Dalian Obstetrics and Gynecology Hospital
Dalian Obstetrics and Gynecology Hospital
Li T.,Capital Medical University |
Li T.,Dalian Medical University |
Zhang J.,Dalian Obstetrics and Gynecology Hospital |
Liu R.-Y.,Dalian Medical University |
And 5 more authors.
Neuroscience Letters | Year: 2013
Previous studies have demonstrated that the sonic hedgehog (Shh) pathway plays a neuro-protective role. However, whether the Shh pathway is induced by subarachnoid hemorrhage (SAH) has not been investigated. We sought to investigate Shh activation in the cortex in the early stage of SAH, and assessed the effect of cyclopamine (a specific inhibitor of the Shh pathway) on Shh pathway regulation and evaluated the impact of cyclopamine on SAH. We found that the Shh pathway was up-regulated in the cortex after SAH, and that blocking the Shh pathway increased cell apoptosis. Early brain damages, including brain edema, blood-brain barrier impairment, and cortical apoptosis were significantly aggravated following with cyclopamine treatment compared with vehicle treatment. Our results suggest that the Shh pathway should be activated in the brain after SAH, and plays a beneficial role in SAH development, possibly by inhibiting cerebral oxidative stress through induction of antioxidant and detoxifying enzymes. © 2013 Elsevier Ireland Ltd.
Zhao S.-Y.,Peking University |
Zhao S.-Y.,Affiliated Hospital Guiyang Medical College |
Qiao J.,Peking University |
Chen Y.-J.,Peking University |
And 4 more authors.
Fertility and Sterility | Year: 2010
Objective: To evaluate the effect of growth differentiation factor-9 (GDF-9) and bone morphogenetic protein-15 (BMP-15) on the development of follicles among patients with polycystic ovary syndrome (PCOS). Design: Case-control study. Setting: University Hospital. Patient(s): Twenty-two oocytes were obtained from 15 patients with PCOS and 67 oocytes from 58 controls. Cumulus granulosa cells (GC) were obtained from 16 patients with PCOS and controls treated with intracytoplasmic sperm injection. Intervention(s): Immunofluorescence combined with laser scanning confocal microscopy and immunocytochemistry were used to analyze the expression of GDF-9 and BMP-15 in oocytes and cumulus GCs. Main Outcome Measure(s): To detect the protein expression levels. Result(s): No significant difference was found in the expression of GDF-9 and BMP-15 in the oocytes and BMP-15 in the cumulus GCs of patients with PCOS and controls. However, the expression of GDF-9 in cumulus GCs of patients with PCOS was decreased significantly compared with controls (8.88 ± 1.52 vs. 5.01 ± 0.83). Conclusion(s): The expression of GDF-9 and BMP-15 in the oocytes of patients with PCOS who received ovulation induction treatment was in the normal range, but the GDF-9 expression in cumulus GCs from patients with PCOS was significantly lower than the normal. Reduced GDF-9 expression in cumulus GCs of patients with PCOS appears to be associated with decreased long-term developmental potential of the oocytes of patients with PCOS. © 2010 American Society for Reproductive Medicine.
PubMed | Dalian Obstetrics and Gynecology Hospital, Shenyang Womens and Childrens Hospital, Dalian Medical University, 11 Health and 5 more.
Type: | Journal: Thyroid : official journal of the American Thyroid Association | Year: 2016
A self-sequential longitudinal reference interval may be expected to minimize the inter-individual variation of thyroid function. Comparison between the self-sequential longitudinal reference interval (SLRI) and cross-sectional reference interval (CSRI) in pregnancy has not been well investigated. The objectives of this study were to establish a stringent SLRI of thyroid function in pregnant women and to compare it with the conventional CSRI.Three cohorts were enrolled: group 1, pregnant women for an SLRI (n=99); group 2, pregnant women for a CSRI (n=1318); group 3, non-pregnant control women (NC) as a control group (n=301) according to the criteria of the National Academy of Clinical Biochemistry. Thyrotropin (TSH), total thyroxine (TT4), free thyroxine (fT4), total triiodothyronine (TT3), free triiodothyronine (fT3), serum ferritin (SF), and urine iodine concentration (UIC) were measured in the three groups.Compared with CSRI, the reference interval of the SLRI group had narrower reference intervals of fT4 in the first and second trimesters (p<0.05). The median of TSH was at a low level during the first trimester, and then gradually elevated in the second and third trimesters. The median of fT4 persistently decreased from 12 weeks, and did not return to the level of the NC group until 12 months postpartum. The TT4 increased to 131.4nmol/L at gestational week 8, and reached a peak (170.0nmol/L) at gestational week 12. In the first trimester, the prevalence of hypothyroxinemia was 9.1%, 4.0%, and 2.0% with a fT4 value below the 10th, 5th, and 2.5th percentile, respectively. In contrast, 29.3% of TT4 values were below the lower non-pregnancy reference limit multiplied by 1.5.No significant difference was found between a SLRI and a CSRI, even in a stringent self-sequential longitudinal reference interval of thyroid function in pregnant women. In addition, the limit of TT4 below the non-pregnant level multiplied by a factor 1.5 is not appropriate for diagnosing hypothyroxinemia in the first trimester.
