Auburn University, AL, United States
Auburn University, AL, United States

Time filter

Source Type

Zarogoulidis P.,Aristotle University of Thessaloniki | Zarogoulidis P.,University of Duisburg - Essen | Hohenforst-Schmidt W.,University of Würzburg | Darwiche K.,University of Duisburg - Essen | And 8 more authors.
Gene Therapy | Year: 2013

Revealing the lung tumor genome has directed the current treatment strategies toward targeted therapy. First line treatments targeting the genome of lung tumor cells have been approved and are on the market. However, they are limited by the small number of patients with the current investigated genetic mutations. Novel treatment administration modalities have been also investigated in an effort to increase the local drug deposition and disease control. In the current study, we investigated the safety of the new nonviral vector 2-diethylaminoethyl-dextran methyl methacrylate copolymer (DDMC; Ryujyu Science), which belongs to the 2-diethylaminoethyl-dextran family by aerosol administration. Thirty male BALBC mice, 2 month old, were included and divided into three groups. However, pathological findings indicated severe emphysema within three aerosol sessions. In addition, the CytoViva technique was applied for the first time to display the nonviral particles within the pulmonary tissue and emphysema lesions, and a spectral library of the nonviral vector was also established. Although our results in BALBC mice prevented us from further investigation of the DDMC nonviral vector as a vehicle for gene therapy, further investigation in animals with larger airways is warranted to properly evaluate the safety of the vector. © 2013 Macmillan Publishers Limited All rights reserved.

Misra S.K.,University of Illinois at Urbana - Champaign | Ostadhossein F.,University of Illinois at Urbana - Champaign | Daza E.,University of Illinois at Urbana - Champaign | Johnson E.V.,CytoViva | Pan D.,University of Illinois at Urbana - Champaign
Advanced Functional Materials | Year: 2016

Spatial and spectral information of a nanocarrier and its payload is crucial for the advancement of luminescence-based imaging, disease detection, and treatment in complex biological environment. However, it remains challenging to track and quantify the delivery and localization of drugs lacking inherent fluorescence. It is demonstrated that sub 30 nm phospholipid-stabilized nanoparticles can be detected and quantified using hyperspectral transmitted light microscopy without using a fluorophore. In two proposed model systems, phospholipid-passivated carbon nanoparticles incorporate the drug in either free form or as a lipid-based prodrug. Following a rigorous characterization of these nanoparticles, in vitro toxicities via loss in cell growth density and mitochondrial respiration is studied in MCF-7 breast cancer cells. Furthermore, a detailed inhibitor based study reveals that these particles are internalized based on a clathrin-mediated pathway, irrespective of the choice of drug formulation. Hyperspectral imaging is performed to obtain the colocalization of carbon nanoparticles and drug molecules intracellularly and can successfully be tracked while therapeutic release is quantified in 3D space. The present work demonstrates that nanoparticles and therapeutic agents can be mapped and measured simultaneously barring the requirement of a dye, thus providing new avenues in the spatiotemporal characterization and synchronous detection and quantification of payload and carrier. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA., Weinheim.

News Article | November 1, 2016
Site: adds “Global Hyperspectral Imaging Market 2016-2020” new report to its research database. The report spread across 62 pages with table and figures in it. The Research analysts forecast the global hyperspectral imaging market to grow at a CAGR of 11.59% during the period 2016-2020. Hyperspectral refers to the number of bands into which the spectrum is divided. Before HSI, multispectral imaging (which divides the spectrum into a few bands) was common. An HSI sensor is the key component for HSI. It converts the light received from objects into electrical signals. This is achieved with the help of the following components: - Lenses, which focus the incoming light - A slit, which limits the incoming light stream to a thin and wide beam - A diffraction grating, which disperses the beam into its spectra - Photo-receptors Browse full table of contents and data tables at Covered in this report The report covers the present scenario and the growth prospects of the global hyperspectral imaging market for 2016-2020. To calculate the market size, The Research considers the sale of HSI devices for end-user applications. The report does not include revenue generated from aftermarket service of products. The market is divided into the following segments based on geography: - APAC - Europe - North America - ROW The Research report, Global Hyperspectral Imaging (HSI) Market 2016-2020, has been prepared based on an in-depth market analysis with inputs from industry experts. The report covers the market landscape and its growth prospects over the coming years. The report also includes a discussion of the key vendors operating in this market. Key vendors - Headwall Photonics - IMEC - Norsk Electro Optikk AS - SPECIM - Telops Other prominent vendors - Applied Spectral Imaging - Brandywine Photonics - ChemImage - Corning - Corescan - Cubert - CytoViva - EVK DI Kerschhaggl - FluxData - Galileo Group - Gilden Photonics - Gooch & Housego - HyVista Corporation - ITRES - Merrick & Company - MicaSense - Resonon - SpecTIR - Surface Optics - Teledyne DALSA - XIMEA Market driver - Advanced medical imaging capabilities - For a full, detailed list, view our report Market challenge - Super-resolution challenges in HSI - For a full, detailed list, view our report Market trend - Emergence of handheld HSI cameras - For a full, detailed list, view our report Key questions answered in this report - What will the market size be in 2020 and what will the growth rate be? - What are the key market trends? - What is driving this market? - What are the challenges to market growth? - Who are the key vendors in this market space? - What are the market opportunities and threats faced by the key vendors? - What are the strengths and weaknesses of the key vendors? To receive personalized assistance write to us @ [email protected] with the report title in the subject line along with your questions or call us at +1 866-764-2150

