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Hu Q.,Dahua Hospital of Xuhui District | Zhou L.-H.,Shanghai University of Traditional Chinese Medicine | Liu X.,Shanghai University of Traditional Chinese Medicine | Sui H.,Shanghai University of Traditional Chinese Medicine | And 4 more authors.
World Chinese Journal of Digestology | Year: 2014

Aim: To study the effects of overexpression of homeodomain-interacting protein kinase 2 (HIPK2) on the expression of cyclooxygenase 2 (COX-2) and β-catenin in HCT-116 cells, and to explore the regulatory effect of the HIPK2 gene on angiogenic factor expression in human colorectal cancer cells. Methods: The pEGFP-N3 vector or pEGFPN3- HIPK2 plasmid was transfected into human colorectal cancer HCT-116 cells using Lipofectamine 2000, and untransfected cells were used as normal controls. The expression of VEGF (vascular endothelial growth factor) in cell culture medium was detected by ELISA assay, and the protein expression of β-catenin was tested by Western blot. The COX-2 promoter containing plasmid pGL3- basic-COX-2 was co-transfected with pRL-SV40 in the three groups above. COX-2 promoter activity was detected by dual luciferase activity assay, and COX-2 mRNA level was determined by real-time PCR. Results: The pEGFP-N3-HIPK2 group and pEGFP-N3 group had the same fluorescent level, and there was no statistically significant difference in HCT-116 cells. The VEGF expression was significantly inhibited (857.54 pg/mL ± 65.04 pg/mL vs 368.32 pg/mL ± 98.82 pg/mL, P < 0.05) after pEGFP-N3-HIPK2 plasmid transfection. COX-2 promoter activity and mRNA expression were significantly lower in the pEGFPN3- HIPK2 group than in the pEGFP-N3 group (75.467×10-5 ± 18.666×10-5 vs 266.407×10-5 ± 40.902 ×10-5, 1.07×10-4 ± 0.32×10-4 vs 3.48×10-4 ± 0.64×10-4 P < 0.05 for both). HIPK2 could inhibit the protein expression of β-catenin but up-regulate p-β- catenin expression. Conclusion: pEGFP-N3-HIPK2 transfection significantly increased the expression of HIPK2, and reduced the transcription of the COX2 gene and VEGF level in human colorectal cancer HCT-116 cells. The mechanisms may be related to the inhibition of COX-2 and β-catenin expression in cells, and regulation of β-catenin-COX-2 signal transduction. © 2014 Baishideng Publishing Group Co., Limited. All rights reserved.

Cao J.,Dahua Hospital of Xuhui District | Liu H.-Q.,Dahua Hospital of Xuhui District | He Y.,Dahua Hospital of Xuhui District | Xia N.,Dahua Hospital of Xuhui District | And 2 more authors.
Journal of Interventional Radiology | Year: 2011

Objective: To investigate the clinical effect of metallic stent implantation together with intra-arterial chemotherapy in treating malignant gastric and duodenal obstruction. Methods: A total of 32 patients with malignant gastric and duodenal obstruction were enrolled in this study. The obstructed sites were located at the gastric sinus and pylorus part (n = 16), at the gastroduodenal anastomotic stoma (n = 6) or at the descending part of duodenum (n = 10). Under DSA guidance and with the additional help of endoscopy, a guide-wire was orally placed in the gastroduodenal obstructed site, which was followed by the implantation of the self-expanding metallic stent (Ni-Ti alloy). Postoperative intra-arterial chemotherapy via the tumor-feeding arteries was carried out in 16 patients (dual interventional therapy). The clinical results were analyzed. Results: Successful stent insertion was achieved in all 32 patients (100%). After stent implantation the obstructive symptoms were markedly relieved and the food intake was improved. No serious complications occurred. The median survival time for the 16 patients who had received dual interventional therapy was 9.3 months, while the median survival time for the other 16 patients who had received simple stenting therapy was 5.7 months. Conclusion: For the treatment of inoperable malignant gastroduodenal obstruction, the implantation of metallic self-expanding stents is a technically simple, clinically safe and effective palliative measure. Combined with postoperative intra-arterial chemotherapy, the metal stent implantation can control the tumor growth and elongate the survival time.

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