Viana K.A.,Prefeitura de Belo Horizonte |
Avelar R.S.,Centro Universitario Newton Paiva |
Avelar D.M.V.,Laboratorio Of Patologia Clinica Sao Paulo |
Carvalho B.,Servico de Hematologia da Fundacao Hemominas |
And 4 more authors.
Jornal Brasileiro de Patologia e Medicina Laboratorial | Year: 2014
Introduction: Currently, the reticulocyte counting is a challenge for clinical laboratories in Brazil, mainly for the ordinary ones, which still use the manual method. This method has some limitations, since it consists of a laborious method, time consuming, with low accuracy. Objectives: This study has developed and evaluated the performance of a New Laboratory Protocol for flow cytometry (FC) reticulocytes counting using acridine orange (AO) as dye, aiming to standardize a more precise, easy, fast implementation, and low cost protocol. After standardization of the New Protocol (FC/AO), it was compared with the manual method. The results were analyzed according to the recommendations of the National Committee for Clinical Laboratory Standards (NCCLS), now known as Clinical and Laboratory Standards Institute (CLSI), to evaluate the interchangeability of methods in linear regression analysis and paired t test, besides other quality control tests. Conclusion: Based on these results concerning to the correlation between the methods and the tests related to quality control, we can admit that FC/AO for reticulocyte counting shows undeniable advantages when compared to the preexisting manual method.
Confalonieri U.E.C.,CPqRR Fiocruz |
Lima A.C.L.,CPqRR Fiocruz |
Brito I.,CPqRR Fiocruz |
Quintao A.F.,ESP MG
Climatic Change | Year: 2014
A regional vulnerability study in relation to the projected patterns of climate change (A2 and B2 scenarios) was developed for the Brazilian Northeastern region. An aggregated Vulnerability Index was constructed for each of the nine States of the region, based on the following information: population projections; climate-induced migration scenarios; disease trends; desertification rates; economic projections (GDP and employment) and projections for health care costs. The results obtained shall subsidize public policies for the protection of the human population from the projected impacts of regional changes in climate patterns. © 2013, Springer Science+Business Media Dordrecht.