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Lu W.,Huazhong Agricultural University | Liu J.,Huazhong Agricultural University | Liu J.,Dandong Academy of Agricultural science | Xin Q.,Huazhong Agricultural University | And 3 more authors.
Annals of Botany | Year: 2013

Background and Aims Spontaneous male sterility is an advantageous trait for both constructing efficient pollination control systems and for understanding the developmental process of the male reproductive unit in many crops. A triallelic genetic male-sterile locus (BnMs5) has been identified in Brassica napus; however, its complicated genome structure has greatly hampered the isolation of this locus. The aim of this study was to physically map BnMs5 through an integrated map-based cloning strategy and analyse the local chromosomal evolution around BnMs5. Methods A large F2 population was used to integrate the existing genetic maps around BnMs5. A map-based cloning strategy in combination with comparative mapping among B. napus, Arabidopsis, Brassica rapa and Brassica oleracea was employed to facilitate the identification of a target bacterial artificial chromosome (BAC) clone covering the BnMs5 locus. The genomic sequences from the Brassica species were analysed to reveal the regional chromosomal evolution around BnMs5. Key Results BnMs5 was finally delimited to a 0.3-cM genetic fragment from an integrated local genetic map, and was anchored on the B. napus A8 chromosome. Screening of a B. napus BAC clone library and identification of the positive clones validated that JBnB034L06 was the target BAC clone. The closest flanking markers restrict BnMs5 to a 21-kb region on JBnB034L06 containing six predicted functional genes. Good collinearity relationship around BnMs5 between several Brassica species was observed, while violent chromosomal evolutionary events including insertions/deletions, duplications and single nucleotide mutations were also found to have extensively occurred during their divergence. Conclusions This work represents major progress towards the molecular cloning of BnMs5, as well as presenting a powerful, integrative method to mapping loci in plants with complex genomic architecture, such as the amphidiploid B. napus. © The Author 2012. Source


Wang Y.,Northeast Normal University | Wang Y.,CAS Changchun Northeast Institute of Geography and Agroecology | Jiang H.,Northeast Normal University | He C.,Northeast Normal University | And 4 more authors.
Ecological Engineering | Year: 2016

Soil nematode community composition could change following the conversion of wetlands to agricultural fields. In Jinchuan Wetland of Changbai Mountain area, China, we investigated the soil nematode community in different types of wetland-farmland interaction. Our results suggest that the Shannnon-Weaver diversity index in OW, R30, R1, KN, KP and PF are 2.48, 1.65, 1.70, 1.68, 1.39, 1.98, respectively, which means the native wetland had great biodiversity abundance. The genus of nematodes initially increased upon recovery from farmland, and nematode densities decreased in the tillage and cropping wetlands. Wilsonem and Prismatolaimus were sensitive to tillage. The bacterial-feeder nematodes increased after the first tillage treatment, while the nematode community was in a relatively stable state after a return to wetland following 30 years of agriculture. Plant parasite index (PPI) had about tenfold reduced in paddy farmland in comparison to the original wetland, and the values were positively associated with the change extent of recovery to reclamation of wetland. The study showed that agricultural activities disturbed the diversity of soil nematodes in Jinchuan Wetland. Certain kinds of genus and trophic diversity of the nematode community could indicate the effects of paddy cultivation on the wetland soil ecosystem. © 2016 Elsevier B.V. Source


Jiang S.,ShenYang Agricultural University | Zhang X.,Dandong Academy of Agricultural science | Wang J.,ShenYang Agricultural University | Chen W.,ShenYang Agricultural University | Xu Z.,ShenYang Agricultural University
Euphytica | Year: 2010

The morphological traits of leaves, such as size and shape, are major determinants of plant architecture and strongly affect high yield performance. To understand the molecular mechanism governing flag leaf length, we analyzed quantitative trait loci (QTLs) affecting flag leaf length by employing 176 F2 individuals derived from a cross between two japonica rice cultivars: Shennong265 (SN265) and Lijiangxintuanheigu (LTH). We identified qFLL3, qFLL6 and qFLL9 from this F2 population. Flag leaf length was increased by SN265 alleles at qFLL3 and qFLL6, but by LTH allele at qFLL9. In order to eliminate the influence of qFLL3 and qFLL6, one single residual heterozygous plant for qFLL9 region, RH-qFLL9, was selected based on the genotypes of 114 simple sequence repeat (SSR) markers and used as the parent of a segregating population. Using this segregating population of 889 plants, this region was narrowed down to an interval between RM24423 and RM24434. According to the rice annotation project database, there are 17 predicted genes in the 198-kb target region. © 2010 Springer Science+Business Media B.V. Source