Home > Press > SUNY Poly, in Collaboration with the George Washington School of Medicine and Health Sciences and Stony Brook University, Demonstrates Pioneering Method to Visualize and Identify Engineered Nanoparticles in Tissue Abstract: Research published in Microscopy Research and Technique details rapid, cost-effective hyperspectral imaging method for nanomaterial analysis that can shed light on nanomaterials’ health impact. As a testament to Governor Andrew Cuomo’s leadership in developing an unparalleled research and development ecosystem in New York State, Sara Brenner, MD, MPH and her colleagues at SUNY Polytechnic Institute (SUNY Poly), the George Washington School of Medicine and Health Sciences, and Stony Brook University have demonstrated a novel method for the rapid visualization and identification of engineered nanoparticles in tissues. This research, published in Microscopy Research and Technique (“Hyperspectral Imaging of Nanoparticles in Biological Samples: Simultaneous Visualization and Elemental Identification”), presents a method for utilizing enhanced darkfield microscopy (EDFM) and hyperspectral imaging (HSI) to easily and rapidly image nanoparticles in tissues from toxicology studies and map the distribution of nanoparticles throughout biological samples based on elemental composition. “As a result of Governor Andrew M. Cuomo’s visionary investments in New York State’s high-tech corridor, SUNY Poly is proud to be at the forefront of nanomaterials research where its cutting-edge resources can be leveraged to maximize our understanding of the true impact of these materials,” said Dr. Michael Liehr, SUNY Poly Executive Vice President of Innovation and Technology and Vice President of Research. “I commend Dr. Brenner and her research partners for demonstrating a more efficient evaluation technique, which could help to ensure workers and nano-based industry environments are kept as safe as possible.” As nanoparticles are increasingly incorporated into industrial processes and consumer products, studying the potential effects of exposure is critical to ensure the health and safety of workers, consumers, and the environment. In particular, the semiconductor industry utilizes metal oxide nanoparticles in a fabrication process, which has been identified by the industry as a critical area for health and safety research due to the potential for worker exposure. In their recent publication, the researchers were able to detail how they located metal oxide nanoparticles in an ex vivo porcine skin tissue model of cutaneous exposure. “The current gold standard for visualization of nanoparticles in tissue samples is electron microscopy, which is highly time- and resource-intensive,” said Dr. Sara Brenner, Assistant Professor of Nanobioscience and Assistant Vice President for NanoHealth Initiatives at SUNY Poly and corresponding author of the study. “Availability of an alternative, rapid, and cost-effective method would relieve this analytical bottleneck, not only in nanotoxicology, but in many fields where nanoscale visualization is critical. New and emerging analytical methods and tools for nanomaterial detection, visualization, and characterization must keep pace with innovation in terms of nanomaterial development, use, and commercialization. Therefore, forms of higher-throughput screening and direct visualization technology, such as this one, must be leveraged for studying not only nanomaterial behavior in biological systems, but also applied in the context of exposure assessment. The system has great versatility and high practical utility – we’ve only begun to scratch the surface of what it can do,” said Dr. Brenner. The research team utilized CytoViva, Inc.’s HSI system, which incorporates an enhanced darkfield microscope that has improved contrast and a high signal-to-noise ratio for easy visualization of nanoparticles, as well as a hyperspectral imaging camera, which combines spectrophotometry and imaging, using advanced optics and algorithms to capture a spectrum from 400-1,000nm at each pixel in a hyperspectral image. Hyperspectral data can then be used to identify materials of interest in a sample without the need for fluorescent labeling or other destructive sample preparation techniques. “Nanomaterials have been used for decades in the dermatology consumer space, ranging from sunscreens to anti-aging cosmetics to antimicrobial dressings. Our ability to dispel concerns regarding safety has been limited due to the constraints of our imaging approaches, which is why the publication of this now validated technique is so important,” said Dr. Adam Friedman, Associate Professor of Dermatology and Director of Translational Research at the George Washington School of Medicine and Health Sciences. “We are excited that Microscopy Research and Technique has recognized the significance of our joint research findings, which were made possible through this unique collaboration,” said Dr. Mary Frame, Stony Brook University Associate Professor and Distinguished Service Professor in the Biomedical Engineering department. “By laying the groundwork for the most efficient means with which to visualize nano materials in great detail, we are able to better evaluate the health implications of these particles as they come into contact with humans in the work environment and beyond, potentially paving the way for enhanced measures that can ensure health and safety.” “CytoViva’s state-of-the-art HSI system is a powerful tool for the visualization of nanoparticles, enabling high-throughput for the efficient attainment of critical health data,” said CytoViva, Inc. CEO Sam Lawrence. “We are thrilled to play a key role in this SUNY Poly-led high-tech public-private partnership that is breaking down previous research barriers to enable the data-driven, safer nano-based work environments of the future.” Not only did the researchers demonstrate the capability of EDFM-HSI to identify and map metal oxide nanoparticles in a porcine skin tissue model of exposure (Figure 1), but they also confirmed this method using traditional methods: Raman spectroscopy (RS) and scanning electron microscopy (SEM) with energy dispersive X-ray spectroscopy (EDS) for elemental analysis. After identifying areas within positive control tissue samples that were known to contain nanoparticles of interest, the same areas were analyzed via SEM-EDS and RS, which confirmed the identity of the materials. Once these areas were confirmed to be the nanoparticles of interest, reference spectral libraries (RSLs) containing hyperspectral data were created from these areas. RSLs were then used to map the experimental samples to assess the presence and distribution of nanoparticles in those tissues, using the spectral angle mapper (SAM) algorithm in the hyperspectral imaging and analysis software (ENVI 4.8). lthough EDFM-HSI has lower spatial resolution than electron microscopy, it confers several advantages over traditional electron microscopy and Raman spectroscopy methods, particularly in terms of time- and cost-reduction, making it an attractive alternative for the visualization and identification of nanoparticles. Since EDFM-HSI is useful for studying nanoparticle biodistribution, it can also be applied in areas of medical research, such as nanoscale drug delivery. The research team is expanding this work to other toxicological models of exposure, various nanomaterials, and other media types, including environmental and occupational exposure samples. In partnership with the National Institute for Occupational Safety and Health (NIOSH), EDFM-HSI methods are being developed to analyze air filters worn by workers who handle engineered nanomaterials, which reveals information about inhalation exposure in the workplace. Further work also continues on protocol development to assess nanoparticle penetration in cutaneous exposure models and nanoparticle quantitation in histological samples. Notably, this research represents one of many focus areas in the NanoHealth & Safety Consortium (NHSC), a SUNY Poly-based public-private platform linking human and environmental health and safety across industries to promote nanotechnology commercialization, which is a key component of a recently announced White House initiative. For more information, please click If you have a comment, please us. Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

