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Joshna P.,Criya Biolabs | Anuradha P.,Criya Biolabs | Jyothi K.,Criya Biolabs | Vinathi D.,Criya Biolabs | Jyothi G.,Criya Biolabs
Research Journal of Biotechnology | Year: 2011

The present study was undertaken with the primary objective, amplification of DNA isolated from blood samples of cattle breeds in Holstein Friesian calf and NDL cross breeds. Blood samples were collected in vacutainers containing EDTA as an anticoagulant from cattle breeds. Genomic DNA was extracted from blood samples using phenol: chloroform method. The quality of DNA was assessed by UV spectrophotometry. PCR was carried out in a final reaction volume of 25ul reaction mixture. Increase in the stringency during initial cycles of PCR reaction increased the sharpness and brightness of the bands. DNA samples were electrophoresed on polyacrylamide gel and observed as the DNA pattern by silver staining. Milk protein genes (β- lactoglobulin) could be useful as genetic markers for additional selection criteria in dairy cattle breeding. The polymorphism of β-lactoglobulin gene was identified by conducting PCR studies.

Anuradha P.,Criya Biolabs | Jhansi Rani D.,Criya Biolabs | Abid Pasha S.,Criya Biolabs | Kamakshi S.,Criya Biolabs
Biosciences Biotechnology Research Asia | Year: 2010

The antimicrobial properties of Indian antidiabetic plant extracts of Syzygium cumini, Azadirachta indica and Bougainvillea spectabilis, against six bacterial strains (Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa and Proteus vulgaris). The collected antidiabetic plants Syzygium cumini, Azadirachta indica, and Bougainvillea spectabilis were used as hypoglycemic plants in West Indies and some parts of Asia. Chloroform, ethanol and aqueous extracts were prepared sequentially from leaves of these plants. It was observed that methanol and ethanolic extracts of Syzygium cumini have significant activity against gram positive bacteria. Klebsiella pneumonia and Proteus vulgaris were more sensitive to the extracts of Azadirachta indica and Bougainvillea spectabilis respectively.

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