Kumari R.,Amity University |
Singh M.,CytoGene Research and Development |
Singh S.,Amity University |
Joshi T.,Vydehi Institute of Biotech science |
And 2 more authors.
International Journal of Pharma and Bio Sciences | Year: 2013
There are several water-borne diseases which are highly pathogenic for humans. Amongst all, genus Vibrio plays a major role. In this case study of "RAPID IDENTIFICATION OF HUMAN PATHOGENIC VIBRIO SPECIES USING MULTIPLEX PCR" We have chosen salt water bodies such as sea and estuaries for sampling. To facilitate the identification of human-pathogenic species, we have used multiplex PCR to amplify gene regions in five species (V. cholerae, V. parahaemolyticus, V. vulnificus, V. mimicus and V. alginolyticus). The assay was tested on a sample of 82 Vibrio isolates for identification of five common human pathogenic vibrio species. Multiplex PCR was performed targeting the tox gene present in the five most common pathogenic vibrio species. After analysis of all the results we have found that salt water is having a higher concentration of vibrio species than freshwater & V.vulnificus was most abundant among all.
Sharma H.K.R.,Cytogene Research and Development |
Bajpai K.,Institute of Applied Medicine and Research |
Srivastava R.,Cytogene Research and Development |
Singh S.K.,Cytogene Research and Development
International Journal of Pharma and Bio Sciences | Year: 2014
Microfungi produce mycotoxins that are secondary metabolites and are capable of causing death and disease in human beings, plants and animal too. The pharmacological effects of mycotoxins and mycotoxin derivatives are helpful in use as antibiotics, promoters, and other kinds of drugs; still others have been implicated as chemicalwarfare agents. This work focuses on the most important ones including aflatoxins and fumonisins and their effect on medicinal plants widely found in Indian sub-continent viz., Withania somnifera and Ocimum sanctum. Fungal degraded fruits, Pyrus sp., Malus domestica and Musa sp. were taken for extraction of mycotoxin by high-speed blending with Acetone and Chloroform, followed by purification with Column Chromatography (Magnesium Silicate) of the crude extract. TLC was performed to determine the Rf value of purified toxins. Aflatoxin B1, Aflatoxin B2 andFumonisin B1 were identified by this technique and further detection of mycotoxin was done by Gas Chromatography. Purified mycotoxins were then sprayed in equal amount (̃5ml) on stem, leaves and roots of the potted plants (Ashwagandha and Tulsi) and the results were quite impressive. Wilting and necrosis occurred on the leaf portion and only necrosis occurred on the stem portion of the plant. Withania sp. showed great results as compared to Ocimum sp. with respect to susceptibility against mycotoxin. Capitulum inflorescence of Ocimum sp. fell offafter aweek of spraying. Spraying was done with an interval of 2 days alternatively on both the plants with similar dosage of purified toxins. It was done till 16 days.
Ali D.,King Saud University |
Verma A.,Indian Institute of Toxicology Research |
Pathak A.K.,Cytogene Research and Development |
Ali H.,Maulana Azad National Institute of Technology |
Hans R.K.,Indian Institute of Toxicology Research
Chemistry and Ecology | Year: 2016
The depletion of the ozone layer and enhancement of solar radiation may exert an adverse influence on the ecosystem. Phototoxicity of sludge and polycyclic aromatic hydrocarbons (PAHs) under ultraviolet radiation (UVR) to seedling was studied. Seeds of wheat (Triticum aestivum) were planted in sludge and PAHs (anthracene (An), benzo(a)pyrene (BaP) and pyrene (Py)) with and without UVR. Toxicity of sludge increased in the presence of UVR in wheat. UVB radiation was found to be more hazardous than UVA radiation. Results demonstrated that An, BaP and Py induced phototoxicity at various concentrations (1–10 µg/mL) under UVA (1.5 mW/cm2) or UVB (1.08 J/cm2) exposure. The pattern of phototoxicity was An > Py > BaP to shoot length, root length, and fresh weight; chlorophyll, protein content, enzyme activity of catalase and α-amylase were reduced while the activity of superoxide dismutase and starch was enhanced. Reduction in seedling growth and biochemical parameters may be related to less photosynthesis, less nutritional uptake, and distortion of root cap. Thus, the synergistic effect may be due to alterations in photosynthesis, phytohormones, or nutritional uptake. © 2016 Taylor & Francis.