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Guwāhāti, India
Guwāhāti, India
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Hussain I.,Assam Agricultural University | Sharma R.K.,Assam Agricultural University | Borah P.,Assam Agricultural University | Rajkhowa S.,National Research Center on Pig | And 8 more authors.
Anaerobe | Year: 2015

One hundred and seventeen faecal samples from pet dogs (pup = 21 and adult = 96) brought for treatment to a veterinary clinic were examined for Clostridium difficile. A total of 16 (13.67%) samples were positive. Nine (56.25%) isolates were obtained from 17 adult dogs undergoing antibiotic treatment and this was significantly higher (p < 0.01) as compared to isolates from dogs without antibiotic treatment. Ten isolates (62.5%) were toxigenic (all toxinotype 0) and six were non-toxigenic. None of the isolates were positive for binary toxin genes. PCR ribotyping revealed three different ribotypes (012, 014 and 046) among A+B+ isolates and five different ribotypes (010, SLO 131, and ACD 001 to ACD 003) among A-B- isolates. The PFGE analysis of toxigenic isolates revealed three different pulsotypes corresponding to the PCR ribotypes. © 2015 Elsevier Ltd.


PubMed | Assam Agricultural University, Indian National Institute of Cholera and Enteric Diseases, University of Maribor, CVSc and 3 more.
Type: Journal Article | Journal: Molecular and cellular probes | Year: 2016

A total of 1034 samples were collected from different sources and C.difficile was isolated from 18 (9.04%) of 199 human, 9 (4.89%) of 184 cattle, 29 (12.44%) of 233 pig, and from 23 (13.94%) of 165 poultry samples. Variations were observed on the rate of isolation according to age and clinical conditions (diarrhoea). None of the samples from cow, sheep, goat, local chicken, and wild animals yielded any C.difficile. Out of those isolates, 8, 2, 19 and 6 isolates from human, cattle, pig and poultry, respectively were toxigenic. The toxigenic isolates carried both tcdA, and tcdB (A


PubMed | University of Maribor, Assam Agricultural University, National Research Center on Pig, State Biotech Hub and CVSc
Type: | Journal: Anaerobe | Year: 2015

One hundred and seventeen faecal samples from pet dogs (pup = 21 and adult = 96) brought for treatment to a veterinary clinic were examined for Clostridium difficile. A total of 16 (13.67%) samples were positive. Nine (56.25%) isolates were obtained from 17 adult dogs undergoing antibiotic treatment and this was significantly higher (p < 0.01) as compared to isolates from dogs without antibiotic treatment. Ten isolates (62.5%) were toxigenic (all toxinotype 0) and six were non-toxigenic. None of the isolates were positive for binary toxin genes. PCR ribotyping revealed three different ribotypes (012, 014 and 046) among A(+)B(+) isolates and five different ribotypes (010, SLO 131, and ACD 001 to ACD 003) among A(-)B(-) isolates. The PFGE analysis of toxigenic isolates revealed three different pulsotypes corresponding to the PCR ribotypes.


Reddy B.S.,Teaching Veterinary Clinical Complex Veterinary Medicine | Sivajothi S.,C.V.Sc. | Rayulu V.C.,C.V.Sc.
Comparative Clinical Pathology | Year: 2015

A 5-year-old dog was identified suffering with recurrent skin lesions, fever, and progressive weight loss. Peripheral blood smear revealed presence of Babesia organisms. Dog had anemia, leucopenia, thrombocytopenia and it was treated with diminazene aceturate along with supportive therapy. © 2015, Springer-Verlag London.


PubMed | Teaching Veterinary Clinical Complex Veterinary Medicine and C.V.Sc.
Type: Journal Article | Journal: Journal of parasitic diseases : official organ of the Indian Society for Parasitology | Year: 2016

Inone year period of study, dogs with inappetence, fever, ocular discharges, dullness, enlarged lymph nodes were screened for the presence of haemoprotozoans at College Hospital of College of Veterinary Science, Tirupati. Wet blood film examination and stained blood smear examination was done to confirm the condition. Peripheral blood smears of dogs revealed the presence of Trypansomes. Trypanosoma evansi was confirmed based on the morphology and measurements of the organisms in the stained blood smears. Haematology revealed decreased total erythrocyte count, packed cell volume, haemoglobin and total leucocyte count values. Total serum proteins, albumin and glucose levels were decreased significantly (P<0.01), but a significant increase (P<0.01) in cholesterol, blood urea nitrogen, serum alkaline phosphatase, serum aspartate aminotransferase and serum alanine aminotransferase levels were observed. Decreased total T4 and free T4 were also observed in the T. evansi infected dogs.

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