Han L.,Dalian Obstetrics and Gynecology Hospital |
Wu J.-L.,Dalian Blood Center |
Yang L.-X.,Maternal and Child Health Hospital
Wspolczesna Onkologia | Year: 2012
Aim of the study: To evaluate the effect of combined use of rapamycin and cisplatin against Hela cells in vitro. Material and methods: The inhibitory effects of rapamycin and cisplatin, used alone or combination, on the proliferation of Hela cell were measured with MTT assay and median-effect plot analysis. Results: Combined use of rapamycin and cisplatin significantly improved the chemotherapeutic effect against Hela cells. The inhibitory rates were dose-dependent. Rapamycin and cisplatin showed synergistic effects in the chemotherapy of Hela cells (q > 1.15, King's formula). Conclusions: Combined use of rapamycin and cisplatin significantly improves the chemotherapeutic effect against Hela cells.
Ji W.,Shenyang University |
Ji W.,Dalian Obstetrics and Gynecology Hospital |
Fu J.,Shenyang University |
Nie H.,China Pharmaceutical University |
Xue X.,Shenyang University
Pediatrics International | Year: 2012
Background: The aim of the present study was to investigate the expression and activity of epithelial sodium channel (ENaC) in hyperoxia-induced bronchopulmonary dysplasia (BPD) in neonatal rats. Methods: Neonatal rats were exposed to hyperoxia to establish BPD models (control group was exposed to air), lung water was measured and Western blot was applied to detect the expression of three homologous subunits: α-, β- and γ-ENaC in the lung tissues. Furthermore, ATII cells were isolated from neonatal rats, and primarily cultured under normoxic or hyperoxic conditions. The ENaC expression was also examined in these cells. In addition, the amiloride-sensitive Na+ currents induced by hyperoxia were recorded using the whole-cell patch clamp technique. Results: The α-ENaC expression was increased after 5 days of hyperoxia in rat lung tissues, whereas not after 1, 3 and 7 days. ATII cells showed α-ENaC expression was reduced after 1 and 2 days' hyperoxia, but no change after 3 days. In contrast, β- and γ-ENaC expression was increased after hyperoxia in both in vivo and in vitro experiments. The amiloride-sensitive Na+ currents in hyperoxia-exposed ATII cells were also increased, which was consistent with the upregulated expression of β- and γ-ENaC. Conclusion: Hyperoxia upregulates the expression of ENaC, especially β- and γ-ENaC subunits, in both neonatal rat lung tissues and ATII cells. Hyperoxia also enhanced the activity of ENaC in neonatal rat ATII cells. Dysfunctional transport of Na+ may not be a key factor involving pulmonary edema at the early stage of BPD. © 2012 The Authors.
Li C.,Liaoning Medical University |
Shan Z.,Liaoning Medical University |
Mao J.,Liaoning Medical University |
Wang W.,Liaoning Medical University |
And 13 more authors.
Journal of Clinical Endocrinology and Metabolism | Year: 2014
Context: Guidelines of the American Thyroid Association (ATA) proposed that the upper limit of the TSH reference range should be 2.5 mIU/L in first trimester, but the reported ranges in China are significantly higher. Objective: Our objective was to establish a rational reference range of serum TSH for diagnosis of subclinical hypothyroidism in the first trimester of pregnant women in China. Design: We screened 4800 pregnant women in the first trimester and 2000 women who planned to become pregnant and evaluated 535 pregnant women in follow-up visits during the second and third trimester. Results: Median concentrations of serum TSH decreased significantly from the seventh week of gestation. The median of TSH from 4 to 6 weeks was significantly higher than from 7 to 12 weeks (2.15 [0.56-5.31] mIU/L vs 1.47 [0.10-4.34] mIU/L, P < .001); however, there was no significant difference compared with nonpregnant women (2.07 [0.69-5.64] mIU/L; P < .784). The median of free T4 was not significantly altered in the first trimester. The prevalence of subclinical hypothyroidism in the 4800 pregnant women was 27.8% on the diagnostic criteria of TSH >2.5 mIU/L and 4.0% using the reference interval derived by our laboratory (0.14-4.87 mIU/L). Additionally, of 118 pregnant women who had serum TSH >2.5 mIU/L in the first trimester, only 30.0% and 20.3% of them at the 20th and 30th week of gestation had TSH >3.0 mIU/L. Conclusions: The reference range for nonpregnant women can be used for the assessment of pregnant women at 4 to 6 weeks of gestation. The upper limit of serum TSH in the first trimester was much higher than 2.5 mIU/L in Chinese pregnant women. (J Clin Endocrinol Metab 99: 73-79, 2014). © Copyright 2014 by The Endocrine Society.
PubMed | Dalian Obstetrics and Gynecology Hospital, Shenyang Women and Children Health Care Center, The First Hospital of Dandong, No 202 Hospital Of Peoples Liberation Army and 3 more.
