Grenoble, France
Grenoble, France

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Patent
Cytoo Inc and Consejo Superior De Investigationes Cientifcas | Date: 2012-07-24

The present invention relates to devices and associated methods for forming three-dimensional multicellular assemblies in vitro. Specifically, the present invention relates to devices comprising at least one three-dimensional multicellular assembly immobilised on a two-dimensional adhesive pattern, wherein said three-dimensional multicellular assembly has an organised structure with a normalised polarity and methods for the formation of three-dimensional multicellular assemblies having an organised structure.


PubMed | Cytoo Inc
Type: | Journal: Journal of visualized experiments : JoVE | Year: 2010

To date, most HCA (High Content Analysis) studies are carried out with adherent cell lines grown on a homogenous substrate in tissue-culture treated micro-plates. Under these conditions, cells spread and divide in all directions resulting in an inherent variability in cell shape, morphology and behavior. The high cell-to-cell variance of the overall population impedes the success of HCA, especially for drug development. The ability of micropatterns to normalize the shape and internal polarity of every individual cell provides a tremendous opportunity for solving this critical bottleneck (1-2). To facilitate access and use of the micropatterning technology, CYTOO has developed a range of ready to use micropatterns, available in coverslip and microwell formats. In this video article, we provide detailed protocols of all the procedures from cell seeding on CYTOOchip micropatterns, drug treatment, fixation and staining to automated acquisition, automated image processing and final data analysis. With this example, we illustrate how micropatterns can facilitate cell-based assays. Alterations of the cell cytoskeleton are difficult to quantify in cells cultured on homogenous substrates, but culturing cells on micropatterns results in a reproducible organization of the actin meshwork due to systematic positioning of the cell adhesion contacts in every cell. Such normalization of the intracellular architecture allows quantification of even small effects on the actin cytoskeleton as demonstrated in these set of protocols using blebbistatin, an inhibitor of the actin-myosin interaction.


Cytoo Inc | Entity website

Head Quarter Minatec - BHT - Bt. 52 7 parvis Louis Nel 38040 Grenoble cedex 9 Phone : +33 (0)4 38 88 47 05


2D+

Trademark
Cytoo Inc | Date: 2012-09-19

Cell culture vessels, in coverslip, slide or microplate formats arrayed with extracellular matrix proteins, antibodies, peptides, receptors, receptor domains or fluorescent derivatives of the above, for cell culture and analysis; laboratory consumable kits comprised primarily of cell culture vessels and reagents for pharmaceutical, cosmetical and skin care or scientific research use; laboratory equipment and instruments, namely, cell cultures vessels for cell analysis, high content analysis and cell screening, for the life sciences research market and the pharmaceutical industry market. Training and teaching services in cell analysis, high content analysis and cell screening, employing the technologies of cell adhesive micropatterns; arranging and conducting colloquiums, conferences and seminars in cell analysis, high content analysis and cell screening using the technologies of cell adhesive micropatterns. Cell based assay development services for others for pharmaceutical, cosmetical and skin care and scientific research purposes, namely, custom development of cell based assays and high content cell screening (HCS) services; development of new products for others in the fields of tools for pharmaceutical research, cosmetical and skin care, and cell biology research; development of new computer software for others to collect, analyze and organize data from cell images; providing an online computer database featuring scientific research on cell morphology and biomarker localization on cells; computer services, namely, providing temporary use of online non-downloadable software for accessing and analyzing information in cell images.


Trademark
Cytoo Inc | Date: 2011-07-19

Laboratory equipment and instruments, namely, cell culture vessels for cell analysis, high content analysis and cell screening, for the life sciences research market and the pharmaceutical industry market; computer software for cell and molecular analysis, high content analysis and cell screening, other than for medical use.


The invention relates to a device for standardizing myoblast differentiation into myotubes, comprising a substrate (1) and at least one cell-adhesive pattern (2) for culturing myoblasts on said substrate, wherein: The invention is also a method for standardizing myoblast differentiation into myotubes, comprising: Another aspect of the invention is a method for compounds screening in cell-based assays related to myotoxicity and drug discovery.


Degot S.,Cytoo Inc
Journal of visualized experiments : JoVE | Year: 2010

To date, most HCA (High Content Analysis) studies are carried out with adherent cell lines grown on a homogenous substrate in tissue-culture treated micro-plates. Under these conditions, cells spread and divide in all directions resulting in an inherent variability in cell shape, morphology and behavior. The high cell-to-cell variance of the overall population impedes the success of HCA, especially for drug development. The ability of micropatterns to normalize the shape and internal polarity of every individual cell provides a tremendous opportunity for solving this critical bottleneck (1-2). To facilitate access and use of the micropatterning technology, CYTOO has developed a range of ready to use micropatterns, available in coverslip and microwell formats. In this video article, we provide detailed protocols of all the procedures from cell seeding on CYTOOchip micropatterns, drug treatment, fixation and staining to automated acquisition, automated image processing and final data analysis. With this example, we illustrate how micropatterns can facilitate cell-based assays. Alterations of the cell cytoskeleton are difficult to quantify in cells cultured on homogenous substrates, but culturing cells on micropatterns results in a reproducible organization of the actin meshwork due to systematic positioning of the cell adhesion contacts in every cell. Such normalization of the intracellular architecture allows quantification of even small effects on the actin cytoskeleton as demonstrated in these set of protocols using blebbistatin, an inhibitor of the actin-myosin interaction.


Cytoo Inc | Entity website

The CYTOO assay development advantage Physiologically-relevant cellular models Micropatterning controls cell microenvironment: it enables to choose from several adhesion molecules and attachment areas and geometries, cell densities, and promotes cell-cell junctions. In turn, the physical and biochemical cues received by cells change their genomic transcription and thus their phenotype ...


Cytoo Inc | Entity website

Head Quarter Minatec - BHT - Bt. 52 7 parvis Louis Nel 38040 Grenoble cedex 9 Phone : +33 (0)4 38 88 47 05


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