More S.S.,University of Minnesota | Beach J.M.,CytoViva | Vince R.,University of Minnesota
Investigative Ophthalmology and Visual Science | Year: 2016

Purpose: To describe a spectral imaging system for small animal studies based on noninvasive endoscopy of the retina, and to present time-resolved spectral changes from live Alzheimer's mice prior to cognitive decline, corroborating our previous in vitro findings. Methods: Topical endoscope fundus imaging was modified to use a machine vision camera and tunable wavelength system for acquiring monochromatic images across the visible to near-infrared spectral range. Alzheimer's APP/PS1 mice and age-matched, wild-type mice were imaged monthly from months 3 through 8 to assess changes in the fundus reflection spectrum. Optical changes were fit to Rayleigh light scatter models as measures of amyloid aggregation. Results: Good quality spectral images of the central retina were obtained. Short-wavelength reflectance from Alzheimer's mice retinae showed significant reduction over time compared to wild-type mice. Optical changes were consistent with an increase in Rayleigh light scattering in neural retina due to soluble Aβ1–42 aggregates. The changes in light scatter showed a monotonic increase in soluble amyloid aggregates over a 6-month period, with significant build up occurring at 7 months. Conclusions: Hyperspectral imaging technique can be brought inexpensively to the study of retinal changes caused by Alzheimer's disease progression in live small animals. A similar previous finding of reduction in the light reflection over a range of wavelengths in isolated Alzheimer's mice retinae, was reproducible in the living Alzheimer's mice. The technique presented here has a potential for development as an early Alzheimer's retinal diagnostic test in humans, which will support the treatment outcome. © 2016, Association for Research in Vision and Ophthalmology Inc. All rights reserved.

