CSU Analytical Spectrometry

Saint Petersburg, Russia

CSU Analytical Spectrometry

Saint Petersburg, Russia
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Yaroshenko I.S.,CSU Analytical Spectrometry | Khaimenov A.Ya.,Dr Reddys Laboratories In Russian Federation | Grigoriev A.V.,Saint Petersburg State Polytechnic University | Sidorova A.A.,Saint Petersburg State Polytechnic University
Journal of Analytical Chemistry | Year: 2014

A method is proposed for determining rhein (an active metabolite of diacerein) in human blood plasma by reversed-phase high-performance liquid chromatography with UV detection. The analyte was selectively extracted from blood plasma by liquid-liquid extraction with a 5% solution of tert-butyl methyl ether in ethyl acetate. The recovery of rhein is 68 ± 3%, LOQ 100 ng/mL. The stability of the analytes and linearity, selectivity, sensitivity, reproducibility, and accuracy of the developed method are evaluated. The pharmacokinetics of diacerein in the oral administration of drug formulations by 18 healthy volunteers is studied. © 2014 Pleiades Publishing, Ltd.


Alekseeva A.V.,CSU Analytical Spectrometry | Kartsova L.A.,Saint Petersburg State University
Journal of Analytical Chemistry | Year: 2011

The analytical potencies of ligand-exchange capillary electrophoresis (LECE) in regard to the determination of bioactive substances are discussed. As it is shown, complexation with copper(II) in the electrophoretic determination with UV detection makes possible the determination of amino acids, amines, and sugars, which do not absorb in the UV region, with the limits of detection 5-10 mg/L, and reduce the detection limits of the absorbing analytes, such as tryptophane, tyrosine, and histamine by 2-3 times. Prospects for using online preconcentration for the reduction of c min are shown on an example of aliphatic amino acids, i.e., thirtyfold preconcentration is attained. LECE is shown to be applicable to the simultaneous determination of glucose, Na( +), and K(+) in human blood serum. Also versions of ligand-exchange capillary electrophoresis with direct UV detection and capillary zone electrophoresis with indirect one are compared in terms of efficiency, time of analysis, and limit of detection. The potencies of the LECE method are compared with those of ligand-exchange chromatography. © 2011 Pleiades Publishing, Ltd.


Sidorova A.A.,CSU Analytical Spectrometry | Kartsova L.A.,Saint Petersburg State University | Grigoriev A.V.,CSU Analytical Spectrometry | Protoschak V.V.,Kirov Military Medical Academy | Murashko E.A.,Saint Petersburg State University
Journal of Analytical Chemistry | Year: 2010

A procedure was developed for the determination of an anticancer drug of mitomycin C (MMC) in urinary bladder tissue samples by reversed-phase high-performance liquid chromatography with spectrophotometric (365 nm) and mass-spectrometric detection with detection limits of 50 and 10 ng/mL, respectively (signal-to-noise ratio = 3). The tissue samples were homogenized and solid-phase extraction was performed on a DSC-18 cartridge. The procedure was used for comparing the effectiveness of the MMC penetration into the urinary bladder tissue by passive diffusion and by intravesical drug electrophoresis. EDMA increases the rate of mitomycin penetration into urinary bladder walls by 3 to 5 times with respect to passive diffusion. © Pleiades Publishing, Ltd., 2010.


Kartsova L.A.,Saint Petersburg State University | Alekseeva A.V.,CSU Analytical Spectrometry
Journal of Analytical Chemistry | Year: 2011

The fundamentals of ligand-exchange capillary electrophoresis are discussed, and the potential of the method in solving major problems, such as the separation of enantiomers and the determination of biologically active compounds poorly absorbing in the UV region (sugars, amines, and amino acids) is considered. © 2011 Pleiades Publishing, Ltd.

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