Crop Bioprotection Research Units

Peoria, IL, United States

Crop Bioprotection Research Units

Peoria, IL, United States
SEARCH FILTERS
Time filter
Source Type

Kim S.-J.,Crop Bioprotection Research Units | Kim S.-J.,South Korean National Institute of Animal Science | Dunlap C.A.,Crop Bioprotection Research Units | Kwon S.-W.,South Korean National Institute of Animal Science | Rooney A.P.,Crop Bioprotection Research Units
International Journal of Systematic and Evolutionary Microbiology | Year: 2015

Two independent isolates of a Gram-stain-positive, facultatively anaerobic, motile, rod-shaped bacterium were recovered from cheonggukjang, a Korean fermented soybean paste food product. Preliminary sequencing analysis of the 16S rRNA gene indicated that these strains were related most closely to Bacillus sonorensis KCTC-13918T and Bacillus licheniformis DSM 13T. In phenotypic characterization, the novel strains were found to grow between 15 and 55 °C and to tolerate up to 8% (w/v) NaCl. Furthermore, the strains grew in media of pH 5-10 (optimal growth at pH 7.0). The predominant cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0.The isoprenoid quinone was menaquinone 7 (MK-7). The cell-wall peptidoglycan contained meso-diaminopimelic acid. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unknown glycolipid. Draft genomes of the two strains were determined and in silico DNA-DNA hybridizations with their nearest neighbour (B. sonorensis KCTC-13918T) revealed 29.9% relatedness for both strains. Phylogenomic analysis of the genomes was conducted with the core genome (799 genes) of all strains in the Bacillus subtilis group and the two strains formed a distinct monophyletic cluster. In addition, the strains differed from the two most closely related species in that they did not metabolize maltose, D-galactose, D-sorbitol or D-gluconic acid. The DNA G + C content was 45.9 mol%. Based upon the consensus of phylogenetic and phenotypic analyses, these strains represent a novel species of the genus Bacillus, for which the name Bacillus glycinifermentans sp. nov. is proposed. The type strain is GO-13T (=KACC 18425T=NRRL B-65291T). © 2015 IUMS.


Dunlap C.A.,Crop Bioprotection Research Units | Kim S.-J.,Crop Bioprotection Research Units | Kim S.-J.,South Korean National Institute of Animal Science | Kwon S.-W.,South Korean National Institute of Animal Science | Rooney A.P.,Crop Bioprotection Research Units
International Journal of Systematic and Evolutionary Microbiology | Year: 2015

The rhizosphere-isolated bacteria belonging to the Bacillus amyloliquefaciens subsp. plantarumand Bacillus methylotrophicus clades are an important group of strains that are used as plant growth promoters and antagonists of plant pathogens. These properties have made these strains the focus of commercial interest. Here, we present the draft genome sequence of B. methylotrophicus KACC 13105T (=CBMB205T). Comparative genomic analysis showed only minor differences between this strain and the genome of the B. amyloliquefaciens subsp.plantarum type strain, with the genomes sharing approximately 95% of the same genes. The results of morphological, physiological, chemotaxonomic and phylogenetic analyses indicate that the type strains of these two taxa are highly similar. In fact, our results show that the type strain of B. amyloliquefaciens subsp. plantarum FZB42T (=DSM 23117T=BGSC 10A6T) does not cluster with other members of the B. amyloliquefaciens taxon. Instead, it clusters well within a clade of strains that are assigned to B. methylotrophicus, including the type strain of that species. Therefore, we propose that the subspecies B. amyloliquefaciens subsp. plantarum should be reclassified as a later heterotypic synonym of B. methylotrophicus. © 2015 IUMS.


Dunlap C.A.,Crop Bioprotection Research Units
International Journal of Systematic and Evolutionary Microbiology | Year: 2015

‘Bacillus vanillea’ XY18 (=CGMCC 8629=NCCB 100507) was isolated from cured vanilla beans and involved in the formation of vanilla aroma compounds. A draft genome of this strain was assembled and yielded a length of 3.71 Mbp with a DNA G + C content of 46.3 mol%. Comparative genomic analysis with its nearest relatives showed only minor differences between this strain and the genome of the Bacillus siamensis KCTC 13613T (=BCC 22614T=KACC 16244T), with a calculated DNA–DNA hybridization (DDH) value of 91.2% and an average nucleotide identity (ANI) of 98.9%. This DDH value is well above the recommended 70% threshold for species delineation, as well as the ANI threshold of 95%. In addition, the results of morphological, physiological, chemotaxonomic and phylogenetic analyses indicate that the type strains of these two taxa are highly similar with phenotype coherence. A core genome multi-locus sequencing analysis was conducted for the strains and the results show that ‘Bacillus vanillea’ XY18 clusters closely to the type strain of Bacillus siamensis. Therefore, it is proposed that the species ‘Bacillus vanillea’ XY18 (=CGMCC 8629=NCCB 100507) should be reclassified as a later heterotypic synonym of Bacillus siamensis KCTC 13613T (=BCC 22614T=KACC 16244T). An emended description of Bacillus siamensis is provided. © 2015 IUMS.


PubMed | Crop Bioprotection Research Units
Type: Journal Article | Journal: International journal of systematic and evolutionary microbiology | Year: 2016

Bacillus vanillea XY18 (=CGMCC 8629=NCCB 100507) was isolated from cured vanilla beans and involved in the formation of vanilla aroma compounds. A draft genome of this strain was assembled and yielded a length of 3.71Mbp with a DNA G+C content of 46.3mol%. Comparative genomic analysis with its nearest relatives showed only minor differences between this strain and the genome of the Bacillus siamensis KCTC 13613T (=BCC 22614T=KACC 16244T), with a calculated DNADNA hybridization (DDH) value of 91.2% and an average nucleotide identity (ANI) of 98.9%. This DDH value is well above the recommended 70% threshold for species delineation, as well as the ANI threshold of 95%. In addition, the results of morphological, physiological, chemotaxonomic and phylogenetic analyses indicate that the type strains of these two taxa are highly similar with phenotype coherence. A core genome multi-locus sequencing analysis was conducted for the strains and the results show that Bacillus vanillea XY18 clusters closely to the type strain of Bacillus siamensis. Therefore, it is proposed that the species Bacillus vanillea XY18 (=CGMCC 8629=NCCB 100507) should be reclassified as a later heterotypic synonym of Bacillus siamensis KCTC 13613T (=BCC 22614T=KACC 16244T). An emended description of Bacillus siamensis is provided.

Loading Crop Bioprotection Research Units collaborators
Loading Crop Bioprotection Research Units collaborators