Daqing Normal College

Daqing, China

Daqing Normal College

Daqing, China
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Shi D.,China Agricultural University | Shi D.,Daqing Normal College | Li L.,China Agricultural University | Zhang J.,China Agricultural University | And 5 more authors.
African Journal of Biotechnology | Year: 2011

Chlorophyll (Chl) is the key pigment involved in photosynthesis. Analysis of the expression pattern of Chl metabolic genes will contribute to our understanding of photosynthesis. Also, the genes coding for Chl metabolism are ideal targets for revealing the evolution relationships of photosynthetic organisms. In this study, we summarized the Chl metabolic pathway in higher plants and conducted in silico expression analysis of related genes in maize. Phylogenetic analysis revealed that the evolution of Chl metabolic genes proceeded in a certain direction. Moreover, the diversity of some rate-limiting enzymes might have played a positive role in the evolution of Chl metabolism. © 2011 Academic Journals.


Zheng W.-H.,Wenzhou University | Zheng W.-H.,Key Laboratory for Water Environment and Marine Biological Resources Protection in Zhejiang Province | Li M.,Daqing Normal College | Liu J.-S.,Wenzhou University | And 3 more authors.
Physiological and Biochemical Zoology | Year: 2014

Phenotypic flexibility of various morphological and physiological characters is widespread in animals. Resident endothermic animals of temperate climates provide a natural experiment in phenotypic flexibility. In this study, we took an integrative approach to assess seasonal and geographic influences on metabolism in Eurasian tree sparrows (Passer montanus). We measured resting metabolic rate (RMR), masses of internal organs, mitochondrial respiration capacities in liver and muscle, cytochrome C oxidase (COX) activities in liver and muscle, and circulating levels of plasma triiodothyronine (T3) and thyroxine (T4) in summer and winter sparrows at two sites from southeastern (Wenzhou) and northeastern (Qiqihar) China that differ in climate. Body masses of tree sparrows were significantly higher in winter than in summer at both sites but did not differ with latitude. RMRs of tree sparrows varied significantly with both latitude and season, with RMRs of Qiqihar birds being higher than those of Wenzhou birds and with RMRs being higher in winter than in summer. Consistently, dry masses of brain, lung, liver, gizzard, small intestine, rectum, and total digestive tract varied significantly with either latitude or season. State 4 respiration and COX activity in liver and muscle were remarkably higher in Qiqihar and increased significantly in winter. Circulating levels of plasma T3 also showed significant seasonal and latitudinal variation and was higher in Qiqihar in winter than in other groups. These data suggest that tree sparrows mainly coped with cold by enhancing thermogenic capacities through heightened activity of respiratory enzymes and higher levels of plasma thyroid hormones (T3). These results are consistent with a pronounced seasonal and latitudinal phenotypic flexibility mediated through physiological and biochemical adjustments in Eurasian tree sparrows. © 2014 by The University of Chicago. All rights reserved.


Wang S.-X.,Nankai University | Yuan G.-X.,Nankai University | Yuan G.-X.,Daqing Normal College | Kendrick R.C.,Kadoorie Farm and Botanic Garden
Zootaxa | Year: 2011

Three new species of the genus Variacma Wang are described from Hong Kong, China: V. hongkongensis sp. nov., V. bifariafera sp. nov. and V. tomentosa sp. nov. The female is recorded for the first time for the genus and the generic characters are redescribed. Images of adults and genitalia are provided, along with a key to all the known species based on the male genitalia. Copyright © 2011 Magnolia Press.


Zheng W.-H.,Wenzhou University | Zheng W.-H.,Zhejiang Provincial Key Laboratory for Subtropical Water Environment | Lin L.,Wenzhou University | Liu J.-S.,Wenzhou University | And 3 more authors.
Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology | Year: 2013

Acclimatization to different ambient conditions is an essential prerequisite for survival of small passerine birds. Long-distance migration and winter acclimatization induce similar physiological and biochemical adjustments in passerines. To understand metabolic adaptations, the resting metabolic rate (RMR), the thermogenic properties of mitochondria in liver and muscle, and the activity of thyroid hormones were examined in field-captured little buntings (Emberiza pusilla) between Southeastern (Wenzhou) and Northeastern (Qiqihar) China from March to May in 2008 during their migration. Twelve birds were trapped from March to April in Wenzhou region, Zhejiang Province (27°29'N, 120°51'E) and eleven birds originated from April to May in Qiqihar region, Heilongjiang Province (47°29'N, 124°02'E). We found that RMRs of little buntings were significantly higher in Qiqihar than in Wenzhou. Consistently, mitochondrial state-4 respiration capacities and cytochrome c oxidase activities (COX) in liver and muscle, and circulating levels of plasma triiodothyronine (T3) of little buntings were also significantly higher in Qiqihar than in Wenzhou. Variation in metabolic biochemical markers of liver and muscle, such as state-4 respiration and COX, and variation in thyroid hormone levels were correlated with variation in RMR. There was also a positive relationship between T3 and metabolic biochemical markers. Little buntings mainly coped with a cold environment by enhancing thermogenic capacities through enhanced respiratory enzyme activities and plasma T3. These results support the view that the primary means by which small birds meet energetic challenges of cold conditions is through metabolic adjustments. © 2012 Elsevier Inc.


