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Bao W.,International Peace Maternity and Child Health Hospital of China Welfare Institute | Yu X.-H.,Dalian Obstetrics and Gynecology Hospital | Wan X.-P.,International Peace Maternity and Child Health Hospital of China Welfare Institute
Journal of Shanghai Jiaotong University (Medical Science) | Year: 2012

Neurotrophin-tyrosine kinase receptor (NT-Trk) signaling pathway plays an important role in the neural regulation of development. Now it has been recently revealed that Trk may also participate in the pathogenesis of many malignant tumors. It is demonstrated that the expression of TrkB is related to oncogenesis. The activation of brain-derived neurotrophic factor (BDNF)/TrkB signal transduction pathway stimulates tumor cell survival and angiogenesis, and contributes to the resistance to cytotoxic drugs and anoikis. The role of TrkB signal transduction pathway in tumors is reviewed in this paper. Source

Wang Y.-S.,Fudan University | Cao R.,Dalian Obstetrics and Gynecology Hospital | Jin H.,Fudan University | Huang Y.-P.,Fudan University | And 5 more authors.
Journal of Hematology and Oncology | Year: 2011

Background: Endometrial carcinoma is one of the most common gynecological malignancies in women. The diagnosis of the disease at early or premalignant stages is crucial for the patient's prognosis. To date, diagnosis and follow-up of endometrial carcinoma and hyperplasia require invasive procedures. Therefore, there is considerable demand for the identification of biomarkers to allow non-invasive detection of these conditions. Methods. In this study, we performed a quantitative proteomics analysis on serum samples from simple endometrial hyperplasia, complex endometrial hyperplasia, atypical endometrial hyperplasia, and endometrial carcinoma patients, as well as healthy women. Serum samples were first depleted of high-abundance proteins, labeled with isobaric tags (iTRAQ), and then analyzed via two-dimensional liquid chromatography and tandem mass spectrometry. Protein identification and quantitation information were acquired by comparing the mass spectrometry data against the International Protein Index Database using ProteinPilot software. Bioinformatics annotation of identified proteins was performed by searching against the PANTHER database. Results: In total, 74 proteins were identified and quantified in serum samples from endometrial lesion patients and healthy women. Using a 1.6-fold change as the benchmark, 12 proteins showed significantly altered expression levels in at least one disease group compared with healthy women. Among them, 7 proteins were found, for the first time, to be differentially expressed in atypical endometrial hyperplasia. These proteins are orosomucoid 1, haptoglobin, SERPINC 1, alpha-1-antichymotrypsin, apolipoprotein A-IV, inter-alpha-trypsin inhibitor heavy chain H4, and histidine-rich glycoprotein. Conclusions: The differentially expressed proteins we discovered in this study may serve as biomarkers in the diagnosis and follow-up of endometrial hyperplasia and endometrial carcinoma. © 2011 Wang et al; licensee BioMed Central Ltd. Source

Zhang M.,Dalian Obstetrics and Gynecology Hospital
Journal of Dalian Medical University | Year: 2012

[Objective] To detect expresson of interleukin-18 (IL-18) in the tissne with premature rupture of fetal membrane (PROM) and clinical pathological significance of the expression. [Methods] The expression of IL-18 was detected in 42 tissues with premature rupture of fetal membranes and 28 normal of fetal membrane tissues without by immunohistochemistry. [Results] The expression of IL-18 was significantly higher in premature rupture of membrane than that in normal (P < 0.05), and the expression of the tissues in PROM with sub-clinical chorioamonitis was significantly higher than that without (P < 0.05). The correlativity between the expression of IL-8 and the time of PROM was not clear (P > 0.05). [Conclusions] The IL-18 could become a predictable index of PROM. Source

Han L.,Dalian Obstetrics and Gynecology Hospital | Wu J.-L.,Dalian Blood Center | Yang L.-X.,Maternal and Child Health Hospital
Wspolczesna Onkologia | Year: 2012

Aim of the study: To evaluate the effect of combined use of rapamycin and cisplatin against Hela cells in vitro. Material and methods: The inhibitory effects of rapamycin and cisplatin, used alone or combination, on the proliferation of Hela cell were measured with MTT assay and median-effect plot analysis. Results: Combined use of rapamycin and cisplatin significantly improved the chemotherapeutic effect against Hela cells. The inhibitory rates were dose-dependent. Rapamycin and cisplatin showed synergistic effects in the chemotherapy of Hela cells (q > 1.15, King's formula). Conclusions: Combined use of rapamycin and cisplatin significantly improves the chemotherapeutic effect against Hela cells. Source

Li C.,Liaoning Medical University | Shan Z.,Liaoning Medical University | Mao J.,Liaoning Medical University | Wang W.,Liaoning Medical University | And 13 more authors.
Journal of Clinical Endocrinology and Metabolism | Year: 2014

Context: Guidelines of the American Thyroid Association (ATA) proposed that the upper limit of the TSH reference range should be 2.5 mIU/L in first trimester, but the reported ranges in China are significantly higher. Objective: Our objective was to establish a rational reference range of serum TSH for diagnosis of subclinical hypothyroidism in the first trimester of pregnant women in China. Design: We screened 4800 pregnant women in the first trimester and 2000 women who planned to become pregnant and evaluated 535 pregnant women in follow-up visits during the second and third trimester. Results: Median concentrations of serum TSH decreased significantly from the seventh week of gestation. The median of TSH from 4 to 6 weeks was significantly higher than from 7 to 12 weeks (2.15 [0.56-5.31] mIU/L vs 1.47 [0.10-4.34] mIU/L, P < .001); however, there was no significant difference compared with nonpregnant women (2.07 [0.69-5.64] mIU/L; P < .784). The median of free T4 was not significantly altered in the first trimester. The prevalence of subclinical hypothyroidism in the 4800 pregnant women was 27.8% on the diagnostic criteria of TSH >2.5 mIU/L and 4.0% using the reference interval derived by our laboratory (0.14-4.87 mIU/L). Additionally, of 118 pregnant women who had serum TSH >2.5 mIU/L in the first trimester, only 30.0% and 20.3% of them at the 20th and 30th week of gestation had TSH >3.0 mIU/L. Conclusions: The reference range for nonpregnant women can be used for the assessment of pregnant women at 4 to 6 weeks of gestation. The upper limit of serum TSH in the first trimester was much higher than 2.5 mIU/L in Chinese pregnant women. (J Clin Endocrinol Metab 99: 73-79, 2014). © Copyright 2014 by The Endocrine Society. Source

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