CP Bio Inc.

Murrieta Hot Springs, CA, United States

CP Bio Inc.

Murrieta Hot Springs, CA, United States
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Guo Y.,Nanjing Agricultural University | Zhu C.,Nanjing Agricultural University | Gan L.,Nanjing Agricultural University | Ng D.,CP Bio Inc. | Xia K.,Nanjing Agricultural University
PLoS ONE | Year: 2015

Gibberellins (GA) regulate various components of plant development. Iron and Mn plaque result from oxiding and hydroxiding Fe and Mn, respectively, on the roots of aquatic plant species such as rice (Oryza sativa L.). In this study, we found that exogenous gibberellic acid3 (GA3) spray decreased Fe plaque, but increased Mn plaque, with applications of Kimura B nutrient solution. Similar effects from GA3, leading to reduced Fe plaque and increased Mn plaque, were also found by scanning electron microscopy and energy dispersive X-ray spectrometric microanalysis. Reduced Fe plaque was observed after applying GA3 to the groups containing added Fe2+ (17 and 42 mg·L-1) and an increasing trend was detected in Mn plaques of the Mn2+ (34 and 84 mg·L-1) added treatments. In contrast, an inhibitor of GA3, uniconazole, reversed the effects of GA3. The uptake of Fe or Mn in rice plants was enhanced after GA3 application and Fe or Mn plaque production. Strong synergetic effects of GA3 application on Fe plaque production were detected. However, no synergetic effects on Mn plaque production were detected. © 2015 Xia et al.


Yang W.,Nanjing Agricultural University | Zhu C.,Nanjing Agricultural University | Ma X.,Nanjing Agricultural University | Li G.,Nanjing Agricultural University | And 3 more authors.
PLoS ONE | Year: 2013

In plants, salicylic acid (SA) is a signaling molecule that regulates disease resistance responses, such as systemic acquired resistance (SAR) and hypertensive response (HR). SA has been implicated as participating in various biotic and abiotic stresses. This study was conducted to investigate the role of SA in adventitious root formation (ARF) in mung bean (Phaseolus radiatus L) hypocotyl cuttings. We observed that hypocotyl treatment with SA could significantly promote the adventitious root formation, and its effects were dose and time dependent. Explants treated with SA displayed a 130% increase in adventitious root number compared with control seedlings. The role of SA in mung bean hypocotyl ARF as well as its interaction with hydrogen peroxide (H 2O2) were also elucidated. Pretreatment of mung bean explants with N, N'-dimethylthiourea (DMTU), a scavenger for H2O 2, resulted in a significant reduction of SA-induced ARF. Diphenyleneiodonium (DPI), a specific inhibitor of membrane-linked NADPH oxidase, also inhibited the effect of adventitious rooting triggered by SA treatment. The determination of the endogenous H2O2 level indicated that the seedlings treated with SA could induce H2O 2 accumulation compared with the control treatment. Our results revealed a distinctive role of SA in the promotion of adventitious rooting via the process of H2O2 accumulation. This conclusion was further supported by antioxidant enzyme activity assays. Based on these results, we conclude that the accumulation of free H2O2 might be a downstream event in response to SA-triggered adventitious root formation in mung bean seedlings. © 2013 Yang et al.


Guo Y.,Nanjing Agricultural University | Zhu C.,Nanjing Agricultural University | Gan L.,Nanjing Agricultural University | Ng D.,CP Bio Inc. | Xia K.,Nanjing Agricultural University
Plant Growth Regulation | Year: 2015

