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Keresa S.,University of Zagreb | Bosnjak A.M.,University of Zagreb | Baric M.,University of Zagreb | Jercic I.H.,University of Zagreb | And 2 more authors.
Notulae Botanicae Horti Agrobotanici Cluj-Napoca | Year: 2012

'Topaz' is a modern Czech apple cultivar well accepted by consumers and scab-resistant, providing reasons for the significant spread of cv. 'Topaz' in European orchards, especially in the organic fruit production industry. Growing the apple trees on their own roots provides some advantages in comparison with grafted trees. Micropropagation is the method of choice for plantlet production for this purpose as well as for the establishment of healthy mother stock trees as a source of scions. The efficiency of axillary shoot proliferation was examined on four media differing in plant growth regulators and their concentrations, and from three explant types: intact or decapitated and defoliated microshoots placed vertically and one-nodal segments placed horizontally. All media consisted of Quoirin and Lepoivre (QL) macroelements and Murashige and Skoog (MS) microelements. Furthermore, rooting efficiency on six different media/treatments was analyzed. Media with 1 mg/L 6-benzylaminopurine (BA) or BA (0.5 mg/L) + 1.5 mg/L kinetin (Kin) produced similar number of microshoots per inoculated one (2.5 and 2.4, respectively). Medium with 1 mg/L thidiazuron (TDZ) produced significantly higher number of shoots (3.6) but they were fasciated. Three different explant types also produced similar numbers of microshoots. High rooting efficiency (68.7%), a high number of roots per shoot (6.6) and the best quality of shoots were obtained in rooting medium containing 2 mg/L of indole-3-butyric acid (IBA). An efficient method of shoot proliferation was established, and, since rooting was the most critical step, an efficient procedure for rooting apple cv. 'Topaz' was established.


Pecek G.,Croatian Center for Agriculture | Pavlovic D.M.,Marulicev trg | Babic S.,Marulicev trg
International Journal of Environmental Analytical Chemistry | Year: 2013

A method for analysis of 20 commonly used pesticides in surface water based on solid-phase extraction and gas chromatography-mass spectrometry was proposed. During method development the key parameters that can affect SPE extraction and determination such as selection of efficient SPE sorbent, pH of water sample, type and volume of elution solvent, breakthrough volume and matrix effects were investigated. The method was validated using spring water spiked with appropriate concentration of pesticides. The obtained correlation coefficients were in range 0.9972-1.000, limits of detection (LOD) were 0.001-0.5 μg L-1 and the limits of quantification (LOQ) were 0.005-1 μg L-1 depending on a pesticide. Much higher LOD (20 μg L-1) and LOQ (50 μg L-1) values were obtained for bentazone. The influence of matrix was assessed using real water samples spiked with appropriate concentration of pesticide standards solution. Both signal enhancement and suppression were observed, depending on a pesticide, therefore standard addition method was used for pesticides determination. The developed method was applied on real water samples taken in close vicinity of agricultural fields. Many of the targeted pesticides were found in the samples and the results are presented in this article. © 2013 Copyright Taylor and Francis Group, LLC.


Bisko A.,Croatian Center for Agriculture | Cosic T.,University of Zagreb | Jelaska S.,University of Zagreb
Agriculturae Conspectus Scientificus | Year: 2010

The study aimed at analysing the reaction of three strawberry Fragaria x ananassa Duch. cultivars to the salinity (0-control, 0.5, 1.0, 2.0, 3.0 and 4.0 g of NaCl L-1) in the irrigation water: equivalent of the following EC values: (0.73, 1.65, 2.66, 4.37, 5.93 and 7.81 mS cm-1). Vegetative trial was carried out at the Faculty of Agriculture in Zagreb during 1997 and 1998. Following parameters were tested: degrees of damage of shoots, number of leaves, number of runners (stolons), yield of fresh biomass and dry matter of shoots and roots, and percentage of dry matter of shoots and roots. Tested cultivars showed signifi cant diff erences of the level of damage (necrosis and decay of plants due to high NaCl concentration). Th e highest degree of damage was shown by 'Elsanta' in both trial years, followed by 'Marmolada' and 'Miranda' respectively. Th e analysis of the number of leaves and stolons confi rmed the same trend. Th e yield of fresh biomass and dry matter of shoots and roots was signifi cantly reduced with the application of NaCl. 'Miranda' was characterised by the highest fresh biomass and dry matter yield, followed by 'Marmolada' and 'Elsanta'. Average decrease of fresh biomass yield of shoots in comparison to the control was 45, 33 and 22% in 'Elsanta', 'Marmolada' and 'Miranda'. Dry matter yield of shoots decreased correspondingly for 34, 28 and 18%. Total dry matter yield (shoots + roots) of 'Elsanta' was signifi cantly reduced at 0.5 g NaCl L-1 and of 'Marmolada' and 'Miranda' at 1.0 and 2.0 g L-1 respectively.


Masten Milek T.,Croatian Center for Agriculture | Simala M.,Institute for Plant Protection | Pintar M.,Institute for Plant Protection
EPPO Bulletin | Year: 2016

In recent years, several species of alien scale insects have arrived and spread rapidly through European and Mediterranean countries. One hundred and twenty-nine species of alien scale insects have so far been recorded in Europe. This paper presents a list of alien scale insect species for Croatia, comprising 56 species from 8 different families. Four of them are new records for Croatia: Aonidiella taxus Leonardi, 1906, Ichnaspis longirostris (Signoret, 1882), Phoenicococcus marlatii (Cockerell, 1899) and Spilococcus mammillariae (Bouche, 1844). The majority of species recorded belong to the following families: Diaspididae (28 species), Coccidae (12 species), Pseudococcidae (10 species), Eriococcidae (two species). The scale insect families Dactylopidae, Margarodidae, Ortheziidae and Pheniococcidae comprised only one recorded alien species each. During the time frame covered in the review (from the beginning of the 20th century until 2015), the majority of alien scale insect species (23) were registered in the period from 2000 to 2015. Data on the origin of introduced alien scale insects shows that the majority of the species originate from Asia (22 species), followed by North America (6 species), Africa, Australia and Central America (5 species each) and South America (4 species), with 9 species being of unknown origin. © 2016 The Authors. Journal compilation © 2016 OEPP/EPPO


In this study in vitro pollen viability and germination in intact pollens and pollen tube growth in germinated pollens of five common pomegranate cultivars grown in Croatia and Bosnia and Herzegovina was investigated first time. Pollen viability varied from 36.73% (cv. Konjski Zub) to 51.80% (cv. Barski) in fluorescent diacetat (FDA) test. The average pollen germination percentages were found as the lowest 6.83% in cv. Konjski Zub and the highest 42.51% in cv Glavas. Among the germination media in general 0.2 agar + 10% sucrose+5 ppm H 3BO 3 gave better results to obtain higher pollen germination for all cultivars. In germinated pollens of these five cultivars, significant differences were observed on pollen tube length. Average pollen tube lengths in germinated pollens of five cultivars were measured as minimum 302 μm in cv. Konjski Zub and as maximum 344 μm in cv. Ciparski. The results showed that there were obvious differences in pollen germinability among cultivars growing under the same environmental conditions and these differences must be considered for orchard establishment to obtain higher yield.

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