Croatian Center for Agriculture

Zagreb, Croatia

Croatian Center for Agriculture

Zagreb, Croatia
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Simala M.,Institute for Plant Protection Rim 98 | Milek T.M.,Croatian Center for Agriculture | Pintar M.,Institute for Plant Protection Rim 98
Natura Croatica | Year: 2015

A check list of whiteflies in Croatia contains 31 species. This is the result of browsing the literature of faunistic investigations of whiteflies in Croatia from 1940 to 2007 and faunistic research done by the authors in the period 2005-2007. During faunistic investigations, 7 of the total 31 recorded species were whitefly species with dark puparia and pupal cases. These are the following species: Aleuroclava hikosanensis Takahashi 1938, Aleurolobus olivinus Silvestri 1911, Aleurolobus wunni Ryberg 1938, Aleurotuba jelinekii Frauenfeld 1867, Aleuroviggianus adrianae Iaccarino 1982, Simplaleurodes hemisphaerica Goux 1945 and Tetralicia ericae Harrison 1917. In addition to these, another 2 whitefly species with dark puparia and pupal cases were recorded later in Croatia. These are Aleurocanthus spiniferus (Quaintance 1903), which was reported for the first time in Croatia in 2012 and Aleuroclava aucubae (Kuwana 1911), first detected in Croatia in 2013. © 2015, Croatian Natural History Museum. All rights reserved.


Lemic D.,University of Zagreb | Mikac K.M.,University of Wollongong | Kozina A.,Croatian Center for Agriculture | Benitez H.A.,University of Tarapacá | And 2 more authors.
Pest Management Science | Year: 2015

BACKGROUND: The western corn rootworm (WCR) is economically the most important pest of maize in Croatia. To predict WCR adult population abundance and variability, traditional, genetic and morphometric monitoring of populations was conducted over time through each phase of the WCR invasion process in Croatia. RESULTS: Through traditional monitoring it was shown that WCR established their current population and reached economic densities after 14 years persisting in the study area. Regression-tree-based modelling showed that the best predictor of WCR adult abundance was the total amount of rainfall. Genetic monitoring indicated that genetic differentiation increased over time at the intrapopulation level, and morphometric monitoring indicated that wing morphotypes varied according to edaphic landscape changes. CONCLUSION: Traditional population metric surveys are important in WCR integrated pest management (IPM), as such surveys can be effectively used to predict population abundances. Novel-use monitoring techniques such as genetics and geometric morphometrics can be used to provide valuable information on variation within and among populations. The monitoring techniques presented herein provide sound data to assist in the understanding of both WCR ecology and population genetics and may provide more information than that currently available using traditional techniques (e.g. sticky traps), and as such these additional techniques should be written into IPM for WCR. © 2015 Society of Chemical Industry.


Keresa S.,University of Zagreb | Bosnjak A.M.,University of Zagreb | Baric M.,University of Zagreb | Jercic I.H.,University of Zagreb | And 2 more authors.
Notulae Botanicae Horti Agrobotanici Cluj-Napoca | Year: 2012

'Topaz' is a modern Czech apple cultivar well accepted by consumers and scab-resistant, providing reasons for the significant spread of cv. 'Topaz' in European orchards, especially in the organic fruit production industry. Growing the apple trees on their own roots provides some advantages in comparison with grafted trees. Micropropagation is the method of choice for plantlet production for this purpose as well as for the establishment of healthy mother stock trees as a source of scions. The efficiency of axillary shoot proliferation was examined on four media differing in plant growth regulators and their concentrations, and from three explant types: intact or decapitated and defoliated microshoots placed vertically and one-nodal segments placed horizontally. All media consisted of Quoirin and Lepoivre (QL) macroelements and Murashige and Skoog (MS) microelements. Furthermore, rooting efficiency on six different media/treatments was analyzed. Media with 1 mg/L 6-benzylaminopurine (BA) or BA (0.5 mg/L) + 1.5 mg/L kinetin (Kin) produced similar number of microshoots per inoculated one (2.5 and 2.4, respectively). Medium with 1 mg/L thidiazuron (TDZ) produced significantly higher number of shoots (3.6) but they were fasciated. Three different explant types also produced similar numbers of microshoots. High rooting efficiency (68.7%), a high number of roots per shoot (6.6) and the best quality of shoots were obtained in rooting medium containing 2 mg/L of indole-3-butyric acid (IBA). An efficient method of shoot proliferation was established, and, since rooting was the most critical step, an efficient procedure for rooting apple cv. 'Topaz' was established.


