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Jiang S.-Y.,Dalian University of Technology | Jiang S.-Y.,Dalian Institute for Food and Drug Control | Jiao J.,Dalian Institute for Food and Drug Control | Lu J.,Dalian Institute for Food and Drug Control | And 2 more authors.
Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis | Year: 2013

To develop a spectrophotometric method for determining the concentration of recombinant hirudin (rH) in urine of rats. rH concentration was determined based on the rH inhibility to thrombin which hydrolyzed the Chromozym TH TH chromogenic substrate to form the specific pNA absorbed at 405nm. The standard rH in rat urine was determined by the spectrophotometric method at concentration of 6.25 to 75 ng·mL-1 with day and intra-day RSD <10%, method recoveries of >95% and the dilution recoveries of >93%. The rH samples of rat urines which iv dose of 0.5, 1.0, and 2.0 mg·kg-1 were collected and analyzed by the CSA method. Their cumulative excretion rH at 0~12 hr were (116.850±57.160), (235.544±39.375) and (474.986±85.426) μg·kg-1. The calculated cumulative excretion rate of three doses is about 23% which indicates that the rH was eliminated in the way of a linear kinetics in rats. The rH content in rat urine could be measured by the spectrophotometric method accurately, reliably and sensitively for the rH urinary excretion dynamics study.

Lin L.,Dalian Institute for Food and Drug Control | Lin L.,Dalian Medical University | Jiang S.-Y.,Dalian Institute for Food and Drug Control | Tang X.-Q.,Dalian Medical University
Journal of Dalian Medical University | Year: 2014

Peptide drugs have been widely used for the low cost, high biological activity, low side effects, no accumulation of toxic, target specificity and other s. However, the natural peptide drugs have low concentration and low purity; can not meet the requirement of clinical applications. Synthetic peptide drugs have the priority of short synthetic cycle and large production volume, which are important for the promotion of clinical application of peptide drugs. Consulting the relevant literatures, this article intends to review the drugs synthesis and provide a reference for future drug development and clinical application.

Li X.,Dalian University of Technology | Wang W.,Dalian University of Technology | Dou J.,Dalian Institute for Food and Drug Control | Gao J.,Dalian University of Technology | And 3 more authors.
Journal of Water Process Engineering | Year: 2015

Antibiotics have been considered to be a potential risk to human and ecological health. In an effort to develop convenient and effective treatment technology for the removal of fluoroquinoline antibiotics ciprofloxacin (CIP) from aqueous solution, a dynamic adsorption experimental apparatus based on carbon nanofibers (CNFs) was built and the adsorption of CIP was quantitatively measured by in situ fluorescence (FL) for the first time. The experimental results show that the adsorption kinetics and isotherms match well with the pseudo-second-order model and the Langmuir model, respectively. The adsorption equilibrium accomplished within 4h, which is much shorter than the adsorption of CIP on the other carbon materials reported before. The maximum adsorption capacity (q m) is 10.36mg/g, which is similar to the q m of CIP adsorption on biocomposite fibers. The strong adsorption affinity exhibited by CNFs was ascribed to the contributions of π-π electron-donor-acceptor (EDA) interaction, hydrophobic interaction and electrostatic interaction. The dynamic adsorption of CIP on CNFs provides a novel and effective approach for CIP removal, implying the potential practical application of CNFs in the field of water treatment. © 2015.

