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Damietta, Egypt

El-Mezayen H.A.,Helwan University | Metwally F.M.,National Research Center of Egypt | Darwish H.,Damietta Cancer Institute
Tumor Biology | Year: 2014

Invasion and metastasis of solid tumors require proteolytic enzymes for degradation of the basal membrane and extracellular matrix. Currently, there are no reliable methodologies to predict the risk for metastatic disease. In this context, our aim has been focused on the development of a noninvasive score based on tumor-associated trypsin inhibitor (TATI) for the assessment of metastasis in patients with breast cancer. TATI, trypsin, and soluble epidermal growth factor receptor (sEGFR) were assayed by enzyme-linked immunosorbent assay. CA 15.3 serum level was assayed by microparticle enzyme immunoassay in 265 patients with breast cancer. Statistical analyses were performed by logistic regression and receiver operating characteristic analysis curves. Using multivariate discriminant analysis, a score is selected based on absolute values of the four biochemical markers: TATI-metastatic breast cancer score (TATI-MBCS) = [0.03 × CA 15.3 (U/L) + 0.039 × TATI (ng/ml) + 0.04 × trypsin (ng/ml) + 0.023 × sEGFR (ng/ml) - 6.49 (numerical constant)]. This function correctly classified 84 % of metastatic breast cancer at cutoff value = 0.62 (i.e., greater than 0.62 indicates patients with metastatic breast cancer and less than 0.62 indicates patients with nonmetastatic breast cancer). In conclusion, TATI-MBCS is a novel, noninvasive, and simple score which can be applied to discriminate patients with metastatic breast cancer. © 2013 International Society of Oncology and BioMarkers (ISOBM). Source


El-Mezayen H.A.,Helwan University | Darwish H.,Damietta Cancer Institute
Tumor Biology | Year: 2014

Hepatocellular carcinoma (HCC) is often diagnosed at advanced stage where effective therapies are lacking. Identification of new scoring system is needed to discriminate HCC patients from those with chronic liver disease. Based on the link between vascular endothelial growth factor (VEGF) and HCC progression, we aimed to develop a novel score based on combination of VEGF and routine laboratory tests for early prediction of HCC. VEGF was assayed for HCC group (123), liver cirrhosis group (210), and control group (50) by enzyme-linked immunosorbent assay (ELISA). Data from all groups were retrospectively analyzed including α-fetoprotein (AFP), international normalized ratio (INR), albumin and platelet count, transaminases, and age. Areas under receiving operating curve (ROC) were used to develop the score. A novel index named hepatocellular carcinoma-vascular endothelial growth factor score (HCC-VEGF score)=1.26 (numerical constant+0.05×AFP (U l-1)+0. 038×VEGF (ng ml-1)+0.004×INR-1.02×albumin (g l -1)-0.002×platelet count×109 l-1 was developed. HCC-VEGF score produce area under ROC curve of 0.98 for discriminating HCC patients from liver cirrhosis with sensitivity of 91 % and specificity of 82 % at cutoff 4.4 (i.e., less than 4.4 considered cirrhosis and greater than 4.4 considered HCC). Hepatocellular carcinoma-VEGF score could replace AFP in HCC screening and follow up of cirrhotic patients. © 2014 International Society of Oncology and BioMarkers (ISOBM). Source


Makhlouf M.M.,Taif University | Makhlouf M.M.,Damietta University | Makhlouf M.M.,Damietta Cancer Institute | Zeyada H.M.,Damietta University
Synthetic Metals | Year: 2016

