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Zhang L.-L.,Liaoning Medical University | Zhang J.-Y.,Dalian University | Qu Z.,Dalian Stomatological Hospital | Song K.-D.,Dalian University of Technology | Ai H.-J.,Liaoning Medical University
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: Diabetes can reduce the success rate of implants by affecting osteogenesis. What’s more, it also causes a variety of complications. Reduced glutathione can remove free radicals directly or indirectly by an enzymatic reaction, and thus plays a protective role on osteoblasts. OBJECTIVE: To evaluate the effects of reduced glutathione on the proliferation and differentiation of osteoblasts under high glucose conditions and the influence on bone defects of diabetes rabbits. METHODS: In this experiment, the osteoblasts were divided into three groups: normal control group, high glucose group, high glucose+reduced glutathione group. We observed the proliferation and differentiation of osteoblasts by alkaline phosphatase staining and MTT. The diabetes rabbits which had bone defects were divided into two groups, and the mixture of reduced glutathione and Bioss was used to fill the bone defects in the experimental group to judge the influence of reduced glutathione on osteogenesis based on X-ray observation. RESULTS AND CONCLUSION: Reduced glutathione could effectively promote the proliferation and differentiation of osteoblasts under high glucose conditions. The cell proliferation and differentiation showed more significant increase in the high glucose+reduced glutathione group than the high glucose group (P < 0.05). The bone mineral density shadow of the tibial bone defects after treatment with the mixture of reduced glutathione and Bioss was significantly higher than that after treatment with single Bioss (P < 0.05). These findings indicate that reduced glutathione can promote the proliferation and differentiation of osteoblasts, and effectively promote the repair of bone defects. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.

Hou Y.-Y.,Dalian Medical University | Zhen Y.-H.,Dalian Medical University | Wang D.,Dalian Stomatological Hospital | Zhu J.,Dalian Medical University | And 6 more authors.
Journal of Applied Microbiology | Year: 2014

Aims: To investigate the effects of an egg yolk-derived immunoglobulin (IgY) specific to Prevotella intermedia in vitro and in vivo. Methods and Results: An IgY specific to P. intermedia was produced by immunizing hens with formaldehyde-inactivated P. intermedia and showed high titres when subjected to an ELISA. The obtained IgY inhibited the growth of P. intermedia in a dose-dependent manner at concentrations from 1 to 20 mg ml-1 in Center for Disease Control and Prevention liquid medium. Forty rats were challenged with P. intermedia on gingivae and then randomly divided into four groups, which were syringed respectively with phosphate-buffered saline, 1 mg ml-1 of tinidazole, 20 mg ml-1 of nonspecific IgY and 20 mg ml-1 of the IgY specific to P. intermedia at a dosage of 300 μl per day. Gingival index (GI), plaque index (PI), bleeding on probing (BOP), counts of white blood cell (WBC) and histopathological slide of the gums were measured after treatment for 15 days. The gingivitis rats treated with the IgY specific to P. intermedia showed significantly decreased GI, PI, BOP and WBC (P < 0·05). Gum histopathology of the treated rats demonstrated a superior protective effect of the specific IgY on P. intermedia-mediated gingivitis. Conclusions: A new immunoglobulin specific to P. intermedia was developed from egg yolk. This specific IgY can dose-dependently inhibit the growth of P. intermedia and protect rats from gingivitis induced by P. intermedia. Significance and Impact of the Study: The new IgY has potential for the treatment of P. intermedia-mediated gingivitis. © 2013 The Society for Applied Microbiology.

Thevissen P.W.,Catholic University of Leuven | Alqerban A.,Riiyadh Military Hospital RKH | Asaumi J.,Okayama University | Kahveci F.,Gulumse Agiz Ve Dis Sagligi Klinigi | And 7 more authors.
Forensic Science International | Year: 2010