Luan B.-F.,Chongqing University | Pei Y.-F.,Daqing Normal College | Huang T.-L.,Chongqing University | Ye Q.,Chongqing University | And 2 more authors.
Chongqing Daxue Xuebao/Journal of Chongqing University | Year: 2010

Aimed at electronic packaging application, the different types of particles of Al2O3, AlN and SiC which the mean size is about 4 μ m are selected and three types of composites are fabricated by squeeze casting method with the particle volume fraction is 40%. The results show that the microstructure of composites is dense and free of pores with the particle distribute uniformly. The mean linear coefficient of thermal expansion (CTE) of composites ranges from 11.51 × 10-6~18.62 × 10-6/K and increases with increasing temperature. The measured coefficient of thermal expansion of SiCp/2024 is lower than that of AlNp/6061 and Al2O3p/2024. The measured CTE values of Al2O3p/2024 composites agreed well with Kerner's model, the CTE of AlNp/6061 is closer to the prediction by ROM, and the CTE of SiCp/2024 varies from lower limit to upper limit of Shapery's model.


Li S.,Northeast Agricultural University | Li M.,Northeast Agricultural University | Li M.,Daqing Normal College | Cui Y.,Northeast Agricultural University | Wang X.,Northeast Agricultural University
Pesticide Biochemistry and Physiology | Year: 2013

The effect of avermectin was studied on King pigeon brain nerve cells by cytotoxicity [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide, MTT] and apoptosis [acridine orange/ethidium bromide (AO/EB) assay, transmission electron microscope (TEM) evaluation, measurement of mitochondrial membrane potential (δψm), phosphatidylserine (PS) exposure, caspases activities, DNA fragmentation, reactive oxygen species (ROS) and caspase-3 mRNA expression] within the 2.5-10μgL-1 concentration-range. The results revealed that within the concentrations of 2.5-10μgL-1, avermectin showed obvious cytotoxicity and induced apoptosis in a dose-dependent manner to neurons of King pigeon in vitro. Cell viability were 99.93±8.52%, 82.02±4.99% and 78.23±5.67% after 24h of treatment with avermectin at the concentrations of 0, 2.5 and 5μgL-1, which decreased to 56.36±2.17% of 10μgL-1. Treated cells showed typical apoptosis morphological changes including cytoplasmic vacuolation, chromatin condensation, unclear nuclear membrane and decreased/swollen mitochondria. Typical biochemical hallmarks of apoptosis including δψm loss, PS exposure, activations of caspase-3, caspase-8 and caspase-9, DNA fragmentation were observed too. Moreover, the levels of ROS in the avermectin treatment groups increased significantly compared to control group. Furthermore, the caspase-3 mRNA levels increased significantly following AVM treatment. In conclusion, our experimental results show that avermectin has cytotoxicity to brain neurons of King pigeon in vitro and the mechanism of neurotoxicity induced by avermectin is closely related to apoptosis. © 2013 Elsevier Inc.


Liu S.,Northeast Agricultural University | Xu F.-P.,Northeast Agricultural University | Yang Z.-J.,Northeast Agricultural University | Li M.,Northeast Agricultural University | And 3 more authors.
Biological Trace Element Research | Year: 2014

Cadmium (Cd) is an important environmental pollutant present in soil, water, air, and food. Selenium (Se) can antagonize some metal element toxicity including Cd. To investigate the cytotoxicity of Cd and the protective effects of Se on bird immunocytes in vitro, chicken splenic lymphocytes with CdCl 2 (10-6 mol/L), Na2SeO3 (10 -7 mol/L), and the mixture (10-7 mol/L Na 2SeO3 and 10-6 mol/L CdCI2) were incubated for 12, 24, 36, and 48 h, respectively. A high level of malondialdehyde (MDA) and reactive oxygen species (ROS) productions were observed in Cd treatment group; the activities of catalase (CAT), glutathione peroxidise (GSH-Px), superoxide dismutase (SOD), and the mitochondrial inner transmembrane potential (ΔΨm) were significantly lower in Cd treatment group than those in controls (P<0.05 or P<0.01). In contrast, Se significantly improved the activities of antioxidant enzymes and reduced MDA and ROS levels compared to Cd treatment alone group, although not restored to the levels of control group. The population of apoptosis cells demonstrated that Cd induces the apoptosis of chicken splenic lymphocytes; in addition, increased mRNA level of Bak, p53, caspase-3, caspase-9, and cytochrome c (Cyt c) and decreased Bcl-2, Bcl-xl, and CaM were observed in Cd treatment group. Se ameliorated ΔΨm and [Ca2+]i for mitochondria function restoring, and Se was able to modulate the expression of relative genes. In conclusion, concurrent treatment with Se reduced the Cd-induced morphological changes and oxidative stress, ion disorder, and apoptosis, suggesting that the toxic effects of Cd on the chicken splenic lymphocytes were partly meliorated by Se. © 2014 Springer Science+Business Media.


