Dali College

Dali, China

Dali College

Dali, China

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He Z.,Zhejiang University | He Z.,Dali College | Liang F.,Zhejiang University | Lu J.,Zhejiang University | Pan Y.,Zhejiang University
European Journal of Medicinal Chemistry | Year: 2013

Five new oleanane-type triterpenoids, including two aglycones, 13β-28-epoxy-3β,22α,23-trihyd roxyolean-16-one (1) and 13β-28-epoxy-22α,23-dihydroxyolean-3,16-dione (2), and three glycosides, anagalligenone-3-O-α-L-arabinopyranoside (3), anagalligenone-3-O-[β-D-glucopyranosyl(1 → 4)-α-L- arabinopyranoside] (4) and anagalligenone-3-O-[β-D-xylopyranosyl(1 → 2)-β-D-glucopyranosyl(1 → 4)-α-L-arabinopyranoside] (5), were isolated from the aerial parts of Lysimachia parvifolia, together with three known oleanane-type triterpenoid glycosides (6-8). The structures of the new compounds were subsequently elucidated by spectroscopic analysis and their cytotoxicities evaluated against six human cancer cell lines. Compounds 5-8 exhibited significant cytotoxicities against all the cell lines tested, with IC50 values lower than 10 μM. The possible mechanism of action of compound 6 was also studied. © 2013 Elsevier Masson SAS. All rights reserved.


Yang M.H.,Nature Technology | Yang M.H.,Dali College | Cai L.,Nature Technology | Li M.H.,Nature Technology | And 3 more authors.
Chinese Chemical Letters | Year: 2010

Three new monophenanthrene compounds were isolated from an acetone extract of the tubers of Monomeria barbata Lindl. (Orchidaceae). Using spectroscopic methods, the structures of compounds 1-3 were determined as 1,4,7-trihydroxy-2-methoxy-9,10-dihydrophenanthrene (1), 1-(p-hydroxybenzoyl)-2-methoxy-4,7-dihydroxy-9,10-dihydrophenanthrene (2), 1,3,8-tri(p-hydroxybenzyl)-4-methoxy-phenanthrene-4,7-diol (3). These compounds showed cytotoxicities on four tumor cell lines (HepG2, HL-60, Skov-3, A431). © 2009 Zhong Tao Ding.


Huang J.-X.,Dali College | A Z.-C.,Dali College
Chinese Journal of Medical Genetics | Year: 2010

Objective: To study the relationship between the polymorphisms of single nucleotide polymorphisms (SNPs) in rs2240424, rs2057951, rs2240427 and rs714909 loci in the PATZ1 gene and azoospermia. Methods: The allele and genotype frequencies of the four SNPs were investigated in 180 patients with azoospermia and 190 normal men as controls by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis. Results: The frequencies of allele C (35.0% vs. 27.6%, P = 0.031) and individuals with allele C (TC+CC) (57.8% vs. 46.3%, P = 0.027) at the rs2057951 locus in azoospermic patients were significantly higher than that in normal men. There was a significant difference in distribution of haplotypes of the four SNPs between the two groups (P = 0.01). Hapoltypes ACAC (11.1% vs. 6.6%, P = 0.029) and ACGC (11.2% vs. 5.2%, P = 0.003) increased significantly in azoospermic patients compared with controls. Conclusion: The allele C of rs2057951 locus and haplotypes ACAC and ACGC of the four SNPs in PTAZ1 gene increased the susceptibility to azoospermia, suggesting that PATZ1 gene may be associated with azoospermia.


He J.-B.,CAS Kunming Institute of Botany | He J.-B.,Dali College | Liu G.-M.,Dali College | Cheng Y.-X.,CAS Kunming Institute of Botany
Chinese Traditional and Herbal Drugs | Year: 2011

Objective: To study the chemical constituents of Vespae Nidus. Methods: The compounds were isolated by various chromatographic techniques and the structures were elucidated on the basis of spectral analysis. Results: Sixteen compounds were isolated from 95% ethanol extract of Vespae Nidus by silica gel, Sephadex LH-20, and RP-18 column chromatography. They were identified as anisaldehyde (1), 2, 4-dihydroxy-3, 6-dimethylbenzoate (2), 1, 4-dihydroxy-2-methoxy benzene (3), 2-(4-methoxyphenyl) acetic acid (4), glycerol (5), tyrosol (6), 3, 5-dihydroxy-1, 7-bis (4-hydroxyphenyl) heptane (7), vomifoliol (8), clemaphenol A (9), N-benzoyl-L-phenylalaninol (10), asperglaucide (11), (2R, 3S)-2-(3', 4'-dihydroxyphenyl)-3-acetylamino-7-hydroxyethyl-1,4-benzodioxane (12), ursolic acid (13), β-sitosterol (14), thymidine (15), and neoechinulin A (16). Conclusion: Compounds 1-13 and 16 are isolated from Vespae Nidus for the first time.


