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Liang D.,Harbin Medical University | Qin Y.,Harbin Medical University | Zhao W.,Harbin Medical University | Zhai X.,Harbin Medical University | And 9 more authors.
Cancer Letters | Year: 2011

S-allylmercaptocysteine (SAMC), one of the water-soluble organosulfur garlic derivatives, has been demonstrated as a suppressive agent against some tumors. The effects of SAMC on the proliferation and metastasis of colorectal cancer (CRC) under in vitro and in vivo conditions were evaluated here. The viabilities and migrations of CRC cells SW480, SW620, Caco-2 treated with SAMC were measured by MTT, scratch-wound, and transwell assays. The in vivo anticancer effect of SAMC against luciferase-expressing SW620 xenografts in mice was determined by bioluminescence imaging and histopathology observation. The apoptosis of SAMC-treated CRC cells was examined by Western blotting. The results demonstrate that SAMC could effectively suppress the growth and metastasis of colorectal cancer cells both in vivo and in vitro. The anticancer effect of SAMC was related to the decreased proliferation and increased apoptosis as well as necrosis of cancer cells. Oral administration of SAMC in the quantity/concentration used had no apparent toxic side effect on the vital organs of the experimental mice. Taken together, the proliferation and metastasis of CRC cells can be significantly suppressed by SAMC treatment under both in vitro and in vivo conditions. SAMC may thus be a promising candidate for CRC chemotherapy. © 2011 Elsevier Ireland Ltd. Source


Li Y.,South China University of Technology | Li Y.,Xiangtan University | Liao X.,South China University of Technology | Chen G.,South China University of Technology | And 2 more authors.
Molecular Biotechnology | Year: 2011

Microcystis viridis lectin (MVL), a sugar-binding protein originally isolated from freshwater blue-green algae Microcystis viridis, has been reported to have potent anti-HIV activity. In this paper, we described the expression and purification of recombinant-MVL (R-MVL) gene in E. coli. The results demonstrated that the R-MVL in shake flask cultures was primarily expressed either in the form of inclusion bodies at 37°C or in the soluble fraction at 23 °C. Secondly, a one-step purification based on nickel-affinity chromatography was employed and 15 mg of highly purified (>95%) R-MVL from 1 l of cell cultures was yielded. The purified R-MVL was then subjected to MALDI-TOF-MS analysis for protein identification. In conclusion, for the first time, the R-MVL was successfully cloned and expressed in E. coli, which is useful for further study and large-scale cost-effective production of MVL protein. © 2010 Springer Science+Business Media, LLC. Source


Manu K.A.,National University of Singapore | Shanmugam M.K.,National University of Singapore | Ramachandran L.,National University of Singapore | Li F.,National University of Singapore | And 6 more authors.
Clinical Cancer Research | Year: 2012

Purpose: Because of poor prognosis and development of resistance against chemotherapeutic drugs, the existing treatment modalities for gastric cancer are ineffective. Hence, novel agents that are safe and effective are urgently needed. Whether γ-tocotrienol can sensitize gastric cancer to capecitabine in vitro and in a xenograft mouse model was investigated. Experimental Design: The effect of γ-tocotrienol on proliferation of gastric cancer cell lines was examined by mitochondrial dye uptake assay, apoptosis by esterase staining, NF-κB activation by DNA-binding assay, and gene expression by Western blotting. The effect of γ-tocotrienol on the growth and chemosensitization was also examined in subcutaneously implanted tumors in nude mice. Results: γ-Tocotrienol inhibited the proliferation of various gastric cancer cell lines, potentiated the apoptotic effects of capecitabine, inhibited the constitutive activation of NF-κB, and suppressed the NF-κB- regulated expression of COX-2, cyclin D1, Bcl-2, CXCR4, VEGF, and matrix metalloproteinase-9 (MMP-9). In a xenograft model of human gastric cancer in nude mice, we found that administration of γ-to-cotrienol alone (1 mg/kg body weight, intraperitoneally 3 times/wk) significantly suppressed the growth of the tumor and this effect was further enhanced by capecitabine. Both the markers of proliferation index Ki-67 and for microvessel density CD31 were downregulated in tumor tissue by the combination of capecitabine and γ-tocotrienol. As compared with vehicle control, γ-tocotrienol also suppressed the NF-κB activation and the expression of cyclin D1, COX-2, intercellular adhesion molecule-1 (ICAM-1), MMP-9, survivin, Bcl-xL, and XIAP. Conclusions: Overall our results show that γ-tocotrienol can potentiate the effects of capecitabine through suppression of NF-κB-regulated markers of proliferation, invasion, angiogenesis, and metastasis. ©2012 AACR. Source


Siveen K.S.,National University of Singapore | Ahn K.S.,Kyung Hee University | Ong T.H.,Humphrey Oei Institute of Cancer Research | Shanmugam M.K.,National University of Singapore | And 14 more authors.
Oncotarget | Year: 2014

