Jeje S.O.,Cross River University of Technology
Nigerian journal of physiological sciences : official publication of the Physiological Society of Nigeria | Year: 2015
It has been reported in human and animal studies that early exposure to glucocorticoids could retard growth and subsequent development of cardio metabolic diseases. Chronic exposure to glucocorticoids induced oxidative stress. Therefore, the role of oxidative stress in some of the observed metabolic imbalance needs to be elucidated. This study examined the effects of lactational dexamethasone exposure on metabolic imbalance and oxidative stress marker in the liver of male offspring of exposed mother. Twenty lactating dams were divided into 4 groups of 5 animals each. Group 1 was administered 0.02 ml/100gbwt/day normal saline through lactation days 1-21. Group 2, 3, and 4 were administered 100 µg/kgbwt/day dexamethasone for lactation days 1-7, 1-14, and 1-21 respectively. The male offspring were thereafter separated and sacrificed at 12weeks of age for evaluation of lipid profile and oxidative stress marker in the liver. Results from this study indicate that Total Cholesterol (TC), Triglycerides (TAG) and LDL- cholesterol (LDL-C) were significantly higher in the Dex 1-7, Dex 1-14 and Dex 1-21 groups when compared with the control. HDL-Cholesterol (HDL-C) was significantly reduced in the Dex 1-7, Dex 1-14 and Dex 1-21 groups relative to the control. Basal Fasting Blood Sugar (FBS) was also significantly higher in the Dex 1-14 and Dex 1-21 groups when compared with the control. Liver malondialdehyde was significantly higher in the Dex1-14 and Dex1-21 group compared to the control. However, liver catalase and SOD activity were all significantly lower in Dex 1-7, Dex 1-14 and Dex 1-21 groups relative to control. Liver protein was significantly lower in the Dex1-14 and Dex1-21 treatment groups when compared with the control. Findings from this study suggest that there is possible increase in metabolic imbalance in the offspring of mother exposed to dexamethasone during lactation and these effects may be secondary to increase oxidative stress in the liver.
Ijomone O.M.,Obafemi Awolowo University |
Ijomone O.M.,Cross River University of Technology |
Obi A.U.,Obafemi Awolowo University |
Obi A.U.,Imo State University
Annals of Neurosciences | Year: 2013
Background: Kolaviron, isolated from seeds of Garcinia kola, have been shown to possess wide pharmacological properties. Purpose: The present study examined the effect of kolaviron on acetylcholinesterase activities in the hippocampus and striatum of adult Wistar rats. Methods: In this study, histological and histochemical methods were used to investigate the effects of kolaviron on the histology of the hippocampus and striatum and on acetylcholinesterase activities in these brain regions. Results: We showed that kolaviron produced no neurodegenerative changes in the hippocampus and striatum. Kolaviron did not significantly alter (p<0.05) neuronal density in these brain regions. Kolaviron significantly reduced (p<0.05) acetylcholinesterase staining intensity, suggesting a likely inhibiting effect on this enzyme. Conclusion: To the best of our knowledge, this study provides the first evidence that kolaviron could act as an acetylcholinesterase inhibitor. Kolaviron may be developed as a herbal-based natural product with therapeutic potential in the management of neurodegenera-tive disorders associated with disturbed cholinergic neurotransmitter systems.
Uttah E.,Cross River University of Technology |
Ibeh D.C.,Landmarks Hospital
Journal of Vector Borne Diseases | Year: 2011
Background & objectives: The study was aimed at determining the pattern of co-occurrence of species of microfilaraemia between onchocerciasis endemic and sporadic populations. Methods: From every consenting person of one year and above, 50 μl of day and night blood samples were collected and processed respectively with Haemotoxylin and Giemsa as vital stains. Two skin snips (one each from the waist and the shoulder) were also taken from these individuals and processed. Results: Results showed single species microfilaraemia (86.4 and 82.3%), double species microfilaraemia (12.2 and 16.9%) and triple species microfilaraemia (1.4 and 0.7%) for endemic and sporadic populations respectively. All the species had single species microfilaraemia mostly, but Mansonella perstans and Loa loa showed greatest tendency towards double and triple species microfilaraemia. The prevalence of Wuchereria bancrofti microfilaraemia among those positive for Onchocerca volvulus was significantly lower than the overall prevalence of Wuchereria bancrofti. Wuchereria bancrofti microfilaraemia was most common among those who had L. loa microfilaraemia. Wuchereria bancrofti microfilarial intensity was higher among those with M. perstans microfilaraemia than among those positive for any of the other filarial species. Similarly, the intensity of M. perstans microfilaraemia among those positive for W. bancrofti exceeded the overall intensity of M. perstans. Conclusion: It is concluded that there was no definite pattern in mf densities discernible from co-occurrence infections either in the onchocerciasis endemic or sporadic population. There could be varied outcomes of onchocerciasis infection attributable to positive or negative regulatory effects of other pathogens harbored by the victims.
