Daqing Medical College

Daqing, China

Daqing Medical College

Daqing, China
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Zhang B.,Harbin Medical University | Dong J.-L.,Daqing Oilfield General Hospital | Chen Y.-L.,Harbin Medical University | Liu Y.,Harbin Medical University | And 4 more authors.
Molecular Medicine Reports | Year: 2017

Homocysteine (Hcy) and glutathione (GSH) are crucial reduction-oxidation mediators. The underlying mechanisms governing the effects of Hcy on GSH generation in the progression of alcoholic liver disease has so far received little attention. The present study hypothesized that the antioxidant transcriptional factor nuclear factor (erythroid-derived 2)-like 2 (Nrf2) may participate in Hcy-mediated regulation of GSH production in HepG2 human liver cancer cells. MTT assay was used to study the cytotoxicity of homocysteine, western blot analysis and immunofluorescence staining were used to determine the effect of Hcy on Nrf2 expression. Our data demonstrated that HepG2 cells exposed to exogenous levels of Hcy (0-100 μM) exhibited elevated GSH levels in a concentration-dependent manner. Furthermore, 4-hydroxynonenal (4-HNE)-induced cell injury was attenuated by Hcy; however, this protective effect was blocked by the GSH-production inhibitor buthionine sulfoximine. Hcy treatment was able to induce Nrf2 protein expression in HepG2 cells. Treatment with the Nrf2 activator tert-butylhydroquinone (0-100 μM) increased GSH expression in a concentration-dependent manner; however, Nrf2-siRNA abolished the Hcy-induced increase in GSH expression and cellular protection in 4-HNE-stressed HepG2 cells. In conclusion, the antioxidant transcriptional factor Nrf2 was demonstrated to mediate the Hcy-induced increase in GSH expression levels and cellular protection in HepG2 cells.


Zhang L.-L.,Harbin Medical University | Sun L.,Beijing Hospital and Beijing Institute of Geriatrics | Zhu X.-Q.,Beijing Hospital and Beijing Institute of Geriatrics | Xu Y.,Tianjin Medical University | And 13 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2014

Aims: Genome-wide association studies (GWAS) have identified several risk variants for prostate cancer (pCa) mainly in Europeans, which need to be further verified in other racial groups. We selected six previously identified variants as candidates and to define the association with PCa in Northern Han Chinese. Methods: 749 subjects from Beijing and Tianjin in Northern China were included. Six variants (rs10505474, rs7837328, rs4242384, rs7813, rs486907 and rs1058205) were genotyped by high resolution melting (HRM) assays. The individual and cumulative contribution for of the risk of PCa and clinical covariates were analyzed. Results: Among the six candidate variants, onlyrs10505474, and rs7837328, both locating at 8q24 region, were associated with PCa in our population.rs10505474 (A) was associated with PCa (ORrecessive= 1.56, p=0.006); and rs7837328 (A) was associated with PCa (ORdominant= 1.38, p=0.042/ORrecessive=1.99, p=0.003). Moreover, we observed a cumulative effects between them (ptrend=2.58×10-5). The joint population attributable risk showed the two variants might account for 71.85% of PCa risk. In addition, we found the homozygotes of rs10505474 (A) and rs7837328 (A) were associated with PCa clinical covariants (age at onset, tumor stage, respectively) (ORage=0.046, ORtumorstage =0.048). Conclusion: rs10505474 (A) and rs7387328 (A) at 8q24 are associated with PCa and cumulatively confer risk, suggesting the two variations could determine susceptibility to PCa in the Northern Chinese Han population.


Liu X.,Northeast Forestry University | Liu X.,Heilongjiang Bayi Agricultural University | Jia J.,Daqing Medical College | Yang L.,Northeast Forestry University | And 5 more authors.
Molecules | Year: 2012

The aim of this study was to investigate the antioxidant and free-radical scavenging activity of extract and fractions from various parts of Elsholtzia ciliata. The inflorescences, leaves, stems and roots of E. ciliata were extracted separately and two phenolic component enrichment methods: ethyl acetate-water liquid-liquid extraction and macroporous resin adsorption-desorption, were adopted in this study. The antioxidant activities of water extracts and fractions of E. ciliata were examined using different assay model systems in vitro. The fraction root E (purified by HPD300 macroporous resin) exhibited the highest total phenolics content (497.2 ± 24.9 mg GAE/g), accompanied with the highest antioxidant activity against various antioxidant systems in vitro compared to other fractions. On the basis of the results obtained, E. ciliata extracts can be used potentially as a ready accessible and valuable bioactive source of natural antioxidants. © 2012 by the Authors.


