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Xia F.,Dahua Hospital of Xuhui District | Liu G.-H.,Shanghai JiaoTong University | Ouyang Q.,Shanghai JiaoTong University | Zhang Y.,Dahua Hospital of Xuhui District | And 4 more authors.
Chinese Journal of Interventional Imaging and Therapy | Year: 2015

Objective: To compare the difference in in-stent restenosis (ISR) between the coronary heart disease (CHD) with diabetes mellitus (DM) and simplex CHD following coronary stent implantation through the introduction of propensity score matching (PSM). Methods: The analysis was based on a retrospectively data on patients who underwent coronary stent implantation patients. Among them, CHD with DM (CHD+DM group) patients were 137 cases, simplex CHD (CHD group) patients were 283 cases. The two groups covariate were balanced based on the PSM function of SPSS software using 1:1 nearest neighbor matching method. The Cox Proportional hazards models were applied to identify the independent risk factors for ISR after PSM. Results: One hundred and twenty pairs of patients were successfully matched. Cox proportional hazard model by multivariate analysis found that history of smoking (HR=2.50, 95%CI: 1.34-4.64, P=0.004), history of hypertension (HR=2.24, 95%CI: 1.08-4.63, P=0.030), creatinine clearance rate<110 ml/min (HR=3.12, 95%CI: 1.22-5.03, P=0.024), multiple lesions of coronary artery (HR=2.15, 95%CI: 1.14-4.07, P=0.018), and DM (HR=2.22, 95%CI: 1.14-4.33, P=0.020) were independent risk factors for ISR in CHD patients. The 1, 3, and 5 years cumulative ISR rates were 10.70%, 40.30% and 43.80% in the CHD+DM group, and 4.70%, 23.70% and 29.60% in the CHD group, respectively. There were statistically significant differences between the two groups (P=0.023). Conclusion: The application of PSM can effectively balanced the covariates of groups in non-randomised study, DM is a risk factor for ISR for the CHD patients following coronary stent implantation. Copyright © 2015 by the Press of Chinese Journal of Medical Imaging and Technology.


Hu Q.,Dahua Hospital of Xuhui District | Zhou L.-H.,Shanghai University of Traditional Chinese Medicine | Liu X.,Shanghai University of Traditional Chinese Medicine | Sui H.,Shanghai University of Traditional Chinese Medicine | And 4 more authors.
World Chinese Journal of Digestology | Year: 2014

Aim: To study the effects of overexpression of homeodomain-interacting protein kinase 2 (HIPK2) on the expression of cyclooxygenase 2 (COX-2) and β-catenin in HCT-116 cells, and to explore the regulatory effect of the HIPK2 gene on angiogenic factor expression in human colorectal cancer cells. Methods: The pEGFP-N3 vector or pEGFPN3- HIPK2 plasmid was transfected into human colorectal cancer HCT-116 cells using Lipofectamine 2000, and untransfected cells were used as normal controls. The expression of VEGF (vascular endothelial growth factor) in cell culture medium was detected by ELISA assay, and the protein expression of β-catenin was tested by Western blot. The COX-2 promoter containing plasmid pGL3- basic-COX-2 was co-transfected with pRL-SV40 in the three groups above. COX-2 promoter activity was detected by dual luciferase activity assay, and COX-2 mRNA level was determined by real-time PCR. Results: The pEGFP-N3-HIPK2 group and pEGFP-N3 group had the same fluorescent level, and there was no statistically significant difference in HCT-116 cells. The VEGF expression was significantly inhibited (857.54 pg/mL ± 65.04 pg/mL vs 368.32 pg/mL ± 98.82 pg/mL, P < 0.05) after pEGFP-N3-HIPK2 plasmid transfection. COX-2 promoter activity and mRNA expression were significantly lower in the pEGFPN3- HIPK2 group than in the pEGFP-N3 group (75.467×10-5 ± 18.666×10-5 vs 266.407×10-5 ± 40.902 ×10-5, 1.07×10-4 ± 0.32×10-4 vs 3.48×10-4 ± 0.64×10-4 P < 0.05 for both). HIPK2 could inhibit the protein expression of β-catenin but up-regulate p-β- catenin expression. Conclusion: pEGFP-N3-HIPK2 transfection significantly increased the expression of HIPK2, and reduced the transcription of the COX2 gene and VEGF level in human colorectal cancer HCT-116 cells. The mechanisms may be related to the inhibition of COX-2 and β-catenin expression in cells, and regulation of β-catenin-COX-2 signal transduction. © 2014 Baishideng Publishing Group Co., Limited. All rights reserved.


