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Timişoara, Romania

Maharaj S.V.M.,U.S. Geological Survey | Maharaj S.V.M.,Center for Research on Environmental Medicine | Orem W.H.,U.S. Geological Survey | Tatu C.A.,U.S. Geological Survey | And 3 more authors.
Environmental Geochemistry and Health | Year: 2014

The Pliocene lignite hypothesis is an environmental hypothesis that has been proposed to explain the etiology of Balkan endemic nephropathy (BEN). Aqueous leaching experiments were conducted on a variety of coal samples in order to simulate groundwater leaching of organic compounds, and to further test the role of the Pliocene lignite hypothesis in the etiology of BEN. Experiments were performed on lignite coal samples from endemic BEN areas in Romania and Serbia, and lignite and bituminous coals from nonendemic regions in Romania and the USA. Room temperature, hot water bath, and Soxhlet aqueous extraction experiments were conducted between 25 and 80 °C, and from 5 to 128 days in duration. A greater number of organic compounds and in higher concentrations were present in all three types of leaching experiments involving endemic area Pliocene lignite samples compared to all other coals examined. A BEN causing molecule or molecules may be among phenols, PAHs, benzenes, and/or lignin degradation compounds. The proposed transport pathway of the Pliocene lignite hypothesis for organic compound exposure from endemic area Pliocene lignite coals to well and spring drinking water, is likely. Aromatic compounds leached by groundwater from Pliocene lignite deposits in the vicinity of endemic BEN areas may play a role in the etiology of the disease. A better understanding of organic compounds leached by groundwater from Pliocene lignite deposits may potentially lead to the identification and implementation of effective strategies for the prevention of exposure to the causative agent(s) for BEN, and in turn, prevention of the disease. © 2013 The Author(s). Source

Dehelean C.A.,Victor Babes University of Medicine and Pharmacy Timisoara | Soica C.M.,Victor Babes University of Medicine and Pharmacy Timisoara | Toma C.-C.,Vasile Goldis Western University of Arad | Feflea S.,Victor Babes University of Medicine and Pharmacy Timisoara | And 2 more authors.
Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii | Year: 2010

Betulin is a triterpene with an important therapeutic activity even on tumour treatment. It is very low soluble in water and biological environment. The increasing of its solubility by cyclodextrin complexation could lead to an improvement in the biological activity. Materials and methods used betulin and 6-O-maltosyl-beta-cyclodextrin and a simple kneading procedure. The compound and final product were evaluated by SEM pictures, in vitro tests on A431 cell line and on an embryonated egg model. Results and discussions indicated the important activity of betulin as antitumor agent and an antiangiogenic activity. The mixture with the ramified cyclodextrin seems to be a complex formation because of the shape of compounds changes. The complexes had a better biological activity. The main conclusion is that betulin is an important antitumor compound and increasing of its solubility could improve the biological activity. © 2010 Vasile Goldiş University Press. Source

Gazinska P.,Kings College London | Herman D.,County Hospital Timisoara | Gillett C.,Kings College London | Pinder S.,Kings College London | Mantle P.,Imperial College London
Toxins | Year: 2012

Ochratoxin A (OTA) is considered to be a possible human urinary tract carcinogen, based largely on a rat model, but no molecular genetic changes in the rat carcinomas have yet been defined. The phosphorylated-S6 ribosomal protein is a marker indicating activity of the mammalian target of rapamycin, which is a serine/threonine kinase with a key role in protein biosynthesis, cell proliferation, transcription, cellular metabolism and apoptosis, while being functionally deregulated in cancer. To assess p-S6 expression we performed immunohistochemistry on formalin-fixed and paraffin-embedded tumours and normal tissues. Marked intensity of p-S6 expression was observed in highly proliferative regions of rat renal carcinomas and a rare angiosarcoma, all of which were attributed to prolonged exposure to dietary OTA. Only very small OTA-generated renal adenomas were negative for p-S6. Examples of rat subcutaneous fibrosarcoma and testicular seminoma, as well as of normal renal tissue, showed no or very weak positive staining. In contrast to the animal model, human renal cell carcinoma, upper urinary tract transitional cell carcinoma from cases of Balkan endemic nephropathy, and a human angiosarcoma were negative for p-S6. The combined findings are reminiscent of constitutive changes in the rat tuberous sclerosis gene complex in the Eker strain correlated with renal neoplasms, Therefore rat renal carcinogenesis caused by OTA does not obviously mimic human urinary tract tumourigenesis. © 2012 by the authors; licensee MDPI, Basel, Switzerland. Source

Mantle P.G.,Imperial College London | Amerasinghe C.,Ealing Hospital | Brown A.L.,Kings College London | Herman D.,County Hospital Timisoara | And 5 more authors.
Toxins | Year: 2010

DNA ploidy measurement has been applied uniquely to wax-embedded tissue of primary renal cell and metastatic tumours of a key experimental researcher on porcine ochratoxicosis, a control, and four transitional cell carcinomas from cases of Balkan endemic nephropathy. Primary renal tumour was diploid, and hyperdiploid metastasis was within the lower ploidy range for typical renal cell carcinoma. Three Balkan primary tumours showed extensive aneuploidy indicating marked nuclear instability, similar to model rat renal carcinoma caused by ochratoxin A. In contrast, much less nuclear instability in the putative occupational ochratoxicosis case fitted poorly with the ochratoxin A model. © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. Source

Pirtea L.,Victor Babes University of Medicine and Pharmacy Timisoara | Grigoras D.,Victor Babes University of Medicine and Pharmacy Timisoara | Matusz P.,Victor Babes University of Medicine and Pharmacy Timisoara | Pirtea M.,County Hospital Timisoara | And 6 more authors.
Canadian Journal of Infectious Diseases and Medical Microbiology | Year: 2016

Background. Persistent human papillomavirus (HPV) infection is a necessary event in cervical cancer tumorigenesis. Our objectives were to estimate the rate of HPV infection persistence after large loop excision of the transformation zone (LEEP) in patients with high grade squamous intraepithelial lesions (HSIL) and to investigate if HPV persistence is type related. Methods. We conducted a prospective study on 89 patients with HSIL treated with LEEP. DNA HPV was performed before surgery and at 6, 12, and 18 months after LEEP. Results. Four patients were excluded from the study. The HPV persistence in the remaining 85 patients was 32.95% (6 months), 14.12% (12 months), and 10.59% (18 months). Type 16 had the highest persistence rate, 23.5% (6 months), 11.8% (12 months), and 8.2% (18 months). Coinfection was found to be 54.12% before LEEP and 18.8% (6 months), 4.7% (12 months), and 3.5% (18 months) after LEEP. The rate of coinfections including type 16 was 46.83% of all coinfections. Coinfection including type 16 was not correlated with higher persistence rate compared to infection with type 16 only. Conclusions. HPV infection is not completely eradicated by LEEP in patients with HSIL lesion on PAP smear. HPV persistence after LEEP is influenced by HPV type. HPV type 16 has the highest persistence rate. © 2016 Laureniu Pirtea et al. Source

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