Ko K.-C.,Korea Research Institute of Bioscience and Biotechnology |
Lee J.H.,Korea Research Institute of Bioscience and Biotechnology |
Lee J.H.,Daesang Co. |
Han Y.,Korea Research Institute of Bioscience and Biotechnology |
And 2 more authors.
Biochemical and Biophysical Research Communications | Year: 2013
Metagenomic resources representing ruminal bacteria were screened for novel exocellulases using a robotic, high-throughput screening system, the novel CelEx-BR12 gene was identified and the predicted CelEx-BR12 protein was characterized. The CelEx-BR12 gene had an open reading frame (ORF) of 1140 base pairs that encoded a 380-amino-acid-protein with a predicted molecular mass of 41.8kDa. The amino acid sequence was 83% identical to that of a family 5 glycosyl hydrolase from Prevotella ruminicola 23. Codon-optimized CelEx-BR12 was overexpressed in Escherichia coli and purified using Ni-NTA affinity chromatography. The Michaelis-Menten constant (Km value) and maximal reaction velocity (Vmax values) for exocellulase activity were 12.92μM and 1.55×10-4μmolmin-1, respectively, and the enzyme was optimally active at pH 5.0 and 37°C. Multifunctional activities were observed against fluorogenic and natural glycosides, such as 4-methylumbelliferyl-β-d-cellobioside (0.3Umg-1), CMC (105.9Umg-1), birch wood xylan (132.3Umg-1), oat spelt xylan (67.9Umg-1), and 2-hydroxyethyl-cellulose (26.3Umg-1). Based on these findings, we believe that CelEx-BR12 is an efficient multifunctional enzyme as endocellulase/exocellulase/xylanase activities that may prove useful for biotechnological applications. © 2013 Elsevier Inc.
Daesang Corporation | Date: 2012-08-10
An exemplary embodiment of the present invention provides starch-based hot melt adhesive comprised of a composition including starch, a thermoplastic polymer, a tackifier, a plasticizer, and an adhesion promoter. The hot melt adhesive according to the exemplary embodiment of the present invention contains about 25 to 55 weight % of the starch which is infinitely renewable biomass, and, thus, it is less harmful and more eco-friendly and has a reduced manufacturing costs and is less sensitive to exhaustion of petroleum-based resources, as compared with the conventional hot melt adhesive mainly containing a petroleum-based material. Further, the hot melt adhesive according to the exemplary embodiment of the present invention imparts excellent adhesion strength and workability at the time of adhesion between different kinds of adherends such as a hydrophobic adherend and a hydrophilic adherend and can be applied to various fields such as packaging, bookbinding, construction, woodworking, and textile fields.
DAESANG Corporation | Date: 2016-04-12
Fertilizers; industrial chemicals; chemicals preparations for industrial or scientific purposes; adhesives for industrial purposes; artificial sweeteners; plant growth regulators; microorganisms other than medical purposes; chemical test paper; unprocessed artificial resins; rare earths; antifreeze. Food for babies, except lacteal flour for babies; milk sugar for pharmaceutical purposes; dietary fiber; lysine preparations; starch for dietetic or pharmaceutical purposes; vitamin preparations; mineral food supplements; nutritional supplements; microorganisms for medical purposes; cotton for medical purposes; menstruation bandages; diapers for babies.
Daesang Corporation | Date: 2012-09-06
There is provided a method of preparing resistant starch with improved processability as one aspect of the present invention, the method comprising: (a) preparing starch modified by a cross-linked bond; and (b) feeding the modified starch to an extruder and then extruding the modified starch to produce an extrudate, in which an extrusion temperature is 50 C. to 200 C. at the time of the extrusion, the ratio of a injection volume of water to a supplying amount of the modified starch, each being supplied into the extruder, is 1.2 ml/g to 2.0 ml/g, and water content in the extrudate is 25 wt % to 50 wt %.
Daesang Corporation | Date: 2014-12-01
The present invention relates to a complex amino acid seasoning composition, comprising L-glutamic acid and L-lysine; wherein the composition is in the amorphous form and exhibits pH 6.5-8.0 in an aqueous solution. In addition, the present invention relates to a preparation method of the complex amino acid seasoning composition. The present invention provides amino acid seasoning compositions with excellent sensory properties in terms of saltiness and umami by use of L-glutamic acid and L-lysine. In contrast to conventional technologies, the present invention enables to block bitterness and offensive odor originated from amino acid ingredients, providing health-friendly seasoning compositions with no sodium ingredient. The seasoning composition of the present invention can be prepared with no formation of L-glutamic acid.L-lysine crystals in a very convenient and cost-effective manner.