PubMed | Dahua Hospital of Xuhui District, Tongji University and Shanghai JiaoTong University
Type: Journal Article | Journal: Molecular medicine reports | Year: 2016

Angiopoietin-like protein4 (Angptl4) is a secreted protein predominantly expressed in liver and adipose tissues, and has been identified as an adipokine. Angptl4 is the target gene of peroxisome proliferatoractivated receptors, which are widely used as lipidlowering and antidiabetic drugs, and previous studies have demonstrated that Angptl4 is able to directly stimulate adipocyte lipolysis. The current study focused on how Angptl4 was involved in regulating lipid and glucose metabolism inhighfatdiet (HFD) C57 mice. In the present study, mice were divided into three groups, with standard chow mice as a normal control, adenovirus (adv)injected HFD mice as a model control and advAngptl4injected HFD mice as the Angptl4+ group. Firstly, compared with the normal control group, mice in the model control group gained more body weight with severe liver steatosis and increased serum levels of triglyceride, total cholesterol, free fatty acids, alanine aminotransferase and aspartate aminotransferase. In the Angptl4+ group, Angptl4 reduced the weight growth rate, aggravated hepatic steatosis and further increased all the aforementioned serum indexes. Secondly, compared with the normal control, the model control group had a reduced glucose tolerance and developed insulin resistance. Angptl4 expression and the phosphorylation levels of several insulin signaling pathwayassociated genes, insulin receptor substrate1, protein kinaseB, janus kinase2, signal transducer and activator of transcription3 were downregulated in the liver samples. AdvAngptl4 injection was observed to improve glucose tolerance and insulin resistance. The genes measured were identified to be upregulated close to normal levels. All the results suggested that Angptl4 served an important role in lipid and glucose metabolism in HFDinduced obese mice, and this may have a great significance for treatment of hyperlipidemia, diabetes, metabolic syndrome and other diseases.


Cao J.,Dahua Hospital of Xuhui District | Liu H.-Q.,Dahua Hospital of Xuhui District | He Y.,Dahua Hospital of Xuhui District | Xia N.,Dahua Hospital of Xuhui District | And 2 more authors.
Journal of Interventional Radiology | Year: 2011

Objective: To investigate the clinical effect of metallic stent implantation together with intra-arterial chemotherapy in treating malignant gastric and duodenal obstruction. Methods: A total of 32 patients with malignant gastric and duodenal obstruction were enrolled in this study. The obstructed sites were located at the gastric sinus and pylorus part (n = 16), at the gastroduodenal anastomotic stoma (n = 6) or at the descending part of duodenum (n = 10). Under DSA guidance and with the additional help of endoscopy, a guide-wire was orally placed in the gastroduodenal obstructed site, which was followed by the implantation of the self-expanding metallic stent (Ni-Ti alloy). Postoperative intra-arterial chemotherapy via the tumor-feeding arteries was carried out in 16 patients (dual interventional therapy). The clinical results were analyzed. Results: Successful stent insertion was achieved in all 32 patients (100%). After stent implantation the obstructive symptoms were markedly relieved and the food intake was improved. No serious complications occurred. The median survival time for the 16 patients who had received dual interventional therapy was 9.3 months, while the median survival time for the other 16 patients who had received simple stenting therapy was 5.7 months. Conclusion: For the treatment of inoperable malignant gastroduodenal obstruction, the implantation of metallic self-expanding stents is a technically simple, clinically safe and effective palliative measure. Combined with postoperative intra-arterial chemotherapy, the metal stent implantation can control the tumor growth and elongate the survival time.


Yuan Y.,Dahua Hospital of Xuhui District | Qu B.,Dahua Hospital of Xuhui District | Yan J.,Dahua Hospital of Xuhui District | Wang H.,Dahua Hospital of Xuhui District | And 2 more authors.
Panminerva medica | Year: 2015

AIM: Many studies have reported detection of aberrant methylation of genes in stool sample for early colorectal cancer (CRC) or adenomas had high sensitivity and specificity, but still remained controversial. We aimed to evaluate the diagnostic value of stool testing for markers of CRC and adenomas.METHODS: Medline, EMbase, Web of Science, the Cochrane Library, and EMbase were systematically searched. Meta-analysis was performed using a random effects model with sensitivity, specificity, diagnostic OR (DOR), area under the summary ROC curves (SROC) and their 95% confidence interval (95% CI).RESULTS: A total of 13 studies including 716 patients with CRC 220 cases of adenoma and 414 healthy controls were eligible for final analysis. The sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, and DOR for the detection of CRC were 0.78 (95% CI: 0.75-0.82), 0.90 (95% CI: 0.91-0.96), 9.612 (95% CI: 6.761-13.666), 0.243 (95% CI: 0.176-0.336), and 48.21 (95% CI: 28.24-82.30), respectively. The sensitivity and specificity for the detection of adenoma were 0.63 (95% CI: 0.56-0.69) and 0.93 (95% CI: 0.90-0.96), respectively. The area under the SROC curve was 0.9438 and 0.9385 in CRC and adenoma detection, respectively.CONCLUSION: Fecal gene methylation testing as a noninvasive method can be used as a screening measurement for CRC or colorectal adenoma with high sensitivity and specificity.


