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Prague, Czech Republic

Srutkova D.,Academy of Sciences of the Czech Republic | Schwarzer M.,Academy of Sciences of the Czech Republic | Hudcovic T.,Academy of Sciences of the Czech Republic | Zakostelska Z.,Academy of Sciences of the Czech Republic | And 5 more authors.
PLoS ONE | Year: 2015

Background Reduced microbial diversity has been associated with inflammatory bowel disease (IBD) and probiotic bacteria have been proposed for its prevention and/or treatment. Nevertheless, comparative studies of strains of the same subspecies for specific health benefits are scarce. Here we compared two Bifidobacterium longum ssp. longum strains for their capacity to prevent experimental colitis. Methods Immunomodulatory properties of nine probiotic bifidobacteria were assessed by stimulation of murine splenocytes. The immune responses to B. longum ssp. longum CCM 7952 (Bl 7952) and CCDM 372 (Bl 372) were further characterized by stimulation of bone marrowderived dendritic cell, HEK293/TLR2 or HEK293/NOD2 cells. A mouse model of dextran sulphate sodium (DSS)-induced colitis was used to compare their beneficial effects in vivo. Results The nine bifidobacteria exhibited strain-specific abilities to induce cytokine production. Bl 372 induced higher levels of both pro- And anti-inflammatory cytokines in spleen and dendritic cell cultures compared to Bl 7952. Both strains engaged TLR2 and contain ligands for NOD2. In a mouse model of DSS-induced colitis, Bl 7952, but not Bl 372, reduced clinical symptoms and preserved expression of tight junction proteins. Importantly, Bl 7952 improved intestinal barrier function as demonstrated by reduced FITC-dextran levels in serum. Conclusions We have shown that Bl 7952, but not Bl 372, protected mice from the development of experimental colitis. Our data suggest that although some immunomodulatory properties might be widespread among the genus Bifidobacterium, others may be rare and characteristic only for a specific strain. Therefore, careful selection might be crucial in providing beneficial outcome in clinical trials with probiotics in IBD. © 2015 Srutkova et al. Source


Michlova T.,Czech University of Life Sciences | Dragounova H.,Dairy Research Institute Ltd. | Hejtmankova A.,Czech University of Life Sciences
Agronomy Research | Year: 2015

In this article, the influence of different ways of storage on the content of vitamin A and E in powdered cow´s milk was studied. The cow´s whole milk powder was taken directly from the manufacturer and stored for one year in 4 different ways – in the light at room temperature, in the dark at room temperature, in a refrigerator at 8°C and in a freezer at -20°C. The content of vitamins was measured 4 times during the first month and then once a month. The samples were stored for one year. Vitamins A and E were determined by HPLC using DAD and FLD detectors. Vitamin A was identified in all samples but only α-tocopherol (out of various forms of vitamin E) was detected in all samples. In all cases steeper decline of both vitamins in first 14 days of storage was identified. The highest losses of vitamin A and E in powdered milk occurred during storage in the light at room temperature. The value decreased by 91 resp. 95% of the original value. © 2015, Eesti Pollumajandusulikool. All rights reserved. Source


Michlova T.,Czech University of Life Sciences | Dragounova H.,Dairy Research Institute Ltd. | Hornickova S.,Czech University of Life Sciences | Hejtmankova A.,Czech University of Life Sciences
Czech Journal of Food Sciences | Year: 2015

