Zeng W.T.,Sun Yat Sen University |
Zheng Q.S.,Sun Yat Sen University |
Huang M.,Sun Yat Sen University |
Cen H.J.,Guangzhou Women and Childrens Medical Center |
And 4 more authors.
Pharmazie | Year: 2012
Background: VKORC1, CYP2C9 and CYP4F2 are three critical genes associated with inter-individual variation of warfarin dose. Many dosing algorithms containing these gene polymorphisms and demographic characteristics have been set up for better use of warfarin. However, with distinct genemutation frequencies among different ethnics, dosing algorithms differ greatly. For Chinese, related research just concentrate on Han Chinese, ignoring other Chinese ethnicities. This study aims to detect the popular polymorphisms in these three critical genes in Bai, Tibetan Chinese, to start the exploration of better use of warfarin in Chinese minorities. Methods: PCR-based methods were used to analyze VKORC1 3673G > A, CYP2C9*3, CYP4F2 rs2108622 C > T in Han, Bai and Tibetan Chinese. Results: The differences among the mutation frequencies of the studied genes in three ethnicities were not statistically significant. The frequency of A-allele of VKORC1 3673G > A was 92.8%, 90.2%, 90.8% in Bai, Tibetan, Han Chinese, respectively. The frequency of *3-allele in CYP2C9*3 was low in Bai (4.5%), Tibetan (2.8%) and Han Chinese (4.6%). Approximately one fourth of each ethnic had themutant T-allele of CYP4F2 rs108622. However, Bai Chinese got statistically higher A-allele frequency of VKORC1 3673G > A than previously studied Han Chinese did. Conclusions: Bai Chinese got significant higher A-allele frequency of VKORC1 3673G > A.
Yang M.,Yunnan University |
Yang M.,Dali College |
Cai L.,Yunnan University |
Tai Z.,Yunnan University |
And 2 more authors.
Fitoterapia | Year: 2010
Three biphenanthrene compounds (1-3) and a triphenanthrene compound (4), together with six known biphenanthrene compounds (5-10), were isolated from the tubers of Monomeria barbata Lindl. Their structures were elucidated on the basis of extensive spectroscopic analysis (1D-, 2D-NMR, and HR-ESI-MS). These four new compounds were tested in vitro for the cytotoxic activity against liver carcinoma (HepG-2), promyelocytic leukaemia (HL60), ovarian carcinoma (Skov-3) and epidermoid carcinoma (A431) cancer cell lines and the antioxidant activity in DPPH radical scavenging. Compounds 1-4 exhibited significant cytotoxic activity against HepG-2 and HL60 cell lines, and potent antioxidant activity in DPPH radical scavenging. © 2010 Elsevier B.V.
Zhao C.,Kunming General Hospital of former Chengdu Military Region |
Zhang G.,Dali College |
Ma W.,Kunming General Hospital of former Chengdu Military Region |
Liu W.,Kunming General Hospital of former Chengdu Military Region |
Hong Y.,Kunming General Hospital of former Chengdu Military Region
Pharmaceutical Care and Research | Year: 2016
Objective: To analyze the drug use in the Department of Anesthesiology in Kunming General Hospital of former Chengdu Military Region, so as to provide reference for rational drug application and standardized management. Methods: Analyses were made on the consumed drug categories, per capita consumption sum of drugs, frequency of drug use and cost distribution in the Department of Anesthesiology from 2009 to 2014, by using Excel 2007 software, defined daily dose (DDD) and ABC analysis. Results: The drug use in Department of Anesthesiology tended to be more rational and standardized. The annual per capita cost of drugs and the number of drug categories tended to be lower. With respect to drug categories, frequency of drug use and cost distribution, the drug application in the Department of Anesthesiology was basically rational. Due to such factors as the transformation of anesthesia modes, there were considerable changes in the application of certain surgical drugs. The consumption sum of a few non-essential anesthetic drugs decreased dramatically or even dropped to zero. Conclusion:: The drug control measures adopted by the Department of Anesthesiology achieved rather good results, which would provide reference for rational use of anesthetics.
