Dalmia Center for Research and Development

Coimbatore, India

Dalmia Center for Research and Development

Coimbatore, India
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Kotte S.C.B.,Jawaharlal Nehru Technological University | Ramakoteswara Rao C.,Jawaharlal Nehru Technological University | Tulam V.K.,Jawaharlal Nehru Technological University | Raghavan S.,Dalmia Center for Research and Development | And 2 more authors.
International Journal of Research in Pharmaceutical Sciences | Year: 2012

A simple, fast, novel and reproducible analytical method by using High-performance liquid chromatographic (HPLC) /High resolution mass spectrometry was proposed. Quadrupole Time of Flight coupled with Evaporative Light Scattering Detector (ELSD), Photo Diode Array detector and dual Electrospray ionization (dual ESI) multistage tandem mass spectrometry (LC-MS) were used to identify of Gefitinib in tablet dosage form for validated and estimation. Efficient Chromatographic separation of analytes was achieved on Agilent XDB-C18 column (4.6 x 50mm, 1.8um) using an isocratic elution mode with a mobile phase comprised acetonitrile and 0.1% formic acid in water (75:25, v/v). The flow rate was 500 μL/min and effluents were monitored at 250nm.The chromatographic run time was 2 min. The column effluents were detected by Agilent Accurate mass Q-TOF LC/MS 6500 series mass spectrometer using dual electrospray ionization (ESI) in negative mode (445.1299m/z and chlorine adduct 481.1056m/z).The developed LCMS method is validated with respect to linearity, accuracy, precision, specificity, limit of detection, limit of quantification and robustness. The method was observed to be limit-of-detection (LOD) and limit-of-quantification (LOQ) was found to be 5.56ppm and 11.12ppm respectively. The method was success-fully used for applied for the qualitative determination of Gefitinib in tablet formulation. © JK Welfare & Pharmascope Foundation.


Kumar A.S.S.,Dalmia Center for Research and Development | Bose K.S.C.,Evolva Biotech Private Ltd | Kumar K.V.T.S.P.,Dalmia Center for Research and Development | Raghavan S.,Dalmia Center for Research and Development | And 2 more authors.
Asian Journal of Chemistry | Year: 2014

Plant derived bio products have become integral part of world health care system which not only include botanical drugs but also agro chemicals, cosmetics, nutraceuticals, flavors and fragrances and so on. The World Bank estimates that global trade in plant derived products will grow at average 10-12 % per year and will reach whooping USD 123 billion by 2020. Inevitably this brought attention to our sthala vriksha or sacred tree neem (Azadirachta indica) considered as store house of high value products with vast array of biological activities like insecticidal, spermicidal, anticancer, hypoglycemic, antiulcer, antiinflammatory, and many more. However due to inherent complexities in scalability which includes consistent supply chain and difficulty in synthesizing bioactive metabolites using established chemical routes and seasonal variation in bio-efficacy with change in plant chemistry, place, age etc. makes large scale production of most of them is impossible. Hence there is need for a paradigm shift in combining traditional approaches with more sophisticated biotechnological methods to produce these products in heterologous system, thereby enabling a consistent scale-up at a commercial level. This article reviews the terpenoids reported in neem so far, their proposed biosynthetic pathways and potential avenues for expressing them in microbial cell factories.


Pavan Kumar K.V.T.S.,Dalmia Center for Research and Development | Chinta R.K.,Jawaharlal Nehru University | Raghavan S.,Dalmia Center for Research and Development | Murali P.M.,Dalmia Center for Research and Development
International Journal of Pharmaceutical Sciences Review and Research | Year: 2012

Diabetes Mellitus, One of the life style disorders, has become common problem in current world scenario and creating health "Tsunami" due to un-availability of cost effective treatment. The role of Dipeptidyl peptidase IV receptor, an anti-diabetic drug target, involved in incretin metabolism has provided an opportunity to counteract type2 diabetes by inhibiting its action on glucagon like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP). Plants are one the major sources for natural products with various therapeutic activities and ancient Indian traditional medicine Ayurveda uses many of these plant ingredients to treat several human ailments. A Xanthone class compound, glucosylxanthone, is one of such natural compound from plant that has various biological activities. In the current effort glucosylxanthone and its analogues are studied for insilico inhibitory activity of DPPIV receptor along with known DPPIV inhibitors that includes molecules in various phases of drug discovery pipeline by using MOLA, tool for Virtual Screening using AutoDock4/Vina in a computer cluster using non-dedicated multi-platform computers. Glucosylxanthone and its analogues have shown good comparable insilico binding activity with FDA approved drugs and other molecules currently under development for DPPIV inhibitory activity as anti-diabetic therapy.


Kotte S.C.B.,Jawaharlal Nehru Technological University | Tulam V.K.,Jawaharlal Nehru Technological University | Chinta R.R.,Jawaharlal Nehru Technological University | Shriram Raghavan S.,Dalmia Center for Research and Development | And 2 more authors.
International Journal of ChemTech Research | Year: 2012

A simple, fast, novel method with dual Electrospray ionization (dual ESI), multistage tandem mass spectrometry (LC-MS) were used to identify of Amoxicillin, Ampicillin and Cephalexin. The established method with excellent separation and good capacity factorwas successfully applied. Ion detection was performed usingQuadrupole -Time of Flight coupled with dual ESI ion sourceand identified corresponding ions as m/z 365, 349, 347[M+H] with respective of Cephalexin, Amoxicillin, Ampicillin. The results of the study showed that the proposed LCMS method is simple, rapid, precise and accurate, which is useful for the routine determination of Cephalexin, Amoxicillin, and Ampicillinin bulk drug and in its pharmaceutical dosage forms.

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