PubMed | Beijing Jishuitan Hospital, Dalian Sixth Peoples Hospital, Peking University and China National Petroleum Corporation
Type: Journal Article | Journal: Malaria journal | Year: 2016
Malaria is the main health risk for Chinese expatriates working in Niger. Health education is a recommended intervention for prevention of malaria among non-immune travellers and expatriate workers. It is urgent to develop an effective and feasible way for these populations to obtain information about the prevention and treatment of malaria.An individually randomized, unblinded, controlled trial was used to evaluate the effectiveness of using WeChat official accounts for health education to improve malaria health literacy among Chinese expatriates in Niger. A total 1441 participants completed a baseline malaria health literacy questionnaire and were randomly assigned to an intervention or comparison group in a ratio of 1:1. From July to October 2014, 50 malaria prevention and treatment messages were sent to the intervention group; 50 health news messages were concurrently sent to the control group. Both groups completed the malaria health literacy questionnaire again 4 months after the start of the education intervention. A questionnaire addressing satisfaction with the health education programme was completed by the intervention group. Malaria morbidity data for 2013 and 2014 were also collected.At baseline, participant health literacy rates were 58.29, 62, 54, and 34% for skills, knowledge, practice, and attitude, respectively. After the intervention, rates for all four aspects of malaria literacy were above 70%. There was greater change in knowledge, attitude, practice, skills, and overall health literacy among the intervention group compared with the controls, with a statistically significant difference (p<0.01). This was especially true for acquisition of malaria-related knowledge, practice and attitude; comprehensive intervention practices; and, correct use of rapid diagnostic tests (p<0.001). The reported malaria morbidity during the study period decreased from 23.72 to 15.40%. Participants reported high levels of satisfaction with the WeChat health education programme with over 80% stating that they would continue to follow the programme.The present health education intervention, via a WeChat official account, for the prevention and treatment of malaria among non-immune travellers and expatriate workers proved to be an effective, sustainable, feasible, and well accepted strategy for improving malaria health literacy among Chinese expatriates in Niger.
Xu Y.-Y.,Binzhou Medical University |
Jiang N.,Dalian Sixth Peoples Hospital |
Liu T.-S.,Binzhou Medical University |
Qu H.-Q.,Binzhou Medical University |
Wang T.,Yantai University
Molecular Medicine Reports | Year: 2012
Cisplatin (cis-diamminedichloroplatinum, CDDP) is one of the most potent anticancer drugs. However, the therapeutic value of CDDP is greatly compromised by its dose-limiting nephrotoxicity. This study was performed to investigate whether reduced glutathione (GSH) was able to reduce the kidney injury induced by CDDP and whether it affected the anticancer activity of CDDP in vivo and in vitro. In in vivo experiments, mice were divided into five groups: control, CDDP only and three GSH treatment groups. Blood samples were collected 72 h after CDDP administration to determine the levels of blood urea nitrogen (BUN) and plasma creatinine (Cr). In addition, we examined antioxidative parameters, malondialdehyde (MDA) levels and histopathological changes in the kidney. In order to investigate whether GSH affected the anticancer activity of CDDP, we performed a sulforhodamine B (SRB) assay to determine the antiproliferative effect in three tumor cell lines of treatment with CDDP alone or combined with GSH and examined the cell morphology. The results revealed that GSH decreased the BUN and Cr levels in plasma, ameliorated the pathological changes induced by CDDP and enhanced the endogenous antioxidant capacities in all three GSH groups. Furthermore, GSH significantly inhibited the growth of the three tumor cell lines when combined with CDDP and did not affect the inhibitory effect of CDDP on the carcinoma cell proliferation. In addition, we found no differences among the three GSH groups. These findings suggest that GSH is able to attenuate the nephrotoxicity induced by CDDP, not only when administered prior to CDDP, but also when administered at the same time as or subsequent to CDDP administration, without affecting the anticancer activity of CDDP. Thus, the administration of GSH is a promising approach for attenuating the nephrotoxicity caused by CDDP.
PubMed | Monash University, Zhengzhou University, Dalian Sixth Peoples Hospital, Deakin University and 2 more.
Type: | Journal: Cancer letters | Year: 2016
As one of the life-threatening diseases involving multi-step genetic and epigenetic disorders, cancer has long been a dynamic research area for siRNA-based therapy as half of the current siRNA-based clinical trials are involved in oncology. However, despite consistent enthusiasm in the academic world, siRNA-based cancer treatment still faces obstacles and difficulties in clinical development. In this article, we discuss key challenges facing siRNA-based cancer treatment revealed from recent clinical and preclinical studies, including chemical modification, tumour penetration, endosomal escape, target selection and off-target effects. In addition, opportunities and avenues for translating siRNA technology from bench to oncologic clinics are explored.
