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Zhang H.,Heilongjiang Bayi Agricultural University | Chen N.,Heilongjiang Bayi Agricultural University | Li P.,Harbin Medical University | Pan Z.,Heilongjiang Bayi Agricultural University | And 8 more authors.
Microbial Pathogenesis | Year: 2016

Our previous study found that the nuclear protein, 68-kDa Src-associated in mitosis protein (Sam68), is translocated to the cytoplasm and forms punctate pattern during enterovirus 71 (EV71) infection [Virus Research, 180 (2014), 1–11]. However, the exact function of this punctate pattern in cytoplasm during EV71 infection remains unknown. In this study, we firstly have examined this punctate pattern of Sam68 re-localization in the cytoplasm, and observed the obvious recruitments of Sam68 to the EV71-induced stress granules (SGs). Sam68, belongs to the KH domain family of RNA binding proteins (RBPs), was then confirmed that its KH domain was essential for this recruitment. Nevertheless, Knockdown of Sam68 expression using ShRNA had no effects on SGs assembly, indicating that Sam68 is not a constitutive component of the SGs during EV71 infection. Lastly, we investigated the importance of microtubulin transport to SGs aggregation, and revealed that microtubule depolymerization inhibited SGs formation, suggesting that EV71-induced SGs move throughout the cytoplasm in a microtubule-dependent manner. Taken together, these results illuminated that EV71 infections can induce SGs formation, and Sam68, as a SGs component, migrates alone with SGs dependent on intact microtubule upon the viral infections. These findings may provide novel underlying mechanism for delineating the role of SGs during EV71 infection. © 2016 Elsevier Ltd Source

Hu Y.,Northeast Petroleum University | Cao J.,Northeast Petroleum University | Deng J.,China Institute of Technology | Cui B.,Daqing Branch of HeiLongJiang Academy of science | And 3 more authors.
Reaction Kinetics, Mechanisms and Catalysis | Year: 2012

We investigated the reductive amination of ethanol to acetonitrile on Cu/γ-Al 2O 3. The different catalysts were characterized by XRF, XRD, H 2-TPR and CO adsorption, and products were identified by FTIR and GC-MS. Our results show that dehydrogenation of ethanol takes place over Cu sites, γ-Al 2O 3 not only plays the role of the carrier and has catalytic dehydration function. Cu sites with high metal area and good stability are necessary to get high yield of acetonitrile. Among the impregnation, coprecipitation and physical mixing methods, impregnation is the most suitable to form Cu sites for meeting the requirements. The phase of copper species and the stability of Cu/γ-Al 2O 3 catalysts are closely related to Cu loading. With the increase of copper loading up to 15%, Cu/γ-Al 2O 3 shows good stability, which is more important for long period reaction. The effect of different reaction conditions such as reaction temperature, ethanol WHSV and ammonia/ethanol molar ratio were also investigated. © Akadémiai Kiadó, Budapest, Hungary 2011. Source

Liu Y.,University of Science and Technology Beijing | Liu Y.,Daqing Branch of HeiLongJiang Academy of science | Gong A.-J.,University of Science and Technology Beijing | Qiu L.-N.,University of Science and Technology Beijing | And 2 more authors.
International Journal of Environmental Research and Public Health | Year: 2015

The biodegradation effect and mechanism of decabromodiphenyl ether (BDE-209) by crude enzyme extract from Pseudomonas aeruginosa were investigated. The results demonstrated that crude enzyme extract exhibited obviously higher degradation efficiency and shorter biodegradation time than Pseudomonas aeruginosa itself. Under the optimum conditions of pH 9.0, 35 °C and protein content of 2000 mg/L, 92.77% of the initial BDE-209 (20 mg/L) was degraded after 5 h. A BDE-209 biodegradation pathway was proposed on the basis of the biodegradation products identified by GC-MS analysis. The biodegradation mechanism showed that crude enzyme extract degraded BDE-209 into lower brominated PBDEs and OH-PBDEs through debromination and hydroxylation of the aromatic rings. © 2015 by the authors; licensee MDPI, Basel, Switzerland. Source

Kan K.,Daqing Branch of HeiLongJiang Academy of science | Ge Y.-L.,Heilongjiang University | Lin Y.-F.,Heilongjiang University | Liu S.-Q.,Harbin Normal University | And 2 more authors.
Rengong Jingti Xuebao/Journal of Synthetic Crystals | Year: 2014

To synthesize nanomaterial with good NOx sersor ability at room temperature, three-dimensional (3D) flowerlike Ni(OH)2/CNT nanocomposites were synthesized by one-step reflux method in this paper. The morphology and characteristics of material were studied by XRD, SEM, TEM. The results show that the 3D flower-like nanostructures are actually composed of thin nanosheets, carbon nanotubes embedded in a flower structure. The material has good gas sensing response to NOx at room temperature. The sample with 20 mg CNT has the fastest response and the highest sensitivity to 97 ppm NOx, while the response time is 8 s and sensitivity is 24%, and its limit level is 0.97 ppm. The result of gas mechanism study shows that the additions of CNT increase the composites conductivity and gas transmission capacity. The nanocomposites with 3D structures and a unique chemical composition have significant implications for the practical application of gas sensors and catalysis. Source

Yang Y.,Qiqihar University | Yang Y.,Heilongjiang University | Sun Y.-L.,Heilongjiang University | Yin Y.-B.,Qiqihar University | And 4 more authors.
Gongneng Cailiao/Journal of Functional Materials | Year: 2012

To synthesize nanomaterial with good NOx adsorption ability at room temperature, a porous oblate-Like α-Fe2O3 has been synthesized by the technology of surfactant-assisted nanoparticles assembly and its NOx adsorption behavior at room temperature was studied. The means of XRD, FT-IR, TG-DSC, SEM and TEM were used to investigate the morphology and structure. The result show that every oblate-like Fe2O3 consist of nanoparticles with diameter of 8-10nm assembly, and it belongs to hematite phase of α-Fe2O3 with better crystal. The room temperature NOx adsorption-desorption experiments reveals that the selective NOx adsorption ability of the synthesized material was obviously improved compared with purchase α-Fe2O3. The reason was that O2 in the air was adsorbed on the surface of the oblate-like α-Fe2O3, then O- was formed which can chemical adsorb with NOx molecular. The formation mechanism of NOx adsorption was preliminarily discussed and important experiment basis were introduced for semiconductor oxide at room temperature NOx detection. Source

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