Gao Y.T.,Northeast Petroleum University | Wang L.H.,China National Petroleum Corporation | Miao X.J.,Daqing Medical College | Leng J.C.,Northeast Petroleum University
Advanced Materials Research | Year: 2014

In order to reduce noise disturbance of alternating current field measurement(ACFM) signals in crack defects detection, a new noise cancellation method called empirical mode decomposition(EMD) was proposed. A butt welded plate specimen was inspected and measured by a hand-held ACFM probe and a TSC AMIGO instrument. The original ACFM signal was first decomposed into different intrinsic mode functions(IMFs) and a residue, and some IMFs containing useful information were subsequently reconstructed based on correlation coefficients. The experimental results show that the reconstructed ACFM signals Bx and Bz, and butterfly plot can characterize crack defects more accurately, demonstrating the feasibility and effectiveness of the proposed method. © (2014) Trans Tech Publications, Switzerland.


Liu Z.,Northeast Forestry University | Jia J.,Daqing Medical College | Chen F.,Northeast Forestry University | Yang F.,Northeast Forestry University | And 2 more authors.
Molecules | Year: 2014

An ionic liquid-based microwave-assisted extraction method (ILMAE) was successfully applied for the extraction of taxifolin from Larix gmelinii. Different kinds of 1-alkyl-3-methylimidazolium ionic liquids with different kinds of cations and anions were studied and 1-butyl-3-methylimidazolium bromide was chosen as the optimal solvent for taxifolin extraction. The optimal conditions of ILMAE were determined by single factor experiments and Box-Behnken design as follows: [C4mim]Br concentration of 1.00 M, soaking time of 2 h, liquid-solid ratio of 15:1 mL/g, microwave irradiation power of 406 W, microwave irradiation time of 14 min. No degradation of taxifolin had been observed under the optimum conditions as evidenced from the stability studies performed with standard taxifolin. Compared with traditional solvent and methods, ILMAE provided higher extraction yield, lower energy and time consumption. The distribution of taxifolin in different parts of larch and the influences of age, orientation, and season on the accumulation of taxifolin were analyzed for the sufficient utilization of L. gmelinii. © 2014 by the authors; licensee MDPI, Basel, Switzerland.


Gao Y.T.,Northeast Petroleum University | Wang L.H.,China National Petroleum Corporation | Miao X.J.,Daqing Medical College | Leng J.C.,Northeast Petroleum University
Advanced Materials Research | Year: 2014

Nondestructive inspection and evaluation of welds is very important to ensure the safety of industrial products. In this study, the metal magnetic memory(MMM) and alternating current field measurement(ACFM) methods are utilized to characterize welding defects of a butt welded tubular specimen respectively. The results show that the MMM method has potentials in evaluating both early stress concentration and macro-defects but only qualitatively, while the ACFM method can provide crack depth sizing information quantitatively, suggesting that the two methods should be combined for nondestructive evaluation welding defects to improve the detection efficiency and accuracy. © (2014) Trans Tech Publications, Switzerland.


Zhao Y.,Daqing Oilfield General Hospital | Lv W.,Daqing Medical College | Piao H.,Daqing Oilfield General Hospital | Chu X.,Daqing Oilfield General Hospital | Wang H.,Daqing Oilfield General Hospital
Journal of Receptors and Signal Transduction | Year: 2014