Kan H.E.,Yunnan University | Zhao Y.F.,Yunnan University | Liu Y.J.,Yunnan University | Li Y.H.,Yunnan University | And 2 more authors.
Materials Technology | Year: 2012

Orthogonal perovskite EuFe 0.9Co 0.1O 3 oxides with uniform particle size distribution were prepared by sol-gel method. The samples were calcined at different temperatures, and the results showed that the mean grain size increased from 31 to 38 nm when the calcining temperature was increased from 600 to 900°C. Investigation of the sensing properties exhibited that the sensor based on EuFe 0.9Co 0.1O 3 calcined at 800°C has good acetone gas sensing properties at an operating temperature of 280°C. © 2012 W. S. Maney & Son Ltd.


Huang J.-X.,Dali College | Scott M.B.,Himalaya | Pu X.-Y.,Dali College | Zhou-Cun A.,Dali College
Reproductive BioMedicine Online | Year: 2012

The gene for DNA methyltransferase 3-like protein (DNMT3L) is essential for normal spermatogenesis and may be involved with spermatogenetic impairment and male infertility. To explore the possible association between the DNMT3L gene and male infertility, this study investigated allele, genotype and haplotype frequencies of three single nucleotide polymorphism (SNP) loci, rs2070565, rs2276248 and rs7354779, of DNMT3L in 233 infertile patients with azoospermia and 249 fertile controls from a population of Chinese men using polymerase chain reaction/restriction fragment length polymorphism. Results showed that the frequencies of allele A (20.6% versus 14.9%; P = 0.022) and the allele A carrier (GA + AA; 37.8% versus 28.1%; P = 0.027) in azoospermic patients were significantly higher than those in controls at the rs2070565 locus. The haplotype AAA frequency was significantly higher (18.1% versus 12.4%; P = 0.02) while the haplotype GAA frequency was significantly lower (53.2% versus 62.1%; P = 0.007) in infertile patients compared with fertile controls. These results indicated that SNP rs2070565, as well as haplotypes AAA and GAA, may be associated with male infertility and suggest that DNMT3L may contribute to azoospermia susceptibility in humans. The gene for DNA methyltransferase 3-like protein (DNMT3L) plays an important role in DNA methylation of some genes during spermatogenesis, essential for spermatozoa maturation in male. Defects of DNMT3L have been shown to lead to spermatogenic impairment and subsequent male infertility in mice. So, it is reasonable to postulate that DNMT3L may also involved with impaired spermatogenesis and infertility in humans. To test this, we investigated the allele, genotype and haplotype frequencies of three single nucleotide polymorphism (SNP) loci, rs2070565, rs2276248 and rs7354779, of DNMT3L in a population of Chinese men that included 249 fertile control individuals and 233 individuals with azoospermia. At the rs2070565 locus, the frequencies of allele A and the allele A carrier (GA + AA) in patients were significantly higher than those in controls, implying that the presence of rs2070565 allele A may increase the risk of azoospermia in males. Also, significant differences were detected in the distribution of haplotypes of the three SNP between patients and controls. Haplotype AAA was significantly higher while haplotype GAA was significantly lower in infertile individuals in comparison to control individuals, indicating that haplotype AAA might be a risk factor for azoospermia, while haplotype GAA may provide have some protection against azoospermia. These results suggest that DNMT3L may be contribute to susceptibility of azoospermia in male humans. © 2011, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.


Li Y.H.,Dali College | Liu Y.J.,Yunnan University | Kan H.E.,Yunnan University | Liu Q.J.,Yunnan University | Zhao H.Y.,Yunnan University
Materials Technology | Year: 2012

A synthesising method is reported with an enhanced solid state reaction to fabricate SnO 2 nanorods. The structure and morphology of the SnO 2 nanorods were characterised by X-ray diffraction and transmission electron microscopy. The results showed that the nanorods were rutile SnO 2 with a diameter of ,30 nm and length of several micrometres. It was found that chlorine salt was very important for controlling the grain size of nanoparticles and providing kinetic and ambulatory circumstance to induce oriented growth of SnO 2 nanoparticles. The growth mechanism of the nanorods can be understood on the basis of oriented aggregation induced by polar forces. © 2012 W. S. Maney & Son Ltd.