Qu J.,Northeast Agricultural University | Li M.,Northeast Agricultural University | Li M.,Daqing Normal College | Zhao F.,Northeast Agricultural University | And 4 more authors.
Ecotoxicology and Environmental Safety | Year: 2015

Avermectin (AVM) is used in agriculture and veterinary medicine for the prevention of parasitic diseases; AVM is the active component of some insecticidal and nematicidal products. Residues of AVM drugs or their metabolites in livestock feces have toxic effects on non-target aquatic and terrestrial organisms. In this study, changes in the levels of autophagy related genes and ultrastructure in pigeon brain tissues after subchronic exposure to AVM for 30, 60 and 90 d were investigated. The decrease in the mRNA levels of TORC1 and TORC2 and increase in the mRNA levels of LC3, Beclin 1, Dynein, ATG5 and ATG4B and the increase in the protein levels of LC3, Beclin 1 and Dynein in a dose- and time-dependent manner in the pigeon brain were observed. The number of autophagic vacuoles in the cerebrum, cerebellum and optic lobe increased significantly with the concentration of AVM and the exposure time. We found that the changes in the levels of autophagy related genes and the ultrastructure in the cerebrum were more obvious than in the cerebellum and the optic lobe. The results suggest that AVM could induce autophagy in pigeon brain tissues. The information presented in this study is helpful for understanding the mechanism of AVM-induced autophagy in birds. © 2014 Elsevier Inc.


Li M.,Northeast Agricultural University | Li M.,Daqing Normal College | You T.-Z.,Northeast Agricultural University | Zhu W.-J.,Northeast Agricultural University | And 5 more authors.
Ecotoxicology | Year: 2013

Avermectins (AVMs) are the active components of some insecticidal and nematicidal products used in agriculture and veterinary medicine for the prevention of parasitic diseases. Residues of AVM drugs or their metabolites in livestock feces have toxic effects on non-target aquatic and terrestrial organisms. In this study, oxidative stress responses and pathological changes on pigeon brain tissues and serum after subchronic exposure to AVM for 30, 60 and 90 days were investigated. The decrease in antioxidant enzyme (superoxide dismutase, SOD and glutathione peroxidase, GSH-Px) activities and increase in methane dicarboxylic aldehyde content in a dose-time-dependent manner in the brain and serum of pigeon were observed. The protein carbonyl content, an indicator of protein oxidation, and DNA-protein crosslink coefficient were significantly augmented with dose-time-dependent properties. The microscopic structures of the cerebrum, cerebellum and optic lobe altered obviously, the severity of which increased with the concentration of AVM and exposure time. The results imply that AVM could induce oxidative damage to the brain tissue and serum of pigeon. The information presented in this study is helpful to understand the mechanism of AVM-induced oxidative stress in birds. © 2013 Springer Science+Business Media New York.


You L.,Northeast Agricultural University | Liu C.,Northeast Agricultural University | Yang Z.-J.,Northeast Agricultural University | Li M.,Northeast Agricultural University | And 2 more authors.
Biological Trace Element Research | Year: 2014

Selenoprotein T (SelT) is associated with the regulation of calcium homeostasis and neuroendocrine secretion. SelT can also change cell adhesion and is involved in redox regulation and cell fixation. However, the structure and function of chicken SelT and its response to selenium (Se) remains unclear. In the present study, 150 1-day-old chickens were randomly divided into a low Se group (L group, fed a Se-deficient diet containing 0.020 mg/kg Se) and a control group (C group, fed a diet containing sodium selenite at 0.2 mg/kg Se). The immune organs (spleen, thymus, and bursa of Fabricius) were collected at 15, 25, 35, 45, and 55 days of age. We performed a sequence analysis and predicted the structure and function of SelT. We also investigated the effects of Se deficiency on the expression of SelT, selenophosphate synthetase-1 (SPS1), and selenocysteine synthase (SecS) using RT-PCR and the oxidative stress in the chicken immune organs. The data showed that the coding sequence (CDS) and deduced amino acid sequence of SelT were highly similar to those of 17 other animals. Se deficiency induced lower (P<0.05) levels of SelT, SPS1, and SecS, reduced the catalase (CAT) activity, and increased the levels of hydrogen peroxide (H2O2) and hydroxyl radical (-OH) in immune organs. In conclusion, the CDS and deduced amino acid sequence of chicken SelT are highly homologous to those of various mammals. The redox function and response to the Se deficiency of chicken SelT may be conserved. A Se-deficient diet led to a decrease in SelT, SecS, and SPS1 and induced oxidative stress in the chicken immune organs. To our knowledge, this is the first report of predictions of chicken SelT structure and function. The present study demonstrated the relationship between the selenoprotein synthases (SPS1, SecS) and SelT expression in the chicken immune organs and further confirmed oxidative stress caused by Se deficiency. Thus, the information presented in this study is helpful to understand chicken SelT structure and function. Meanwhile, the present research also confirmed the negative effects of Se deficiency on chicken immune organs. © 2014 Springer Science+Business Media.

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