Chandler, AZ, United States
Chandler, AZ, United States

Time filter

Source Type

Czogalla A.,TU Dresden | Kauert D.J.,University of Munster | Franquelim H.G.,Max Planck Institute of Biochemistry | Uzunova V.,CUBE Technology | And 3 more authors.
Angewandte Chemie - International Edition | Year: 2015

We report a synthetic biology-inspired approach for the engineering of amphipathic DNA origami structures as membrane-scaffolding tools. The structures have a flat membrane-binding interface decorated with cholesterol-derived anchors. Sticky oligonucleotide overhangs on their side facets enable lateral interactions leading to the formation of ordered arrays on the membrane. Such a tight and regular arrangement makes our DNA origami capable of deforming free-standing lipid membranes, mimicking the biological activity of coat-forming proteins, for example, from the I-/F-BAR family. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


PubMed | TU Dresden, CUBE Technology, Max Planck Institute of Biochemistry and University of Munster
Type: Journal Article | Journal: Angewandte Chemie (International ed. in English) | Year: 2015

We report a synthetic biology-inspired approach for the engineering of amphipathic DNA origami structures as membrane-scaffolding tools. The structures have a flat membrane-binding interface decorated with cholesterol-derived anchors. Sticky oligonucleotide overhangs on their side facets enable lateral interactions leading to the formation of ordered arrays on the membrane. Such a tight and regular arrangement makes our DNA origami capable of deforming free-standing lipid membranes, mimicking the biological activity of coat-forming proteins, for example, from the I-/F-BAR family.


Sheppard V.C.,Georgia Institute of Technology | Scheffel A.,Georgia Institute of Technology | Scheffel A.,Max Planck Institute of Molecular Plant Physiology | Poulsen N.,Georgia Institute of Technology | And 2 more authors.
Applied and Environmental Microbiology | Year: 2012

Living organisms are adept in forming inorganic materials (biominerals) with unique structures and properties that exceed the capabilities of engineered materials. Biomimetic materials syntheses are being developed that aim at replicating the advantageous properties of biominerals in vitro and endow them with additional functionalities. Recently, proof-of-concept was provided for an alternative approach that allows for the production of biomineral-based functional materials in vivo. In this approach, the cellular machinery for the biosynthesis of nano-/micropatterned SiO2 (silica) structures in diatoms was genetically engineered to incorporate a monomeric, cofactor-independent ("simple") enzyme, HabB, into diatom silica. In the present work, it is demonstrated that this approach is also applicable for enzymes with "complex" activity requirements, including oligomerization, metal ions, organic redox cofactors, and posttranslational modifications. Functional expression of the enzymes β-glucuronidase, glucose oxidase, galactose oxidase, and horseradish peroxidase in the diatom Thalassiosira pseudonana was accomplished, and 66 to 78% of the expressed enzymes were stably incorporated into the biosilica. The in vivo incorporated enzymes represent approximately 0.1% (wt/wt) of the diatom biosilica and are stabilized against denaturation and proteolytic degradation. Furthermore, it is demonstrated that the gene construct for in vivo immobilization of glucose oxidase can be utilized as the first negative selection marker for diatom genetic engineering. © 2012, American Society for Microbiology.


Grant
Agency: Department of Defense | Branch: Air Force | Program: SBIR | Phase: Phase I | Award Amount: 100.00K | Year: 2010

This program is expected to result in a high-temperature heat pipe passive cooling system for space-borne phased array antennas. The innovative approach chosen for this project will result in a reliable g-force independent cooling system that is robust against launch and vibration forces as well as the zero-g environment of space. The system is lightweight, inexpensive, and efficient, and has the potential to significantly reduce the payload weight of satellites by enabling high-temperature components to dissipate effectively through higher temperature radiators. BENEFIT: The cooling system resulting from this effort is expected to be significantly smaller and lighter-weight than current ammonia heat pipes used for thermal management in satellite systems. This device will directly address the needs of the space market but will be applicable to several other markets including airborne flight hardware. This system has potential commercial application both military and commercial aircraft and can be applied to high-end servers for more effective chip cooling. Beyond passive cooling, this technology can be modified to become a microclimate refrigeration system with application to Firefighters, police, and HAZMAT teams, as well as military personnel.


