Miaoli, Taiwan
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Chen S.-C.,Da Chien General Hospital | Chen S.-C.,University of Taipei | Tan L.-B.,Gods Help Hospital | Huang L.-M.,National Taiwan University Hospital | Chen K.-T.,National Cheng Kung University
Journal of the Formosan Medical Association | Year: 2012

Among children, rotaviruses are the most common cause of severe gastroenteritis worldwide and of diarrheal deaths in developing countries. Current vaccines (e.g., Rotarix, GlaxoSmithKline Biologicals; RotaTeq, Merck and Company) effectively reduce rotaviral gastroenteritis, emergency department visits, and hospitalizations. The tremendous burden of rotavirus-related diarrhea in children across the world continues to drive the remarkable pace of vaccine development. This review assesses the global epidemiological and economic burden of rotavirus diseases, summarizes the relevant principles of the development of rotavirus vaccines, and presents data on the efficacy and effectiveness of currently licensed vaccines in both developed and developing countries. © 2012.


Tsai C.-T.,Da Chien General Hospital | Hsieh L.-F.,Fu Jen Catholic University | Kuan T.-S.,National Cheng Kung University | Kao M.-J.,Taipei Medical University Hospital | And 2 more authors.
American Journal of Physical Medicine and Rehabilitation | Year: 2010

Objective: To investigate the remote effect of dry needling on the irritability of a myofascial trigger point in the upper trapezius muscle. Design: Thirty-five patients with active myofascial trigger points in upper trapezius muscles were randomly divided into two groups: 18 patients in the control group received sham needling, and 17 patients in the dry-needling group received dry needling into the myofascial trigger point in the extensor carpi radialis longus muscle. The subjective pain intensity, pressure pain threshold, and range of motion of the neck were assessed before and immediately after the treatment. Results: Immediately after dry needling in the experimental group, the mean pain intensity was significantly reduced, but the mean pressure threshold and the mean range of motion of cervical spine were significantly increased. There were significantly larger changes in all three parameters of measurement in the dry-needling group than that in the control group. Conclusions: This study demonstrated the remote effectiveness of dry needling. Dry needling of a distal myofascial trigger point can provide a remote effect to reduce the irritability of a proximal myofascial trigger point. © 2009 by Lippincott Williams & Wilkins.


Chang W.-D.,China Medical University at Taichung | Chen F.-C.,National Pingtung University of Science and Technology | Lee C.-L.,National Sun Yat - sen University | Lin H.-Y.,I - Shou University | Lai P.-T.,Da Chien General Hospital
Evidence-based Complementary and Alternative Medicine | Year: 2015

Objectives. To conduct a systematic review comparing the effects of Kinesio taping with McConnell taping as a method of conservative management of patients with patellofemoral pain syndrome (PFPS). Methods. MEDLINE, PUBMED, EMBASE, AMED, and the Cochrane Central Register of Control Trials electronic databases were searched through July 2014. Controlled studies evaluating the effects of Kinesio or McConnell taping in PFPS patients were retrieved. Results. Ninety-one articles were selected from the articles that were retrieved from the databases, and 11 articles were included in the analysis. The methods, evaluations, and results of the articles were collected, and the outcomes of patellar tapings were analyzed. Kinesio taping can reduce pain and increase the muscular flexibility of PFPS patients, and McConnell taping also had effect in pain relief and patellar alignment. Meta-analysis showed small effect in pain reduction and motor function improvement and moderate effect in muscle activity change among PFPS patients using Kinesio taping. Conclusions. Kinesio taping technique used for muscles can relieve pain but cannot change patellar alignment, unlike McConnell taping. Both patellar tapings are used differently for PFPS patients and substantially improve muscle activity, motor function, and quality of life. © 2015 Wen-Dien Chang et al.


Huang G.-J.,China Medical University at Taichung | Pan C.-H.,China Medical University at Taichung | Liu F.-C.,China Medical University at Taichung | Liu F.-C.,Da Chien General Hospital | And 3 more authors.
Food and Chemical Toxicology | Year: 2012

The purpose of this study was to examine anti-inflammatory effect of ethanolic extract of Antrodia salmonea (EAS) in the lipopolysaccharide (LPS)-stimulated RAW246.7 macrophages and the carrageenan (Carr)-induced edema paw model, and to clarify its possible molecular mechanisms. Inhibitory effects of EAS were examined on cells proliferation, nitric oxide (NO) production, expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins, and activity of antioxidant enzymes. Our data demonstrated that EAS inhibited cell growth, NO production, and expression of iNOS and COX-2 proteins in LPS-stimulated RAW246.7 cells. EAS can also significantly reduce paw edema, content of NO, TNF-α and malondialdehyde (MDA), expression of iNOS and COX-2 proteins, and neutrophil infiltration within the tissues stimulated by Carr. The anti-inflammatory mechanisms of EAS might be related to the decrease of inflammatory cytokine and increase of antioxidant enzymes activities, which would result in reduction of iNOS, COX-2 and MDA and subsequently inflammatory responses. © 2012 Elsevier Ltd.