Wang G.-F.,Dali College | Xue S.-P.,Dali College | Wu L.-X.,Dali College
Chinese Journal of Biologicals | Year: 2012

Objective: To investigate the distribution of drug-resistance and mechanism of fluoroqninolones-resistance of Shigella isolates in Dali Region, Yunnan Province, China. Methods: Fecal specimens were collected from various hospitals in Dali Region in 2010-2011, from which Shigella strains were isolated, cultured and identified for serotype. The resistances of Shigella isolates to 12 routine antibacterial drugs were analyzed by K-B agar diffusion test. The minimal inhibitory concentrations (MICs) of ceflriaxome and nalidixic acid to drug-resistant Shigella strains were determined by E-test. The gene mutations of drug-resistant strains were analyzed by PCR and DNA sequencing. The relationship between resistance to fluoroqninolones and R plasmid mediation was evaluated by R plasmid conjugation test. Results: A total of 68 Shigella strains were isolated, including 41 Shigella flexner strains, 25 Shigella sonnei strains and 2 Shigella boydii strains. The resistance rate of Shigella to nalidixic acid was the highest, followed by those to sulfalene and nalidixic. The Shigella isolates were relatively sensitive to ceftriaxone and cefotaxime, while showed a certain resistance to fluoroquinolones. The MICs of ciprofloxacin to Shigella strains ranged from 0.008 to 32 μg/ml. However, all the MICs of nalidixic acid to Shigella strains were more than 1 μg/ml, of which those to 4 strains more than 256 μg/ml. Substitutions of Ser83 → Leu in gyrA gene and Ser80 → Ile in parC gene were observed in 6, while those of Asp87 → Asn in gyrA gene in 2, Asp87 → Gly of gyrA gene in 2, and Gln91 → His of parC gene in 3 fluoroquinolones-resistant strains. No evidence suggested that R plasmid mediated the resistance to fluoroquinolones. Conclusion: Ceftriaxone and cefotaxime were the first choice for treatment of bacterial dysentery. The fluoroquinolone-resistance of Shigella was closely associated with the mutations of gyrA and parC genes, while was unassociated with R plasmid mediation.


Li Y.,Yunnan University | Li Y.,Dali College | Liu Y.,Yunnan University | Xiao X.,Yunnan University | And 2 more authors.
Advanced Materials Research | Year: 2012

SnO 2 nanorods with rutile structure were successfully synthesized by solid state reaction in the presence of NaCl-KCl. Indirect-heating sensors based on nanorods were fabricated and investigated for the gas sensing properties to ethanol. The results showed that the response of sensor to 1000 ppm ethanol is up to 114.3, the export voltage to 1 ppm ethanol can attain 2.0 V at 275 °C, and the response time and recovery time are no longer than 10 s.


He Z.,Zhejiang University | He Z.,Dali College | Liang F.,Zhejiang University | Zhang Y.,Zhejiang University | Pan Y.,Zhejiang University
Carbohydrate Research | Year: 2014

Four water-soluble polysaccharides, FCp-1, FCp-2, FCp-3, and FCp-4 were obtained from finger citron fruits (Citrus medica L. var. sarcodactylis) by hot-water extraction and ethanol precipitation, followed by routine separation procedure. Based on the calibration curve, molecular weights of them were estimated to be 113.9, 32.6, 140.3, and 177.1 kDa respectively. The acid hydrolysis, methylation, IR, GC-MS, and NMR experiments were used for composition analysis. FCp-1 was a heteropolysaccharide composed of arabinose, galactose, glucose, rhamnose, and xylose, with a molar ratio of 3.0:7.0:4.1:1.0:1.5. FCp-2 and FCp-4 were →4)-α-d-GalpA(1→ linking galacturonan differ in molecular weights. FCp-3 was a →6)-α-d-Glcp(1→ linking glucan. According to the results of in vitro assays, FCp-3 showed significantly and moderately enhancing capacities toward the proliferation of splenocytes and thymocytes respectively. Thus, FCp-3 or analogs may have further use as immunomodulatory agents. © 2014 Published by Elsevier Ltd. All rights reserved.

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