Grant
Agency: Department of Defense | Branch: Air Force | Program: SBIR | Phase: Phase I | Award Amount: 100.00K | Year: 2010

This program is expected to result in a compact, high energy density, silent propulsion system for small unmanned autonomous systems (SUAS). The innovative approach chosen for this project will substantially increase both range and time aloft of miniature unmanned air vehicles (UAVs), while using far less electricity. This will leave more available electricity for sensors and communications resulting in more eyes on more targets for longer times. BENEFIT: This propulsion system is a significant advancement in the area of small to micro-UAVs. It substantially reduces the weight of UAV propulsion system while remaining quiet and enabling much longer missions and higher battery payload for increased UAV effectiveness. The system will be lightweight, inexpensive, and efficient, enabling it to replace conventional electric propeller systems on numerous platforms. It can address the needs of many market segments beyond the military, including Homeland Security, agriculture, and geologic mapping.


A method and an apparatus for achieving transformation of a virtual view into a 3D view are provided. The method comprises: S1. capturing position coordinates of a human eye by a human-eye tracking module; S2. determining a rotation angle of a virtual scene and rotating the virtual scene according to the rotation angle to obtain a virtual holographic 3D view matrix by a first image processing module; S3. determining a shearing angle for each of viewpoints to generate a shearing matrix for the viewpoint in one-to-one correspondence, and post-multiplying the shearing matrix with a corresponding viewpoint model matrix to generate a left view and a right view by a second image processing module; and S4. projecting the left view and the right view of each of the viewpoints by the projection displaying module.


A display apparatus and a visual displaying method for simulating a holographic 3D scene are provided. The display apparatus comprises: a display screen; two front-facing 3D cameras, being configured to capture human-eye 3D position information; a 3D human-eye tracking algorithm processing unit, being configured to generate a first signal and a second signal according to the human-eye 3D position information; a 3D scene generating unit, being configured to receive the first signal, render and acquire the corresponding left visual-channel 3D scene and the corresponding right visual-channel 3D scene according to the first signal; and an electronic grating, being configured to receive the second signal and adjust the working angle according to the second signal so that the left visual-channel 3D scene is incident into the left eye of the user and the right visual-channel 3D scene is incident into the right eye of the user.


Grant
Agency: Department of Defense | Branch: Defense Advanced Research Projects Agency | Program: SBIR | Phase: Phase II | Award Amount: 750.00K | Year: 2011

This program is expected to result in a combined, miniature Power/Microclimate cooling system for the soldier. The compact thermoelectric generator system will create significant power and have higher energy density than lithium technology batteries over extended missions. The highly integrated, and innovative microclimate cooler will result in a gravity-independent cooling system with high coefficient of performance and minimum weight. The combined system re-uses or integrates virtually every element of either an independent power generation system or microclimate cooling system. The result is a very compact dual use device capable of being worn by the soldier.


Grant
Agency: Department of Defense | Branch: Navy | Program: SBIR | Phase: Phase I | Award Amount: 100.00K | Year: 2010

This program is expected to result in a miniature drying and microclimate cooling system for the interior of MOPP Level 4 protective suits. The innovative approach chosen for this project will result in a gravity-independent drying/microclimate cooling system that operates with JP-5, or other heavy fuels, universally available to the user, enabling continuous use over extended missions. The integrated power system will create all of the electrical power necessary for the system, eliminating the need for batteries to run the system. The result will be a very compact climate control system that will keep users of MOPP Level 4 and commercial HAZMAT personal protective suits both cool and dry.


Grant
Agency: Department of Defense | Branch: Navy | Program: SBIR | Phase: Phase II | Award Amount: 1.00M | Year: 2012

This program is to develop a compact, portable refrigeration and heating system for the Marines that interfaces with existing gear, including a MOPP suit worn under full battle dress. This system will effectively cool the user in the most demanding high-temperature environments while alternately acting as a heating system for both cold-weather operations and to prevent hypothermia (a particularly life-threatening condition for wounded individuals). The system safely operates on refined fuel-oils including JP-8 and Diesel to drive the refrigeration system. Fuel is a very compact energy source, and as such, only small amounts of it will be needed (approximately 1 oz./hour) for extended missions. Particular attention will be paid to user safety in this system.

Loading CUBE Technology collaborators
Loading CUBE Technology collaborators