Flooding is one of the most important abiotic constraints on rice yields in rain-fed lowlands. During the submergence period, ethylene accumulates rapidly to a physiologically active level in the tissues of submerged plants. Also, iron and manganese plaque formation on roots is an adaptation that allows rice-like plant species to survive in their natural aquatic habitats. This study provides evidence of a time-dependent increase in the iron and manganese plaque content of rice root surfaces during 96 h of complete submergence (CS), accompanied by the increased expression in roots of genes in the OsACS (OsACS1, OsACS2, OsACS4 and OsACS5) and OsACO (OsACO1, OsACO2, OsACO3 and OsACO7) families. To further investigate ethylene action in root plaque formation, rice seedlings were completely submerged in Kimura B nutrient solutions containing different concentrations of Fe2+ or Mn2+. Under these conditions, root plaques were induced dramatically in an almost dose-dependent manner. Iron and manganese plaque formation was promoted by the ethylene precursor 1-aminocyclopropane-1-carboxylic acid and blocked by the ethylene biosynthesis inhibitor aminoethoxyvinyl glycine (AVG) in seedlings grown on media supplemented or not with Fe2+ or Mn2+. Furthermore, submergence-induced iron and manganese root plaque formation was decreased by pretreatment with 5 μM AVG for 12 h. Our findings suggest that ethylene signaling is involved in CS-induced iron and manganese root plaque formation in rice. © 2014, Springer Science+Business Media Dordrecht.


PubMed | CP Bio Inc. and Nanjing Agricultural University
Type: Journal Article | Journal: PloS one | Year: 2015

Gibberellins (GA) regulate various components of plant development. Iron and Mn plaque result from oxiding and hydroxiding Fe and Mn, respectively, on the roots of aquatic plant species such as rice (Oryza sativa L.). In this study, we found that exogenous gibberellic acid3 (GA3) spray decreased Fe plaque, but increased Mn plaque, with applications of Kimura B nutrient solution. Similar effects from GA3, leading to reduced Fe plaque and increased Mn plaque, were also found by scanning electron microscopy and energy dispersive X-ray spectrometric microanalysis. Reduced Fe plaque was observed after applying GA3 to the groups containing added Fe2+ (17 and 42 mgL(-1)) and an increasing trend was detected in Mn plaques of the Mn2+ (34 and 84 mgL(-1)) added treatments. In contrast, an inhibitor of GA3, uniconazole, reversed the effects of GA3. The uptake of Fe or Mn in rice plants was enhanced after GA3 application and Fe or Mn plaque production. Strong synergetic effects of GA3 application on Fe plaque production were detected. However, no synergetic effects on Mn plaque production were detected.


PubMed | CP Bio Inc. and Nanjing Agricultural University
Type: Journal Article | Journal: PloS one | Year: 2014

In plants, salicylic acid (SA) is a signaling molecule that regulates disease resistance responses, such as systemic acquired resistance (SAR) and hypertensive response (HR). SA has been implicated as participating in various biotic and abiotic stresses. This study was conducted to investigate the role of SA in adventitious root formation (ARF) in mung bean (Phaseolus radiatus L) hypocotyl cuttings. We observed that hypocotyl treatment with SA could significantly promote the adventitious root formation, and its effects were dose and time dependent. Explants treated with SA displayed a 130% increase in adventitious root number compared with control seedlings. The role of SA in mung bean hypocotyl ARF as well as its interaction with hydrogen peroxide (H2O2) were also elucidated. Pretreatment of mung bean explants with N, N-dimethylthiourea (DMTU), a scavenger for H2O2, resulted in a significant reduction of SA-induced ARF. Diphenyleneiodonium (DPI), a specific inhibitor of membrane-linked NADPH oxidase, also inhibited the effect of adventitious rooting triggered by SA treatment. The determination of the endogenous H2O2 level indicated that the seedlings treated with SA could induce H2O2 accumulation compared with the control treatment. Our results revealed a distinctive role of SA in the promotion of adventitious rooting via the process of H2O2 accumulation. This conclusion was further supported by antioxidant enzyme activity assays. Based on these results, we conclude that the accumulation of free H2O2 might be a downstream event in response to SA-triggered adventitious root formation in mung bean seedlings.


Trademark
Cp Bio Inc. | Date: 2012-08-01

Fertilizers for agricultural use.


Trademark
Cp Bio Inc. | Date: 2011-03-23

Fertilizers; Fertilizers for agricultural use.


Trademark
Cp Bio Inc. | Date: 2011-03-25

Fertilizers.

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