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Sharma K.,CSK Himachal Pradesh Krishi Vishvavidyalya | Singh N.,CSK Himachal Pradesh Krishi Vishvavidyalya
Indian Journal of Agricultural Biochemistry | Year: 2011

French bean (Phaseolus vulgaris L.) is a popular green vegetable grown throughout the country for its delicious pods. In recent years, cultivation of French bean for green pods has been found to be profitable enterprise in Himachal Pradesh due to favourable moderate temperature during flowering and pod formation stage resulting in quality pods along with higher yield. The present research work was planned with special reference to assess the nutritional attributes of promising genotypes at different picking stages. Significant variations in moisture, crude protein, total ash, ascorbic acid, total carotenoids, sodium and potassium, total phenols and anti-nutritional factor tannin were observed at three different stages of picking. Fifteen genotypes of French bean were subjected to biochemical estimation and different genotypes emerged to be superior at different picking stages. Genotypes VLB-9, VLFB-2003 followed by MFB-2 and KPV-2, were found to be superior as compared to other genotypes, in order of excellence based on cumulative rating for all the traits at first, second and third stages of picking. Source

Thakur N.,CSK Himachal Pradesh Krishi Vishvavidyalya | Singh N.,CSK Himachal Pradesh Krishi Vishvavidyalya
Indian Journal of Agricultural Biochemistry | Year: 2011

Fifteen pea genotypes/varieties were analyzed to evaluate biochemical constituents and to identify the extent of diversity by molecular characterization. The range of variation for biochemical constituents varied significantly viz. moisture(67.52-81.06 per cent), crude protein(18.00-24.46 per cent), crude fat (1.23-3.55 per cent), crude fibre (7.86-13.45 per cent), carbohydrate (55.21-63.17 per cent), ash (1.95-3.63 per cent), sodium (35.01-99.80 mg/100g), potassium (234.98-385.52 mg/ 100g), ascorbic acid (3.01-6.35mg/100g), total carotenoids (9.37-14.20 mg/100g) and total chlorophyll (2.30-4.2mg/100g) content. The cumulative varietal grading showed that genotypes DPP-168, 08/PMVAR/1 and VL-8 occupied first, second and third rank in order of excellence. SDS-PAGE and RAPD analysis were also carried out for genetic diversity. Seed protein profiling by SDS-PAGE analysis of collected genotypes revealed a total of 10 protein bands. Dendrogram based on electrophoretic data using UPGMA divided the genotypes in 2 major clusters. The overall similarity coefficient ranged from 0.11 to 1.00. For DNA fingerprinting by RAPD, a total of 40 primers were tried to obtain reproducible bands, out of which only 12 primers resulted into reproducible bands. A total of 60 highly reproducible bands were obtained out of which 56 were polymorphic. The polymorphism ranged from 87.5 to 100 per cent. Dendrogram generated using UPGMA separated 15 genotypes into 2 major clusters with 25 to 82 per cent similarity. The above results revealed high genetic diversity in some of selected genotypes. Therefore, the selected genotypes possessed variability both in genetic and biochemical attributes which can be exploited for further improvement of this crop. Source

Nagal K.B.,CSK Himachal Pradesh Krishi Vishvavidyalya | Gupta A.,CSK Himachal Pradesh Krishi Vishvavidyalya | Asrani R.K.,CSK Himachal Pradesh Krishi Vishvavidyalya
Indian Journal of Animal Sciences | Year: 2014

An outbreak of nitrate poisoning due to accidental ingestion of Salvia coccinea vernacularly called Kali phool by a flock of 300 migratory Gaddi goats with 20% mortality was reported during winter in Kangra Valley, Himachal Pradesh, India. The history, clinical signs, characteristic post mortem findings, presence of excess level of nitrates in the foliage of Salvia coccinea plants (12.63% on dry matter basis) and 6.2 mg % of nitrite contents in the sera of ailing goats evidences the nitrate poisoning owing to Salvia coccinea (Kali phool) among Gaddi goats. Source

Awasthi C.P.,CSK Himachal Pradesh Krishi Vishvavidyalya | Kumar A.,CSK Himachal Pradesh Krishi Vishvavidyalya | Singh N.,CSK Himachal Pradesh Krishi Vishvavidyalya | Thakur R.,CSK Himachal Pradesh Krishi Vishvavidyalya
Indian Journal of Agricultural Biochemistry | Year: 2011

Thirteen genotypes of amaranth procured from NBPGR Research Station, Shimla during the year 2009 were evaluated for various physical and biochemical constituents of dietary significance by following standard procedures. Significant variation in biochemical constituents viz. moisture (8.76 - 9.98 %), crude protein (9.65 - 12.69 %), oil (3.24 - 6.06 %), ash (2.02 - 2.91 %), crude fibre (2.85 - 4.77%), carbohydrate (64.40 - 71.92 %), total sugars (3.08-3.29 %), methionine (1.26 - 1.94 % protein), tryptophan (0.75 - 1.03 % protein), in vitro protein digestibility (66.09 - 76.74 %), total phenols (123 - 153 mg/100g), oxalate (85 -129 mg/100g), calcium (147 - 201 mg/100g), phosphorus (449 - 526 mg/100g), iron (4.60 -6.55 mg/100g) and zinc (3.08 - 4.72 mg/ 100g) among various amaranth genotypes was observed. Cumulative genotypic ranking done for desirable quality traits taken together indicated genotype(s) IC-423117, IC-422845 and IC-422652 as versatile genotypes in order of excellence. Source

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