Croatian Food Agency

Osijek, Croatia

Croatian Food Agency

Osijek, Croatia
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Pleadin J.,Croatian Veterinary Institute | Bevardi M.,Dr Andrija Stampar Institute Of Public Health | Sokolic-Mihalak D.,Croatian Food Agency
Food Control | Year: 2013

When domestic animals are exposed to mycotoxins, significant amounts of the latter shall be carried over into animal products such as milk, eggs and meat. This study was carried out in order to determine the possible presence of aflatoxin B1 (AFB1), ochratoxin A (OTA) and citrinin (CIT) in game sausages (n=15), semi-dry sausages (n=25) and fermented dry-meat products (n=50), randomly taken from individual producers and the Croatian market. AFB1 and OTA were quantified using ELISA, while CIT was quantified using HPLC-fluorescence detector. Out of 90 samples, the fungi most frequently isolated from dry-cured meat products were of Penicillium species, while Aspergillus was isolated from only one sample. As much as 68.88% of the samples were positive for mycotoxins. Finally, the analysis of different types of meat products resulted in OTA identification in 64.44%, CIT identification in 4.44% and AFB1 identification in 10% of the samples. The maximum OTA concentrations established in the commercial sausage samples equalled to 7.83μg/kg, while that of AFB1 amounted to 3.0μg/kg. Generally, although OTA was detected in all three types of products in different percentage shares, mutual differences were not statistically significant (P>0.05). © 2013 Elsevier Ltd.

Vulic A.,Croatian Veterinary Institute | Hengl B.,Croatian Food Agency | Gross-Boskovic A.,Croatian Food Agency | Jurkovic M.,Croatian Food Agency | And 2 more authors.
Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment | Year: 2016

Ochratoxin A (OTA) is a toxic secondary metabolite produced by the fungi of Aspergillus and Penicillium species. Data indicate a frequent OTA contamination of cereals and cereal products, and consequently also the contamination of meat and meat products. The aim of this study was to determine a possible level of meat product consumers’ exposure to OTA through the consumption of dry-cured and fermented meat products available on the Croatian market. Data showed the weekly OTA intake of 90% of male dry-cured ham consumers to be a maximum of 51.9 ng kg–1 b.w., i.e., far below the tolerable weekly intake (TWI) of 120 ng kg–1 b.w. weekly set out by the European Food Safety Authority (EFSA). OTA intake coming from the consumption of other meat products under study is lower and ranges from 0.1 to 42.1 ng kg–1 b.w. weekly, dependent on the study. The study demonstrated that meat products in Croatia do not constitute a notable source of OTA in the human diet, so that the human health risk coming from the consumption of dry-cured and fermented meat products is negligible. © 2016 Informa UK Limited, trading as Taylor & Francis Group

Sokolic-Mihalak D.,Croatian Food Agency | Frece J.,University of Zagreb | Slavica A.,University of Zagreb | Delas F.,University of Zagreb | And 2 more authors.
Arhiv za Higijenu Rada i Toksikologiju | Year: 2012

The aim of this study was to determine the effects of the essential oil of Thymus serpyllum L. and of its components thymol and total phenols (total phenolic content, TPC) extracted from the plant on the growth and mycotoxin production of Aspergillus ochraceus, A. carbonarius, and A. niger. Minimal inhibitory concentration (MIC) determined for the essential oil and thymol, and selected concentration of the TPC extract inhibited fungal growth and ochratoxin A biosynthesis by more than 60 %, depending on the conditions and duration of incubation with the fungi. Essential oil showed the strongest inhibitory effect which may have been related to the synergistic or cumulative effects of its components.

Strelec I.,Josip Juraj Strossmayer University of Osijek | Komlenic D.K.,Josip Juraj Strossmayer University of Osijek | Jurkovic V.,Josip Juraj Strossmayer University of Osijek | Jurkovic Z.,Croatian Food Agency | Ugarcic-Hardi Z.,Josip Juraj Strossmayer University of Osijek
Agriculturae Conspectus Scientificus | Year: 2010

Changes in seed quality parameter of three wheat varieties during one year storage at four diff erent storage conditions, were studied. Applied storage conditions adversely aff ected quality changes in wheat seeds during one year storage. The most pronounced changes were observed for seeds kept at 40°C, RH = 45%, followed by seeds stored at 25°C, RH = 45%, while seeds kept at 4°C, RH = 45% or at warehouse conditions mostly showed minimal or statistically insignifi cant changes. Elevated temperature of seed storage caused a signifi cant decrease of starch content, hectolitre weight, and wet gluten content, accompanied with increase in flour acidity, and fl uctuating in Zeleny sedimentation value. The intensities of observed changes showed strong dependence on wheat variety.