Wang L.,Zhejiang University of Technology |
Wang L.,CAS Dalian Institute of Chemical Physics |
Hu C.,CAS Dalian Institute of Chemical Physics |
Liu S.,Dalian Municipal Central Hospital |
And 5 more authors.
Journal of Proteome Research | Year: 2016
Chronic kidney disease (CKD) has been a global health problem that has a great possibility of being developed into uremia in the end. Hemodialysis (HD) is the most commonly used strategy for treating uremic patients; however, the patients still have a high risk of suffering various complications. It is well recognized that lipid disorder usually occurs in maintenance HD patients. To systemically study the effects of HD on lipid metabolism associated with uremia, we employed an ultraperformance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS)-based lipidomics method. A total of 87 human plasma samples from patients with prehemodialysis (pre-HD)/posthemodialysis (post-HD) treatment and the healthy controls were enrolled in the study. As compared with pre-HD patients, many plasma lipids showed significant changes (p < 0.05) in patients receiving HD therapy. Specifically, sum of free fatty acids (FFA) as well as saturated FFA and eicosanoids and sums of lyso-phosphatidylinositols and lyso-phosphatidylethanolamines, FFA 16:1/FFA 16:0, and FFA 18:1/FFA 18:0 were obviously higher in the pre-HD group than in the controls while they were significantly lower in patients after HD. These results indicated that UPLC-Q-TOF/MS-based lipidomics is a promising approach to investigate lipid alterations in relation to uremia and it is helpful to understand complex complications involved in HD patients. © 2016 American Chemical Society.
Wang Z.,Dalian University of Technology |
Wei H.,Dalian University of Technology |
Jia L.,Dalian University of Technology |
Xu L.,Dalian University of Technology |
And 2 more authors.
Transfusion and Apheresis Science | Year: 2012
A water-soluble adsorbent was developed for removing bilirubin from the plasma of hyperbilirubinemia patient. The adsorbent was synthesized by grafting β-cyclodextrin (β-CD) to branched polyethyleneimine (PEI) matrix. The resulting β-CD-PEI polymer had an average molecular weight of 163.7. kD, and it contained 56.3 β-CD functional groups. In β-CD-PEI-spiked dialysis, 35.8% of plasma bilirubin was removed, which was higher than that removed by the same concentration of bovine serum albumin. β-CD-PEI also removed aromatic amino acids and bile acids. The results indicated that β-CD-PEI could be an effective adsorbent for blood purification application aiming at the removal of toxins. © 2012.
Shigdar S.,Deakin University |
Qian C.,Deakin University |
Lv L.,Dalian Medical University |
Pu C.,Dalian Sixth Peoples Hospital |
And 6 more authors.
PLoS ONE | Year: 2013
EpCAM is expressed at low levels in a variety of normal human epithelial tissues, but is overexpressed in 70-90% of carcinomas. From a clinico-pathological point of view, this has both prognostic and therapeutic significance. EpCAM was first suggested as a therapeutic target for the treatment of epithelial cancers in the 1990s. However, following several immunotherapy trials, the results have been mixed. It has been suggested that this is due, at least in part, to an unknown level of EpCAM expression in the tumors being targeted. Thus, selection of patients who would benefit from EpCAM immunotherapy by determining EpCAM status in the tumor biopsies is currently undergoing vigorous evaluation. However, current EpCAM antibodies are not robust enough to be able to detect EpCAM expression in all pathological tissues. Here we report a newly developed EpCAM RNA aptamer, also known as a chemical antibody, which is not only specific but also more sensitive than current antibodies for the detection of EpCAM in formalin-fixed paraffin-embedded primary breast cancers. This new aptamer, together with our previously described aptamer, showed no non-specific staining or cross-reactivity with tissues that do not express EpCAM. They were able to reliably detect target proteins in breast cancer xenograft where an anti-EpCAM antibody (323/A3) showed limited or no reactivity. Our results demonstrated a more robust detection of EpCAM using RNA aptamers over antibodies in clinical samples with chromogenic staining. This shows the potential of aptamers in the future of histopathological diagnosis and as a tool to guide targeted immunotherapy. © 2013 Shigdar et al.