Type: | Journal: BioMed research international | Year: 2015
Maternal thyroid dysfunction in early pregnancy may increase the risk of adverse pregnancy complications and neurocognitive deficiencies in the developing fetus. Currently, some researchers demonstrated that body mass index (BMI) is associated with thyroid function in nonpregnant population. Hence, the American Thyroid Association recommended screening thyroid function in obese pregnant women; however, the evidence for this is weak. For this purpose, our study investigated the relationship between high BMI and thyroid functions during early pregnancy in Liaoning province, an iodine-sufficient region of China.Serum thyroid stimulating hormone (TSH), free thyroxine (FT4), thyroid-peroxidase antibody (TPOAb), thyroglobulin antibody (TgAb) concentration, urinary iodine concentration (UIC), and BMI were determined in 6303 pregnant women.BMI 25 kg/m(2) may act as an indicator of hypothyroxinemia and TPOAb positivity and BMI 30 kg/m(2) was associated with increases in the odds of hypothyroidism, hypothyroxinemia, and TPOAb positivity. The prevalence of isolated hypothyroxinemia increased among pregnant women with BMI > 24 kg/m(2).High BMI during early pregnancy may be an indicator of maternal thyroid dysfunction; for Asian women whose BMI > 24 kg/m(2) and who are within 8 weeks of pregnancy, thyroid functions should be assessed especially.
Fan Y.,Dalian Institute of Blood Transfusion |
Yu W.,Dalian Institute of Blood Transfusion |
Ye P.,Dalian Institute of Blood Transfusion |
Wang H.,Dalian Obstetrics and Gynecology Hospital |
And 5 more authors.
DNA and Cell Biology | Year: 2011
Ovarian cancer is the leading cause of death among all gynecological cancers. This is mainly attributed to its frequent presentation at an advanced stage (International Federation of Gynecology and Obstetrics stage III-IV). Nuclear factor-kappaB (NF-κB) is critically involved in the carcinogenesis and development of ovarian cancer. A functional insertion/deletion polymorphism (-94 ins/del ATTG) in the promoter region of the NFKB1 gene, which encodes the p50 subunit of the NF-κB protein, has been recently identified and shown to increase the susceptibility to many diseases. The purpose of this study was to explore the association between this polymorphism and the risk of advanced ovarian cancer in a Chinese population. A total of 179 advanced ovarian cancer patients and 223 healthy controls were recruited into this study. Genotypes were determined using polymerase chain reaction-capillary electrophoresis method. The insertion increased the risk of advanced ovarian cancer (odds ratio = 2.111, 95% confidence intervals = 1.125-3.961, p = 0.019 for heterozygote insertion, and odds ratio = 2.656, 95% confidence intervals = 1.397-5.051, p = 0.002 for homozygote insertion) compared with homozygote deletion. Similar results were seen in age-adjusted analyses (p < 0.05). Our preliminary results suggest that NFKB1-94 ins/del ATTG promoter polymorphism may be associated with increased susceptibility to advanced ovarian cancer. © Copyright 2011, Mary Ann Liebert, Inc.
Zhang M.,Dalian Obstetrics and Gynecology Hospital
Journal of Dalian Medical University | Year: 2012
[Objective] To detect expresson of interleukin-18 (IL-18) in the tissne with premature rupture of fetal membrane (PROM) and clinical pathological significance of the expression. [Methods] The expression of IL-18 was detected in 42 tissues with premature rupture of fetal membranes and 28 normal of fetal membrane tissues without by immunohistochemistry. [Results] The expression of IL-18 was significantly higher in premature rupture of membrane than that in normal (P < 0.05), and the expression of the tissues in PROM with sub-clinical chorioamonitis was significantly higher than that without (P < 0.05). The correlativity between the expression of IL-8 and the time of PROM was not clear (P > 0.05). [Conclusions] The IL-18 could become a predictable index of PROM.
Zhang J.,Dalian Obstetrics and Gynecology Hospital
Zhonghua nan ke xue = National journal of andrology | Year: 2012
To study the correlation of nucleotide polymorphism in the ubiquitin-specific protease 26 (Usp26) gene with idiopathic male infertility and its action mechanism in spermatogenesis. Based on the WHO criteria (4th ed.), we selected 41 patients with idiopathic infertility from 150 infertile males, and enlisted 50 normal fertile men as controls. We examined the selected patients for mutations using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis, and determined how and where the mutations occurred by gene sequencing. Low sperm concentration and poor sperm motility were found in the 41 men with idiopathic infertility. Nine (22.0%) of them exhibited changes in the Usp26 gene (P = 0.01), including compound mutations of 364insACA and 460G > A in 8 (19.5%, P = 0.01) and 1 044T > A substitution in 1 (2.4%, P > 0.05). The above three variations led to changes in the coding amino acids. No other changes were found in the remaining patients and normal fertile controls. The nucleotide polymorphisms of the Usp26 gene might be closely related with idiopathic male infertility, and exert negative effect on the testis function.