Dalian SEM Bio Engineering Technology Co.

Dalian, China

Dalian SEM Bio Engineering Technology Co.

Dalian, China
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Wang T.,Dalian University of Technology | Wang T.,Dalian SEM Bio Engineering Technology Company | Sun Y.,Liaoning Academy of Agricultural science | Jin L.,Dalian University of Technology | And 5 more authors.
Fish and Shellfish Immunology | Year: 2013

The nuclear factor κB (NF-κB) has been evolutionary conserved from insects to mammals and plays a major regulatory role in the initiation of physiological responses. In this study, we identified and characterized a primitive and functional NF-κB pathway active in the immune defence of the sea cucumber (Apostichopus japonicus). The ancient NF-κB homologues, Aj-rel and Aj-p105, share numerous signature motifs with their vertebrate orthologues, notably the Rel Homology Domain, Rel Protein Signature DNA Binding Motif, Nuclear Localization Signal and the Ankyrin Repeats for Aj-p105. Phylogenetic analyses indicate that these homologues belong to class I and II of NF-κB respectively. We examined the dimerization of Aj-rel and Aj-p105 and our results demonstrated that Aj-rel forms heterdimers with Aj-p105 and the degradation product of Aj-p105, namely Aj-p50. We further observed that LPS stimulation led to the degradation of Aj-p105 and the nuclear translocation of Aj-rel and Aj-p50. Taken together, our data indicate that the NF-κB signaling cascade is active in sea cucumber and plays a crucial role in regulating their immune defence. Our results increase the available information on sea cucumber immunity and provide new information for use in the study of the comparative and evolutionary aspects of immunity. © 2012 Elsevier Ltd.


Liu C.-G.,Shanghai JiaoTong University | Liu C.-G.,Dalian University of Technology | Liu C.-G.,Dalian SEM Bio Engineering Technology Co. | Qin J.-C.,Dalian University of Technology | And 4 more authors.
ACS Sustainable Chemistry and Engineering | Year: 2016

Lignocellulose rice straw was pretreated by three ionic liquids and instant catapult steam explosion (ICSE), and their effects on feedstock morphology, chemical composition, crystallinity index, thermostability, and glucose yield after enzymatic hydrolysis were investigated. Results showed that ionic liquid performed ideally in separation of lignocellulose components. Compared with the untreated feedstock, the addition of 1-ethyl-3-methylimidazolium acetate ([Emim]Ac) increased the glucose yield by 70.35% thanks to its marvelous solubility for cellulose and lignin. Moreover, the incorporation of ICSE further enhanced the degradation of lignocellulose. Compared to the sole utilization of ionic liquid, ICSE plus ionic liquid increased the glucose yield by 73.38% (1-butyl-3-methylimidazolium chloride, [Emim]Cl) and 74.01% (1-butyl-3-methylimidazolium chloride, [Bmim]Cl), respectively. ICSE tore up the feedstock into small and porous biomass with large specific surface area, which contributed to the superior performance of ionic liquid on rice straw dissolution. The structure changes of feedstock before and after pretreatment were observed by scanning electron microscopy, Fourier transformation infrared spectrometry, X-ray diffraction, and thermogravimetric analysis. © 2015 American Chemical Society.


Li X.,Dalian University of Technology | Wang L.,Dalian University of Technology | Zhen Y.,Dalian Medical University | Li S.,Dalian SEM Bio Engineering Technology Co. | Xu Y.,Dalian University of Technology
Journal of Animal Science and Biotechnology | Year: 2015

In recent years, the use of in-feed antibiotics for growth and disease prevention in livestock production has been under severe scrutiny. The use and misuse of in-feed antibiotics has led to problems with drug residues in animal products and increased bacterial resistance. Chicken egg yolk antibodies (IgY) have attracted considerable attention as an alternative to antibiotics to maintain swine health and performance. Oral administration of IgY possesses many advantages over mammalian IgG such as cost-effectiveness, convenience and high yield. This review presents an overview of the potential to use IgY immunotherapy for the prevention and treatment of swine diarrhea diseases and speculates on the future of IgY technology. Included are a review of the potential applications of IgY in the control of enteric infections of either bacterial or viral origin such as enterotoxigenic Escherichia coli, Salmonella spp., rotavirus, porcine transmissible gastroenteritis virus, and porcine epidemic diarrhea virus. Some potential obstacles to the adoption of IgY technology are also discussed. © 2015 Li et al.


