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Dapeng S.,Dalian Fusheng Natural Medicinal Development Co. Ltd | Dapeng S.,Liaoning Medical University | Mingming L.,Dalian Fusheng Natural Medicinal Development Co. Ltd | Shuo W.,Dalian Fusheng Natural Medicinal Development Co. Ltd | Li F.,Dalian Fusheng Natural Medicinal Development Co. Ltd
Chinese Journal of Cancer Biotherapy | Year: 2015

Objective:To study whether and how ginsenoside Rg3 regulate apoptosis of gastric cancer BGC-823 cells. Methods: BG C-823 cells were treated with vehicle (control),Rg3 (50 mg/ml),Ad-CaM,and Rg3 (50 mg/ml) plus Ad-CaM,respectively. At 48 hours after treatment,cell viability was assessed by MTT assay,cell apoptosis by flow cytometry ,cell invasive capacity by trans-well assay,CaM,Iκ B,CaMK II and NF B protein contents by Western blotting analysis. Results: Compared with vehicle control,Rg3 significantly promoted apoptosis and inhibited proliferation and invasion of BGC-823 cells (P <0. 05),while Ad-CaM and Rg3 + Ad-CaM significantly inhibited apoptosis and promoted proliferation and invasion of BGC-823 cells (P <0. 05). At the protein level,the expression of NF-k B and CaMK II was significantly downregulated whereas the expression of IκBα was significantly in Rg3-treated BGC-823 cells (P <0. 05), and the expression of NF-k B and CaMK II was significantly enhanced whereas the expression of IκBα was significantly inhibited in cells treated with Ad-CaM and Rg3 plus Ad-CaM respectively (P <0. 05), as compared with control cells. Conclusion: Rg3 may inhibit proliferation and invasion and promote apoptosis of gastric cancer cells through downregula-tion of CaM kinase expression and inactivation of NF B. © 2015, Editorial office of Chinese Journal of Cancer Biotherapy. All rights reserved. Source

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