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Sundernagar, India

Prabhakar C.S.,CSK Himachal Pradesh Krishi Vishvavidyalaya | Prabhakar C.S.,Research Complex for Eastern Region Research Center | Mehta P.K.,CSK Himachal Pradesh Krishi Vishvavidyalaya | Sood P.,CSK Himachal Pradesh Krishi Vishvavidyalaya | And 5 more authors.
Genetica | Year: 2012

Population genetic structure of melon fly analysed with mitochondrial cytochrome oxidase I gene suggested that melon fly populations across the globe is homogeneous with non-significant variation of 0. 000-0. 003 base substitutions per site. Test isolates representing various geographic situations across the world were placed in 26 mitochondrial haplotypes based on variations associated with a maximum of three mutational steps and the predominant haplotype i. e. H1 was present in all melon fly populations except Hawaiian population. Evolution of mtCOI gene suggested that the fly could have originated some 0. 4 million years ago. The present study also indicated that the B. cucurbitae population expansion is an event of post Pleistocene warm climatic conditions with small number of founder population. The invasion of B. cucurbitae in Hawaii was associated with the large population size and the global presence of the fly is associated with human mediated dispersal. The very low genetic variation suggested that the fly management might be possible by large scale sterile insect techniques programme. © 2012 Springer Science+Business Media B.V. Source


Prabhakar C.S.,CSK Himachal Pradesh Krishi Vishvavidyalaya | Prabhakar C.S.,Indian Council of Agricultural Research | Sood P.,CSK Himachal Pradesh Krishi Vishvavidyalaya | Sood P.,Crop Research Sub Station | Mehta P.K.,CSK Himachal Pradesh Krishi Vishvavidyalaya
Archives of Phytopathology and Plant Protection | Year: 2012

During the course of studies, Bactrocera (Bactrocera) latifrons (Hendel), B. (B.) nigrofemoralis White and Tsuruta, Dacus (Callantra) longicornis Wiedemann, Dacus (Callantra) sphaeroidalis (Bezzi), Cyrtostola limbata (Hendel) and Pliomelaena udhampurensis Agarwal and Kapoor were recorded for the first time in Himachal Pradesh in a cucurbit ecosystem. Apart from these, other species viz. Bactrocera tau, Bactrocera cucurbitae, Bactrocera dorsalis, Bactrocera zonata, Bactrocera scutellaris, Bactrocera diversa and Dioxyna sororcula (Wiedemann) were also identified. Distribution records of B. (B.) dorsalis (Hendel), B. (B.) zonata (Saunders), Bactrocera (Hemigymnodacus) diversa (Coquillett), B. (Zeugodacus) cucurbitae (Coquillett), B. (Z.) scutellaris (Bezzi) and B. (Z.) tau (Walker) has been described. © 2012 Copyright Taylor and Francis Group, LLC. Source


Prabhakar C.S.,CSK Himachal Pradesh Krishi Vishvavidyalaya | Prabhakar C.S.,Indian International Crops Research Institute for the Semi Arid Tropics | Sood P.,CSK Himachal Pradesh Krishi Vishvavidyalaya | Sood P.,Crop Research Sub Station | And 4 more authors.
Phytoparasitica | Year: 2013

Gut bacteria of fruit fly, Bactrocera tau (Walker) (Diptera: Tephritidae), were isolated and the isolates attractive to B. tau adults were characterized using morphological, biochemical and 16S rRNA analyses to determine their taxonomic position. Based upon morphological, biochemical and 16S rRNA sequences (on the basis of closest match), five gut bacterial species of B. tau were characterized as Delftia acidovorans, Pseudomonas putida, Flavobacterium sp., Defluvibacter sp. and Ochrobactrum sp., of which four bacterial isolates, viz., Delftia acidovorans, Flavobacterium sp., Defluvibacter sp. and Ochrobactrum sp. are new records from guts of the fruit fly species. © 2012 Springer Science+Business Media Dordrecht. Source


Prabhakar C.S.,CSK Himachal Pradesh Krishi Vishvavidyalaya | Prabhakar C.S.,Bhabha Atomic Research Center | Sood P.,CSK Himachal Pradesh Krishi Vishvavidyalaya | Sood P.,Crop Research Sub Station | And 2 more authors.
Biochemical Systematics and Ecology | Year: 2013

The population genetic structure of the pumpkin fruit fly, Bactrocera tau, a fruit fly pest that causes significant losses to cucurbit cultivations, has been studied in Himachal Pradesh (India) using mitochondrial cytochrome oxidase I (mtCOI) gene sequences. Levels of differentiation (genetic distances and FST values) among samples from different locations are minimal, suggesting the local occurrence of a large and geographically undifferentiated population, with the possible exception of population Solan. Nevertheless, overall genetic variability is substantial, with 10 different haplotypes detected in 16 individuals and only one of these - likely the original one as it occupies a central position in the network and is found at a relatively high frequency -shared between multiple populations. The phylogenetic analysis of local B. tau samples in the context of the different sibling species that constitute the B. tau complex in its South-East Asia region of origin revealed that local B. tau is closely related to B. tau species A from Thailand. This should be taken into account in any intervention aimed at the control of this pest, e.g. area wide integrated pest management (AW IPM). The marked local genetic uniformity and predominance of one single species of the species complex further suggest that the sterile insect technique (SIT) may be a viable option. © 2013 Elsevier Ltd. Source

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