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Guwāhāti, India

Subhashini N.,VAS | Krishnaiah N.,CVSc | Bindu Kiranmayi C.,CVSc
International Journal of Pharma and Bio Sciences

A total of 105 shell fish samples (35 each of prawn, shrimp and crab) collected from local markets were subjected to cultural methods and PCR for the presence of V. parahaemolyticus and their toxins using primers for toxR, tdh and trh genes respectively. Out of total 105 samples, positive isolates of V. parahaemolyticus were found in 17 prawn samples, 25 shrimp and 19 crab samples by cultural method where as by PCR assay the organism was found in 24 samples of prawn, 33 shrimp and 27 crab samples. Out of 84 positive isolates of V. parahaemolyticus by PCR method, 11 showed presence of tdh, 37 had trh and 6 had both tdh and trh. Selective broths (Alkaline PeptoneWater and Salt Polymyxin Broth) were more efficient than non-selective broths (Brain Heart Infusion and Tryptic Soy Broth) and Salt Polymyxin Broth (SPB) was superior over Alkaline Peptone Water (APW) in selective broths for isolation of this organism by both cultural and PCR methods. The sensitivity of PCR method for this organism was 2.5cfu. Source

Hussain I.,Assam Agricultural University | Sharma R.K.,Assam Agricultural University | Borah P.,Assam Agricultural University | Rajkhowa S.,National Research Center on Pig | And 8 more authors.

One hundred and seventeen faecal samples from pet dogs (pup = 21 and adult = 96) brought for treatment to a veterinary clinic were examined for Clostridium difficile. A total of 16 (13.67%) samples were positive. Nine (56.25%) isolates were obtained from 17 adult dogs undergoing antibiotic treatment and this was significantly higher (p < 0.01) as compared to isolates from dogs without antibiotic treatment. Ten isolates (62.5%) were toxigenic (all toxinotype 0) and six were non-toxigenic. None of the isolates were positive for binary toxin genes. PCR ribotyping revealed three different ribotypes (012, 014 and 046) among A+B+ isolates and five different ribotypes (010, SLO 131, and ACD 001 to ACD 003) among A-B- isolates. The PFGE analysis of toxigenic isolates revealed three different pulsotypes corresponding to the PCR ribotypes. © 2015 Elsevier Ltd. Source

Bindu Kiranmayi C.,CVSc | Krishnaiah N.,CVSc | Subhashini N.,VAS | Maheswari M.,CVSc
International Journal of Pharma and Bio Sciences

Apart from cattle, sheep and goat are important natural reservoirs of Shigatoxigenic Escherichia coli. Faecal contamination during dressing of carcass accounts for the presence of STEC in raw meat. A total of 201 samples (104 sheep faecal and 97 sheep farm water samples) collected from various sources were subjected to PCR analysis for the presence of Shigatoxigenic Escherichia coli using primers specific to STEC virulent genes, shiga toxins (stx1 and stx2) and enterohaemolysin (hlyA) with amplification size of 614bp, 779bp and 361bp respectively. Out of 201 samples, 93 showed presence of STEC (36 faecal and 27 water samples). Of the 93 STEC positive isolates, 63 (67.74%) showed presence of stx1, 32 (34.4%) showed stx2, 47 (50.53%) showed hlyA gene and 16 (17.2%) showed both stx1 and stx2. Among 58 STEC positive isolates, 7.52% (4 faecal and 3 water samples) isolates possessed all the 3 virulent genes. Enrichment with modified Tryptic Soy Broth (mTSB) containing novobiocin gave good results compared to modified Escherichia coli (mEC) broth with novobiocin. Source

Reddy B.S.,Teaching Veterinary Clinical Complex Veterinary Medicine | Sivajothi S.,CVSc | Rayulu V.C.,CVSc
Comparative Clinical Pathology

A 5-year-old dog was identified suffering with recurrent skin lesions, fever, and progressive weight loss. Peripheral blood smear revealed presence of Babesia organisms. Dog had anemia, leucopenia, thrombocytopenia and it was treated with diminazene aceturate along with supportive therapy. © 2015, Springer-Verlag London. Source

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