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Osijek, Croatia

Strelec I.,Josip Juraj Strossmayer University of Osijek | Komlenic D.K.,Josip Juraj Strossmayer University of Osijek | Jurkovic V.,Josip Juraj Strossmayer University of Osijek | Jurkovic Z.,Croatian Food Agency | Ugarcic-Hardi Z.,Josip Juraj Strossmayer University of Osijek
Agriculturae Conspectus Scientificus | Year: 2010

Changes in seed quality parameter of three wheat varieties during one year storage at four diff erent storage conditions, were studied. Applied storage conditions adversely aff ected quality changes in wheat seeds during one year storage. The most pronounced changes were observed for seeds kept at 40°C, RH = 45%, followed by seeds stored at 25°C, RH = 45%, while seeds kept at 4°C, RH = 45% or at warehouse conditions mostly showed minimal or statistically insignifi cant changes. Elevated temperature of seed storage caused a signifi cant decrease of starch content, hectolitre weight, and wet gluten content, accompanied with increase in flour acidity, and fl uctuating in Zeleny sedimentation value. The intensities of observed changes showed strong dependence on wheat variety. Source

Sokolic-Mihalak D.,Croatian Food Agency | Frece J.,University of Zagreb | Slavica A.,University of Zagreb | Delas F.,University of Zagreb | And 2 more authors.
Arhiv za Higijenu Rada i Toksikologiju | Year: 2012

The aim of this study was to determine the effects of the essential oil of Thymus serpyllum L. and of its components thymol and total phenols (total phenolic content, TPC) extracted from the plant on the growth and mycotoxin production of Aspergillus ochraceus, A. carbonarius, and A. niger. Minimal inhibitory concentration (MIC) determined for the essential oil and thymol, and selected concentration of the TPC extract inhibited fungal growth and ochratoxin A biosynthesis by more than 60 %, depending on the conditions and duration of incubation with the fungi. Essential oil showed the strongest inhibitory effect which may have been related to the synergistic or cumulative effects of its components. Source

Viljevac M.,Osijek Agricultural Institute | Dugalic K.,Osijek Agricultural Institute | Mihaljevic I.,Osijek Agricultural Institute | SImic D.,Osijek Agricultural Institute | And 4 more authors.
Acta Botanica Croatica | Year: 2013

Drought is a limiting factor in fruit production today. Identification of sour cherry genotypes tolerant to drought will enable the sustainability of fruit production. The aim of our study was to select sour cherry genotypes according to their genetic background as well as drought tolerance and investigate possible mechanisms of drought tolerance through the changes in photosynthetic apparatus (i.e. photosynthetic pigment content) and photosynthesis process assessed through the chlorophyll fluorescence transient. All of them together with molecular markers (SSRs and AFLPs), relative water content (RWC) as indicator of plant water status distinguish two genotypes (Kelleris 16 and OS), which are the opposite in regards to drought tolerance. Down-regulation of photosynthesis in drought-treated Kelleris 16 plants was seen as changes in antenna complexes of PSII (decreased total chlorophylls content (a+b) and chlorophylls ratio (a/b)). Despite unchanged maximum quantum yield of PSII in drought-treated leaves of genotype OS, overall photosynthetic performance expressed as PIABS was down-regulated in both investigated genotypes. However, decrement of PIABS was much pronounced in genotype Kelleris 16, mainly because of changes in a certain fraction of RCs, which become dissipative centres, seen as increase in ABS/RC and DI0/RC, in order to avoid photooxidative damage of photosynthetic apparatus. Also, electron transport, seen as decrease in ET0/ (TR0-ET0) and ET0/RC, was impaired which lead to impaired CO2 fixation and photosynthesis. The described changes in the functioning of photosynthetic apparatus in drought-treated plants of Kelleris 16 constitute the main distinction between the two investigated genotypes regarding drought adaptation mechanisms. © 2013 by Acta Botanica Croatica. Source

Popovic R.,Osijek Agricultural Institute | Ivanisic I.,Osijek Agricultural Institute | Jurkovic V.,Osijek Agricultural Institute | Jurkovic Z.,Croatian Food Agency
Poljoprivreda | Year: 2010

Changes in grain moisture, germination and vigour of three wheat cultivars packed in paper bags and stored for one year under four different conditions of environmental temperature and relative humidity (RH) were investigated. During the first ninety days of storage significant reduction in grain moisture content of 4, 2.5 and 0.9 %, respectively, under 40 °C, 25 °C and 4 °C and RH of 45 % occurred. Subsequently grain moisture remained constant until the end of storage. Seeds of examined cultivars lost their germination ability and vigour only under elevated storage temperatures. Germination and vigour loss after one year of storage differed between cultivars being higher for seeds kept under 40°C, RH = 45 % (35-85% and 55-94 %, respectively), than under 25°C, RH = 45 % (10-20 % and 15-22 %, respectively). Obtained data indicate significant influence of storage conditions on moisture content, germination and vigour changes during storage of wheat seeds, as wellas varietal dependence of seed viability. Source

Tucak M.,Osijek Agricultural Institute | Popovic S.,Osijek Agricultural Institute | Cupic T.,Osijek Agricultural Institute | Grljusic S.,Osijek Agricultural Institute | And 3 more authors.
Russian Journal of Genetics | Year: 2010

Information on genetic diversity and germplasm characterization is essential for successful crop improvement. Diverse data sets (pedigree, morphological, biochemical, DNA based-markers) are employed in various aspects of plant analysis. The objective of this study was to determine the efficiency of phenotypic and RAPD markers in diversity assessment of ten alfalfa (Medicago spp.) accessions from Europe, North America and Australia. Field experiment was designed as a randomised complete block with three replications over two consecutive years (2004, 2005) at one location. Twelve morpho-agronomic traits were recorded on 50 plants per each accession. Genomic DNA's from 16-20 randomly selected individual plants per accession were used for RAPD analysis. Six primers selected in this study generated a total of 93 polymorphic RAPD bands. The number of polymorphic bands detected per primer ranged from 11 to 20. Genetic distances (GD) among investigated accessions and two-dimensional principal coordinate analysis (2D PCoA) based on phenotypic and molecular data were obtained. The average GD between (0. 283-0. 416) and within (0. 247-0. 332) accessions based on RAPD data was higher than GD values obtained by morpho-agronomic traits (0. 171-0. 354 and 0. 157-0. 261, respectively). 2D PCoA based on GD from RAPD data grouped most of the studied individual plants to four clusters according to their geographical or taxonomy origin. 2D PCoA based only on morpho-agronomic data did not group plants congruently to their origin, probably due to a strong environmental influence on studied traits. Our results indicated that the RAPD markers were effective in assessing genetic diversity within and between studied alfalfa accessions. In addition, the obtained results suggested that the RAPD markers might be useful for grouping of germplasm with similar genetic background and for pre-screening of potential heterotic groups in our breeding programme. © 2010 Pleiades Publishing, Ltd. Source

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