Letta B.D.,Ambo University |
Attah L.E.,Cross River University of Technology
Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes | Year: 2013
Residue levels of organochlorine pesticides (OCP) in a total of 90 cattle samples comprising meat, liver and kidney collected from carcasses slaughtered in six towns in West Shoa Zone, Ethiopia, (Ambo, Guder, Ginchi, Gedo, Holeta and Tikur Inchini), have been determined. The pesticides were extracted by solid phase extraction (SPE) and quantification was carried out using gas chromatography-mass spectrometry (GC-MS). A good linearity (r2 > 0.998) was found in the range 0.001-7.00 mg/kg for the samples studied. Most of the pesticides had recoveries in the range 81-99% and values of relative standard deviation (RSD) <7.2% for repeatability and reproducibility, showing good accuracy and precision of the method. The concentration level of the studied organochlorines followed the order: p, p' dichloro-diphenyl-trichloroethane (DDT) > endosulfan>o,p′-DDT >lindane>dieldrin>endrin>aldrin>chlorothanolin while the order of contamination in the analyzed organs was liver > kidney > meat. Heat treatment of the meat, kidney and liver samples (boiling for 90 min.) produced an overall reduction of 62.2%, 44.5%, 37.7%, 29%, 31%, 34.3% and 30.8% in lindane, o, p′-DDT, endosulfan, p, p′-DDT, chlorothanolin, aldrin, dieldrin, and endrin, respectively. Although the residual contents of the organochlorines detected in all the contaminated samples analyzed from the six cities were below the respective maximal permissible levels set by international organizations, samples from Holeta town were more contaminated and may necessitate effective monitoring as bioaccumulation of these residues may pose health problems in human beings. © 2013 Copyright Taylor and Francis Group, LLC.
Ephraim P.E.,Girne American University |
Ephraim P.E.,Cross River University of Technology
Ethics and Information Technology | Year: 2013
With rising numbers of Facebook, Twitter and MXit users, Africa is increasingly gaining prominence in the sphere of social networking. Social media is increasingly becoming main stream; serving as important tools for facilitating interpersonal communication, business and educational activities. Qualitative analyses of relevant secondary data show that children and youths aged between 13 and 30 constitute Africa's heaviest users of social media. Media reports have revealed cases of abuse on social media by youths. Social networks have severally been used as tools for perpetuating crimes such as; cyberbullying and violence against girls and women. This study proposes a 'Culture-centered Approach' to the use of social media in a bid to minimize these cybercrimes and encourage the responsible use of social media amongst African youths. The Culture-centered Approach, which incorporates the tenets of Information Ethics, stresses the need for the respect of the dignity and rights of other online users as well the application of good cultural values and ethical behavior while on social media platforms. © 2013 Springer Science+Business Media Dordrecht.
Abara A.E.,Cross River University of Technology
Pakistan Journal of Nutrition | Year: 2011
The proximate principles and mineral elements in the wet and dry tissue and peel of Dioscorea bulbifera were determined using standard methods. The proximate principles were analyzed using AOAC methods, the mineral elements namely calcium, magnesium, iron, zinc, copper, sodium and potassium by absorption and emission spectrophotometry and phosphorous by molybdovanadate method. All the parameters analyzed apart from carbohydrate and energy value showed tissue/peel ratios of less than 1.0 which was consistent with the observed significantly lower levels of the mineral elements and proximate principles in the tissue than the peel (p<0.01) excepting carbohydrate and energy value. Additionally, the about constant tissue/peel ratios observed for the mineral elements and some proximate principles such as ash, crude fibre, fat and protein for the wet and dry samples of Dioscorea bulbifera were indicative of the stability of these nutrients and in about the same proportions in the tissue and peel upon drying at 40°C. The nutritional significance of the mineral elements in the tissue and peel of Dioscorea bulbifera was also highlighted in the discussion. © Asian Network for Scientific Information, 2011.