JurkoviC M.,Croatian Food Agency | Sokolic D.,Croatian Food Agency | Basic S.,Croatian Food Agency | Gross-Boskovic A.,Croatian Food Agency | Kenjeric D.,Josip Juraj Strossmayer University of Osijek
Journal of Food and Nutrition Research | Year: 2015

Data from National Food Consumption Survey were used to assess intake of selected vitamins and minerals from fresh fruit in adult population. A number of 165 adults from the Zagreb region in Croatia were selected for this purpose. To assess dietary intake, the 24-hour recall method was used and participants were separated according to age in 3 groups (19-30 years, 31-50 years and 51-64 years), as well as according to gender. Data are presented as the average daily intake and compared to the Dietary Reference Intakes (DRI) values. The results showed that, in male population, intake of Na, K, P, Fe and Zn was higher in older population, while intake of Ca and Cu was higher in middle-aged population. Intake of vitamins in male population for thiamin and pyridoxine was higher in middle-aged population, and intake of riboflavin and niacin was higher in older population. Vitamin C intake was lower and similar in middle-aged and older population, respectively, compared to the youngest population. Regarding female popula- tion, the highest intake of almost all vitamins and minerals was in the youngest population, except for sodium and iron. Significant differences in daily intake of minerals and vitamins were observed between female subgroups. © 2015 National Agricultural and Food Centre (Slovakia).

Jurkovic M.,Croatian Food Agency | Marijanovic-Vincetic D.,Croatia and Slavonia nova | Jurkovic Z.,Osijek Agricultural Institute | Mandic M.L.,Josip Juraj Strossmayer University of Osijek | Sokolic-Mihalak D.,Croatian Food Agency
Proceedings of the 7th International Congress FLOUR-BREAD 2013 - 9th Croatian Congress of Cereal Technologists | Year: 2013

The reduction of salt intake is a public health issue in many countries because it has been identified as the main cause of several diseases such as hypertension, heart and kidney diseases, stomach cancer, osteoporosis, stroke and obesity. The WHO has set a worldwide target of a maximum salt intake of 5 g/day for adults as a recommendation to reduce daily salt intake. Of all foodstuffs, bread has been identified as the single highest contributor to the total daily salt intake. Therefore, the aim of this paper was to estimate salt intake through bakery products in Slavonia Region and their dependency on different parameters. The quantity of bakery product intake derived from national food consumption survey. The results showed that consumers in Slavonia eat about 148 g/day of various bakery products, and consequently intake 2.43 g/day of salt, which is half of the recommended daily intake. Research has shown that salt intake by bakery products are almost the same in villages and towns. Related to gender, men's intake is higher than women. The intake is highest at low body mass index, and is higher in younger than in elderly population.

Viljevac M.,Osijek Agricultural Institute | Dugalic K.,Osijek Agricultural Institute | Mihaljevic I.,Osijek Agricultural Institute | SImic D.,Osijek Agricultural Institute | And 4 more authors.
Acta Botanica Croatica | Year: 2013

Drought is a limiting factor in fruit production today. Identification of sour cherry genotypes tolerant to drought will enable the sustainability of fruit production. The aim of our study was to select sour cherry genotypes according to their genetic background as well as drought tolerance and investigate possible mechanisms of drought tolerance through the changes in photosynthetic apparatus (i.e. photosynthetic pigment content) and photosynthesis process assessed through the chlorophyll fluorescence transient. All of them together with molecular markers (SSRs and AFLPs), relative water content (RWC) as indicator of plant water status distinguish two genotypes (Kelleris 16 and OS), which are the opposite in regards to drought tolerance. Down-regulation of photosynthesis in drought-treated Kelleris 16 plants was seen as changes in antenna complexes of PSII (decreased total chlorophylls content (a+b) and chlorophylls ratio (a/b)). Despite unchanged maximum quantum yield of PSII in drought-treated leaves of genotype OS, overall photosynthetic performance expressed as PIABS was down-regulated in both investigated genotypes. However, decrement of PIABS was much pronounced in genotype Kelleris 16, mainly because of changes in a certain fraction of RCs, which become dissipative centres, seen as increase in ABS/RC and DI0/RC, in order to avoid photooxidative damage of photosynthetic apparatus. Also, electron transport, seen as decrease in ET0/ (TR0-ET0) and ET0/RC, was impaired which lead to impaired CO2 fixation and photosynthesis. The described changes in the functioning of photosynthetic apparatus in drought-treated plants of Kelleris 16 constitute the main distinction between the two investigated genotypes regarding drought adaptation mechanisms. © 2013 by Acta Botanica Croatica.