Tang M.-B.,Wei Gong Memorial Hospital | Chen C.-H.,Tatung University | Chen S.-C.,Tatung University | Chen S.-C.,Da Chien General Hospital | And 2 more authors.
BMC Infectious Diseases | Year: 2013

Background: The human norovirus (NV) circulates worldwide and is a major cause of epidemics, which have increased in Taiwan since 2002. NV in acute gastroenteritis (AGE) and non-acute gastroenteritis (asymptomatic) patients, including children and adults, have not been previously examined in Taiwan; therefore, we examined the epidemiology and phylogeny of NV in AGE and asymptomatic patients of all ages.Methods: 253 stool samples were collected from August 2011 to July 2012 (including 155 AGE and 98 asymptomatic samples in Taiwan) and analyzed using reverse transcription-polymerase chain reaction (RT-PCR) for NV. Primers targeting the RNA-polymerase gene were used for RT-PCR to allow DNA sequencing of Taiwan NV strains and phylogenetic analyses.Results: NV was detected in 24 (9.5%) of 253 stool specimens using RT-PCR. NV was isolated from all age groups (1 to 86 y) and those NV-positive samples were major identified from inpatients (79.2%, 19/24). Statistical analysis showed that the NV infectious rate of AGE patients was statistically significant (P < 0.05) for age, season and water type, respectively. Phylogenetic analyses of the RdRp region sequence showed that 24 NV isolates belonged to Genogroup II Genotype 4 (GII.4). They were closely related to the epidemic strain in Taiwan in 2006, the GII.4-2006b pandemic strain in 2006, and the GII.4-New Orleans strain in 2010.Conclusion: This study is the first to examine NV in sporadic AGE and asymptomatic patients in Taiwan. Furthermore, epidemic strains of isolated GII.4 were predominant in Taiwan during 2011 and 2012. © 2013 Tang et al.; licensee BioMed Central Ltd.


Hsu P.-C.,National Chung Hsing University | Hsu P.-C.,Da Chien General Hospital | Liao Y.-F.,Chaoyang University of Technology | Lin C.-L.,Chung Shan Medical University | And 3 more authors.
Molecules and Cells | Year: 2014

Peptidylarginine deiminase type 2 (PADI2) deiminates (or citrullinates) arginine residues in protein to citrulline residues in a Ca2+- dependent manner, and is found in lymphocytes and macrophages. Vimentin is an intermediate filament protein and a well-known substrate of PADI2. Citrullinated vimentin is found in ionomycin-induced macrophage apoptosis. Citrullinated vimentin is the target of anti-Sa antibodies, which are specific to rheumatoid arthritis, and play a critical role in the pathogenesis of the disease. To investigate the role of PADI2 in apoptosis, we generated a Jurkat cell line that overexpressed the PADI2 transgene from a tetracycline-inducible promoter, and used a combination of 12-O-tetradecanoylphorbol-13-acetate and ionomycin to activate Jurkat cells. We found that PADI2 overexpression reduced the cell viability of activated Jurkat cells in1 a dose- and time-dependent manner. The PADI2-overexpressed and -activated Jurkat cells presented typical manifestations of apoptosis, and exhibited greater levels of citrullinated proteins, including citrullinated vimentin. Vimentin overexpression rescued a portion of the cells from apoptosis. In conclusion, PADI2 overexpression induces apoptosis in activated Jurkat cells. Vimentin is involved in PADI2-induced apoptosis. Moreover, PADI2-overexpressed Jurkat cells secreted greater levels of vimentin after activation, and expressed more vimentin on their cell surfaces when undergoing apoptosis. Through artificially highlighting PADI2 and vimentin, we demonstrated that PADI2 and vimentin participate in the apoptotic mechanisms of activated T lymphocytes. The secretion and surface expression of vimentin are possible ways of autoantigen presentation to the immune system. © The Korean Society for Molecular and Cellular Biology. All rights reserved.