3-phenyl azo-4-hydroxycoumarin, PAHC, compound was synthesized by reacting aniline diazonium salt with 4-hydroxy coumarin. The thin films of PAHC were prepared on the glass and quartz substrates by thermal evaporation technique under vacuum pressure 10-5 mbar. The structure of thin films was characterized using X-ray diffraction, XRD, and Fourier transformation infrared, FTIR, techniques. The XRD pattern for the pristine PAHC thin film showed nanocrystallites distributed in amorphous matrix. Annealing at 423 K improved the crystallinity of films and the crystallite size is in the range 23-36 nm. FTIR showed different vibrational modes, observed in infrared spectra of the powder, pristine and annealed thin films, were assigned to the molecular bonding structure of PAHC compound. The optical properties of PAHC thin films were investigated by the spectrophotometer measurements of the pristine and annealed PAHC films in the range 200-2100 nm. The refractive and absorption indices of the pristine and annealed PAHC films were calculated from measured transmittance and reflectance data. The results showed that the refractive and absorption indices of thin films are influenced with increasing annealing temperature. The direct allowed transitions were the most probable transition and the optical band gap was found to vary from 2.14 to 2.21 eV with increasing annealing temperature. The single oscillator model was applied in the normal region of dispersion spectrum to determine the dispersion parameters and it is noted that they are decreased with annealing temperature. The AC electrical conductivity, σAC, and the real and imaginary parts of dielectric constants measurements were investigated in temperature range 304-413 K and frequency range 0.1-100 kHz; they confirmed semiconductor behavior of the PAHC films. Source


El-Mezayen H.A.,Helwan University | Toson E.-S.A.,Damietta University | Darwish H.,Damietta Cancer Institute | Metwally F.M.,National Research Center of Egypt
Medical Oncology | Year: 2013

Tumor metastasis involves the dissemination of malignant cells into the basement membrane, and the vascular system contributes to the circulating pool of these markers. In this context, our aim has been focused on the development of a non-invasive score based on degradation of the backbone of glycosaminoglycans of the extracellular matrix; namely hyaluronic acid (HA), for the assessment of metastasis in patients with breast cancer. HA level was determined by enzyme-linked immunosorbent assay; CA 15.3 was determined by microparticle enzyme immunoassay; hyaluronidase, N-acetyl-β-d- glucosaminidase, β-glucuronidase, glucuronic acid, and glucosamine were assayed by standard colorimetric techniques in 217 patients with breast cancer. Statistical analyses were performed by logistic regression and receiver-operating characteristic analysis curves. The multivariate discriminant analysis selects a score based on absolute values of the six biochemical markers: metastatic breast cancer score (MBCS) = [1.04 (Numerical constant) + 0.003 × CA 15.3 (U/l) + 0.001 × HA (ng/ml) + 0.004 × hyaluronidase (mg N-acetyl-β-d-glucosamine/ml/18 h) + 0.001 × N-acetyl-β-d-glucosaminidase (μmol/ml/min) + 0.026 × glucuronic acid (ng/ml) + 0.003 × glucosamine (μg/dl)]. This function correctly classified 87 % of metastatic breast cancer at cut-off value = 0.85 (i.e., great than 0.85 indicates patients with metastatic breast cancer and less than 0.85 indicates patients with non-metastatic breast cancer). MBCS is a novel, non-invasive, and simple score which can be applied to discriminate patients with metastatic breast cancer. © 2012 Springer Science+Business Media New York. Source


Makhlouf M.M.,Taif University | Makhlouf M.M.,Damietta Cancer Institute | El-Nahass M.M.,Ain Shams University
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy | Year: 2015

In this work, we report on the effect of γ-ray irradiation and annealing temperature on the optical properties of metal-free tetraphenylporphyrin, H2TPP, and zinc tetraphenylporphyrin, ZnTPP, thin films. Thin films of H2TPP and ZnTPP were successfully prepared by the thermal evaporation technique. The optical properties of H2TPP and ZnTPP films were investigated using spectrophotometric measurements of the transmittance and reflectance at normal incidence of light in the wavelength range from 200 to 2500 nm. The absorption spectra of H2TPP showed four absorption bands, namely the Q, B, N and M bands. The effect of inserting Zn atom into the cavity of porphyrin macrocycle in ZnTPP molecule distorted the Q and B bands, reduced the width of absorption region and influenced the optical constants and dispersion parameters. In all conditions, the type of electron transition is indirect allowed transition. Anomalous dispersion is observed in the absorption region but normal dispersion occurs in the transparent region of spectra. We adopted multi-oscillator model and the single oscillator model to interpret the anomalous and normal dispersion, respectively. We have found that the annealing temperature has mostly the opposite effect of γ-ray irradiation on absorption and dispersion characteristics of these films. © 2015 Elsevier B.V. All rights reserved. Source

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