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Pālampur, India

Rani S.,Maharaja Lakshman Sen Memorial College MLSM | Kumari S.,Punjabi University | Gupta R.C.,Punjabi University | Chahota R.K.,CSK HPKV
Plant Systematics and Evolution | Year: 2014

During the present study, 360 populations of 174 species belonging to 82 genera and 23 families of dicots have been cytologically worked out from different localities of District Kangra (Himachal Pradesh) between 550 and 3,800 m altitude. As many as 10 species including Berberis ceratophylla (2n = 28), Caltha alba (2n = 32), Corydalis meifolia (2n = 16), C. thyrsiflora (2n = 16), Impatiens reidii (2n = 14), Indigofera hamiltonii (2n = 16), Potentilla thomsonii (2n = 14), Sedum trifidum (2n = 36), Stellaria semivestita (2n = 26) and Viola canescens (2n = 12) have been cytologically worked out for the first time at world level. New intraspecific diploid cytotypes have been recorded in 11 species while new intraspecific tetraploid cytotypes are noticed in 15 species. B-chromosomes have also been reported in 3 species viz. Anemone obtusiloba, Clematis grata and Ranunculus diffusus for the first time at world level. The species of the area are active state of evolution, depicting heterogeneity in chromosome numbers involving polyploidy (48 species, 28.18 %) as well as irregular meiotic behavior. The meiotic abnormalities have been witnessed in 69 species (39.65 %) showing different types of irregularities such as cytomixis, interbivalant connections, unoriented bivalents, chromatin stickiness, chromosomal laggards and bridges as well as abnormal microsprogenesis. All these meiotic abnormalities lead to reduced pollen fertility and formation of variable sized pollen grains in most of these species. © 2013 Springer-Verlag Wien.


Dubey Y.P.,CSK HPKV
African Journal of Microbiology Research | Year: 2011

Soil reaction (pH) is important property soil for crop productions. Many chemicals as ameliorates are required to make soil productive. In organic agriculture, bio-ameliorates are prerequisite. Rhizobia are acid producers and Bradyrhizobia are alkali producers. Rhizobia and Bradyrhizobia are used as ameliorate to study their impact on soil reaction (pH) in laboratory conditions for 21 days. It was observed that Rhizobia decreased soil pH and Bradyrhizobia increased soil pH during incubation. © 2011 Academic Journals.


Kashyap L.,Lovely Professional University | Sharma D.C.,CSK HPKV
Environmental Monitoring and Assessment | Year: 2015

An established analytical method was further standardized for the estimation of spinosad residue in tomato grown under naturally ventilated polyhouse situated in mid-hills of Himachal Pradesh, India. Spinosad was sprayed on hybrid tomato cv. Avtar at the recommended and double the recommended dosages at 15 and 30 g a.i. ha−1, respectively, to control the lepidopterous pests and the residue were analysed from tomato fruits at different intervals after second spray. The samples were extracted with acetonitrile-water mixture (8:2), partitioned into dichloromethane, methanol and anhydrous sodium hydroxide. The extracts were concentrated under vacuum and cleaned up with silica solid phase extraction cartridge. Further, the samples were analysed in high-performance liquid chromatography equipped with UV/visible detector at λ = 250 nm and C18 reversed-phase column. Consistent recoveries ranging from 80.6 ± 1.1 to 91.4 ± 1.2 % were observed when samples were spiked at fortification range of 0.01 to 0.20 mg kg−1. The limit of quantification of the method was worked out to be 0.02 mg kg−1. The half-life values of spinosad were determined to be 1.20 and 1.60 days at recommended and double the recommended dosage, respectively. The safety interval for spinosad sprayed tomato fruit was determined to be 1.92 and 3.88 days at application rate of 15 and 30 g a.i. ha−1, respectively. © 2015, Springer International Publishing Switzerland.


Vignesh M.,Indian Agricultural Research Institute | Hossain F.,Indian Agricultural Research Institute | Nepolean T.,Indian Agricultural Research Institute | Saha S.,Indian Agricultural Research Institute | And 4 more authors.
Indian Journal of Genetics and Plant Breeding | Year: 2012

Carotenoids are the major sources of dietary precursor of vitamin A and act as potential antioxidant besides preventing diseases such as night blindness in humans. Vitamin A deficiency is a global problem, but is particularly prevalent in developing countries like India, where 31 percent of pre-school children are reportedly affected. Evaluation of genetic variability for kernel β-carotene in 105 maize inbreds of diverse pedigree from India-and CIMMYT-origin revealed significant variation ranging from 0.02 to 16.50 ìg/g. One of the key reasons for wide variation of kernel β-carotene is due to the allelic variation at crtRB1 3'TE gene. Five among 95 inbreds possessed the favourable crtRB1 3'TE allele with a mean β-carotene concentration of 0.86 ìg/g. In contrast, the same allele detected in 20 CIMMYT-Maize HarvestPlus genotypes contributed a mean kernel β-carotene concentration of 11.29 ìg/g. The contrast in β-carotene concentration in Indian and CIMMYT genotypes could be attributed to the presence of SNPs and InDels in crtRB1 3'TE locus, along with the presence/absence of favourable alleles of other important genes influencing the carotenoid biosynthetic pathway. Marker-assisted breeding has been initiated, to introgress the crtRB1 3'TE favourable allele using high β-carotene CIMMYT inbreds as donors, to develop provitamin A-rich maize cultivars suitable for maize growing regions in India.


Chandel R.S.,CSK HPKV | Banyal D.K.,CSK HPKV | Singh B.P.,Central Potato Research Institute Campus | Malik K.,Central Potato Research Institute Campus | Lakra B.S.,CCS Haryana Agricultural University
Potato Research | Year: 2010

This paper reviews the importance of Potato apical leaf curl virus (PALCV) in India, the biology of its major vector Bemisia tabaci and possible control measures of PALCV and B. tabaci. PALCV has appeared as a new problem in potato growing areas of northwestern plains of India. The first report of PALCV was made from Hissar around 2000. PALCV is a whitefly (B. tabaci) transmitted viral disease and the symptoms are manifested primarily in the form of upward or downward curling of leaves, with 40-75% infection being recorded in cultivars grown in the Indo-Gangetic Plains of India. B. tabaci is a polyphagous pest that feeds on numerous fibre (particularly cotton), food, vegetable and ornamental plants. B. tabaci produces many generations in a year and reaches high population densities. To check the disease incidence, whitefly suppression using imidacloprid either as seed treatment or as foliar application early in the season is recommended. To minimize within-field spread of viruses, removal of virus-infected plants is suggested. Application of mineral and vegetable oils has been found to inhibit virus transmission and possibly can help to avoid difficulties with insecticide resistance in whiteflies. © 2010 EAPR.

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