Chang N.-Y.,Da Chien General Hospital | Chang N.-Y.,Taichung Veterans General Hospital | Wang J.,Taichung Veterans General Hospital | Wen M.-C.,Taichung Veterans General Hospital | Lee F.-Y.,Taichung Veterans General Hospital
International Journal of Surgical Pathology | Year: 2014

Langerhans cell sarcoma (LCS) is a rare malignancy requiring differential diagnosis from other high-grade nonhematologic and hematologic tumors. The pathogenesis of LCS remains unknown. Notably, LCS and its benign counterpart, Langerhans cell histiocytosis (LCH), are frequently associated with other malignancies. To the best of our knowledge, we describe the first case of LCS in a chronic myelogenous leukemia (CML) patient undergoing imatinib mesylate therapy. We performed molecular cytogenetic analyses for investigating the association between LCS and CML. In our case, molecular cytogenetic analysis did not reveal BCR-ABL1 fusion and BRAF V600E mutation, suggesting that LCS may be coincident in this patient. However, recurrent BRAF V600E mutation has been found in LCH. Published reports have revealed the clonal relationship between LCH/LCS and other hematologic malignancies, especially lymphoid neoplasms. However, there are only 2 reports demonstrating the clonal relationship between LCH and myeloid neoplasms. The association of LCH/LCS with myeloid neoplasms and the role of BRAF V600E mutation in LCS are discussed. © The Author(s) 2013.


Sun J.-R.,Tri Service General Hospital | Chan M.-C.,Tri Service General Hospital | Chang T.-Y.,Tri Service General Hospital | Wang W.-Y.,Tri Service General Hospital | And 2 more authors.
Antimicrobial Agents and Chemotherapy | Year: 2010

Thirteen clinical isolates of multidrug-resistant Acinetobacter baumannii resistant to carbapenems (MRAB-C) with tigecycline nonsusceptibility were collected from individual patients in this study. None of the 13 isolates shared the same strain characteristics in molecular typing. All of them showed increased adeB transcription, as predicted. However, none of these tigecycline-nonsusceptible MRAB-C isolates were found to possess previously known adeRS mutations. Upregulation of adeB transcription may result from cross stimulation by other mechanisms. Copyright © 2010, American Society for Microbiology. All Rights Reserved.


Chang W.-D.,China Medical University at Taichung | Lai P.-T.,Da Chien General Hospital | Tsou Y.-A.,China Medical University at Taichung
American Journal of Chinese Medicine | Year: 2014

Lateral epicondylalgia is a common orthopedic disorder. In traditional Chinese medicine, acupuncture is often used for treating lateral epicondylalgia. Laser acupuncture, compared with manual acupuncture, has more advantages because it is painless, aseptic and safe. However, the analgesic effect of manual acupuncture and laser acupuncture on lateral epicondylalgia has rarely been explored. We conducted a systematic review and meta-analysis to compare the analgesic effect of laser acupuncture and manual acupuncture for the treatment of lateral epicondylalgia. We investigated studies published in the Medline, PubMed, and CINAHL databases from January 1980 to December 2013. This review included 9 randomized articles. Six of them examined manual acupuncture and the others focused on laser acupuncture. We analyzed the meta-analysis results regarding the analgesic effect of the treatment, and observed substantial differences in 4 articles related to manual acupuncture. Manual acupuncture is effective in short-term pain relief for the treatment of lateral epicondylalgia; however, its long-term analgesic effect is unremarkable. A suitable acupuncture point and depth can be used to treat lateral epicondylalgia. Manual acupuncture applied on lateral epicondylalgia produced stronger evidence of an analgesic effect than did laser acupuncture, and further study on the analgesic effect of laser acupuncture is required. © 2014 World Scientific Publishing Company & Institute for Advanced Research in Asian Science and Medicine.


Tsai C.-T.,Da Chien General Hospital | Chang W.-D.,Da Chien General Hospital | Lee J.-P.,Da Chien General Hospital
Journal of Musculoskeletal Pain | Year: 2010

Objectives: The purpose of this study was to investigate the therapeutic effects of kinesiotaping on plantar fasciitis. Methods: A total of 52 patients with plantar fasciitis were randomly and equally divided into two groups. The patients in the control group received only a traditional physical therapy program daily, including ultrasound thermotherapy and low-frequency electrotherapy. The patients in the experimental group received kinesiotaping in addition to the same physical therapy program as the control group. The tape for kinesiotaping was applied on the gastrocnemius and the plantar fascia continuously for one week. For each patient, the therapeutic effects were measured with subjective pain assessment pain description scores and foot function scores and ultrasonographic assessment measuring plantar fascia thickness and structural change. The investigators who performed the assessment were blinded as to the group assignment of the subject. Results: The reduced pain scores pain description scores and foot function scores and the reduced thickness of plantar fascia at the insertion site ultrasound assessment after treatment were significantly p < 0.05 more in the experimental group than in the control group. However, there were no significant p > 0.05 differences in the changes of plantar fascia thickness at the site 0.5 cm distal to the insertion site and hypoechoic phenomena. Conclusions: It was concluded that the additional treatment with continuous kinesiotaping for one week might alleviate the pain of plantar fasciitis better than a traditional physical therapy program only. Copyright © 2010 Informa Healthcare USA, Inc.

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