, thereby, the effect of dopant is presented. X-ray diffraction (XRD) analyses are administrated, which suggest that CdS dopant inhibits the crystallite growth leading to nanometric reduction in grain size. The fabricated gas sensor is responsively studied on exposure to liquid petroleum gas (LPG), methanol, and acetone. It is observed that CdS (2 wt%) doped structure exhibited highest response and is more selective to methanol (70 for 5000 ppm) over LPG and acetone at the operating temperature 200 °C. The CdS-doping improved response- and recovery-time from 90 s and 200 s, for undoped-film, to 40 s and 110 s for methanol (5000 ppm at 200 °C). © 2009 Elsevier B.V. All rights reserved.