Dai J.-Y.,Dalian University of Technology | Dai J.-Y.,Dalian SEM Bio Engineering Technology Co. | Sun Y.-Q.,Dalian University of Technology | Xiu Z.-L.,Dalian University of Technology
Engineering in Life Sciences | Year: 2014

The possibility of creating a biorefinery using inexpensive biomass has attracted a great deal of attention, which is mainly focused on the improvement of strains and fermentation, whereas few resources have been spent on downstream processing. Bio-based chemical downstream processing can become a bottleneck in industrial production because so many impurities are introduced into the fermentation broth. This review introduces a technique referred to as salting-out extraction, which is based on the partition difference between chemicals in two phases consisting of salts and polymers or hydrophilic solvents, hydrophobic solvents, and amphipathic chemicals. The effects of solvents and salts on the formation of two phases were discussed, as was the use of this method to recover bio-based chemicals. This review focused on the separation of hydrophilic chemicals (1,3-propanediol, 2,3-butanediol, acetoin, and lactic acid) from fermentation broths. Diols could be recovered at a high yield from fermentation broths without pretreatment especially with a hydrophilic solvent-based system, whereas the recovery of organic acids was slightly lower. Most of the impurities (cells and proteins) were removed during the same step. Extractive fermentations were also used for polymer-based aqueous two-phase systems. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Jin L.,Dalian University of Technology | Jin L.,Ministry of Education Center for Food Safety of Animal Origin | Li X.,Dalian University of Technology | Li X.,Ministry of Education Center for Food Safety of Animal Origin | And 5 more authors.
Aquaculture Research | Year: 2013

Egg yolk immunoglobulins (IgY) were obtained from laying hens immunized with inactivated Aeromonas hydrophila. The purified IgY was shown to inhibit the growth of A. hydrophila in vitro and the optimum concentration for inhibition of A. hydrophila-specific IgY was 75 mg mL-1. In a subsequent challenge trial, 100 carp (200~250 g) were assigned to one of ten tanks with ten carp per tank. The fish in one tank were unchallenged whereas the remaining 90 fish were injected intraperitoneally with 100 μL of A. hydrophila at a concentration of 108 cfu mL-1. For the next 21 days, all fish were moved in their respective groups to a clean tank for 20 min day-1. The fish in four tanks (one unchallenged tank and three challenged tanks) received no treatment whereas the fish in the remaining six tanks were immersed in either 0.5 g L-1 aqueous nonspecific IgY (N = 3) or 0.5 g L-1 aqueous specific IgY (N = 3). Haemoglobin concentrations, white and red blood cell numbers as well as the mortality of specific IgY-treated fish were significantly different from those of the control. © 2012 Blackwell Publishing Ltd.


Li X.,Dalian University of Technology | Li X.,Ministry of Education Center for Food Safety of Animal Origin | Wang L.,Dalian University of Technology | Wang L.,Ministry of Education Center for Food Safety of Animal Origin | And 4 more authors.
Journal of Animal Science and Biotechnology | Year: 2015

In recent years, the use of in-feed antibiotics for growth and disease prevention in livestock production has been under severe scrutiny. The use and misuse of in-feed antibiotics has led to problems with drug residues in animal products and increased bacterial resistance. Chicken egg yolk antibodies (IgY) have attracted considerable attention as an alternative to antibiotics to maintain swine health and performance. Oral administration of IgY possesses many advantages over mammalian IgG such as cost-effectiveness, convenience and high yield. This review presents an overview of the potential to use IgY immunotherapy for the prevention and treatment of swine diarrhea diseases and speculates on the future of IgY technology. Included are a review of the potential applications of IgY in the control of enteric infections of either bacterial or viral origin such as enterotoxigenic Escherichia coli, Salmonella spp., rotavirus, porcine transmissible gastroenteritis virus, and porcine epidemic diarrhea virus. Some potential obstacles to the adoption of IgY technology are also discussed. © 2015 Li et al.


Li H.,Dalian University of Technology | Jin L.,Dalian University of Technology | Wu F.,Dalian University of Technology | Thacker P.,University of Saskatchewan | And 6 more authors.
Asian-Australasian Journal of Animal Sciences | Year: 2012