Zeng J.,Dali College |
Peng F.,Dali College
Pharmaceutical Care and Research | Year: 2013
Hepatitis C(HC) is a communicable disease with high morbidity and high mortality. Approximately 180 million people are infected with hepatitis C virus (HCV) across the globe, however, there is still no effective treatment. This review gives an outline of the research advances in anti-HCV drugs, including interferon combined with ribavirin, specific targeted therapy drugs, immune modulators and so on.
He Z.,Zhejiang University |
He Z.,Dali College |
Liang F.,Zhejiang University |
Lu J.,Zhejiang University |
Pan Y.,Zhejiang University
European Journal of Medicinal Chemistry | Year: 2013
Five new oleanane-type triterpenoids, including two aglycones, 13β-28-epoxy-3β,22α,23-trihyd roxyolean-16-one (1) and 13β-28-epoxy-22α,23-dihydroxyolean-3,16-dione (2), and three glycosides, anagalligenone-3-O-α-L-arabinopyranoside (3), anagalligenone-3-O-[β-D-glucopyranosyl(1 → 4)-α-L- arabinopyranoside] (4) and anagalligenone-3-O-[β-D-xylopyranosyl(1 → 2)-β-D-glucopyranosyl(1 → 4)-α-L-arabinopyranoside] (5), were isolated from the aerial parts of Lysimachia parvifolia, together with three known oleanane-type triterpenoid glycosides (6-8). The structures of the new compounds were subsequently elucidated by spectroscopic analysis and their cytotoxicities evaluated against six human cancer cell lines. Compounds 5-8 exhibited significant cytotoxicities against all the cell lines tested, with IC50 values lower than 10 μM. The possible mechanism of action of compound 6 was also studied. © 2013 Elsevier Masson SAS. All rights reserved.
Yang M.H.,Nature Technology |
Yang M.H.,Dali College |
Cai L.,Nature Technology |
Li M.H.,Nature Technology |
And 3 more authors.
Chinese Chemical Letters | Year: 2010
Three new monophenanthrene compounds were isolated from an acetone extract of the tubers of Monomeria barbata Lindl. (Orchidaceae). Using spectroscopic methods, the structures of compounds 1-3 were determined as 1,4,7-trihydroxy-2-methoxy-9,10-dihydrophenanthrene (1), 1-(p-hydroxybenzoyl)-2-methoxy-4,7-dihydroxy-9,10-dihydrophenanthrene (2), 1,3,8-tri(p-hydroxybenzyl)-4-methoxy-phenanthrene-4,7-diol (3). These compounds showed cytotoxicities on four tumor cell lines (HepG2, HL-60, Skov-3, A431). © 2009 Zhong Tao Ding.
He J.-B.,CAS Kunming Institute of Botany |
He J.-B.,Dali College |
Liu G.-M.,Dali College |
Cheng Y.-X.,CAS Kunming Institute of Botany
Chinese Traditional and Herbal Drugs | Year: 2011
Objective: To study the chemical constituents of Vespae Nidus. Methods: The compounds were isolated by various chromatographic techniques and the structures were elucidated on the basis of spectral analysis. Results: Sixteen compounds were isolated from 95% ethanol extract of Vespae Nidus by silica gel, Sephadex LH-20, and RP-18 column chromatography. They were identified as anisaldehyde (1), 2, 4-dihydroxy-3, 6-dimethylbenzoate (2), 1, 4-dihydroxy-2-methoxy benzene (3), 2-(4-methoxyphenyl) acetic acid (4), glycerol (5), tyrosol (6), 3, 5-dihydroxy-1, 7-bis (4-hydroxyphenyl) heptane (7), vomifoliol (8), clemaphenol A (9), N-benzoyl-L-phenylalaninol (10), asperglaucide (11), (2R, 3S)-2-(3', 4'-dihydroxyphenyl)-3-acetylamino-7-hydroxyethyl-1,4-benzodioxane (12), ursolic acid (13), β-sitosterol (14), thymidine (15), and neoechinulin A (16). Conclusion: Compounds 1-13 and 16 are isolated from Vespae Nidus for the first time.
