Sanberg P.R.,University of South Florida |
Eve D.J.,University of South Florida |
Willing A.E.,University of South Florida |
Garbuzova-Davis S.,University of South Florida |
And 5 more authors.
Stem cell transplantation is a potentially important means of treatment for a number of disorders. Two different stem cell populations of interest are mononuclear umbilical cord blood cells and menstrual blood-derived stem cells. These cells are relatively easy to obtain, appear to be pluripotent, and are immunologically immature. These cells, particularly umbilical cord blood cells, have been studied as either single or multiple injections in a number of animal models of neurodegenerative disorders with some degree of success, including stroke, Alzheimer's disease, amyotrophic lateral sclerosis, and Sanfilippo syndrome type B. Evidence of anti-inflammatory effects and secretion of specific cytokines and growth factors that promote cell survival, rather than cell replacement, have been detected in both transplanted cells. Copyright © 2011 Cognizant Comm. Corp. All rights reserved. Source
Borlongan C.V.,University of South Florida |
Kaneko Y.,University of South Florida |
Maki M.,Georgia Regents University |
Yu S.-J.,University of South Florida |
And 5 more authors.
Stem Cells and Development
Cell therapy remains an experimental treatment for neurological disorders. A major obstacle in pursuing the clinical application of this therapy is finding the optimal cell type that will allow benefit to a large patient population with minimal complications. A cell type that is a complete match of the transplant recipient appears as an optimal scenario. Here, we report that menstrual blood may be an important source of autologous stem cells. Immunocytochemical assays of cultured menstrual blood reveal that they express embryonic-like stem cell phenotypic markers (Oct4, SSEA, Nanog), and when grown in appropriate conditioned media, express neuronal phenotypic markers (Nestin, MAP2). In order to test the therapeutic potential of these cells, we used the in vitro stroke model of oxygen glucose deprivation (OGD) and found that OGD-exposed primary rat neurons that were co-cultured with menstrual blood-derived stem cells or exposed to the media collected from cultured menstrual blood exhibited significantly reduced cell death. Trophic factors, such as VEGF, BDNF, and NT-3, were up-regulated in the media of OGD-exposed cultured menstrual blood-derived stem cells. Transplantation of menstrual blood-derived stem cells, either intracerebrally or intravenously and without immunosuppression, after experimentally induced ischemic stroke in adult rats also significantly reduced behavioral and histological impairments compared to vehicle-infused rats. Menstrual blood-derived cells exemplify a source of "individually tailored" donor cells that completely match the transplant recipient, at least in women. The present neurostructural and behavioral benefits afforded by transplanted menstrual blood-derived cells support their use as a stem cell source for cell therapy in stroke. © Mary Ann Liebert, Inc. 2010. Source
Cryo Cell International Inc. | Date: 2009-10-26
Skin grafts; mesh skin grafts; human autograft and allograft tissue; engineered skin graft substitutes; cultured epithelial skin grafts; mesh skin grafts, allograft tissue, and autograft tissue; skin grafts and mesh skin grafts of cultured epithelial cells and biodegradable scaffolds; human allograft tissue; and human autograft tissue.
Cryo Cell International Inc. | Date: 2011-09-20
All purpose carrying bags, carry-all bags, bags for carrying babies accessories, beach bags, shoulder bags, tote bags, travel bags, and backpacks.
Cryo Cell International Inc. | Date: 2008-12-18
Body fluid collection kit used for collecting body fluids, cells, and tissue, or menstrual blood, cells, fluid and tissue, comprised primarily of medical specimen containers for fluid, cell, and tissue collection, and also comprised of tubes, test tubes, and hermetically sealable containers containing a buffer solution. medical services, namely, infectious disease screening, genetic disease screening, blood screening, drug screening, and DNA screening.