Daqing Branch of HeiLongJiang Academy of science

Daqing, China

Daqing Branch of HeiLongJiang Academy of science

Daqing, China
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Dayo A.Q.,Harbin Engineering University | Dayo A.Q.,University of Balochistan | Gao B.-C.,Daqing Branch of Heilongjiang Academy of science | Wang J.,Harbin Engineering University | And 5 more authors.
Composites Science and Technology | Year: 2017

The short length hemp fibers treated with 5% NaOH (THF) were sandwiched in bisphenol A-aniline based benzoxazine (BA-a) to form the resin-fiber-resin composite. The effects of fluctuating fiber volume on the curing behavior, mechanical, thermomechanical, and thermal properties of the resulted composites were studied through fourier transform infrared (FTIR), differential scanning calorimetry (DSC), dynamic mechanical analyzer (DMA), thermogravimetric analysis (TGA), and scanning electron microscope (SEM). The results confirmed that the rich -OH groups on THF can promote the ring-opening of oxazines at lower curing temperature, and improve the adhesion between fiber and matrix. In addition, an increase of 185% in impact strength, 1.3 GPa in flexural modulus, and 109% in tensile strength was recorded for 25 vol% THF content cured composites. The slight diminution in thermal stability was observed as THF content increased, at 25 vol% THF content, weight loss temperature (T5%) and char yield at 800 °C (Yc) were 11 °C, and 0.4% lower than pristine poly(BA-a), respectively. © 2017 Elsevier Ltd

Wang C.-Q.,Harbin Normal University | Guo S.-Q.,Harbin Normal University | Liu M.,Harbin Normal University | Ru J.,Harbin Normal University | And 2 more authors.
Acta Prataculturae Sinica | Year: 2016

The stomatal structures of the leaves (stems or fruits) of 48 species representing 34 genera of the Apiaceae subfamilies Mackinlayoideae (9 genera) and Azorelloideae (21 genera) and their relative taxa were studied in detail for the first time. Three types of stomata - anomocytic, anisocytic and paracytic - were observed. In Mackinlayoideae, the stomata are mostly paracytic and the minority are anomocytic. In Azorelloideae, stomata are mostly anomocytic and the minority are anisocytic. Cell walls in the Bowlesia clade are deeply waved while they are usually straight in the other clades of Azorelloideae. This stomatal study provides morphological evidence for the two subfamilies and the Bowlesia clade that have been established by molecular research. It also provides evidence to support moving Apiopetalum, Mackinlaya and Stilbocarpa from Araliaceae to Mackinlayoideae and Azorelloideae, and against placing Homalosciadium, Platysace, Hermas and Klotzschia in either of these subfamilies. Copyright © ACTA PRATACULTURAE SINICA. All rights reserved.

Yu R.-P.,Yunnan Academy of Agricultural Sciences | Cheng X.-Y.,Daqing Branch of Heilongjiang Academy of science | Zhang G.-F.,Yunnan University | Li H.,Yunnan Academy of Agricultural Sciences | Gui M.,Yunnan Academy of Agricultural Sciences
Zhiwu Shengli Xuebao/Plant Physiology Journal | Year: 2016

Cibotium barometz (L.) J. Sm., an important group of the endangered ferns, is listed in the Category II of State Special Protected Plants. In present study, the gametophyte development and apogamy of Cibotium barometz were investigated by the sterile culture of spores. The results showed that spores were yellow, tetrahedral and trilete. There were strip ornamentations on the distal surface of spores. The germination type of spores was Vittaria-type. The gametophyte development was mainly Adiantum-type. Antheridia appeared after about 40 days of spore cultivation. Archegonia appeared later than antheridia. Apogamy of Cibotium barometz was facultative, which happened at the apical region of thickened prothallus. Compared with in vitro sporophytic plantlet, in vitro apogamic plantlet had smaller frond and leaflet, and characters of tripinnate leaf was more significant. Hairs on the stipe of apogamic plantlet were denser and longer. Ploidy of apogamic plantlet was half of sporophytic plantlet. © 2016, Science Press. All right reserved.