Pulmonary arterial hypertension (PAH) is a vascular remodeling disease characterized by enhanced proliferation of pulmonary artery smooth muscle cells (PASMCs) and suppressed apoptosis. Platelet-derived growth factor (PDGF) is a potent mitogen involved in cell proliferation and migration. PDGF-BB induces the proliferation and migration of PASMCs and has been proposed to be a key mediator in the progression of PAH. Previous studies have shown that PDGF and its receptor are substantially elevated in lung tissues and PASMCs isolated from patients and animals with PAH, but the underlying mechanisms are still poorly manifested. MAP kinases, including extracellular signal-regulated kinase1/2 (ERK1/2), c-Jun NH2-terminal kinase1/2 (JNK1/2), and p38 are the key intracellular signals for stimuli-induced cell proliferation, survival, and apoptosis. Therefore, the purpose of this study is to determine whether PDGF-BB on cell proliferation process is mediated through the MAP kinases pathway in human PASMCs (HPASMCs). Our results showed PDGF-BB-induced proliferating cell nuclear antigen (PCNA), Cyclin A and Cyclin E expression in a concentration-dependent manner. The expression levels of phosphorylated JNK (p-JNK) was upregulated with 20ng/ml PDGF-BB treatment, while PDGF-BB could not increase phosphorylated ERK1/2 (p-ERK1/2) and p-38 (p-p38) expression. The effects of PDGF-BB on cell proliferation and survival were weakened after the administration of antagonist of the JNK pathway or si-JNK. In addition, PDGF-BB protected against the loss of mitochondrial membrane potentials evoked by serum deprivation (SD) in a JNK-dependent manner. These results suggest that PDGF-BB promotes HPASMCs proliferation and survival, which is likely to be mediated via the JNK pathway. © 2014 Informa Healthcare USA, Inc.


Piao H.,Daqing Oilfield General Hospital | Chu X.,Daqing Oilfield General Hospital | Lv W.,Daqing Medical College | Zhao Y.,Daqing Oilfield General Hospital
Journal of Physiological Sciences | Year: 2016

Estrogen withdrawal following menopause results in an increase of osteoclasts formation and bone resorption, which is one of the most important mechanisms of postmenopausal osteoporosis. Recently, growing evidence has suggested that receptor-interacting protein 140 was implicated in estrogen-regulated metabolic disease, including fat metabolism and lipid metabolism. However, little is known regarding the role of receptor-interacting protein 140 in the regulation of bone metabolic by estrogen. In the present study, Western blotting disclosed that estrogen brings a significant increasing expression of receptor-interacting protein 140 in osteoclasts, but not in osteoblasts and bone marrow mesenchymal stem cells. Furthermore, analysis of TRAP staining and bone resorption assay showed that depletion of receptor-interacting protein 140 could significantly alleviate the inhibitory effects of estrogen on osteoclasts formation and bone resorption activity. Moreover, estrogen could induce osteoclasts apoptosis by increasing receptor-interacting protein 140 expression through the Fas/FasL pathway. Taken together, receptor-interacting protein 140 might be a critical player in estrogen-mediated osteoclastogenesis and bone resorption. © 2016 The Physiological Society of Japan and Springer Japan


PubMed | Daqing Oilfield General Hospital and Daqing Medical College
Type: Journal Article | Journal: The journal of physiological sciences : JPS | Year: 2016

Estrogen withdrawal following menopause results in an increase of osteoclasts formation and bone resorption, which is one of the most important mechanisms of postmenopausal osteoporosis. Recently, growing evidence has suggested that receptor-interacting protein 140 was implicated in estrogen-regulated metabolic disease, including fat metabolism and lipid metabolism. However, little is known regarding the role of receptor-interacting protein 140 in the regulation of bone metabolic by estrogen. In the present study, Western blotting disclosed that estrogen brings a significant increasing expression of receptor-interacting protein 140 in osteoclasts, but not in osteoblasts and bone marrow mesenchymal stem cells. Furthermore, analysis of TRAP staining and bone resorption assay showed that depletion of receptor-interacting protein 140 could significantly alleviate the inhibitory effects of estrogen on osteoclasts formation and bone resorption activity. Moreover, estrogen could induce osteoclasts apoptosis by increasing receptor-interacting protein 140 expression through the Fas/FasL pathway. Taken together, receptor-interacting protein 140 might be a critical player in estrogen-mediated osteoclastogenesis and bone resorption.

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