Two experiments were conducted to study the effects of red pepper (Capsicum frutescens) powder or red pepper pigment on the performance and egg yolk color of laying hens. In Exp. 1, 210, thirty-wk old, Hy-line Brown laying hens were fed one of seven diets containing 0.3, 0.6, 1.2, 2.0, 4.8 or 9.6 ppm red pepper pigment or 0.3 ppm carophyll red. Each diet was fed to three replicate batteries of hens with each battery consisting of a row of five cages of hens with two hens per cage (n = 3). In Exp. 2, 180, thirty-wk old, Hyline Brown laying hens, housed similarly to those in Exp. 1, were fed an unsupplemented basal diet as well as treatments in which the basal diet was supplemented with 0.8% red pepper powder processed in a laboratory blender to an average particle size of 300 μm, 0.8% red pepper powder processed as a super fine powder with a vibrational mill (44 μm) and finally 0.8% red pepper powder processed as a super fine powder with a vibrational mill but mixed with 5% Na 2CO3 either before or after grinding. A diet supplemented with 0.3 ppm carophyll red pigment was also included (n = 3). In both experiments, hens were fed the red pepper powder or pigment for 14 days. After feeding of the powder or pigment was terminated, all hens were fed the basal diet for eight more days to determine if the dietary treatments had any residual effects. In Exp. 1, there were no differences in egg-laying performance, feed consumption or feed conversion ratio due to inclusion of red pepper pigment in the diet. Average egg weight was higher (p<0.05) for birds fed 1.2, 2.4 or 9.6 ppm red pepper pigment than for birds fed the diet containing 0.3 ppm red pepper pigment. On d 14, egg color scores increased linearly as the level of red pepper pigment in the diet increased. In Exp. 2, feeding red pepper powder did not affect egg-laying performance, feed consumption or feed conversion ratio (p>0.05). However, compared with the control group, supplementation with all of the red pepper powder treatments increased egg weight (p<0.05). All the red pepper powder treatments also increased (p<0.05) the yolk color score compared with the control. The results of the present study suggest that both red pepper powder and pigment are effective feed additives for improving egg yolk color for laying hens. Copyright © 2012 by Asian-Australasian Journal of Animal Sciences.


PubMed | Dalian SEM Bio Engineering Technology Co. and Dalian University of Technology
Type: | Journal: Evidence-based complementary and alternative medicine : eCAM | Year: 2015

Camellia euphlebia Merr. ex Sealy is a traditional Chinese medicine that has been widely used for improvement of human emotions in the Guangxi Province of southern China. However, there are no studies about the anxiolytic and antidepressant activities of Camellia euphlebia. This study evaluated the anxiolytic and antidepressant activities of the aqueous extract from Camellia euphlebia (CEE) in mice. We found that administration of 400mg/kg CEE or 20mg/kg fluoxetine for 7 days significantly reduced the immobility time in both TST and FST. Oral administration of 100mg/kg extract or 4mg/kg diazepam for 7 days significantly increased the percentage of time spent and the number of entries into the open arms of the EPMT. In addition, the time spent by mice in the illuminated side of the LDBT was increased. Furthermore, pretreatment with 400mg/kg CEE for 7 days significantly elevated the level of 5-HT and DA in the whole brain of mice. These results provide support for the potential anxiolytic and antidepressant activity of Camellia euphlebia and contribute towards validation of the traditional use of Camellia euphlebia in the treatment of emotional disorders.


PubMed | Yunnan Agricultural University, Dalian SEM Bio Engineering Technology Co. and Dalian University of Technology
Type: Journal Article | Journal: Journal of animal science and biotechnology | Year: 2015

In recent years, the development of antibiotic resistant bacteria has become a global concern which has prompted research into the development of alternative disease control strategies for the swine industry. Bacteriophages (viruses that infect bacteria) offer the prospect of a sustainable alternative approach against bacterial pathogens with the flexibility of being applied therapeutically or for biological control purposes. This paper reviews the use of phages as an antimicrobial strategy for controlling critical pathogens including Salmonella and Escherichia coli with an emphasis on the application of phages for improving performance and nutrient digestibility in swine operations as well as in controlling zoonotic human diseases by reducing the bacterial load spread from pork products to humans through the meat.


PubMed | Dalian SEM Bio Engineering Technology Co., Dalian Medical University and Dalian University of Technology
Type: Journal Article | Journal: Journal of animal science and biotechnology | Year: 2015

In recent years, the use of in-feed antibiotics for growth and disease prevention in livestock production has been under severe scrutiny. The use and misuse of in-feed antibiotics has led to problems with drug residues in animal products and increased bacterial resistance. Chicken egg yolk antibodies (IgY) have attracted considerable attention as an alternative to antibiotics to maintain swine health and performance. Oral administration of IgY possesses many advantages over mammalian IgG such as cost-effectiveness, convenience and high yield. This review presents an overview of the potential to use IgY immunotherapy for the prevention and treatment of swine diarrhea diseases and speculates on the future of IgY technology. Included are a review of the potential applications of IgY in the control of enteric infections of either bacterial or viral origin such as enterotoxigenic Escherichia coli, Salmonella spp., rotavirus, porcine transmissible gastroenteritis virus, and porcine epidemic diarrhea virus. Some potential obstacles to the adoption of IgY technology are also discussed.

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