Gadze J.,University of Zagreb | Radunic M.,Institute for Adriatic Crops | Petric I.V.,Croatian Center for Agriculture | Ercisli S.,Atatürk University
Acta Scientiarum Polonorum, Hortorum Cultus | Year: 2011

In this study in vitro pollen viability and germination in intact pollens and pollen tube growth in germinated pollens of five common pomegranate cultivars grown in Croatia and Bosnia and Herzegovina was investigated first time. Pollen viability varied from 36.73% (cv. Konjski Zub) to 51.80% (cv. Barski) in fluorescent diacetat (FDA) test. The average pollen germination percentages were found as the lowest 6.83% in cv. Konjski Zub and the highest 42.51% in cv Glavas. Among the germination media in general 0.2 agar + 10% sucrose+5 ppm H 3BO 3 gave better results to obtain higher pollen germination for all cultivars. In germinated pollens of these five cultivars, significant differences were observed on pollen tube length. Average pollen tube lengths in germinated pollens of five cultivars were measured as minimum 302 μm in cv. Konjski Zub and as maximum 344 μm in cv. Ciparski. The results showed that there were obvious differences in pollen germinability among cultivars growing under the same environmental conditions and these differences must be considered for orchard establishment to obtain higher yield.


Bisko A.,Croatian Center for Agriculture | Cosic T.,University of Zagreb | Jelaska S.,University of Zagreb
Agriculturae Conspectus Scientificus | Year: 2010

The study aimed at analysing the reaction of three strawberry Fragaria x ananassa Duch. cultivars to the salinity (0-control, 0.5, 1.0, 2.0, 3.0 and 4.0 g of NaCl L-1) in the irrigation water: equivalent of the following EC values: (0.73, 1.65, 2.66, 4.37, 5.93 and 7.81 mS cm-1). Vegetative trial was carried out at the Faculty of Agriculture in Zagreb during 1997 and 1998. Following parameters were tested: degrees of damage of shoots, number of leaves, number of runners (stolons), yield of fresh biomass and dry matter of shoots and roots, and percentage of dry matter of shoots and roots. Tested cultivars showed signifi cant diff erences of the level of damage (necrosis and decay of plants due to high NaCl concentration). Th e highest degree of damage was shown by 'Elsanta' in both trial years, followed by 'Marmolada' and 'Miranda' respectively. Th e analysis of the number of leaves and stolons confi rmed the same trend. Th e yield of fresh biomass and dry matter of shoots and roots was signifi cantly reduced with the application of NaCl. 'Miranda' was characterised by the highest fresh biomass and dry matter yield, followed by 'Marmolada' and 'Elsanta'. Average decrease of fresh biomass yield of shoots in comparison to the control was 45, 33 and 22% in 'Elsanta', 'Marmolada' and 'Miranda'. Dry matter yield of shoots decreased correspondingly for 34, 28 and 18%. Total dry matter yield (shoots + roots) of 'Elsanta' was signifi cantly reduced at 0.5 g NaCl L-1 and of 'Marmolada' and 'Miranda' at 1.0 and 2.0 g L-1 respectively.


Kovacevic D.B.,University of Zagreb | Dragovic-Uzelac V.,University of Zagreb | Vujevic P.,Croatian Center for Agriculture | Obradovic D.,University of Zagreb
8th International Congress of Food Technologists, Biotechnologists and Nutritionists, Proceedings | Year: 2014

The quality of dried plums depends on the drying parameters, as well as the pretreatments used prior to drying. Since plums are covered naturally with a thin layer of wax, it is necessary to increase water transport from plums to drying medium. Chemical pre-treatments normally break down the waxy fruit surface and create microscopic cracks which increase moisture permeability. The best pre-treatment method for drying any fruit should optimally reduce drying time while keeping product quality high (color, nutrient content, taste, etc.). Therefore, the aim of this research was to select an appropriate chemical and thermal pre-treatment combination for plum drying at low and moderate temperatures occurring in a laboratory tray dryer to minimize the plums'quality loss. In order to select the best plum cultivar for drying four cultivars (Prunus domestica L.) President, Topend, Tophit and Topper are investigated in terms of physical and chemical parameters (total dry matter, total soluble solids, pH, total acidity) and phenolics (total phenols (TP), total flavonoids (TF) and total nonflavonoids (TNF)). Compared to other cultivars, Topend demonstrated the best characteristics for drying showing the highest phenolic content, soluble solids and lower total acidity, therefore is chosen for drying process. Topend plums were dipped in one of two different pre-treatment solutions (1% KOH or water) at two dipping temperatures (23 or 60 °C) for 1 min to accelerate the skin moisture diffusivity by breaking down the waxy cuticular surface of plum. Subsequently the plum samples were dried at low or moderate temperatures (<70 °C) and completed approximately over the 13 hours. Physical and chemical parameters are not markedly differing among plums dipped in 22 °C or 60 °C hot water. However, plums treated by 1% KOH at 60 °C dipping temperature showed higher total dry matter, pH and total acidity in comparison to plums treated by 1% KOH at 22° C dipping temperature. The pretreatment combinations caused variations in the prune phenolics, too. Obtained results indicate that dipping at 60 °C results with higher concentrations of phenolics regardless of solution type. The highest concentrations of TP and TNF were found in plums pre-treated with 1% KOH (60 °C), while the highest concentrations of TF were found in plums pre-treated with water at 22 °C. In conclusion, the experimental results show that the selection of dipping temperature and chemical solution is an important step for successful dried plum production at low and moderate air drying temperatures.