Angiogenesis is one of the key hallmarks of cancer. In this study, we investigated whether γ-tocotrienol can abrogate angiogenesis-mediated tumor growth in hepatocellular carcinoma (HCC) and if so, through what molecular mechanisms. We observed that γ-tocotrienol inhibited vascular endothelial growth factor (VEGF)-induced migration, invasion, tube formation and viability of HUVECs in vitro. Moreover, γ-tocotrienol reduced the number of capillary sprouts from matrigel embedded rat thoracic aortic ring in a dose-dependent manner. Also, in chick chorioallantoic membrane assay, γ-tocotrienol significantly reduced the blood vessels formation. We further noticed that γ-tocotrienol blocked angiogenesis in an in vivo matrigel plug assay. Furthermore, γ-tocotrienol inhibited VEGF-induced autophosphorylation of VEGFR2 in HUVECs and also suppressed the constitutive activation of AKT/mammalian target of rapamycin (mTOR) signal transduction cascades in HUVECs as well as in HCC cells. Interestingly, γ-tocotrienol was also found to significantly reduce the tumor growth in an orthotopic HCC mouse model and inhibit tumor-induced angiogenesis in HCC patient xenografts through the suppression of various biomarkers of proliferation and angiogenesis. Taken together, our findings strongly suggest that γ-tocotrienol might be a promising anti-angiogenic drug with significant antitumor activity in HCC. Source


Trademark
Davos Life Science Pte Ltd | Date: 2012-05-08

Anti-oxidants for use in the manufacture of cosmetics, skin care products, hair care products, dental care products, personal care products, perfumery, vitamins, pharmaceuticals, beverages, food products, and food supplements; chemical additives and chemical preparations for use in the manufacture of beauty care, cosmetics, dental care, facial care, hair care, hair styling, perfumery, personal care, personal hygiene, skin care, sun blocking, sun screening, sun tanning products as well as beverages, vitamins and pharmaceuticals; biological preparations for use in the manufacture of cosmetics, other than for medical or veterinary use; all of the aforesaid goods containing tocotrienols in whole or significant part. Perfumes; sun blocking preparations; sun screening preparations; sun tanning preparations; preparations for beauty care, namely, beauty care cosmetics; preparations for dental care, namely, non-medicated dental rinse, toothpaste; preparations for facial care, namely, face lotions, face creams; hair care preparations; hair styling preparations; preparations for personal care, namely, deodorants for personal use, fragrances for personal use, soaps for personal use; preparations for personal hygiene, namely, personal deodorants; non-medicated skin care preparations; cosmetic oils, cosmetic creams; essential oils; natural essential oils; cosmetic preparations for use in beauty care, dental care, facial care, hair care, hair styling, perfume, personal care, personal hygiene, skin care, sun blocking, sun screening and sun tanning, namely, beauty creams, toothpastes, skin creams, hair creams, perfumes, cosmetic sunscreen preparations; all of the aforesaid goods containing tocotrienols in whole or significant part. Beverages adapted for medical and medicinal purposes, namely, dietetic beverages, nutritionally-fortified beverages; chemicals, chemical preparations, and chemical products for medical and pharmaceutical purposes, namely, chemical reagents; food supplements; medicated supplements for foodstuffs for human consumption; dietetic foods adapted for medical purposes; health food supplements made principally of minerals, vitamins; health food supplements for persons with special dietary requirements; medicated supplements for food for human consumption; medicated creams and preparations for skin care, hair care, facial care, personal care, personal hygiene, and protecting the skin against sun damage; products for dental care, namely, medicated mouth care and treatment preparations; mineral supplements; medicinal natural oils; natural pharmaceutical products, namely, pharmaceuticals for the prevention and treatment of cancer, for treating diabetes, for treating skin disorders; non-prescription dietary supplements for human consumption consisting of vitamins, mineral supplements and anti-oxidants; nutritional supplements; medicinal oils for medical purposes and pharmaceutical purposes; medicated preparations for use as nutritional additives to food for human consumption; pharmaceutical compositions, drugs, preparations, products and substances, namely, pharmaceuticals for the prevention and treatment of cancer, for treating diabetes, for treating skin disorders; vitamin supplements; all of the aforesaid goods containing tocotrienols in whole or significant part. Fruit based and dairy based dietary supplements, other than for medical use, namely, fruit-based snack food, fruit-based spreads, milk-based beverages containing fruit juice, beverages having a milk base; protein-based dairy preparations, namely, milk proteins, dairy-based beverages, dairy-based spreads, protein based, nutrient-dense snack foods; fruit based and dairy based food preparations being dietetic supplements or nutritional additives, not for medical purposes, namely, fruit-based snack food, fruit-based spreads, milk-based beverages containing fruit juice, beverages having a milk base; all of the aforesaid goods containing tocotrienols in whole or significant part. Dental health chewing gum, other than medicated; carbohydrate-based food preparations being dietetic supplements or nutritional additives, not for medical purposes, namely, processed cereals, wheat-based snack foods; dietary supplements, other than for medical use, namely, food flavorings, food seasonings, flavoring additives for non-nutritional purposes, wheat germ; all of the aforesaid goods containing tocotrienols in whole or significant part. Beverages containing added vitamins, minerals, anti-oxidants, proteins, other than for medical use, namely, fruit beverages, water beverages, vegetable juice beverages, aloe juice beverages, fruit-flavored beverages, isotonic beverages, fruit-based beverages, whey beverages; all of the aforesaid goods containing tocotrienols in whole or significant part.

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