Liu Y.,University of Science and Technology Beijing | Liu Y.,Daqing Branch of Heilongjiang Academy of Science | Gong A.,University of Science and Technology Beijing | Qiu L.,University of Science and Technology Beijing | And 2 more authors.
MicrobiologyOpen | Year: 2017

Pseudomonas aeruginosa is a good environmental microorganism capable of degrading decabromodiphenyl ether (BDE-209). This paper studied the effect of Cu2+ and humic acid (HA) extracted from e-waste contaminated soils on biodegradation of BDE-209 by P. aeruginosa. The adsorption isotherms of Cu2+ on HA, the crude enzyme activity, cell surface morphology, and biodegradation pathway were also investigated. The results showed that BDE-209 biodegradation by P. aeruginosa was inhibited at Cu2+ concentrations above 5 mg L−1, but exhibited the best effect at the condition of 40 mg L−1 Cu2+ + 3 g L−1 HA. At the condition of 40 mg L−1 Cu2+ + 3 g L−1 HA, 97.35 ± 2.33% of the initial BDE-209 was degraded after 5 days, debromination efficiency was 72.14 ± 1.89%, crude enzyme activity reached the maximum of 0.519 ± 0.022U g−1 protein, cell surface of P. aeruginosa was smooth with normal short-rod shapes, and biodegradation pathway mainly include debromination, hydroxylation, and cleavage of the diphenyl ether bond. It was suggested that soil HA could eliminate the toxic effect of high Cu2+ concentrations and biodegradation of BDE-209 was improved by synergistic effect of HA and Cu2+. © 2017 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

Hu Y.,Northeast Petroleum University | Cao J.,Northeast Petroleum University | Deng J.,China Institute of Technology | Cui B.,Daqing Branch of Heilongjiang Academy of science | And 3 more authors.
Reaction Kinetics, Mechanisms and Catalysis | Year: 2012

We investigated the reductive amination of ethanol to acetonitrile on Cu/γ-Al 2O 3. The different catalysts were characterized by XRF, XRD, H 2-TPR and CO adsorption, and products were identified by FTIR and GC-MS. Our results show that dehydrogenation of ethanol takes place over Cu sites, γ-Al 2O 3 not only plays the role of the carrier and has catalytic dehydration function. Cu sites with high metal area and good stability are necessary to get high yield of acetonitrile. Among the impregnation, coprecipitation and physical mixing methods, impregnation is the most suitable to form Cu sites for meeting the requirements. The phase of copper species and the stability of Cu/γ-Al 2O 3 catalysts are closely related to Cu loading. With the increase of copper loading up to 15%, Cu/γ-Al 2O 3 shows good stability, which is more important for long period reaction. The effect of different reaction conditions such as reaction temperature, ethanol WHSV and ammonia/ethanol molar ratio were also investigated. © Akadémiai Kiadó, Budapest, Hungary 2011.

Acosta-Jurado S.,University of Seville | Navarro-Gomez P.,University of Seville | Murdoch P.D.S.,University of Seville | Crespo-Rivas J.-C.,University of Seville | And 6 more authors.
PLoS ONE | Year: 2016

In the rhizobia-legume symbiotic interaction, bacterial surface polysaccharides, such as exopolysaccharide (EPS), lipopolysaccharide (LPS), K-antigen polysaccharide (KPS) or cyclic glucans (CG), appear to play crucial roles either acting as signals required for the progression of the interaction and/or preventing host defence mechanisms. The symbiotic significance of each of these polysaccharides varies depending on the specific rhizobialegume couple. In this work we show that the production of exopolysaccharide by Sinorhizobium fredii HH103, but not by other S. fredii strains such as USDA257 or NGR234, is repressed by nod gene inducing flavonoids such as genistein and that this repression is dependent on the presence of a functional NodD1 protein. In agreement with the importance of EPS for bacterial biofilms, this reduced EPS production upon treatment with flavonoids correlates with decreased biofilm formation ability. By using quantitative RT-PCR analysis we show that expression of the exoY2 and exoK genes is repressed in late stationary cultures of S. fredii HH103 upon treatment with genistein. Results presented in this work show that in S. fredii HH103 EPS production is regulated just in the opposite way than other bacterial signals such as Nod factors and type 3 secreted effectors: it is repressed by flavonoids and NodD1 and enhanced by the nod repressor NolR. These results are in agreement with our previous observations showing that lack of EPS production by S. fredii HH103 is not only non-detrimental but even beneficial for symbiosis with soybean. © 2016 Acosta-Jurado et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Kan K.,Daqing Branch of Heilongjiang Academy of science | Lin Y.-F.,Heilongjiang University | Wang J.-H.,Institute of Volcanol and Spring of Heilongjiang Academy of science | Tian Y.,Daqing Branch of Heilongjiang Academy of science | And 2 more authors.
Rengong Jingti Xuebao/Journal of Synthetic Crystals | Year: 2015

