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Tuyet N.T.,Vietnam Academy of Agricultural science VAAS | Waeyenberge L.,Belgium Institute for Agricultural and Fisheries Research | Elsen A.,Bodemkundige Dienst van Belgie | Nhi H.H.,Food Crops Research Institute | And 2 more authors.
Russian Journal of Nematology | Year: 2014

Random amplified polymorphic DNA (RAPD) analysis of the complete genome and sequencing of the D2-D3 expansion segments of the 28S rDNA géhe were carried out to compare the intraspecific genomic variability of ten Prarylenchus coffeae populations collected from different agricultural crops in different agro-ecological regions in Vietnam. In addition, the RAPD bands were compared with these of P. speUeri, a species recently described from Ghana, while the D2-D3 sequences were compared with these of P. coffeae and closely related Prazylenchus species available in the GenBank database. As determined by RAPD bands analysis of the complete genome, genomic similarity did not correspond either with geographic or original host plant origin of the Vietnamese P. coffeae populations. As determined by sequence analysis of the D2-D3 28S rDNA expansion fragments, all ten P. coffeae populations from Vietnam examined were closely related to each other and with the P. coffeae populations of which the D2-D3 28S rDNA expansion fragments sequences were available from GenBank. Both the RAPD bands analysis of the complete genome and the sequence analysis of the D2- D3 28S rDNA expansion fragments indicate genetic divergence among the ten P. coffeae populations from Vietnam examined on the one hand and P. spe(jeri from Ghana on the other hand, confirming the validity of the latter species.

Nguyen T.D.,James Cook University | Nguyen T.D.,Food Crops Research Institute | Lawn R.J.,James Cook University | Bielig L.M.,James Cook University
Crop and Pasture Science | Year: 2012

The expression and inheritance of several qualitative traits was examined in four cultivatedwild hybrid populations involving each of two mungbean (Vigna radiata ssp. radiata) cultivars, cvv. Berken and Kiloga, and each of two Australian accessions of the wild subspecies (V. radiata ssp. sublobata). One of the wild accessions, ACC 1, was representative of a prostrate, fine-stemmed, gracile type and the other, ACC 87, was representative of a more robust perennial form endemic in north-eastern Australia. For each of the four cultivatedwild populations, trait expression was observed in plants from the parent, F1, F2, and the two F1parental backcross generations, when grown under favourable conditions in large pots on benches in the field at CSIRO Davies Laboratory, Townsville, Australia. Models of inheritance were inferred based on the segregation patterns in the different generations of the cultivated v. wild phenotypes. For most traits, the model of inheritance depended more on the wild than the cultivated parent, with more traits in the crosses involving ACC 1 being digenic than in those involving ACC 87. For all the observed morphological and seed traits, the wild phenotype was dominant, consistent with the cultivated phenotype having arisen through mutations that inhibited expression of the wild type. In contrast, the apparent resistance of the wild parents to field strains of powdery mildew disease was recessive to the strong susceptibility of the two cultivars. The segregation patterns for presence or absence of tuberous roots were remarkably similar in the two crosses involving the perennial accession ACC 87, and were consistent with the formation of tuberous roots being conditioned by two complementary, dominant genes. The fact that an apparently complex trait like perenniality might be conditioned by so few genes suggested that perenniality may also be an ancestral wild trait, disruption of which has led to the now more common, annual form. Linkage analyses suggested that perenniality was associated with the wild-type seed traits, black speckled testa and pigmented hilum, which previous molecular studies have indicated are both located on mungbean linkage group 2. © 2012 CSIRO.

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