Wang X.-R.,Fudan University | Wang X.-R.,Dahua Hospital of Xuhui District | Li S.-Q.,Fudan University
Fudan University Journal of Medical Sciences | Year: 2015

Objective: To evaluate the death influential factors of elderly patients with community acquired pneumonia (CAP) in a basic-level hospital during hospitalization. Methods: We collected 125 cases of elderly patients with CAP from respiratory ward in Dahua Hospital of Xuhui District of Shanghai during Jan., 2012 to Apr., 2015. They were divided into death group (19 cases) and survival group (106 cases) according to the survival situation on discharge. General data (age, basic diseases mergers, body mass index, hemoglobin, serum albumin levels, T cell subgroup), pneumonia severity (PSI, CURB-65 and APACHE II scores) of the two groups were compared and analyzed by statistical software. Results: Compared with survival group, the death group had advanced age, lower level of hemoglobin, serum albumin, CD3+T cell count, CD4+T cell count and CD8+T cell count significantly (P<0.05); Meanwhile, their pneumonia severity score (PSI, CURB-65 and APACHE II scores) increased significantly (P<0.05), which significantly associated with death (P<0.01); While basic diseases mergers or CD4+/CD8+ had no significant correlation with death (P>0.05). Significant negative correlation was found between advanced age and the level of hemoglobin and serum albumin levels. Significant positive correlation was also found between advanced age and PSI score, CURB-65 score and APACHE II score (P<0.01). But no significant correlation was found between advanced age and CD3+ T cell count or CD4+ T cell count. Significant negative correlation was found between the level of hemoglobin, serum albumin levels and PSI score, CURB-65 score and APACHE II score (P<0.01). Significant positive correlation was also found between serum albumin levels and CD3+ T cell count and CD4+ T cell count (P<0.01). Positive correlation was found between the level of hemoglobin and CD3+ T cell count or CD4+T cell count (P<0.05). Significant negative correlation was found between CD3+ T cell count or CD4+ T cell count and PSI score or APACHE II score (P<0.01), negative correlation was found between CD3+ T cell count and CURB-65 score (P<0.05), CD4+ T cell count and CURB-65 score had no significant correlation (P>0.05). Negative correlation was found between body mass index and death (P<0.05). Conclusions: Advanced age, malnutrition, impaired immune function and pneumonia illness severity were death risk factors of in-hospital elderly CAP patients, which should be given high attention. Positive anti-infection and other symptomatic treatment, improving patients' nutritional status, and immune adjustment to reduce the elderly hospitalized mortality rate of CAP patients are very important. © 2015, Editorial Department of Fudan University Journal of Medical Sciences. All right reserved


PubMed | Dahua Hospital of Xuhui District
Type: Journal Article | Journal: Panminerva medica | Year: 2015

Many studies have reported detection of aberrant methylation of genes in stool sample for early colorectal cancer (CRC) or adenomas had high sensitivity and specificity, but still remained controversial. We aimed to evaluate the diagnostic value of stool testing for markers of CRC and adenomas.Medline, EMbase, Web of Science, the Cochrane Library, and EMbase were systematically searched. Meta-analysis was performed using a random effects model with sensitivity, specificity, diagnostic OR (DOR), area under the summary ROC curves (SROC) and their 95% confidence interval (95% CI).A total of 13 studies including 716 patients with CRC 220 cases of adenoma and 414 healthy controls were eligible for final analysis. The sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, and DOR for the detection of CRC were 0.78 (95% CI: 0.75-0.82), 0.90 (95% CI: 0.91-0.96), 9.612 (95% CI: 6.761-13.666), 0.243 (95% CI: 0.176-0.336), and 48.21 (95% CI: 28.24-82.30), respectively. The sensitivity and specificity for the detection of adenoma were 0.63 (95% CI: 0.56-0.69) and 0.93 (95% CI: 0.90-0.96), respectively. The area under the SROC curve was 0.9438 and 0.9385 in CRC and adenoma detection, respectively.Fecal gene methylation testing as a noninvasive method can be used as a screening measurement for CRC or colorectal adenoma with high sensitivity and specificity.

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