The content of lipophilic vitamins A and E was determined in samples of sheep and goat milk of different breeds coming from 9 farms in central, eastern, and southern Bohemia. Samples were collected throughout the period of lactation (from April to September). Vitamins A and E were determined by HPLC using DAD and FLD detectors. Vitamin A was determined in all samples but only α-tocopherol (out of various forms of vitamin E) was detected in all samples. The total average content of vitamins A and E in raw milk of all sheep breeds during lactation was 0.93 ± 0.07 and 2.93 ± 0.87 mg/kg, levels of these vitamins in goat milk were 0.79 ± 0.08 and 1.29 ± 0.35 mg/kg, respectively. The results showed a significantly medium and strong correlation between the content of vitamin A and E and the content of fat (R2 = 0.57 and 0.75, respectively). The year did not have any statistically significant influence on the content of monitored vitamins. The content of both vitamins is dependent on the phase of lactation. The levels of vitamins A and E were significantly lower in the early phase and significantly higher in the late phase of lactation. The amount of monitored vitamins slightly decreased during pasteurisation. A strong decrease in the content of both vitamins was observed during the first two weeks after milk storage in a freezing box at the temperature of -20°C (about 11-55%). Source


Hejtmankova A.,Czech University of Life Sciences | Pivec V.,Czech University of Life Sciences | Trnkova E.,Czech University of Life Sciences | Dragounova H.,Dairy Research Institute Ltd.
Czech Journal of Animal Science | Year: 2012

This study was conducted to evaluate changes in composition of whey proteins of Czech White Short-haired goat and East Friesian ewe milk and their comparison throughout lactation. Some differences in composition between ewe and goat milk were found. The results showed that the mean total protein (%), whey protein (g/100 g), and β-lactoglobulin (β-Lg, g/100 g) contents of goat milk were 2.75, 0.433, and 0.119 respectively and of ewe milk 6.36, 1.11, and 0.732 respectively. The contents of total protein as well as acid whey proteins in goat milk were nearly constant throughout the lactation period and fluctuated around the mean value while the contents of total protein as well as acid whey proteins in ovine milk were dependent on the period of lactation. The total protein content in ovine milk continuously increased during the lactation period. A higher content of ovine acid whey proteins was noticed at the beginning and in the final period of lactation. The average ratio of whey to total protein was 15.8 ± 2.61% in goat milk and 17.4 ± 2.68% in ewe milk and ranged from 13.0 to 20.4% in goat and from 14.0 to 20.8% in ewe milk. The total contents of two major whey proteins. ?-lactalbumin and β-lactoglobulin (α-La + β-Lg = AG), averaged 87% of total whey protein, 92% in ovine milk. The main component of acid whey proteins in goat milk was ?-La while in ovine milk the main component of acid whey proteins was β-Lg, however, at the end of the lactation period the content of β-Lg for both kinds of milk increased steeply, and the β-Lg/α-La ratio reached a maximum value of 1.94 in goat milk and of 9.74 in ewe milk. In addition, goat milk contains a similar amino acid profile to ewe milk but the amino acid pattern in whey proteins differs from that in milk. Total essential amino acids were approximately 40% of the total amino acids in goat and ewe milk as well as in goat and ewe whey. Source


Lisova I.,Dairy Research Institute Ltd. | Horackova S.,Institute of Chemical Technology Prague | Kovacova R.,Institute of Chemical Technology Prague | Rada V.,Czech University of Life Sciences | Plockova M.,Institute of Chemical Technology Prague
Czech Journal of Food Sciences | Year: 2013

The commercial probiotic strain Bifidobacterium animalis subsp. lactis Bb12 was encapsulated using emulsion encapsulation into milk protein matrix without and with the addition of 0.5% w/w lecithin into the oil. Different agitation speeds were used during the encapsulation process. The examination of microcapsules was carried out by optical microscope and fluorescence in situ hybridisation. The particle size distribution as volume based median d 0.5 was evaluated by the laser diffraction method. In the case of no lecithin addition, the agitation speed did not influence significantly the size of the microcapsules. The addition of 0.5% (w/w) of lecithin into the oil caused a decrease of d0.5 value from 196 ± 37 μm to 79 ± 3 μm at an agitation speed of 500 rpm, and from 193 ± 24 μm to 39 ± 3 μm at 1200 rpm. It can improve the sensory properties of the products with the added microcapsules. Source

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