Pecek G.,Croatian Center for Agriculture | Pavlovic D.M.,Marulicev trg | Babic S.,Marulicev trg
International Journal of Environmental Analytical Chemistry | Year: 2013

A method for analysis of 20 commonly used pesticides in surface water based on solid-phase extraction and gas chromatography-mass spectrometry was proposed. During method development the key parameters that can affect SPE extraction and determination such as selection of efficient SPE sorbent, pH of water sample, type and volume of elution solvent, breakthrough volume and matrix effects were investigated. The method was validated using spring water spiked with appropriate concentration of pesticides. The obtained correlation coefficients were in range 0.9972-1.000, limits of detection (LOD) were 0.001-0.5 μg L-1 and the limits of quantification (LOQ) were 0.005-1 μg L-1 depending on a pesticide. Much higher LOD (20 μg L-1) and LOQ (50 μg L-1) values were obtained for bentazone. The influence of matrix was assessed using real water samples spiked with appropriate concentration of pesticide standards solution. Both signal enhancement and suppression were observed, depending on a pesticide, therefore standard addition method was used for pesticides determination. The developed method was applied on real water samples taken in close vicinity of agricultural fields. Many of the targeted pesticides were found in the samples and the results are presented in this article. © 2013 Copyright Taylor and Francis Group, LLC.


Dragovic-Uzelac V.,University of Zagreb | Savic Z.,Croatian Center for Agriculture | Brala A.,University of Zagreb | Levaj B.,University of Zagreb | And 2 more authors.
Food Technology and Biotechnology | Year: 2010

The aim of this study is to evaluate the content of phenolics: total phenols (TPC), flavonoids (TF), anthocyanins (TA), flavan-3-ols (TF3ols), as well as total antioxidant capacity (TAC) and reducing power (RP) in four blueberry cultivars (Vaccinium corymbosum L.) introduced in the Northwest Croatian climate conditions. Phenolic compounds were measured by spectrophotometric methods, TAC was determined using DPPH and ABTS assays and RP by FRAP assay. All cultivars contained high mass fraction of TPC, TF, TA and lower mass fraction of TF3ols. Among the researched fruits, Sierra cultivar contained the highest amounts of all groups of phenolics, followed by Elliott>Bluecrop≥Duke. Significant differences were observed in phenolic mass fraction among different cultivars and growing seasons (p<0.05), and phenolic compounds were significantly higher in growing season 2006. Examined cultivars possess high antioxidant capacity and reducing power, and all phenolics were highly correlated with TAC and RP (R=0.46 to 0.99). The study indicated that growing and climate conditions in Northwest Croatia are convenient for introducing blueberry cultivars. Generally, blueberry fruits are a rich source of phenolics, which show evident antioxidant capacity.


Masten Milek T.,Croatian Center for Agriculture | Simala M.,Institute for Plant Protection | Pintar M.,Institute for Plant Protection
EPPO Bulletin | Year: 2016

In recent years, several species of alien scale insects have arrived and spread rapidly through European and Mediterranean countries. One hundred and twenty-nine species of alien scale insects have so far been recorded in Europe. This paper presents a list of alien scale insect species for Croatia, comprising 56 species from 8 different families. Four of them are new records for Croatia: Aonidiella taxus Leonardi, 1906, Ichnaspis longirostris (Signoret, 1882), Phoenicococcus marlatii (Cockerell, 1899) and Spilococcus mammillariae (Bouche, 1844). The majority of species recorded belong to the following families: Diaspididae (28 species), Coccidae (12 species), Pseudococcidae (10 species), Eriococcidae (two species). The scale insect families Dactylopidae, Margarodidae, Ortheziidae and Pheniococcidae comprised only one recorded alien species each. During the time frame covered in the review (from the beginning of the 20th century until 2015), the majority of alien scale insect species (23) were registered in the period from 2000 to 2015. Data on the origin of introduced alien scale insects shows that the majority of the species originate from Asia (22 species), followed by North America (6 species), Africa, Australia and Central America (5 species each) and South America (4 species), with 9 species being of unknown origin. © 2016 The Authors. Journal compilation © 2016 OEPP/EPPO


Cupic T.,Osijek Agricultural Institute | Popovic S.,Osijek Agricultural Institute | Tucak M.,Osijek Agricultural Institute | Jukic G.,Croatian Center for Agriculture | Rukavina I.,Croatian Center for Agriculture
Journal of Central European Agriculture | Year: 2013

The field pea breeding at the Agricultural Institute Osijek has primarily been aimed at continued development of new cultivar types with high yield, potential early group maturity and resistance to lodging. Letin is a new semi-leafless -winter field pea (Pisum sativum) with purple flowers and a mottled light brown seed coat. Semi-leafless and leafed genotypes were evaluated for plant height, lodging, maturity, forage yield and quality in two locations. The forage yield of pea genotypes has an average of 32.21 t*ha-1. Higher yield was obtained from semi-leafless genotype (34.48 t*ha-1). The leafed genotype has achieved better nutritive characteristics. The obtained results point to a need for new tests and the possible introduction of semi-leafless cultivars in the production as a pea-cereal mixture or as pure crops to be utilized by cutting.

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