Huang G.-T.,ShenYang Agricultural University | Ma S.-L.,ShenYang Agricultural University | Bai L.-P.,ShenYang Agricultural University | Zhang L.,Key Laboratory of Agricultural Biotechnology of Liaoning Province | And 5 more authors.
Molecular Biology Reports | Year: 2012

Abiotic stresses, especially cold, salinity and drought, are the primary causes of crop loss worldwide. Plant adaptation to environmental stresses is dependent upon the activation of cascades of molecular networks involved in stress perception, signal transduction, and the expression of specific stress-related genes and metabolites. Plants have stress-specific adaptive responses as well as responses which protect the plants from more than one environmental stress. There are multiple stress perception and signaling pathways, some of which are specific, but others may cross-talk at various steps. In this review article, we first expound the general stress signal transduction pathways, and then highlight various aspects of biotic stresses signal transduction networks. On the genetic analysis, many cold induced pathways are activated to protect plants from deleterious effects of cold stress, but till date, most studied pathway is ICE-CBF-COR signaling pathway. The Salt-Overly-Sensitive (SOS) pathway, identified through isolation and study of the sos1, sos2, and sos3 mutants, is essential for maintaining favorable ion ratios in the cytoplasm and for tolerance of salt stress. Both ABA-dependent and -independent signaling pathways appear to be involved in osmotic stress tolerance. ROS play a dual role in the response of plants to abiotic stresses functioning as toxic by-products of stress metabolism, as well as important signal transduction molecules and the ROS signaling networks can control growth, development, and stress response. Finally, we talk about the common regulatory system and cross-talk among biotic stresses, with particular emphasis on the MAPK cascades and the cross-talk between ABA signaling and biotic signaling. © 2011 Springer Science+Business Media B.V. Source


Jiang S.-K.,ShenYang Agricultural University | Huang C.,ShenYang Agricultural University | zhang X.-J.,ShenYang Agricultural University | zhang X.-J.,Dandong Academy of Agricultural science | And 3 more authors.
Agricultural Sciences in China | Year: 2010

To select highly informative microsatellite markers (SSRs) and establish a useful genetic SSR framework for rice genotyping, 15 rice (Oryza sativa L.) cultivars including six indica varieties and nine japonica varieties were used to analyze the polymorphism information content (PIC) value of 489 SSR markers. A total of 1 296 alleles were detected by 405 polymorphic markers with an average of 3.2 per locus. The PIC value of each chromosome was ranged from 0.4039 (chromosome 2) to 0.5840 (chromosome 11). Among the two rice subspecies, indica (0.3685-0.4952) gave a higher PIC value than japonica (0.1326-0.3164) and displayed a higher genetic diversity. Genetic diversity of indica was high on chromosome 12 (0.4952) and low on chromosome 8 (0.3685), while that for japonica was high on chromosome 11 (0.3164) and low on chromosome 2 (0.1326). A SSR framework including 141 highly informative markers for genotyping was selected from 199 SSR markers (PIC > 0.50). Ninety-three SSR markers distributed on 12 chromosomes were found to be related to indica-japonica differentiation. Of these 93 pairs of SSR primers, 17 pairs were considered as core primers (all the japonica varieties have the same specific alleles, while the indica varieties have another specific alleles), 48 pairs as the second classic primers (all the japonica or indica varieties have the same specific alleles, while the indica or japanica varieties have two or more other specific alleles) and 28 pairs as the third classic primers (all the japonica and indica varieties have two or more alleles, but the specific alleles are different between japonica and indica). Thirty-two SSR markers were selected to be highly informative and useful for genetic diversity analysis of japonica varieties. This work provides a lot of useful information of SSR markers for rice breeding programs, especially for genotyping, diversity analysis and genetic mapping. © 2010 Chinese Academy of Agricultural Sciences. Source

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