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Zhao Y.H.,ShenYang Agricultural University | Su K.,ShenYang Agricultural University | Guo Y.H.,ShenYang Agricultural University | Ma H.F.,Dalian Academy of Agricultural Science | Guo X.W.,ShenYang Agricultural University
Genetics and Molecular Research | Year: 2016

In this study, we aimed at finding the genetic regularity of grape maturation period. Early-maturing grapevine, “87-1”, was used as the female parent and late-maturing, “9-22”, as the male parent, to create an F1 hybrid population. A total of 149 individual plants and their parents were selected as the mapping population. Sequence-related amplified polymorphism and simple-sequence repeat analyses were performed. We performed a linkage analysis and constructed a molecular genetic map. In the obtained map, the female and male parents each covered 19 linkage groups containing 188 and 175 maker loci, respectively. The total map distances for the female and male parents were 1074.5 and 1100.2 cM, respectively, whereas the average genetic distances between each two loci were 5.7 and 7.8 cM, respectively. The interval-mapping method was used in a quantitative trait locus (QTL) analysis for fruit maturation period. A total of 12 QTLs associated with fruit maturation period were detected. These included four QTLs in the male parent genetic map that were located in linkage groups M5, M11, M14-1, and M16, with a 62.6-75.7% rate of contribution of each QTL. Another three QTLs were found in the female parent genetic map, located in linkage groups F6, F14-1, and F18, with a 72.7-77.7% rate of contribution of each QTL. Five more QTLs were detected in the consensus map, located in linkage groups LG11, LG14-1, LG16, LG17, and LG18, with 8.9-75.7% phenotypic variance explained by each QTL. © FUNPEC-RP. Source


Guo Y.,ShenYang Agricultural University | Xue R.,ShenYang Agricultural University | Lin H.,ShenYang Agricultural University | Su K.,ShenYang Agricultural University | And 7 more authors.
Pakistan Journal of Botany | Year: 2015

In this study, an F1 population was created by the cross ‘87-1’ב9-22’. The female parent ‘87-1’ was a black purple cultivar and the male parent was an excellent breeding line with green pericarp. the skin color separation of population and distribution, and determined the content of each individual fruit peel pigment. On the basis of the genetic map of Vitis vinifera L., We carried out the grape skin pigment content quantitative trait locus (QTL) analyses. The results show that the fruit color performance for continuous variation and the inheritance of fruit skin anthocyanidin content was a quantitative inheritance. The color of offspring ranges from green and black-blue and existing distribution. Using SSR and SRAP molecular markers to construct 188 female parent maps,175 male parent maps and 251 consensus maps, and the total map distance is 1047.5 cM,1100.2 cM and 1264.2 cM respectively. The result of QTL showed that there were more QTLs exist in the linkage group of 1, 2, 3, 4, 9, 13, 14, 16 and 19 and in the linkage group of 3, 4, 13 and 14, we detected QTLs in the similar position with the result of the study in the year of 2011 and 2012, and based on this we will conduct the fine QTL location in the future, this result will lay a good foundation for the grape in the department of molecular assistant breeding in the future. © 2015, Pakistan Botanical Society. All rights reserved. Source


Zhao Y.H.,ShenYang Agricultural University | Guo Y.S.,ShenYang Agricultural University | Lin H.,ShenYang Agricultural University | Liu Z.D.,ShenYang Agricultural University | And 6 more authors.
Genetics and Molecular Research | Year: 2015

A grapevine hybrid population was derived from a crossing of the early-maturing female parent cultivar ‘87-1’ and the late-maturing male parent cultivar ‘9-22’. A total of 149 plants were selected from the hybrid population as the mapping population, and after sequence-related amplified polymorphism and simple-sequence repeat marker analysis were conducted we constructed molecular genetic maps of the parents. The molecular linkage map of ‘87-1’ had 19 linkage groups that contained 188 markers, with an average interval of 5.7 cM and a total distance of 1074.5 cM; the ‘9-22’ map had 19 linkage groups that contained 175 markers, with an average interval of 7.8 cM and a total distance of 1100.2 cM. The molecular linkage map of both parents had 19 linkage groups that contained 251 markers, with an average interval of 5.0 cM and a total distance of 1264.2 cM. We used the interval mapping method to conduct a quantitative trait locus (QTL) analysis of grape weight and soluble solid content of the mapping population. Six QTLs were related to grape weight, and the average contribution to the phenotypic variance was between 11.3 and 33.0%. Seven QTLs were related to soluble solid content, and the average contribution to the phenotypic variance was between 15.7 and 55.8%. © FUNPEC-RP. Source