Jiang S.-Y.,Dalian University of Technology | Jiang S.-Y.,Dalian Institute for Food and Drug Control | Jiao J.,Dalian Institute for Food and Drug Control | Zhang T.-T.,Dalian Institute for Food and Drug Control | Xu Y.-P.,Dalian University of Technology
PLoS ONE | Year: 2013

Aim:To compare the analytical methods used to study the pharmacokinetics of recombinant hirudin in the plasma of rats that had been injected with 125I-recombinant hirudin.Methods:2.0 mg/kg 125I-recombinant hirudin were injected intravenously into rats. The recombinant hirudins in the plasma was analyzed by chromogenic substrate assay, enzyme-linked immunosorbent assay (ELISA), total radioisotope assay (RA) and trichloroacetic acid pre-treated total radioisotope assay (TCA-RA).Results:The chromogenic substrate assay standard curve was linear over the concentration range from 3.12 to 40.00 ng/ml for the recombinant hirudin in plasma. The relative standard deviations (RSD) for the intra- and inter-day variation were 5.0 to 6.3% and 11.9 to 12.6%, respectively. The recoveries of recombinant hirudin was 89.8% to 100.7%. The limit of quantification (LOQ) was 3.12 ng/ml. The concentration-time curve of the recombinant hirudin in the plasma could be explained as a two-compartment model. Pharmacokinetic parameters, including the half-life of distribution phase (t1/2 α), the half-life of elimination phase (t1/2 β), volume of apparent distribution (Vd), and area under the concentration-time curve from zero to infinite time (AUC0-t) were 7.59 min, 46.99 min, 0.17 L/kg, and 204.5 mg/L/min, respectively, as determined by chromogenic substrate assay; 6.41 min, 47.28 min, 1.24 L/kg, and 575.18 mg/L/min, respectively, as determined by ELISA; 3.69 min, 701.90 min, 0.04 L/kg, and 4189 mg/L/min, respectively as determined by RA; and 4.57 min, 724.9 min, 0.09 L/kg, and 2329 mg/L/min, respectively, as determined by TCA-RA.Conclusions:The chromogenic substrate assay on the concentration dynamics of the recombinant hirudin in the plasma is a specific, sensitive, and accurate analytical method for pharmacokinetic studies. Moreover, the pharmacokinetic parameters determined by the chromogenic substrate assay and ELISA are congruent except for AUC. © 2013 Jiang et al.

Wang Y.-Q.,Dalian University of Technology | Wang S.-S.,Dalian University of Technology | Zhu J.,Dalian University of Technology | Wang L.,Dalian Institute for Food and Drug Control | And 2 more authors.
Journal of Food and Drug Analysis | Year: 2016

The objective of this study is to establish a centrifugal partition chromatography (CPC) method for determination of the urea ingredient in urea cream. The mechanism of this method is that urea is determined by UV detector at 430 nm after being extracted from the cream and derivatized on line via Ehrlich reaction in rotor of CPC, where the reaction products dissolve in the mobile phase and the cream matrix retains in the stationary phase. The mixed solvent consisting of n-hexane, methanol, hydrochloric acid and p-dimethylaminobenzaldehyde with a ratio of 1000 mL:1000 mL:18 mL:2.0 g is used for solvent system of CPC. The CPC method proposed offers good precision and convenience without complex sample pretreatment processes. © 2015.

Liao W.,Xiamen University of Technology | Xu S.,Xiamen University of Technology | Wang L.,Xiamen University of Technology | Yan B.,Xiamen University of Technology | Song Y.,Dalian Institute for Food and Drug Control
Chinese Journal of Environmental Engineering | Year: 2014

The degradation pattern of typical endocrine disruptor atrazine in microwave-assisted photocatalytic process was studied using a circulating fluidized bed photo-reactor. According to the apparent kinetics study, the degradation of atrazine in microwave-assisted photocatalytic process obeyed the apparent first order kinetics when the initial concentration of atrazine was low. The reaction rate constant showed a negative linear correlation with the initial concentration of atrazine, while it was linearly related to the ultraviolet intensity and parabolic related to the catalyst dosage. Furthermore, the apparent reaction rate constant followed the equation of kobs=3.95×10-4 c0 -0.2703I1.2224W0.3283 after linear regression fitting of experimental data. The calculated value from this model almost agreed with the experimental result, with a mean standard deviation of 0.5%. Therefore, the kinetics model could be used to predict the degradation pattern of organic pollutant of low concentration in microwave-assisted photocatalytic process.

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