Unquestionable forensic age investigations are based on statistical models constructed on a sample containing subjects of identical origin as the examined individual. In cases where corresponding models are unavailable, the established report has to describe the possible effects of this unrelated information on the predicted age outcome. The aim of this study is to collect country specific databases of third molar development and to verify how the related dental age estimations are influenced if we were to use dental developmental information only from Belgium or from all collected countries together.Data containing third molar developmental stages scored following Gleiser and Hunt (modified by Köhler) were collected from 9 country specific populations (Belgium, China, Japan, Korea, Poland, Thailand, Turkey, Saudi-Arabia and South-India). Age predictions were obtained from a training dataset and validated on a test dataset. Bayes rule using the repeated third molar scores is applied to get age predictions and prediction intervals. Three age predictions were compared for males and females separately. For the first prediction, the training dataset contains only Belgian subjects. For the second prediction, the training dataset for each country consists only of subjects of the country itself. For the final prediction, subjects from all countries are pooled into one common training dataset. Besides the (absolute) difference between the chronological age and the predicted age, specific interest lies in the juvenile-adult distinction.In the age range from 16 to 22 years 6982 subjects (3189 male and 3793 female) were analyzed.Using information on third molar development from Belgium compared to information from the country specific databases hardly increased the mean absolute differences (MAD) and mean squared errors (MSE): the MAD and MSE increased on average with 0.5 and 2.5 months with maximal increases of, respectively 1.6 and 7.3 months.Using information from all countries pooled compared to country specific information provided even on average negligible increases (0.05 and 0.2 months for MAD and MSE, respectively). For the juvenile-adult discrimination, using information from all countries instead of country specific information yielded comparable performances. Using Belgium instead of country specific information increased the percentage of correctly identified juveniles, but decreased the percentage of correctly identified adults.The adult-juvenile discrimination based on information used from Belgium provides judicially the best applied reference. © 2010 Elsevier Ireland Ltd.

Qu Z.,Dalian Stomatological Hospital | Zhang J.-Y.,Dalian University | Guo Y.,Dalian Stomatological Hospital | Ma W.-D.,Dalian Stomatological Hospital | Ma L.,Dalian Stomatological Hospital
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: Numerous studies have confirmed that enamel matrix proteins can promote the regeneration of osteoblasts and cementoblast, and then it can achieve approaching physiological periodontal regeneration in the treatment of periodontal defects. OBJECTIVE: To observe the effects of different concentrations of enamel matrix proteins on proliferation, viability, differentiation and migration of human periodontal ligament cells. METHODS: The human periodontal ligament cells at the third generation were gained, and then cultured in serum-free DMEM containing different concentrations of enamel matrix proteins (0, 12.5, 25, 50, 100, 250 mg/L). After 24 hours of culture, proliferation and viability of periodontal ligament cells were measured using [(3)H]-thymidine uptake and MTT assay. After 48 hours of culture, alkaline phosphatase activity and osteocalcin production were detected with commercial available test kits. When the cells grew as a monolayer, the cell culture fluid was removed, and then with a pipette head, a cell incision, 1 mm wideness, was prepared in a monolayer of cells to further observe the cell fusion continuously within 24 hours. RESULTS AND CONCLUSION: The proliferation, viability and differentiation of periodontal ligament cells were gradually increased with the concentration increase of enamel matrix proteins (0-100 mg/L). When the concentration of enamel matrix proteins was 250 mg/L, these parameters began to decrease, but the levels were still higher than those in the 0 mg/L enamel matrix protein group. In the 100 mg/L group, the cells in the wound edge began to grow towards the center in the initial 6 hours, then became confluent at 12 hours, and until the 20th hour of culture, the cells in the two sides of the wound edge were completely fused to fully close the wound edge, indicating a better wound healing in the 100 mg/L group than the other groups. These findings suggest that enamel matrix proteins can stimulate proliferation, viability, differentiation and migration of periodontal ligament cells. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.

Liu L.-J.,Dalian Stomatological Hospital | Liu M.-L.,Dalian Stomatological Hospital
Journal of Dalian Medical University | Year: 2013

Objective: To evaluate clinical effects of indirect polymerid porcelain inlay on massive defect of vital pulp posterior teeth. Methods: From 2008 January to 2010 March, 31 massive defect vital pulp posterior teeth were restored with Ceramage polymerid porcelain inlays and cemented with RelyX Unicem resin adhensive. The modified USPHS criteria were used to evaluate the treatment effects at recall periods. Follow-up were conducted for two years. Results: After 2 years, the success rate was 100%. All of observation items in modified USPHS criteria were above C level at recall periods. Conclusion: Massive defect of vital pulp posterior teeth can be well restored with the indirect polymerid porcelain inlays. It indicates indirect polymerid porcelain inlay used for massive defect of vital pulp posterior teeth have stable Clinical effects.

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