Botsaris G.,University of Cyprus |
Nikolaou K.,University of Cyprus |
Liapi M.,Cyprus Veterinary Services Nicosia Cyprus |
Pipis C.,Cyprus Veterinary Services Nicosia Cyprus
Journal of Food Safety | Year: 2016
Listeria monocytogenes is an important foodborne pathogen which causes listeriosis in humans and animals. Presence of Listeria spp. and L. monocytogenes in raw bovine milk could lead to the manufacture of contaminated products due to lack or insufficient pasteurization or cross-contamination. The purpose of this study was to record the prevalence of Listeria spp. and L. monocytogenes in all the Cypriot dairy farms, using bulk tank milk (BTM) samples. A total of 205 raw BTM samples were analyzed for Listeria spp. and L. monocytogenes. In addition a total viable count (TVC) was also performed in parallel in an attempt to correlate high TVC with the presence of Listeria. Results revealed a total of three samples (1.46%; 95% CI: 0.3-4.4%) positive for Listeria spp. two (0.98%; 95% CI: 0.04-3.72%), of which were successfully identified as L. monocytogenes. Molecular serotyping indicated that the two isolates were both 1/2a. This is the first report in Cyprus documenting the prevalence of Listeria spp. and L. monocytogenes in the raw BTM of all Cypriot dairy farms revealing a low prevalence in a reported asymptomatic population. Monitoring the prevalence is essential in an attempt to protect animal health and welfare as well as human health by reducing the risk of listeriosis through consumption of milk and dairy products. © 2016 Wiley Periodicals, Inc.