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Yang Y.,Nanjing Agricultural University | Zheng G.,Nanjing Agricultural University | Han L.,Nanjing Normal University | Dagang W.,Nanjing Agricultural University | And 6 more authors.
Theoretical and Applied Genetics | Year: 2013

Soybean mosaic virus (SMV) is one of the most broadly distributed soybean (Glycine max (L.) Merr.) diseases and causes severe yield loss and seed quality deficiency. Multiple studies have proved that a single dominant gene can confer resistance to several SMV strains. Plant introduction (PI) 96983 has been reported to contain SMV resistance genes (e.g., Rsv1 and Rsc14) on chromosome 13. The objective of this study was to delineate the genetics of resistance to SMV in PI 96983 and determine whether one gene can control resistance to more than one Chinese SMV strain. In this study, PI 96983 was identified as resistant and Nannong 1138-2 was identified as susceptible to four SMV strains SC3, SC6, SC7, and SC17. Genetic maps based on 783 F2 individuals from the cross of PI 96983 × Nannong 1138-2 showed that the gene(s) conferring resistance to SC3, SC6, and SC17 were between SSR markers BARCSOYSSR_13_1114 and BARCSOYSSR_13_1136, whereas SC7 was between markers BARCSOYSSR_13_1140 and BARCSOYSSR_13_1185. The physical map based on 58 recombinant lines confirmed these results. The resistance gene for SC7 was positioned between BARCSOYSSR_13_1140 and BARCSOYSSR_13_1155, while the resistance gene(s) for SC3, SC6, and SC17 were between BARCSOYSSR_13_1128 and BARCSOYSSR_13_1136. We concluded that, there were two dominant resistance genes flanking Rsv1 or one of them at the reported genomic location of Rsv1. One of them (designated as "Rsc-pm") conditions resistance for SC3, SC6, and SC17 and another (designated as "Rsc-ps") confers resistance for SC7. The two tightly linked genes identified in this study would be helpful to cloning of resistance genes and breeding of multiple resistances soybean cultivars to SMV through marker-assisted selection (MAS). © Springer-Verlag Berlin Heidelberg 2013.

Wang D.,Nanjing Agricultural University | Wang D.,Crop Institute of Anhui Academy of Agricultural science | Ma Y.,Nanjing Agricultural University | Liu N.,Nanjing Agricultural University | And 3 more authors.
Plant Breeding | Year: 2011

Soybean mosaic virus (SMV) disease is one of the most destructive viral diseases for soybeans worldwide. In this study, 1047F2 plants derived from 'Dabaima' (resistant)×'Nannong1138-2' (susceptible) were used to study inheritance and resistance gene linkage to SMV strain SC4. SSR were used to establish genetic maps of the parent plants, in addition to resistant or susceptible F2 generation plants. Results indicated that a single dominant gene (designated RSC4) located on chromosome 14 (MLG B2) controls resistance to SC4 infections. In addition, SNP and genomic-SSR markers near RSC4 were examined. Based on the linkage analysis of the population, the genomic-SSR markers BARCSOYSSR-14-1413 and BARCSOYSSR-14-1416 were found flanking RSC4. Sequence analysis of the soybean genome indicated that the interval between the two genomic-SSR markers was <100kb on chromosome 14. Quantitative real-time PCR (QRT-PCR) analysis of the 100-kb region identified three genes (Glyma14g38510, 38560 and 38580) likely involved in regulating resistance to SMV. These results are useful for transferring and pyramiding or map-based cloning of RSC4. © 2011 Blackwell Verlag GmbH.

Wu Q.,Nanjing Agricultural University | Wu Q.,Crop Institute of Anhui Academy of Agricultural science | Wang H.,Nanjing Agricultural University | Wu J.,Nantong University | And 5 more authors.
Journal of Plant Biology | Year: 2015

The evaluation and use of endogenous soybean genes is an effective strategy to minimize the yield losses caused by insects. Allene oxide cyclase (AOC) catalyzes the most important step in the biosynthesis of jasmonate (JA), which plays a crucial role in plant defense against insects. In this study, the role of GmAOC3 in plant insect resistance was evaluated. Real-time PCR results indicate that GmAOC3 was uniquely and rapidly activated and attained peak expression in leaves after attack by the common cutworm (CCW). In insect bioassays, transgenic lines overexpressing GmAOC3 were significantly less damaged than wild-type plants, and the relative growth rate of CCW fed with leaves from transgenic lines was significantly lower than that of CCW fed with leaves from wild-type plants. Electron microscopy revealed that the density of leaf trichomes in transgenic lines overexpressing GmAOC3 was greater than that in wild-type tobacco. Several physiological and morphological indicators, including JA, phenolic content and the relative expression levels of the putrescine N-methyltransferase (PMT) and proteinase inhibitor (PI) genes, phenylalanine ammonia lyase (PAL) activity and volatile substances, increased in the transgenic plants overexpressing GmAOC3. Our findings indicate that GmAOC3 plays an important role in soybean resistance to CCW and can be used as a resource for plant breeding. © 2015, Korean Society of Plant Biologists and Springer-Verlag Berlin Heidelberg.

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