The carbonates precursor with Mn and Ni were synthesized by precipitation method under low temperature. The MnO2/NiO nanocomposite microspheres were prepared after calcinated treatment. The morphology and structure of materials were studied by SEM and XRD. The results show that the diameter of MnO2/NiO nanocomposite microspheres is about 2 μm. The nanoparticles on the surface of microsphere arrangement sloppy, and there are mesoporous in the port of nanoparticles. The MnO2/NiO-2 thin film sensor has good gas sensing response to NOx at room temperature. When the concentration of NOx reaches 100 ppm, sensitivity is 36% and the response time is 11 s. The limit level of MnO2/NiO-2 sensor to NOx is 3 ppm. ©, 2015, Chinese Ceramic Society. All right reserved.

Yang Y.,Qiqihar University | Yang Y.,Heilongjiang University | Sun Y.-L.,Heilongjiang University | Kan K.,Daqing Branch of Heilongjiang Academy of science | And 4 more authors.
Gongneng Cailiao/Journal of Functional Materials | Year: 2013

1 dimension silica nanofiber with unique big length-diameter ratio, excellent mechanical properties and thermal properties, which is widely used in fields of separation, catalytic and sensor, has been under the favour of scientists. Based on the technique of electrostatic spinning, combined with no template sol-gel method, the uniform diameter and continuous silica nanofibers without many cracks are synthesized. Research shows that: the morphology of synthesized SiO2 nanofiber is good, and the diameter was about 0.5-3 μm, with amorphous structure. Through adjusting aging time of gel to control the gel of viscosity, the shape and structure of silica was managed and SiO2 with bowl shape structure and SiO2 nanofiber with different diameter are obtained at last, which was feasible by using electrostatic spinning method combined with template free sol-gel way.

Liu Y.,University of Science and Technology Beijing | Liu Y.,Daqing Branch of Heilongjiang Academy of Science | Gong A.-J.,University of Science and Technology Beijing | Qiu L.-N.,University of Science and Technology Beijing | And 2 more authors.
International Journal of Environmental Research and Public Health | Year: 2015

The biodegradation effect and mechanism of decabromodiphenyl ether (BDE-209) by crude enzyme extract from Pseudomonas aeruginosa were investigated. The results demonstrated that crude enzyme extract exhibited obviously higher degradation efficiency and shorter biodegradation time than Pseudomonas aeruginosa itself. Under the optimum conditions of pH 9.0, 35 °C and protein content of 2000 mg/L, 92.77% of the initial BDE-209 (20 mg/L) was degraded after 5 h. A BDE-209 biodegradation pathway was proposed on the basis of the biodegradation products identified by GC-MS analysis. The biodegradation mechanism showed that crude enzyme extract degraded BDE-209 into lower brominated PBDEs and OH-PBDEs through debromination and hydroxylation of the aromatic rings. © 2015 by the authors; licensee MDPI, Basel, Switzerland.

PubMed | Heilongjiang Bayi Agricultural University, Harbin Medical University and Daqing Branch of HeiLongJiang Academy of science
Type: | Journal: Microbial pathogenesis | Year: 2016

Our previous study found that the nuclear protein, 68-kDa Src-associated in mitosis protein (Sam68), is translocated to the cytoplasm and forms punctate pattern during enterovirus 71 (EV71) infection [Virus Research, 180 (2014), 1-11]. However, the exact function of this punctate pattern in cytoplasm during EV71 infection remains unknown. In this study, we firstly have examined this punctate pattern of Sam68 re-localization in the cytoplasm, and observed the obvious recruitments of Sam68 to the EV71-induced stress granules (SGs). Sam68, belongs to the KH domain family of RNA binding proteins (RBPs), was then confirmed that its KH domain was essential for this recruitment. Nevertheless, Knockdown of Sam68 expression using ShRNA had no effects on SGs assembly, indicating that Sam68 is not a constitutive component of the SGs during EV71 infection. Lastly, we investigated the importance of microtubulin transport to SGs aggregation, and revealed that microtubule depolymerization inhibited SGs formation, suggesting that EV71-induced SGs move throughout the cytoplasm in a microtubule-dependent manner. Taken together, these results illuminated that EV71 infections can induce SGs formation, and Sam68, as a SGs component, migrates alone with SGs dependent on intact microtubule upon the viral infections. These findings may provide novel underlying mechanism for delineating the role of SGs during EV71 infection.

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