Dali Branch of Yunnan Provincial Tobacco Corporation

Dali, China

Dali Branch of Yunnan Provincial Tobacco Corporation

Dali, China

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Li Y.,Henan Agricultural University | Chang D.,Henan Agricultural University | Chang D.,Pingdingshan Branch of Henan Provincial Tobacco Company | Sun J.,Dali Branch of Yunnan Provincial Tobacco Company | And 3 more authors.
Tobacco Science and Technology | Year: 2017

To investigate the nitrogen utilization characteristics of different tobacco types and varieties and establish the reasons behind different nitrogen utilization efficiency, experiments using two tobacco cultivars, Hongda (flue-cured tobacco) and TN86 (burley tobacco) of different nitrogen efficiencies were carried out in a floating system. The activities of key enzymes involving nitrogen metabolism, pigment content, nitrogen accumulation and biomass of seedlings of the two cultivars were determined. The results showed that: 1) The seedlings of flue-cured tobacco cv. Hongda had a higher biomass, glutamine synthetase (GS) activity and pigment content, a stronger nitrogen assimilating capacity, a larger nitrogen accumulation amount and great nitrogen utilization efficiency. 2) The seedlings of burley tobacco cv. TN86 had a lower biomass, glutamine synthetase activity and pigment content, a higher nitrate reductase (NR) activity, a stronger nitrate reduction capacity, a weaker nitrogen assimilating capacity, a higher total nitrogen content, a low nitrogen accumulation amount and nitrogen utilization efficiency. The correlation analysis suggested that glutamine synthetase activity, nitrate reductase activity and pigment content closely correlated to nitrogen accumulation and utilization efficiency, especially the correlation of glutamine synthetase activity and chlorophyll content with nitrogen accumulation and utilization efficiency. Therefore, promoting pigment contents and the nitrogen assimilation activity could be used as an approach for cultivation technology and genetic variety improvement. © 2017, Editorial Office of Tobacco Science and Technology. All right reserved.


Wei S.,Henan Agricultural University | Wang D.,Dali Branch of Yunnan Provincial Tobacco Company | Su J.,Dali Branch of Yunnan Provincial Tobacco Company | Li Z.,Dali Branch of Yunnan Provincial Tobacco Company | And 5 more authors.
Tobacco Science and Technology | Year: 2016

In order to study the moisture variations in midrib of tobacco leaves during flue-curing, low-field nuclear magnetic resonance(LF-NMR) was used to determine the content and state of moisture, and the results were compared with those determined by a conventional oven method. The results showed that: 1) The moisture in midrib of fresh green tobacco leaves included bound water T21(1-10 ms) and free water T22(100-1 000 ms). During flue-curing, the signal amplitude of free water M22 and total water M20 decreased, and the signal amplitude of bound water M21 increased first then decreased. 2) Linear regression analysis indicated that the total signal amplitude of inversion spectrum positively correlated to the moisture content and dry weight base moisture content at a significant degree (coefficient of determination R2=0.997 9, R2=0.998 3, P<0.01). The moisture content in leaves during flue-curing could be obtained by the regression equation obtained. 3) Comparing with the oven method, the stability of LF-NMR was better. LF-NMR is thus suitable for the rapid and accurate determination of moisture contents in midrib of tobacco leaves during flue-curing. © 2016, Editorial Office of Tobacco Science and Technology. All right reserved.


Xu D.,Henan Agricultural University | Sun J.,Dali Branch of Yunnan Provincial Tobacco Company | Yang H.,Henan Agricultural University | Xu F.,Dali Branch of Yunnan Provincial Tobacco Company | And 3 more authors.
Tobacco Science and Technology | Year: 2016

In order to effectively control the nitrogen metabolism in overgrown tobacco plants at mature stage and promote the quality of flue-cured leaves, field trials were conducted between 2013-2014 to investigate the effects of an enzyme inhibitor, 4-[hydroxyl(methyl)phosphinoyl]-DL-homoalanine (glufosinate). The activities of nitrate reductase (NR) and glutamine synthetase (GS), key enzymes for nitrogen metabolism, and the chemical components in tobacco leaves were determined. In addition, sensory quality of treated flue-cured tobacco leaves for cigarettes was panel tested. The results showed that spraying the enzyme inhibitor at different concentrations at mature stage effectively decreased NR and GS activities in tobacco leaves, and the concentration of the enzyme inhibitor significantly negatively correlated to the NR and GS activities. The NR and GS activities declined quickly in the treated leaves and good field performance was achieved at the enzyme inhibitor concentrations between 10 and 15 mg/L. After spraying at the appropriate concentrations at mature stage, the contents of protein, nicotine and total nitrogen decreased, while those of reducing sugar and total sugar increased in the flue-cured leaves. The leaf chemical composition was more harmonious, the pigment content was reduced and the contents of neutral aroma components increased by 25.04%-42.76%. These have resulted in an increased score of sensory by 7.22%-10.43%. Considering cost and safety, 10 mg/L was recommended as a more appropriate concentration. © 2016, Editorial Office of Tobacco Science and Technology. All right reserved.


Wang X.,Dali Branch of Yunnan Provincial Tobacco Corporation | Fan Z.,Dali Branch of Yunnan Provincial Tobacco Corporation | Li Z.,Dali Branch of Yunnan Provincial Tobacco Corporation | Hu Y.,Dali Branch of Yunnan Provincial Tobacco Corporation | And 3 more authors.
Tobacco Science and Technology | Year: 2015

In order to better know the distribution of available zinc in Dali tobacco growing soil and control zinc balance in soil reasonably, 1 131 soil samples were collected and analyzed. The results showed that: 1) The average content of available zinc was of 3.11 mg/kg ranged from 0.07 to 72.92 mg/kg with a variation coefficient of 146.95%. Only Yunlong, Yongping, Yangbi, Xiangyun, Weishan and Binchuan counties were zinc-deficient. 2) Soil types in the order of available zinc content were purple soil > paddy soil > red soil > alluvial soil > yellow soil. 3) By altitudes, the order of available zinc content were high altitude (>2 100 m) > low altitude (<1 700 m) > medium altitude (1 700-2 100 m). 4) Soil with pH of 5.0-7.0 was of higher available zinc content, which accounted for 70.55% of all samples. 5) The content of available zinc increased with the increase of organic matter content in soil. ©, 2015, Editorial Office of Tobacco Science and Technology. All right reserved.

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