Beach J.M.,CytoViva | Uertz J.L.,CytoViva | Eckhardt L.G.,Auburn University
Microscopy Research and Technique | Year: 2015

A new method of interferometry employing a Fabry-Perot etalon model was used to locate and size microscale features on the surface of the pine bark beetle. Oscillations in the reflected light spectrum, caused by self-interference of light reflecting from surfaces of foreleg setae and spores on the elytrum, were recorded using white light hyperspectral microscopy. By making the assumption that pairs of reflecting surfaces produce an etalon effect, the distance between surfaces could be determined from the oscillation frequency. Low frequencies of less than 0.08 nm-1 were observed in the spectrum below 700 nm while higher frequencies generally occupied wavelengths from 600 to 850 nm. In many cases, two frequencies appeared separately or in combination across the spectrum. The etalon model gave a mean spore size of 3.04±1.27 μm and a seta diameter of 5.44±2.88 μm. The tapering near the setae tip was detected as a lowering of frequency. Spatial fringes were observed together with spectral oscillations from surfaces on the exoskeleton at higher magnification. These signals were consistent with embedded multi-layer reflecting surfaces. Possible applications for hyperspectral interferometry include medical imaging, detection of spore loads in insects and other fungal carriers, wafer surface and subsurface inspection, nanoscale materials, biological surface analysis, and spectroscopy calibration. This is, to our knowledge, the first report of oscillations directly observed by microscopy in the reflected light spectra from Coleoptera, and the first demonstration of broadband hyperspectral interferometry using microscopy that does not employ an internal interferometer. © 2015 Wiley Periodicals, Inc.

CytoViva | Date: 2016-07-28

Microscope parts, namely, universal adapters to be used with microscopes that enable one to observe high contrast, high resolution images of living, fixed, and non-biological samples, optically imaging emulsions, and nano-materials; microscope parts, namely, universal modules used with a microscope that enable one to shift from a darkfield image to a fluorescent-stained image and a mode to see them both at the same time.

Ethylene-co-acrylic acid (EAA) and ethylene-co-methacrylic acid ionomer (EMAZ) copolymers were used as compatibilizers for polyethylene-graphene nanocomposites generated by melt mixing. At 5 wt% content, the EAA compatibilizer enhanced the tensile modulus of PE by 40 % and shear modulus by >300 % (1 rad/s) due to efficient dispersion of graphene platelets which helped in effective stress transfer. These also resulted in enhanced thermal stability for PE-EAA-G nanocomposite as compared to nanocomposite with EMAZ. The properties of the nanocomposites were significantly better than the conventional nanocomposites based on layered silicate materials. Mapping of the component distribution in the nanocomposites was demonstrated by using hyperspectral imaging. The nanocomposite with EAA exhibited higher extent of spectral signal mixing due to better mixing of filler and compatibilizer in PE matrix. On the other hand, nanocomposite with EMAZ had no spectral mixing as the components did not mix optimally with each other. The DSC thermogram for this nanocomposite also exhibited a small shoulder at low temperature probably due to immiscibility of the compatibilizer with the matrix polymer. The hyperspectral imaging and mapping was thus demonstrated to be a useful method for determination of component distribution in complex nanocomposite systems. © 2014, Springer-Verlag Berlin Heidelberg.

Disclosed are various embodiments for methods and systems for three-dimensional imaging of subject particles in media through use of dark-field microscopy. Some examples, among others, include a method for obtaining a three-dimensional (3D) volume image of a sample, a method for determining a 3D location of at least one subject particle within a sample, a method for determining at least one spatial correlation between a location of at least one subject particle and a location of at least one cell structure within a cell and/or other similar biological or nonbiological structure, a method of displaying a location of at least one subject particle, method for increasing the dynamic range of a 3D image acquired from samples containing weak and strong sources of light, and method for sharpening a 3D image in a vertical direction.

Loading CytoViva collaborators
Loading CytoViva collaborators