Gao P.,Dalian Sixth Peoples Hospital |
Gao P.,CAS Dalian Institute of Chemical Physics |
Zhou C.,Dalian University |
Zhao L.,Dalian Sixth Peoples Hospital |
And 2 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2016
Advanced adenomas are of higher risk to progress to colorectal cancer (CRC), the third leading cause of cancerous death worldwide. Endoscopy-based adenoma removal greatly contributes to arresting the progression of adenoma to CRC. Precise diagnosis, post-polypectomy surveillance and the follow-up clinical decisions predominantly depend on histopathologic inspection of the resected tissues. The common artificial histological inspection is not fully reliable and is only compatible with the en bloc removed tissues. An alternative measure ensuring more objective tissue malignance appraisal, which is applicable to various endoscopically acquired sample types are highly appreciated. In this study, we firstly employed capillary electrophoresis-mass spectrometry-based untargeted metabolomic technique to analyze CRC and corresponding paracancerous tissues to narrow the scope of malignancy-related metabolite changes. The primary results implied the most perturbated metabolites by CRC onset were amino acids. Subsequently, a targeted amino acid analysis by ultra-performance liquid chromatography-mass spectrometry indicated 9 amino acids were of different content between advanced adenoma and CRC tissues. Finally, regression analysis of the 9 differential amino acids exhibited that methionine, tyrosine, valine and isoleucine could be used to differentiate CRC from advanced adenomas with good sensitivity and specificity (p< 0.001). Area under the receiver operating characteristic curve was 0.991. This study demonstrated the utility of metabolomic analysis in assisting malignance evaluation of colorectal neoplasia and the potential value of amino acids analysis in clinical pathology practice. © 2015 Elsevier B.V.
PubMed | University of Sydney, University of New South Wales, Dalian Sixth Peoples Hospital and Deakin University
Type: Journal Article | Journal: Cancer letters | Year: 2014
Cancer stem cells are a progressive concept to account for the cell biological nature of cancer. Despite the controversies regarding the cancer stem cell model, it has the potential to provide a foundation for new innovative treatment targeting the roots of cancer. The last two years have witnessed exceptional progress in cancer stem cell research, in particular on solid tumours, which holds promise for improved treatment outcomes. Here, we review recent advances in cancer stem cell research, discuss challenges in the field and explore future strategies and opportunities in cancer stem cell studies to overcome resistance to chemotherapy.
PubMed | Dalian Sixth Peoples Hospital and Dalian Medical University
Type: | Journal: Gastroenterology research and practice | Year: 2014
The immunoregulation between dendritic cells (DCs) and regulatory T cells (T-regs) plays an important role in the pathogenesis of ulcerative colitis (UC). Recent research showed that Fms-like tyrosine kinase 3 (Flt3) and Flt3 ligand (Flt3L) were involved in the process of DCs regulating T-regs. The DSS-induced colitis model is widely used because of its simplicity and many similarities with human UC. In this study, we observe the disease activity index (DAI) and histological scoring, detect the amounts of DCs and T-regs and expression of Flt3/Flt3L, and investigate Flt3/Flt3L participating in the process of DCs regulating T-regs in DSS-induced colitis. Our findings suggest that the reduction of Flt3 and Flt3L expression may possibly induce colonic immunoregulatory imbalance between CD103(+)MHCII(+)DCs and CD4(+)CD25(+)FoxP3(+)T-regs in DSS-induced colitis. Flt3/Flt3L participates in the process of regulating DCS and T-regs in the pathogenesis of UC, at least, in the acute stage of this disease.
PubMed | Dalian University, Dalian Sixth Peoples Hospital and CAS Dalian Institute of Chemical Physics
Type: | Journal: Journal of pharmaceutical and biomedical analysis | Year: 2015
Advanced adenomas are of higher risk to progress to colorectal cancer (CRC), the third leading cause of cancerous death worldwide. Endoscopy-based adenoma removal greatly contributes to arresting the progression of adenoma to CRC. Precise diagnosis, post-polypectomy surveillance and the follow-up clinical decisions predominantly depend on histopathologic inspection of the resected tissues. The common artificial histological inspection is not fully reliable and is only compatible with the en bloc removed tissues. An alternative measure ensuring more objective tissue malignance appraisal, which is applicable to various endoscopically acquired sample types are highly appreciated. In this study, we firstly employed capillary electrophoresis-mass spectrometry-based untargeted metabolomic technique to analyze CRC and corresponding paracancerous tissues to narrow the scope of malignancy-related metabolite changes. The primary results implied the most perturbated metabolites by CRC onset were amino acids. Subsequently, a targeted amino acid analysis by ultra-performance liquid chromatography-mass spectrometry indicated 9 amino acids were of different content between advanced adenoma and CRC tissues. Finally, regression analysis of the 9 differential amino acids exhibited that methionine, tyrosine, valine and isoleucine could be used to differentiate CRC from advanced adenomas with good sensitivity and specificity (p<0.001). Area under the receiver operating characteristic curve was 0.991. This study demonstrated the utility of metabolomic analysis in assisting malignance evaluation of colorectal neoplasia and the potential value of amino acids analysis in clinical pathology practice.