Ikpi G.,Cross River University of Technology |
Offem B.,Cross River University of Technology
Revista de Biologia Tropical | Year: 2011
Bacterial infection among the most common cultured mudfish Clarias gariepinus in Africa, has become a cause of concern, because it constitutes the largest economic loss in fish farms. In order to provide useful biological data of the pathogens for good management practices, samples were collected monthly between January 2008 and December 2009 in three monoculture nursery ponds, located in three different positions: upriver (A, grassland), mid-river (B, mixed forest and grassland) and downriver (C, rainforest) along 200km length of Cross River floodplains, Nigeria. A total of 720 fingerlings between 15.1 and 20.7g were analyzed to determine the degree of infection. The bacterial pathogens were taken from their external surfaces, and were isolated and identified by standard methods. The caudal fins of fingerlings from pond A had the highest bacterial load (5.8x103cfu/g), while the least counts (1.2×103cfu/g) were identified on the head of fish from pond C, with Flexibacter columnaris as the major etiological agent. Pseudomonas fluorescens, Aeromonas hydrophila, Escherichia coli, Staphylococcus aureus and Micrococcus luteus were identified as co-isolates with P. fluorescens as dominant (0.7x102cfu/mL) co-isolates in pond water. Clinical signs of five white spots with red periphery appeared on the external surface of infected fish. All the fish sampled, died after 4 to 9 days. There was no significant difference in the bacterial counts between different ponds, but the difference between fish organs/parts examined was significant. Fish from these ponds are therefore potentially dangerous to consumers and highly devalued, with the economic impact to producers. Preventive methods to avoid these infections are recommended.
Ugot M.,Cross River University of Technology
International Journal of Applied Linguistics and English Literature | Year: 2014
This paper focuses on informal language usage by students of Cross River University of Technology, Calabar, Nigeria. This includes the use of slang words from primarily the substrate and superstrate language of the mother tongue (MT) and Nigerian Pidgin (NP) respectively. Participant observation was used for this work including lectures and other forums. Focus is on the speech discourse of students. The NP in particular is a contact language which has grown in stature due to factors of relevance as a common language in a pluralistic society like the Cross River State in particular, and Nigeria in general. Its use has also expanded due to urbanization. The MT has been observed to be influential through direct translation of certain expressions. Findings have shown that the informal use of language by the students has inadvertently affected their competence in standard and formal language use. © Australian International Academic Centre.
Uttah E.C.,Cross River University of Technology
Iranian Journal of Parasitology | Year: 2010
Background: Onchocerciasis is endemic in the Imo River Basin, Nigeria. This study was aimed at assessing the prevalence and intensity of microfilaria of Onchocerca volvulus in the area. Methods: A cross-sectional study was carried out in the Okigwe Local Government Area, Imo State, Nigeria. Two skin snips (one from the waist and another from the shoulder) were taken from 1024 individuals examined. The survey coverage was high (91.8% of the study population). An individual was considered mf positive if either of the waist or shoulder snips or both were mf positive. The SPSS for Windows package was used for entering and analysis of data. Results: Thirty-seven percentage of those examined was positive for Onchocerca volvulus microfilariae (39.2% of males and 34.9% of females). The mf prevalence increased steadily with increasing age to reach 70.4% in the oldest age group. The overall mf Geometric Mean Intensity among mf positive individuals was 16 mf/skin snip and was significantly higher among males (18 mf/skin snip) than females (14 mf/skin snip) (p < 0.01). A scatter plot of microfilariae numbers in snips from the waist against numbers in snips from the shoulder of the same individuals, showed close correlation (Pearson's correlation coefficient = +0.90; p < 0.01), and those with mf intensities below 10 mf/snip had a more scattering tendency away from the regression line than those with higher mf intensities. Conclusion: Onchocerciasis is a public health concern in the area. Perhaps, 10 mf/snip is critical intensity threshold for reliable sampling using corneo-scleral punch.
Uttah E.C.,Cross River University of Technology
Journal of Vector Borne Diseases | Year: 2011
Background & objectives: The study was aimed at determining the prevalence and intensity of Wuchereria bancrofti microfilaraemia in a high altitude region of south-eastern Nigeria, and ascertaining the prevalence of clinical signs and symptoms associated with the filarial infections. Methods: Thick smear of 50 μl finger-prick blood collected at night between 2200 and 0200 hrs from consenting persons were stained with Giemsa and examined microscopically in a cross-sectional study. Consenting individuals were examined for various gradations of hydrocele, limb and scrotal elephantiasis by qualified medical personnel. Results: The prevalence of W. bancrofti microfilaraemia was 4.3%, highest in the older people but comparable in both sexes. The overall microfilarial (mf) geometric mean intensity (GMI) among mf positive individuals was 123 mf/ml of blood (138 mf/ml for males and 110 mf/ml of blood for females); and rose significantly with increasing age (one-way analysis of variance; p < 0.001). Prevalence of clinical manifestations was: hydrocele (7.1%), scrotal elephantiasis (4%), and limb elephantiasis (6%). The mf GMI was significantly higher among those without hydrocele or limb elephantiasis than among those with the clinical manifestations (t-test; p <0.05 for both tests); the opposite was the case for scrotal elephantiasis, (t-test; p <0.01). Conclusion: Filariasis is endemic in the high altitude region of south-eastern Nigeria. The chronic clinical manifestations observed there underscore the need for urgent combination therapy interventions.