Council for Scientific and Industrial Research Oil Palm Research Institute CSIR OPRI

Sekondi-Takoradi, Ghana

Council for Scientific and Industrial Research Oil Palm Research Institute CSIR OPRI

Sekondi-Takoradi, Ghana
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Rosete Y.A.,Sporometrics Toronto | Diallo H.A.,Nangui Abrogoua University | Yankey N.,Council for Scientific and Industrial Research Oil Palm Research Institute CSIR OPRI | Saleh M.,Sporometrics Toronto | And 5 more authors.
Annals of Applied Biology | Year: 2017

Surveys for the Côte d'Ivoire lethal yellowing (CILY) phytoplasma were conducted in eight severely CILY-affected villages of Grand-Lahou in 2015. Leaves, inflorescences and trunk borings were collected from coconut palms showing CILY symptoms and from symptomless trees. Total DNA was extracted from these samples and tested by nested polymerase chain reaction/RFLP and sequence analysis of the 16S rRNA, ribosomal protein (rp) and the translocation protein (secA) genes. The CILY phytoplasma was detected in 82.9% of the symptom-bearing palms collected from all the surveyed villages and from all the plant parts. Trunk borings were recommended as the most suitable plant tissue type for sampling. Results indicate that the CILY phytoplasma may have a westward spread to other coconut-growing areas of Grand-Lahou. CILY phytoplasma strains infecting coconut palms in the western region of Grand-Lahou exhibited unique single nucleotide polymorphisms on the rp sequence compared to the strains from the eastern region. Moreover, single nucleotide polymorphisms on the SecA sequence distinguished the CILY phytoplasma from the Cape St. Paul Wilt Disease phytoplasma in Ghana, and the Lethal Yellowing phytoplasma in Mozambique. © 2017 Association of Applied Biologists.


Yankey E.N.,University of Nottingham | Yankey E.N.,Council for Scientific and Industrial Research Oil Palm Research Institute CSIR OPRI | Swarbrick P.,University of Nottingham | Dickinson M.,University of Nottingham | And 4 more authors.
Bulletin of Insectology | Year: 2011

Accurate and timely detection is important for the control of lethal disease of coconut in Ghana. To improve on the detection of the phytoplasmas involved, multiplex PCR with an in-built internal control and a real-time loop mediated isothermal amplification (LAMP) were used to eliminate false negative results and minimise cross-over contamination. Real-time LAMP provided a fast and reliable means of diagnosis.


Pilet F.,CIRAD - Agricultural Research for Development | Poulin L.,CIRAD - Agricultural Research for Development | Nkansah-Poku J.,Council for Scientific and Industrial Research Oil Palm Research Institute CSIR OPRI | Quaicoe R.N.,Council for Scientific and Industrial Research Oil Palm Research Institute CSIR OPRI
Bulletin of Insectology | Year: 2011

In Ghana, coconut lethal yellowing phytoplasma locally called Cape Saint Paul Wilt Disease (CSPWD) is the most damaging coconut disease. Two different foci of the disease can be distinguished: the first one covering the coast of the Western and Central Regions, and the second covering the coast of the Volta region. To test the hypothesis of a genetic differentiation between the CSPWD phytoplasma from the two foci, the partial ribosomal operon 16S, and two ribosomal protein genes (rplV and rpsC) of 14 strains were sequenced. The ribosomal protein gene sequences allowed the differentiation of the strains originating from the two different foci by a unique SNP, confirming a genetic differentiation.


Yankey E.N.,Council for Scientific and Industrial Research Oil Palm Research Institute CSIR OPRI | Swarbrick P.J.,University of Nottingham | Swarbrick P.J.,CABI Inc | Nipah J.O.,Council for Scientific and Industrial Research Oil Palm Research Institute CSIR OPRI | And 2 more authors.
Journal of Plant Pathology | Year: 2014

Lethal Yellowing-type diseases of coconut have destroyed thousands of coconut palms globally, and in Ghana the disease [Cape St. Paul wilt disease (CSPWD)] is considered as the single most important factor affecting the coconut industry. To improve the diagnostics available for CSPW detection, a ribosomal and a non-ribosomal- based assay were assessed for their effectiveness in diagnosing CSPWD. PCR diagnoses and observation of symptom development were carried out twice per year over three years to study the fate of infected palms and the pattern of disease spread. Comparison of the effectiveness of different PCR assays at detecting CSPWD-infection showed a comparable performance of a single round secA-based assay to nested PCR using primers targeting ribosomal DNA sequences. The study showed that all infected palms eventually died from infection and analysis of the location of palms becoming infected showed no clear pattern in the spread of infection.

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