Zhang D.-Q.,Dali University |
Zhou N.,Chongqing Three Gorges University
Biochemical Systematics and Ecology | Year: 2013
Taxus wallichiana var. mairei is an endangered conifer with important medicinal value in southern China. Nuclear SSR markers were employed to assess genetic diversity and structure of 13 geographically disjunct populations. The present study revealed a moderate genetic diversity (HE=0.538) and low genetic differentiation (FST=0.159). And most populations encountered in severe inbreeding and bottleneck effect. No significant genetic structure was detected by IBD and Structure analysis, which was supported by AMOVA analysis. The present results could be ascribed to an earlier period of more pronounced gene flow when the species had a more continuous distribution. However, the 13 studied populations were divided into four clusters based on the UPGMA dendrogram; these clusters were almost congruent with their geographical distributions. Vital areas such as southern mountains of Sichuan basin, Nanling Mts. and the margin of this yew's distribution range had a high priority for conservation. © 2013 Elsevier Ltd.
Yang Z.,University of Illinois at Urbana - Champaign |
Yang Z.,Dali University |
Sweedler J.V.,University of Illinois at Urbana - Champaign
Analytical and Bioanalytical Chemistry | Year: 2014
Early diagnosis is the key to the effective treatment of cancer. The detection of cancer biomarkers plays a critical role not only in cancer early diagnosis, but also in classification and staging tumor progression, or assessment prognosis and treatment response. Currently, various molecular diagnostic techniques have been developed for cancer biomarker studies, with many of the more effective approaches requiring a separation step before detection. Capillary electrophoresis (CE) can perform rapid and efficient separation with small samples, which is well-suited for analysis of both small- and macro- molecule biomarkers in complex samples. CE has different separation modes and can couple to different detectors into a variety of platforms, such as conducting studies on DNA/ RNA point mutation, protein misexpression, and metabolite abnormality. Similarly, microchip capillary electrophoresis (MCE) appears as a very important biomarker screening platform with the merits of high throughput, integration, and miniaturization, which makes it a promising clinical tool. By hyphenated different detectors, or integrated with immunoassay, PCR/LDR and related technologies, MCE can be constructed into diverse platforms used in genomics, proteomics, and metabolomics study for biomarkers discovery. The multiplex biomarker screening approach via CE- or MCE-based platforms is becoming a trend. This paper focuses on studies of cancer biomarkers via CE/MCE platforms, based on the studies published over the past 3 years. Some recent CE applications in the field of cancer study, such as cancer theranostics, are introduced. © 2014 Springer-Verlag Berlin Heidelberg.
Kaya S.,Dali University
Türkiye parazitolojii dergisi / Türkiye Parazitoloji Derneǧi = Acta parasitologica Turcica / Turkish Society for Parasitology | Year: 2011
Myiasis is a cause of urogenital parasitosis. In this study, a 29 year-old male patient who was admitted to our hospital with urinary discharge of worms has been presented. Larvae were examined parasitologically and urogenital myiasis caused by Psychoda albipennis larvae was diagnosed. No other pathological findings were detected in the patient and the symptoms resolved spontaneously within a few days. Despite the fact that urinary myiasis is very rare in humans, it should be considered in patients with urinary complaints. Apparently, a quick recovery without treatment is possible in most cases.
Gu W.,Dali University
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases | Year: 2012
To carry out clinical diagnosis and treatment of Fasciola gigantica infection during an outbreak in Yunnan Province. Data on epidemiology, diagnosis and treatment were collected from 27 patients. A questionnaire survey to the patients and partial villagers was carried out including history of raw food-eating and pet-raising. Animal feces were collected and examined by precipitation method and eggs incubation method. Cattle from two patients families were dissected to find Fasciola infection. Serum samples from patients, family members, and villagers were detected. Possible intermediate snails were collected from the vicinity of streams and ponds in 15 villages where patients lived. The earliest onset of symptoms among the patients was on March 10, 2011 and the last case was on January 10, 2012. The clinical manifestations were mainly fever with unknown reason, decreasing hemoglobin, increasing eosinophils, and hepatosplenomegaly. No parasite eggs were found in feces. Antibodies against F. gigantica were positive by ELISA in 23 patients. Fasciola eggs were then found in 4 patients' feces on February 16, 2012. F. gigantica eggs and adults were found in the hepatobiliary system of dissected cattle. Triclabendazole [10 mg/(kg x d) x2 d] was administered orally for the patients and the clinical symptoms eased. Snails including Physa acuta, Radix swinhoei and Galba pervia were collected and cercariae were found in only one snail. An outbreak of F. gigantica infection has been confirmed and the diagnosed cases effectively treated with triclabendazole.
Feng J.,Dali University |
Zhu Y.,Yunnan Agricultural University
Biodiversity and Conservation | Year: 2010
With a wide range of habitats and exploding development of international trade, China is facing escalating risk of the plant invasion. Research on the risk assessment of alien invasive plants may help us manage the invasive with priority, and is imperatively needed in China. However, until now, few studies have been conducted for the risk of alien invasive plants and its spatial patterns in China, especially with a regard to ecosystem conservation and biodiversity protection. In this paper, we assessed the risk of invasive plants and its spatial patterns. We focused on the following basic questions: Which alien invasive plants are the most noxious, and which of them are mild? How are the spatial patterns of alien invasive plants at different risk ranks? In the present study, the risk of 100 major alien invasive plants in China was assessed based on a revised risk assessment system and an extensive consult with literatures. The results indicated that there were 18 species of rank 1st, 29 of rank 2nd, 33 of rank 3rd and 20 of rank 4th. Although, there was high richness of alien invasive plants in South China, most of them were of low risk. By contrast, low richness of alien invasive plants was observed in North China and the regions in high altitude, but most of the invasive were of high risk. Compared with previous studies, our study appears to have delivered credible results and may play an early warning function, and also further our understanding of the risk of invasive plants and its spatial patterns in China, though we acknowledged that the risk assessment may have some potential weakness, and appealed timely and further investigations in future. © 2010 Springer Science+Business Media B.V.