Huang J.-X.,Dali College |
Scott M.B.,Himalaya |
Pu X.-Y.,Dali College |
Zhou-Cun A.,Dali College
Reproductive BioMedicine Online | Year: 2012
The gene for DNA methyltransferase 3-like protein (DNMT3L) is essential for normal spermatogenesis and may be involved with spermatogenetic impairment and male infertility. To explore the possible association between the DNMT3L gene and male infertility, this study investigated allele, genotype and haplotype frequencies of three single nucleotide polymorphism (SNP) loci, rs2070565, rs2276248 and rs7354779, of DNMT3L in 233 infertile patients with azoospermia and 249 fertile controls from a population of Chinese men using polymerase chain reaction/restriction fragment length polymorphism. Results showed that the frequencies of allele A (20.6% versus 14.9%; P = 0.022) and the allele A carrier (GA + AA; 37.8% versus 28.1%; P = 0.027) in azoospermic patients were significantly higher than those in controls at the rs2070565 locus. The haplotype AAA frequency was significantly higher (18.1% versus 12.4%; P = 0.02) while the haplotype GAA frequency was significantly lower (53.2% versus 62.1%; P = 0.007) in infertile patients compared with fertile controls. These results indicated that SNP rs2070565, as well as haplotypes AAA and GAA, may be associated with male infertility and suggest that DNMT3L may contribute to azoospermia susceptibility in humans. The gene for DNA methyltransferase 3-like protein (DNMT3L) plays an important role in DNA methylation of some genes during spermatogenesis, essential for spermatozoa maturation in male. Defects of DNMT3L have been shown to lead to spermatogenic impairment and subsequent male infertility in mice. So, it is reasonable to postulate that DNMT3L may also involved with impaired spermatogenesis and infertility in humans. To test this, we investigated the allele, genotype and haplotype frequencies of three single nucleotide polymorphism (SNP) loci, rs2070565, rs2276248 and rs7354779, of DNMT3L in a population of Chinese men that included 249 fertile control individuals and 233 individuals with azoospermia. At the rs2070565 locus, the frequencies of allele A and the allele A carrier (GA + AA) in patients were significantly higher than those in controls, implying that the presence of rs2070565 allele A may increase the risk of azoospermia in males. Also, significant differences were detected in the distribution of haplotypes of the three SNP between patients and controls. Haplotype AAA was significantly higher while haplotype GAA was significantly lower in infertile individuals in comparison to control individuals, indicating that haplotype AAA might be a risk factor for azoospermia, while haplotype GAA may provide have some protection against azoospermia. These results suggest that DNMT3L may be contribute to susceptibility of azoospermia in male humans. © 2011, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
Li Y.H.,Dali College |
Liu Y.J.,Yunnan University |
Kan H.E.,Yunnan University |
Liu Q.J.,Yunnan University |
Zhao H.Y.,Yunnan University
Materials Technology | Year: 2012
A synthesising method is reported with an enhanced solid state reaction to fabricate SnO 2 nanorods. The structure and morphology of the SnO 2 nanorods were characterised by X-ray diffraction and transmission electron microscopy. The results showed that the nanorods were rutile SnO 2 with a diameter of ,30 nm and length of several micrometres. It was found that chlorine salt was very important for controlling the grain size of nanoparticles and providing kinetic and ambulatory circumstance to induce oriented growth of SnO 2 nanoparticles. The growth mechanism of the nanorods can be understood on the basis of oriented aggregation induced by polar forces. © 2012 W. S. Maney & Son Ltd.
Li Y.,Yunnan University |
Li Y.,Dali College |
Liu Y.,Yunnan University |
Xiao X.,Yunnan University |
And 2 more authors.
Advanced Materials Research | Year: 2012
SnO 2 nanorods with rutile structure were successfully synthesized by solid state reaction in the presence of NaCl-KCl. Indirect-heating sensors based on nanorods were fabricated and investigated for the gas sensing properties to ethanol. The results showed that the response of sensor to 1000 ppm ethanol is up to 114.3, the export voltage to 1 ppm ethanol can attain 2.0 V at 275 °C, and the response time and recovery time are no longer than 10 s.