Xue K.,Dalian Medical University | Song J.,Dalian Medical University | Yang Y.,Dalian Medical University | Li Z.,Clinical Laboratory | And 3 more authors.
Molecular Immunology | Year: 2016

PAX5 is indispensable for the commitment of early lymphoid progenitors to the B cell lineage as well as for the development of B cells. Although previous studies have indicated that the Pax5-conditional-knockout mouse exhibited dedifferentiation of mature B cell and the development of aggressive lymphomas, the changes of Pax5 gene expressions in pre-B cells have not been analyzed. To understand the functional importance of Pax5 gene in the proliferation and survival of pre-B cells, we established a Pax5-knockdown model using 70Z/3 pre-B cell line. Pax5 knockdown 70Z/3 cells (70Z/3-KD cells) showed down-regulations of pre-BCR compounds such as CD19, BLNK, Id2 and λ5. The signaling via pre-BCRs was significantly diminished in the 70Z/3-KD cells, and this alteration was normalized by restored Pax5 gene expression. Loss of PAX5 reduced the growth rates in the 70Z/3-KD cells, compared to the mock cells. Meanwhile, the proliferation of pre-B cells was reduced by the knockdown of Pax5 gene. Moreover, further examinations showed that PAX5 was also activated in B cell acute lymphoblastic leukemia (B-ALL) as a cell proliferation enhancer. These findings suggested that pax5 is critically important for the proliferation and survival of pre-B cells. © 2016 Elsevier Ltd. Source


LI H.,Chinese Academy of Agricultural Sciences | QIU J.-J.,Chinese Academy of Agricultural Sciences | WANG L.-G.,Chinese Academy of Agricultural Sciences | XU M.-Y.,Dalian Academy of Agricultural Science | And 2 more authors.
Journal of Integrative Agriculture | Year: 2012

Agricultural production plays an important role in affecting atmospheric nitrous oxide (N2O) concentrations. Field measurements were conducted in Dalian City, Liaoning Province in Northeast China from two consecutive years (2009 and 2010) to estimate N2O emissions from a spring maize field, a main cropping system across the Chinese agricultural regions. The observed flux data in conjunction with the local climate, soil and management information were utilized to test a process-based model, DeNitrification-DeComposition (DNDC), for its applicability for the cropping system. The validated DNDC was then used for exploring strategies to reduce N2O emissions from the target field. The results showed that the major N2O pulse emissions occurred with duration of about 3-5 d after fertilizer application in both years 2009 and 2010, which on average accounted for about 60% of the total N2O emissions each year. Rainfall and fertilizer application were the major factors influencing the N2O emissions from spring maize field. The average N2O fluxes from the CK (control plot, without fertilization) and FP (traditional chemical N fertilizer) treatments were 23.1 and 60.6 μg m-2 h-1 in 2009, respectively, and 21.5 and 64.3 μg m-2 h-1 in 2010, respectively. The emission factors (EFs) of the applied N fertilizer (270 kg N ha-1) as N2O-N were 0.62% in 2009 and 0.77% in 2010, respectively. The comparison of modeled daily N2O emission fluxes against observations indicated that the DNDC model had a good performance even if without adjusting the internal parameters. The modeled results showed that management practices such as no-till, changing timing or rate of fertilizer application, increasing residue incorporation, and other technically applicable measures could effectively reduce N2O emissions from the tested fields. Our study indicated that avoiding application of N fertilizers at heavy rainfall events or splitting the fertilizer into more applications would be the most feasible approaches to reduce N2O emissions from spring maize production in Northeast China. © 2012 Chinese Academy of Agricultural Sciences. Source

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