Bilandzic N.,Croatian Veterinary Institute | Kolanovic B.S.,Croatian Veterinary Institute | Varenina I.,Croatian Veterinary Institute | Jurkovic Z.,Croatian Food Agency
Mljekarstvo | Year: 2011

A total of 119 raw milk samples collected at individual small milk-producing facilities and collection tanks of milk routes from five counties of east and north continental Croatia were examined for chloramphenicol, sulfonamides, tetracyclines, gentamicin, streptomycin, dihydrostreptomycin, flumequine and enrofloxacin from January to March of 2011. Immunoassay methods used for drug determination were validated according to the guidelines laid down by European Commission Decision 2002/657/EC. Data indicated that the methods are appropriate for the detection of antibiotics measured. Measured mean values (μg L -1) of antibiotics were: 0.005 for chloramphenicol, 3.67 for sulfonamides, 2.83 for tetracyclines, 1.10 for gentamicin, 2.64 for streptomycin, 7.67 for dihydrostreptomycin, 10.4 for flumequine and 4.11 for enrofloxacin. None of samples analyzed showed the presence of veterinary drug residues above the maximum residues levels (MRLs) established by European Union and Croatian legislation. The calculated estimated daily intakes (EDIs) for the average daily milk consumption of 300 mL for an adult in Croatia for examined antibiotics showed levels 20 to 1640 times lower than the values of acceptable daily intakes (ADIs) fixed by European Medicines Agency and World Health Organization. This suggested that toxicological risk associated with the consumption of analysed milk could not be considered a public health issue with regards to these veterinary drugs.

PubMed | Croatian Food Agency
Type: Journal Article | Journal: Arhiv za higijenu rada i toksikologiju | Year: 2013

The aim of this study was to determine the effects of the essential oil of Thymus serpyllum L. and of its components thymol and total phenols (total phenolic content, TPC) extracted from the plant on the growth and mycotoxin production of Aspergillus ochraceus, A. carbonarius, and A. niger. Minimal inhibitory concentration (MIC) determined for the essential oil and thymol, and selected concentration of the TPC extract inhibited fungal growth and ochratoxin A biosynthesis by more than 60 %, depending on the conditions and duration of incubation with the fungi. Essential oil showed the strongest inhibitory effect which may have been related to the synergistic or cumulative effects of its components.

Tucak M.,Osijek Agricultural Institute | Popovic S.,Osijek Agricultural Institute | Cupic T.,Osijek Agricultural Institute | Grljusic S.,Osijek Agricultural Institute | And 3 more authors.
Russian Journal of Genetics | Year: 2010

Information on genetic diversity and germplasm characterization is essential for successful crop improvement. Diverse data sets (pedigree, morphological, biochemical, DNA based-markers) are employed in various aspects of plant analysis. The objective of this study was to determine the efficiency of phenotypic and RAPD markers in diversity assessment of ten alfalfa (Medicago spp.) accessions from Europe, North America and Australia. Field experiment was designed as a randomised complete block with three replications over two consecutive years (2004, 2005) at one location. Twelve morpho-agronomic traits were recorded on 50 plants per each accession. Genomic DNA's from 16-20 randomly selected individual plants per accession were used for RAPD analysis. Six primers selected in this study generated a total of 93 polymorphic RAPD bands. The number of polymorphic bands detected per primer ranged from 11 to 20. Genetic distances (GD) among investigated accessions and two-dimensional principal coordinate analysis (2D PCoA) based on phenotypic and molecular data were obtained. The average GD between (0. 283-0. 416) and within (0. 247-0. 332) accessions based on RAPD data was higher than GD values obtained by morpho-agronomic traits (0. 171-0. 354 and 0. 157-0. 261, respectively). 2D PCoA based on GD from RAPD data grouped most of the studied individual plants to four clusters according to their geographical or taxonomy origin. 2D PCoA based only on morpho-agronomic data did not group plants congruently to their origin, probably due to a strong environmental influence on studied traits. Our results indicated that the RAPD markers were effective in assessing genetic diversity within and between studied alfalfa accessions. In addition, the obtained results suggested that the RAPD markers might be useful for grouping of germplasm with similar genetic background and for pre-screening of potential heterotic groups in our breeding programme. © 2010 Pleiades Publishing, Ltd.

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