Moorthy K.K.,Acharya N.G. Ranga Agricultural University |
Babu P.,Acharya N.G. Ranga Agricultural University |
Sreedhar M.,Quality Control Laboratory |
Sama V.S.A.K.,Crop Improvement Section |
And 3 more authors.
Seed Science and Technology | Year: 2011
The present investigation was carried out with the following objectives: (i) to identify EST-SSR marker allele(s) capable of distinguishing 12 popular rice varieties cultivated in India, and (ii) to validate the utility of the genotype specific EST-SSR markers in seed genetic purity assessments. A set of 98 EST-SSR markers located across the 12 chromosomes of rice were used for finger-printing the selected rice varieties, out of which 24 EST-SSR markers were observed to be informative enough to distinguish all the 12 rice varieties considered in the study. A set of nine EST-SSR markers (RMES2-1, RMES2-2, RMES7-2, RMES12-2, JGT03-29.2, JGT07-22.8, JGT07-25.2, JGT10-5.7 and JGT12-18.6) exhibited unique alleles for seven rice varieties (Swarna, MTU 1001, Tellahamsa, Krishnahamsa, JGL 384, JGL 1798 and Vikas) and could serve as molecular IDs (Identities) for these varieties. To validate the utility of the EST-SSR markers in genetic purity assessment, foundation seed lots of MTU 1001, Tellahamsa, Krishnahamsa and Vikas were assessed for their genetic purity using both EST-SSR marker analysis and 12 morphological characters in a grow-out test (GOT). The impurities detected in the EST-SSR marker analysis were 2-3% higher as compared to those detected based on morphological characters in GOT. The results indicated practical utility of the EST-SSR markers in assessing the genetic purity of the rice varieties.
Revathi P.,Crop Improvement Section |
Medoju P.,Crop Improvement Section |
Singh A.K.,Crop Improvement Section |
Sundaram R.M.,Crop Improvement Section |
And 8 more authors.
Indian Journal of Genetics and Plant Breeding | Year: 2013
To assess the efficiency of different molecular markers which are linked to fertility restorer genes Rf3 and Rf4 of WA-CMS system, 103 breeding lines with no information on fertility restoration were screened with the help of molecular markers linked to major fertility genes Rf3 and Rf4. The breeding lines were crossed with APMS6A and the F1s were evaluated for pollen and spikelet fertility to identify restorers and maintainers. The SSR primer RM6100 linked to Rf4 gene of chromosome 10 and RM10313 linked to Rf3 gene of chromosome 1 showed eighty five and eight one percentage efficiency respectively in identifying restorer lines. Therefore, these markers are useful tool for evaluating large number of breeding lines to know about their fertility restoration in a short period of time without generating and evaluating large number of test crosses.The potential restorers may be identified with hundred percentage efficiency based on molecular screening itself, if candidate genes based markers are developed and validated for both Rf4 and Rf3 genes.
Rani N.S.,Crop Improvement Section |
Sivaranjani A.K.P.,Crop Improvement Section |
Madhav M.S.,Crop Improvement Section |
Sundaram R.M.,Crop Improvement Section |
And 5 more authors.
Indian Journal of Genetics and Plant Breeding | Year: 2011
The process of determining major cooking and taste qualities are biochemical and, unraveling the quality is possible only after grain maturity which is time consuming and laborious. Thus, there is a need for identification of molecular markers linked to major quality traits which can help rice breeders in selecting progeny with desirable quality traits at early stages of development.Till date, most of the work done related to identification of markers linked to grain quality traits has been done in the japonlca germplasm. Hence the present study was carried out with the objectives of (i) validation of markers reported to be linked to grain quality traits in indica germplasm, (ii) identification of more efficient functional markers for the cloned genes associated with grain quality, and (iii) identification of major QTL's controlling cooking and eating quality traits in rice. Association study carried out with 13 reported markers in a set of 360 indica genotypes showed varied levels of association for each trait. Most of these markers were not useful in indica germplam. A functional marker named BADEX7-5 targeting the 8-bp deletion in the exon-7 of BAD-2 (candidate gene for aroma), which can clearly differentiate the aromatic and non-aromatic genotypes efficiently on a simple agarose gel has been identified in this study. In addition, a functional marker named DRR GL-1 targeting an SNP in the major QTL GS3, which is associated with kernel length (KL) and kernel elongation (KE) was identified and validated through QTL-marker linkage analysis involving large populations. Major QTLs for gelatinization temperature, grain breadth, grain length, length to breadth ratio and kernel elongation were identified.The identification of candidate genes in the QTL s may give some vital clues in understanding the genetic control of key grain quality traits of indica genotypes and provide additional markers for immediate use in marker-assisted breeding.