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Wang L.,Friendship Hospital of Dalian | Liu C.-J.,Dalian Medical University | Luo Z.-J.,Changchun University
Journal of Dalian Medical University | Year: 2011

[Objective] To investigate the role of p38MAPK in the signal path way of low density ESW and low dose intermittent rhPTH1 - 34 stimulation's action on rat osteoblasts. [Methods] After stimulations of 0.18 mJ/mm2 ESW and 10-11 mol/L intermittent rhPTH1 - 34, the specific inhibitor of p38MAPK was added for finding the role of p38MAPK in the intracellular signal path way. The rat osteoblasts were collected, and cultured was detected, proliferation of these cells was observed by MTT and bone formation by measuring ALP activity. The expression of p - p38MAPK was detected by Western blot. [Results] Enhaning effect on bone formation by ESW can be significantly inhibited by SB203580, a inhibitor of p38MAPK (P<0.05). But the enhancement of ROBs' bone formation by intermittent rhPTH1 - 34 can not be inhibited by SB203580. Suitable ESW stimulation can improve the expression of p38MAPK, but intermittent rhPTH1 - 34 stimulation can not. [Conclusions] Suitable ESW stimulation can enhance bone formation of ROB by activation of p38MAPK. The enhancement of ROBs' bone formation by intermittent rhPTH1 - 34 stimulation dosen 't depend on activation of p38MAPK. Source


Wu S.,Friendship Hospital of Dalian | Zhang Y.,Dalian Medical University | Li B.,Friendship Hospital of Dalian | Fan L.-X.,Third Hospital of Dalian
Journal of Dalian Medical University | Year: 2015

Objective: To observe the inhibition effect of low dose CPT-11 combined with Sorafenib on the growth of human heptatic carcinoma strain HepG2 and the proliferation of human umbilical vascular endothelial cells (HUVECs). Methods: HUVECs and HepG2 cells were divided into 5 groups; control group, Sorafenib - treated group 6 μmol/L, CPT-11 (LDM) - treated group 30 μg/mL, CPT - 11 (TDM) - treated group 160 μg/mL and Sorafenib + CPT - 11 (LDM)-treated group. The cell proliferation inhibition rate was calculated with MTT assays. Results: Both Sorafenib and Low dose CPT-11 can inhibit the proliferation of HUVECs, and the inhibition rate in group sorafenib + CPT - 11 (LDM) - treated group higher than that in sorafenib group and CPT-11 (LDM) group (P <0.05). LDM chemotherapy had little inhibition effect on HepG2. The inhibition rate to HepG2 in CPT - 11 (LDM) group was significantly lower than that in others group (P < 0.05). Conclusion: The anti - tumour effects of Low - dose CPT - 11 could be from its angiogenesis inhibition. The combination of Sorafenib and CPT-11 (LDM) would be a better choice. Source


Lu X.-M.,Children Hospital of Dalian | Gao X.-J.,Friendship Hospital of Dalian | Jia L.,Friendship Hospital of Dalian | Song X.-H.,Friendship Hospital of Dalian | Lu M.,Friendship Hospital of Dalian
Chinese Journal of Medical Imaging Technology | Year: 2010

Objective: To investigate the value of strain and strain rate imaging in assessing contractile function and diastolic function of left ventricular and right ventricular in uremia patients. Methods: Thirty-four patients with uremia were divided into 2 groups according to whether left ventricular was hypertrophic: 18 patients in group of non-hypertrophic left ventricular and 16 patients in group of hypertrophic left ventricular. Thirty healthy adults were selected as control group. Strain (ε), systolic peak strain rate (SR s), early diastolic strain rate (SR e) and later diastolic strain rate (SR a) in the basal segments and middle segments of left and right ventriculars were measured. Results: In left ventricular, ε, SR s and SR e of each segments in group of non-hypertrophic left ventricular and hypertrophic left ventricular were smaller than those in control group, and in subtotal segments they were higher in group of non-hypertrophic left ventricular than those in group of hypertrophic left ventricular. In group of non-hypertrophic left ventricular, SR a was higher than that in group of hypertrophic left ventricular. In partial segments, SR a was smaller in group of non-hypertrophic left ventricular than that in control group, and in partial segments was higher than that in control group. In subtotal segments, SR a was smaller in group of hypertrophic left ventricular than that in control group. In right ventricular, ε, SR s and SR e in group of non-hypertrophic left ventricular and hypertrophic left ventricular in each segment were smaller than those in control group, and SR a was the lowest in normal group and the highest in group of hypertrophic left ventricular. Conclusion: Strain and strain rate imaging can assess contractile and diastolic functions of left and right ventriculars myocardium about uremia sensitively and quantitatively. Source

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