Crop Development Center

Lacombe, Canada

Crop Development Center

Lacombe, Canada
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Feng J.,Crop Diversification Center North | Hwang R.,University of Alberta | Chang K.F.,Crop Development Center | Hwang S.F.,Crop Diversification Center North | And 3 more authors.
Canadian Journal of Plant Pathology | Year: 2010

A rapid and efficient protocol for the extraction of genomic DNA from plant pathogenic fungi was developed. Key features of the protocol include the SDS-assisted lysis of fungal mycelium with inclusion of a glass bead to help break hyphal walls, followed by isopropanol precipitation of the DNA. The protocol was used to extract genomic DNA from a collection of 26 fungal species, representing many important plant pathogens. Yield of DNA ranged from 2.1-4.9 μg per 20 mg of mycelium or 0.4-0.6 μg per 20 mg of spores. The DNA was of sufficient purity to be digested by restriction enzymes, to serve as a template in the PCR-amplification of genomic fragments as large as 4.9 kb, and to be used in dot-blot hybridization for the detection of multiple- and single-copy genes. © 2010 The Canadian Phytopathological Society.

Feng J.,Crop Diversification Center North | Hwang R.,University of Alberta | Chang K.F.,Crop Development Center | Hwang S.F.,Crop Diversification Center North | And 4 more authors.
Plant Pathology | Year: 2010

Isolates of Fusarium spp. were recovered from the roots of field pea (Pisum sativum) collected from 15 commercial fields in Alberta, Canada. Most of the isolates (75 out of 96) were identified as F. avenaceum, based on morphology, phylogeny and species-specific PCR amplification. Molecular differences in the F. avenaceum isolates were detected based on putative mating type, and on ITS and CPN60 sequences. MAT-1 and MAT-2 were equally distributed among the isolates. Phylogenetic analysis based on ITS and CPN60 sequences clustered most of the F. avenaceum isolates into a single group. In some cases, isolates with low aggressiveness clustered together in additional groups. There was no correlation between phylogenetic profile and either mating type or geographic origin. This population of F. avenaceum has a low level of genetic variation and consists of isolates derived from the two mating types. Isolates with low aggressiveness are also retained in the population. © 2010 The Authors. Plant Pathology © 2010 BSPP.

Feng J.,Crop Diversification Center North | Hwang R.,University of Alberta | Chang K.F.,Crop Development Center | Conner R.L.,Agriculture and Agri Food Canada | And 5 more authors.
Canadian Journal of Plant Science | Year: 2011

Fusarium root rot, caused by Fusarium solani (Mart.) Sacc. f. sp. pisi (F. R. Jones) W. C. Snyder & H. N. Hans, is the most common root disease of field pea (Pisum sativum L.) in western Canada. In this study, a recombinant inbred line (RIL) population (n = 71) of field pea, derived from crosses between a resistant cultivar Carman, and a susceptible cultivar Reward, was evaluated to identify quantitative trait loci (QTL) controlling resistance to Fusarium root rot. The parental genotypes and RILs were evaluated for resistance to root rot following inoculation with F. solani in field experiments during 2007 and 2008. The frequency distribution of disease severities among the RILs was continuous. Transgressive segregation for resistance was observed among the RILs, with five lines more resistant than Carman, but no lines were more susceptible than Reward. To identify DNA markers linked with the resistance, 213 microsatellite markers were screened with genomic DNA from the two parental cultivars. Only 14 markers were polymorphic between the two parents and were used to genotype each of the RILs. Quantitative trait loci analysis based on the mean disease severity data from 2007 and 2008 identified a QTL that explained 39.0% of the phenotypic variance in the RIL population. This QTL is flanked by markers AA416 and AB60 on linkage group VII. The microsatellite markers that are closely linked to this QTL may be useful for marker assisted selection to develop cultivars with superior Fusarium root rot resistance.

Pozniak C.J.,Crop Development Center | Clarke J.M.,University of Saskatchewan
Canadian Journal of Plant Science | Year: 2015

CDC Carbide durum wheat is adapted to the durum production area of the Canadian prairies. This conventional-height durum wheat cultivar combines high grain yield potential with high grain pigment and protein concentrations, and low grain cadmium. CDC Carbide carries the Sm1 gene conferring resistance to the Orange Wheat Blossom Midge [Sitodiplosis modellana (Gehin)]. CDC Carbide is resistant to prevalent races of leaf, stem and stripe rust, and common bunt, and expresses end-use quality suitable for the Canada Western Amber Durum class. © 2015, Agricultural Institute of Canada. All rights reserved.

Hizbai B.T.,Agriculture and Agri Food Canada | Hizbai B.T.,University of Ottawa | Gardner K.M.,Agriculture and Agri Food Canada | Gardner K.M.,Dalhousie University | And 9 more authors.
Plant Genome | Year: 2012

Groat oil content and composition are important determinants of oat (Avena sativa L.) quality. We investigated these traits in a population of 146 recombinant inbred lines from a cross between 'Dal' (high oil) and 'Exeter' (low oil). A linkage map consisting of 475. Diversity Arrays Technology (DArT) markers spanning 1271.8 cM across 40 linkage groups was constructed. Quantitative trait locus (QTL) analysis for groat oil content and composition was conducted using grain samples grown at Aberdeen, ID, in 1997. Quantitative trait locus analysis for multiple agronomic traits was also conducted using data collected from hill plots and field plots in Ottawa, ON, in 2010. Using simple and composite interval mapping methods, QTLs for oil content, palmitic acid (16:0), stearic acid (18:0), oleic acid (18:1), linoleic acid (18:2), and linolenic acid (18:3) were identified. Two of the loci associated with oil content were associated with all of the fatty acids examined in this study, and most oil-related QTL showed similar patterns of effect on the fatty acid profile. These results suggest the presence of pleiotropic effects on oil-related traits through infl uences at specific nodes of the oil synthesis pathway. In addition, 12. QTL-associated markers (likely representing nine unique regions) were associated with plant height, heading date, lodging, and protein content. © Crop Science Society of America.

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