Dalian Huixin Titanium Equipment Dev. Co.

Ganjingzi District, China

Dalian Huixin Titanium Equipment Dev. Co.

Ganjingzi District, China
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Ma Y.,Dalian Ocean University | Liu Z.,Dalian Ocean University | Yang Z.,Dalian Huixin Titanium Equipment Dev. Co. | Li M.,Dalian Ocean University | And 2 more authors.
Fish and Shellfish Immunology | Year: 2013

A feeding experiment was conducted to determine effects of Hanseniaspora opuntiae C21 on immune response and disease resistance against Vibrio splendidus infection in juvenile sea cucumbers Apostichopus japonicus. Sea cucumbers were fed with either diets containing C21 at 104, 105 and 106 CFU g-1 feed or a control diet for 30-50 days, respectively. After feeding for 30 days and 45 days, five sea cucumbers from each tank were sampled for immunological analyses. Results indicated that C21 significantly improved the phagocytic activity in coelomocytes of sea cucumbers (P < 0.05). Moreover, C21 administration significantly enhanced lysozyme (LSZ), phenoloxidase activity (PO), total nitric oxide synthase (T-NOS), superoxide dismutase (SOD), alkaline phosphatase (AKP) and acid phosphatase (ACP) activities in coelomic fluid, and LSZ, T-NOS, AKP and ACP activities in coelomocytes lysate supernatant (CLS) of sea cucumbers (P < 0.05). After feeding for 45 days, 10 sea cucumbers from each dose group were challenged with V. splendidus NB13. Cumulative incidence and mortality of sea cucumbers fed with C21 were found to be lower than those of control group. After feeding for 50 days, sea cucumbers in 104 CFU g-1 C21 treatment and control tanks were subjected to acute salinity changes (from 30 to 20) for 24 h in the laboratory, and the immunological parameters were measured to evaluate the immune capacities of the A. japonicus. Phagocytic, LAZ and T-NOS activities of C21-treated group were higher than those of control group, indicating that salinity stress tolerance of sea cucumber was enhanced by C21. The present results showed that a diet supplemented with C21 could stimulate the immune system of juvenile A. japonicus thus enhancing their resistance against V. splendidus. © 2012 Elsevier Ltd.


Yang Z.P.,Dalian Huixin Titanium Equipment Dev. Co. | Sun J.M.,Dalian Huixin Titanium Equipment Dev. Co. | Xu Z.,Dalian Huixin Titanium Equipment Dev. Co. | Zhang C.C.,Dalian Huixin Titanium Equipment Dev. Co. | Zhou Q.,Dalian Huixin Titanium Equipment Dev. Co.
Animal Feed Science and Technology | Year: 2014

We investigated the effects of dietary administration Metschnikowia sp. C14 on growth and intestinal digestive enzymes of juvenile sea cucumber Apostichopus japonicus. Sea cucumbers were fed with diets containing C14 at 0 (control), 104, 105 and 106CFU/g feed, respectively. After feeding for 45 days, the specific growth rate was higher in sea cucumbers fed C14-supplemented diets at 104 and 105CFU/g feed than in the controls (P<0.05). The intestinal trypsin and lipase activities in sea cucumbers were significantly enhanced by C14 administration at 104, 105 and 106CFU/g feed compared to those of the controls (P<0.05). After feeding for 23-42 days, the presence of C14 was evidenced in the intestinal community of sea cucumbers by denaturing gradient gel electrophoresis (DGGE). The intestinal fungal communities of sea cucumbers fed C14-supplemented diets were different from that of the control on day 42. Additionally, sea cucumbers were switched to the control diet from day 16 to day 46. As confirmed by DGGE, C11 colonization of the intestine could be detected until day 46. The present study demonstrates that C14 is able to successfully colonize the intestine through dietary supplementation, and that this colonization improves the growth and intestinal digestive enzymes of juvenile A. japonicus. © 2014 Elsevier B.V.


Liu Z.-M.,Dalian Ocean University | Ma Y.-X.,Dalian Ocean University | Yang Z.-P.,Dalian Huixin Titanium Equipment Dev. Co. | Li M.,Dalian Ocean University | And 2 more authors.
Aquaculture | Year: 2012

Metschnikowia sp. C14 was isolated from the intestine of sea cucumber Apostichopus japonicus. It inhibited the growth of the pathogenic Shewanella marisflavi AP629 and Vibrio splendidus NB13 of A. japonicus in vitro. The yeast was added to basal diet at 10 4, 10 5 and 10 6CFUg -1 of feed to evaluate its effects on immune response and disease resistance against V. splendidus infection in juvenile sea cucumber A. japonicus. After feeding for 30days and 45days, five sea cucumbers from each tank were sampled for immunological analyses. Results indicated that C14 significantly improved the phagocytic activity in coelomocytes of sea cucumbers (P<0.05). Moreover, C14 administration significantly elevated lysozyme (LSZ), phenoloxidase activity (PO), total nitric oxide synthase (T-NOS), superoxide dismutase (SOD), alkaline phosphatase (AKP) in coelomic fluid, and LSZ, T-NOS, SOD and acid phosphatase (ACP) activities in CLS of sea cucumbers (P<0.05). After feeding for 45days, ten sea cucumbers from each dose group were challenged with V. splendidus NB13. Cumulative incidence and mortality of sea cucumbers fed with C14 were found to be lower than those of control group. The present results show that diet supplemented with C14 stimulates the immune system of juvenile A. japonicus thus enhancing their resistance against V. splendidus. C14 enhanced the immune responses and disease resistance of sea cucumbers. © 2012 Elsevier B.V.

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